spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X12575; SV 1; linear; genomic DNA; STD; PRO; 1720 BP.
XX
AC   X12575;
XX
DT   30-JAN-1989 (Rel. 18, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Caldocellum saccharolyticum bglA gene for beta-glucosidase
XX
KW   beta-glucosidase; bglA gene.
XX
OS   Caldicellulosiruptor saccharolyticus
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor.
XX
RN   [1]
RP   1-1720
RX   DOI; 10.1007/BF00333402
RX   PUBMED; 2851713.
RA   Love D.R., Bergquist P.L.;
RT   "Sequence structure and expression of a cloned beta-glucosidase gene from
RT   an extreme thermophile";
RL   Mol. Gen. Genet. 213(1):84-92(1988).
XX
CC   Caldocellum saccharolyticum is a extreme thermophile and appears
CC   to be a Gram-positive anaerobic bacterium.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1720
FT                   /organism="Caldicellulosiruptor saccharolyticus"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="lambda 1059."
FT                   /db_xref="taxon:44001"
FT   promoter        7..12
FT                   /note="put.-10 region"
FT   RBS             20..25
FT                   /note="put.rRNA-binding site"
FT   CDS             29..1396
FT                   /transl_table=11
FT                   /note="beta-glucosidase (AA 1-455)"
FT                   /db_xref="GOA:P10482"
FT                   /db_xref="InterPro:IPR001360"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="InterPro:IPR017736"
FT                   /db_xref="InterPro:IPR017853"
FT                   /db_xref="InterPro:IPR018120"
FT                   /db_xref="UniProtKB/Swiss-Prot:P10482"
FT                   /protein_id="CAA31087.1"
FT                   /translation="MDMSFPKGFLWGAATASYQIEGAWNEDGKGESIWDRFTHQKRNIL
FT                   YGHNGDVACDHYHRFEEDVSLMKELGLKAYRFSIAWTRIFPDGFGTVNQKGLEFYDRLI
FT                   NKLVENGIEPVVTLYHWDLPQKLQDIGGWANPEIVNYYFDYAMLVINRYKDKVKKWITF
FT                   NEPYCIAFLGYFHGIHAPGIKDFKVAMDVVHSLMLSHFKVVKAVKENNIDVEVGITLNL
FT                   TPVYLQTERLGYKVSEIEREMVSLSSQLDNQLFLDPVLKGSYPQKLLDYLVQKDLLDSQ
FT                   KALSMQQEVKENFIFPDFLGINYYTRAVRLYDENSSWIFPIRWEHPAGEYTEMGWEVFP
FT                   QGLFDLLIWIKESYPQIPIYITENGAAYNDIVTEDGKVHDSKRIEYLKQHFEAARKAIE
FT                   NGVDLRGYFVWSLMDNFEWAMGYTKRFGIIYVDYETQKRIKKDSFYFYQQYIKENS"
XX
SQ   Sequence 1720 BP; 555 A; 308 C; 350 G; 507 T; 0 other;
     aagctttatt atccttaaaa ggaggtttat ggacatgagt ttcccaaaag gatttttgtg        60
     gggtgctgca actgcgtcat atcagattga aggtgcatgg aacgaagatg gaaaaggcga       120
     atctatttgg gacaggttca cacatcaaaa aagaaatatt ctatacggtc ataatggcga       180
     tgttgcctgc gaccattacc acaggttcga agaagatgtt tctcttatga aagaacttgg       240
     actcaaagcc tacaggtttt ccattgcatg gacaagaatc tttccagatg gttttggtac       300
     tgtgaatcag aaaggtcttg agttttatga tagacttatc aacaaacttg ttgaaaacgg       360
     tattgaaccg gttgtcaccc tttaccactg ggaccttccg cagaagctgc aagacattgg       420
     cggctgggca aacccagaaa ttgtgaatta ttatttcgat tatgcaatgc ttgttataaa       480
     ccgttataaa gacaaagtaa aaaagtggat aacattcaat gaaccttatt gcattgcttt       540
     tttaggctac tttcatggaa tccatgcacc aggaataaaa gatttcaaag ttgcaatgga       600
     tgttgtgcac agcctcatgc tttctcattt taaggttgta aaagctgtaa aagaaaacaa       660
     tattgatgtt gaggtgggaa ttacattaaa tttaacccca gtctaccttc agacagaacg       720
     acttggatat aaggtaagcg aaattgaaag agaaatggtt tctttgagca gccagcttga       780
     caaccaactt ttccttgatc cagtacttaa aggaagctat ccacaaaagc tgttagatta       840
     tcttgttcaa aaagatttgt tagatagcca aaaagcattg agcatgcagc aggaagtaaa       900
     agaaaatttc atcttccctg attttcttgg tatcaactat tacacacgtg ccgtcaggct       960
     ttacgacgaa aattctagct ggatatttcc aataagatgg gaacatcctg caggagaata      1020
     caccgagatg ggctgggaag tgttcccgca ggggcttttc gatcttttga tttggattaa      1080
     agaaagttac ccacaaattc caatttatat aacagaaaac ggtgctgctt ataacgatat      1140
     tgtaactgaa gatggaaaag tgcacgatag caaaaggatt gagtatttaa aacagcactt      1200
     tgaagcagca agaaaggcaa ttgaaaatgg tgtggatttg cgaggctatt ttgtgtggtc      1260
     tttgatggac aattttgaat gggcaatggg ttatacaaaa aggtttggaa ttatatatgt      1320
     ggattatgaa acccaaaaga gaatcaaaaa agacagcttc tatttttatc agcagtatat      1380
     aaaggaaaat tcgtaaatat aaataaaggc gggcttttgg agaaattcat tttctcaaaa      1440
     gcccgttttc tattttccat ccttcatgtg aaagtagcca aatctttttt tcaagcccac      1500
     cgctaaaccc acctaaggac aaatctgact tgaccacccg atggcatgga acaattatca      1560
     cagctggatt tttgccaaca gcatttccaa cagctcttgc gccctgtggt tttccaactt      1620
     tttctgcaag ctctctgtaa gaaattacac ttctaaatgg aacttttata agttcattcc      1680
     aaactctttt ttgaaactcc gtcccctgca gctgaagctt                            1720
//
spacer
spacer