 |
|
EBI DbfetchID X12455; SV 1; linear; genomic DNA; STD; PRO; 2142 BP. XX AC X12455; XX DT 22-MAR-1989 (Rel. 19, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 6) XX DE A. vinelandii gene for dihydrolipoyltransacetylase, E2 component of DE pyruvate dehydrogenase complex (EC 2.3.1.12) XX KW dihydrolipoamide acetyltransferase; pyruvate dehydrogenase. XX OS Azotobacter vinelandii OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Azotobacter. XX RN [1] RP 1-2142 RX DOI; 10.1111/j.1432-1033.1988.tb14140.x RX PUBMED; 3292237. RA Hanemaaijer R., Janssen A., de Kok A., Veeger C.; RT "The dihydrolipoyltransacetylase component of the pyruvate dehydrogenase RT complex from Azotobacter vinelandii. Molecular cloning and sequence RT analysis"; RL Eur. J. Biochem. 174(4):593-599(1988). XX CC Data kindly reviewed (21-Apr-1989) by De Kok A. XX FH Key Location/Qualifiers FH FT source 1..2142 FT /organism="Azotobacter vinelandii" FT /strain="ATCC478" FT /mol_type="genomic DNA" FT /clone_lib="pUC9." FT /db_xref="taxon:354" FT CDS <1..140 FT /codon_start=3 FT /transl_table=11 FT /product="pyruvate dehydrogenase (46 AA)" FT /db_xref="GOA:P10801" FT /db_xref="HSSP:1L8A" FT /db_xref="InterPro:IPR015941" FT /db_xref="UniProtKB/Swiss-Prot:P10801" FT /protein_id="CAA30986.1" FT /translation="EVDRYWVVLAALEALADRGDIEAKVVAEAIAKFGIDPDKRNPLDC FT " FT RBS 140..144 FT /note="pot. ribosome binding site" FT CDS 152..2068 FT /transl_table=11 FT /product="dihydrolipoyltransacetylase (AA 1 - 638)" FT /db_xref="GOA:P10802" FT /db_xref="InterPro:IPR004167" FT /db_xref="PDB:1DPB" FT /db_xref="UniProtKB/Swiss-Prot:P10802" FT /protein_id="CAA30987.1" FT /translation="MSEIIRVPDIGGDGEVIELLVKTGDLIEVEQGLVVLESAKASMEV FT PSPKAGVVKSVSVKLGDKLKEGDAIIELEPAAGAAAAPAEAAAVPAAPTQAVDEAEAPS FT PGASATPAPAAASQEVRVPDIGSAGKARVIEVLVKAGDQVQAEQSLIVLESDKASMEIP FT SPASGVVESVAIQLNAEVGTGDLILTLRTTGAQAQPTAPAAAAAASPAPAPLAPAAAGP FT QEVKVPDIGSAGKARVIEVLVKAGDQVQAEQSLIVLESDKASMEIPSPAAGVVESVAVQ FT LNAEVGTGDQILTLRVAGAAPSGPRARGSPGQAAAAAAAAPAPAPVGAPSRNGAKVHAG FT PAVRQLAREFGVELAAINSTGPRGRILKEDVQAYVKAMMQKAKEAPAAGAASGAGIPPI FT PPVDFAKYGEIEEVPMTRLMQIGATNLHRSWLNVPHVTQFESADITELEAFRVAQKAVA FT EKAGVKLTVLPLLLKACAYLLKELPDFNSSLAPSGQALIRKKYVHIGFAVDTPDGLLVP FT VIRNVDQKSLLQLAAEAAELAEKARSKKLGADAMQGACFTISSLGHIGGTAFTPIVNAP FT EVAILGVSKASMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLGDLLADIRA FT ILL" FT repeat_region 2076..2085 FT /note="inverted repeat A" FT repeat_region 2095..2104 FT /note="inverted repeat A'" FT repeat_region 2111..2118 FT /note="inverted repeat B" FT repeat_region 2125..2132 FT /note="inverted repeat