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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X12370; SV 1; linear; genomic DNA; STD; PRO; 1126 BP.
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AC   X12370;
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DT   24-FEB-1989 (Rel. 19, Created)
DT   23-OCT-2008 (Rel. 97, Last updated, Version 4)
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DE   Bacillus megaterium gdhA gene for glucose dehydrogenase A
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KW   gdhA gene; glucose dehydrogenase.
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OS   Bacillus megaterium
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
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RN   [1]
RP   1-1126
RX   DOI; 10.1111/j.1432-1033.1988.tb14124.x
RX   PUBMED; 3134196.
RA   Heilmann H.J., Maegert H.J., Gassen H.G.;
RT   "Identification and isolation of glucose dehydrogenase genes of Bacillus
RT   megaterium M1286 and their expression in Escherichia coli";
RL   Eur. J. Biochem. 174(3):485-490(1988).
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CC   Data kindly reviewed (16-Mar-1989) by Heilmann H.J.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1126
FT                   /organism="Bacillus megaterium"
FT                   /strain="M1286"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="lambda EMBL3."
FT                   /db_xref="taxon:1404"
FT   promoter        28..33
FT                   /note="-35 region"
FT   promoter        50..55
FT                   /note="-10 region"
FT   RBS             207..212
FT                   /note="pot. ribosome binding site"
FT   CDS             221..1006
FT                   /transl_table=11
FT                   /note="glucose dehydrogenase A (AA 1 - 261)"
FT                   /db_xref="GOA:P10528"
FT                   /db_xref="HSSP:1GEE"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P10528"
FT                   /protein_id="CAA30931.1"
FT                   /translation="MYTDLKDKVVVITGGSTGLGRAMAVRFGQEEAKVVINYYNNEEEA
FT                   LDAKKEVEEAGGQAIIVQGDVTKEEDVVNLVQTAIKEFGTLDVMINNAGVENPVPSHEL
FT                   SLDNWNKVIDTNLTGAFLGSREAIKYFVENDIKGNVINMSSVHEMIPWPLFVHYAASKG
FT                   GMKLMTETLALEYAPKGIRVNNIGPGAMNTPINAEKFADPEQRADVESMIPMGYIGKPE
FT                   EVAAVAAFLASSQASYVTGITLFADGGMTKYPSFQAGRG"
FT   terminator      1023..1067
FT                   /note="pot. stem-loop structure; pot. transcription
FT                   terminator;"
FT   repeat_region   1023..1041
FT                   /note="inverted repeat A"
FT   repeat_region   1049..1067
FT                   /note="inverted repeat A'"
XX
SQ   Sequence 1126 BP; 390 A; 188 C; 243 G; 305 T; 0 other;
     aagcttctca ttatcacaaa atgggaattg ttatttcaac gcttggcggt atcttcttat        60
     taggagaaaa gaaaacgaag cgtcagctta tttcgattac gattggtatt gtgctcatca       120
     ttttaggtgg cgtattcctc ggaatggcta aaagttaacg tttacgtaaa agttaatgat       180
     agtatgtatg aataaatgaa aacactagga ggattttatt atgtatacag atttaaaaga       240
     taaagtagtt gtaattacag gtggatcaac aggtttagga cgcgcaatgg ctgttcgttt       300
     cggtcaagaa gaagcaaaag ttgttattaa ctattacaac aatgaagaag aagctctaga       360
     tgcgaaaaaa gaagtagaag aagcaggcgg acaagcaatc atcgttcaag gcgatgtaac       420
     aaaagaagaa gacgttgtaa atcttgttca aacagctatt aaagaatttg gtacattaga       480
     cgtaatgatt aacaacgctg gtgttgaaaa cccagttcct tctcatgagc tatctctaga       540
     taactggaac aaagttattg atacaaactt aacaggtgca ttcttaggaa gccgtgaagc       600
     aattaaatac ttcgttgaaa acgacattaa aggaaatgtt atcaacatgt ctagcgttca       660
     cgaaatgatt ccttggccat tatttgttca ctacgcagca agtaaaggcg gtatgaaact       720
     aatgacggaa acattggctc ttgaatatgc gccaaaaggt attcgcgtaa ataatattgg       780
     accaggtgcg atgaacacac caattaacgc agagaaattt gcagatccag aacaacgtgc       840
     agacgtagaa agcatgattc caatgggtta catcggtaaa ccagaagaag tagcagcagt       900
     tgcagcattc ttagcttcat cacaagcaag ctatgtaaca ggtattacat tatttgcaga       960
     tggcggtatg acgaaatacc cttctttcca agcaggaaga ggctaatagt ttttaatcga      1020
     tgtagaaaaa agagacatgg caaaaagcgc catgtctctt ttttctatac ctaagcgaat      1080
     gacacccccc tttcggggtt ggtggcattc gcctaggtaa aagctt                     1126
//
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