B'" XX SQ Sequence 2142 BP; 358 A; 774 C; 722 G; 288 T; 0 other; tcgaagtgga ccgctactgg gttgtgctgg ccgcgctgga agcgctggcc gatcgtggcg 60 acatcgaagc caaggtggtg gccgaagcca tcgccaagtt cggcatcgat cccgacaagc 120 gcaaccccct ggactgctga ggagagccat cgtgagcgaa atcatccgcg tacccgatat 180 cggcggcgat ggggaagtca tcgaattgct ggtcaagacc ggcgacctca tcgaggtcga 240 gcaggggctg gtggtgctgg agtccgccaa ggcgagcatg gaagttccca gtcccaaggc 300 cggagtggtc aagagcgtga gcgtcaagct gggcgacaag ctcaaggaag gcgacgcgat 360 catcgagctg gaacctgccg ccggtgccgc ggcggcacct gccgaagcgg cggccgtgcc 420 cgccgcgccg acccaggccg tcgacgaggc cgaggcgccc tctcccggcg cctccgccac 480 gcccgcgccg gccgccgcca gccaggaggt ccgggttccc gacatcggct cggccggcaa 540 ggcacgggtc atcgaggtgc tggtcaaggc cggcgaccag gtccaggccg agcagtcgct 600 gatcgtgctg gagtccgaca aggccagcat ggagatcccc tccccggctt ccggagtggt 660 ggaaagcgtc gccatccagt tgaacgccga ggtcggcacc ggcgacctga tcctcaccct 720 gcgcaccacc ggcgcccagg cccagcctac ggcgcccgcc gcggccgcgg ccgcgagccc 780 ggcacccgct ccgctggctc cggccgccgc cggtccccag gaagtcaagg tcccggacat 840 cggctcggcc ggcaaggcac gggtcatcga ggtgctggtc aaggccggcg accaggtcca 900 ggccgaacag tcgctgatcg tgctggaatc cgacaaggcc agcatggaga tcccctcccc 960 ggccgccggg gtggtggaaa gcgtcgccgt ccagttgaac gccgaggtcg gcaccggcga 1020 tcagatcctc accctgcgcg tcgccggcgc cgcgccgagc ggcccccgcg cccgcggcag 1080 ccccggccaa gccgccgccg ctgcggccgc tgccccggct ccggccccgg tcggcgcacc 1140 gagccgcaac ggcgccaagg tgcatgccgg ccccgccgtg cgccaactgg cccgcgagtt 1200 cggcgtcgaa ctggcggcga tcaacagcac cggtccgcgc gggcgcatcc tcaaggagga 1260 cgtgcaggcc tacgtcaagg cgatgatgca gaaggccaag gaggcgccgg ccgccggcgc 1320 ggccagcggc gccggcatcc cgccgattcc gccggtcgac ttcgccaagt acggcgaaat 1380 cgaagaagtg ccgatgactc gcctgatgca gatcggcgcg accaacctgc accgcagttg 1440 gctgaacgtg ccgcacgtga cccagttcga gtcggccgat atcaccgagc tggaagcctt 1500 ccgcgtcgcg cagaaggccg tcgccgagaa ggccggggtc aagctgaccg tgctgccgct 1560 gctgctcaag gcctgcgcct acctgctcaa ggagctgccg gacttcaaca gctccctggc 1620 acccagcggc caggcgctga tccgcaagaa gtacgtgcac atcggcttcg ccgtggacac 1680 cccggacggc ctgctggtgc cggtaatccg caacgtcgac cagaagagcc tgctgcaact 1740 ggccgccgag gccgccgaac tggcggagaa ggcgcgcagc aagaagctcg gcgccgacgc 1800 catgcagggt gcctgcttca ccatctccag cctcggccac atcggcggca cggccttcac 1860 gccgatcgtc aacgccccgg aagtggcgat cctcggcgtg tccaaggcca gcatgcagcc 1920 ggtatgggac ggcaaggcct tccagccgcg cctgatgctg ccgctgtcgc tgtcctacga 1980 tcaccgggtg atcaacggcg ccgccgcggc gcgcttcacc aagcgtctcg gcgacctgct 2040 ggcggatatc cgcgccatcc tgctgtgagc ttcccttaaa gagcgagccc gccacgctct 2100 ttaatcttcg gccccgccct tcgtggcggg gcttttttat gc 2142 // |
|