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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X07689; SV 1; linear; genomic DNA; STD; PRO; 1735 BP.
XX
AC   X07689;
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DT   16-JAN-1989 (Rel. 18, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 3)
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DE   S. typhimurium LT2 metF gene for 5,10 methylenetetrahydrofolate reductase
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KW   5,10-methylenetetrahydrofolate reductase; metF gene; reductase.
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OS   Salmonella enterica subsp. enterica serovar Typhimurium
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Salmonella.
XX
RN   [1]
RP   1-1735
RX   DOI; 10.1007/BF00334692
RX   PUBMED; 2841568.
RA   Stauffer G.V., Stauffer L.T.;
RT   "Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF
RT   gene and its homology with the corresponding sequence of Escherichia coli";
RL   Mol. Gen. Genet. 212(2):246-251(1988).
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CC   Data kindly reviewed (23-Feb-1989) by Stauffer L.T.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1735
FT                   /organism="Salmonella enterica subsp. enterica serovar
FT                   Typhimurium"
FT                   /strain="JL781."
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="pACYC184"
FT                   /clone="pGS174"
FT                   /db_xref="taxon:90371"
FT   promoter        334..339
FT                   /note="-35 region"
FT   misc_feature    354..393
FT                   /note="pot. operator sequence"
FT   promoter        357..362
FT                   /note="-10-like region"
FT   misc_feature    366..366
FT                   /note="mRNA start site"
FT   RBS             425..428
FT                   /note="pot. ribosome binding site"
FT   CDS             435..1325
FT                   /transl_table=11
FT                   /note="metF protein (AA 1-296)"
FT                   /db_xref="GOA:P11003"
FT                   /db_xref="InterPro:IPR003171"
FT                   /db_xref="InterPro:IPR004620"
FT                   /db_xref="UniProtKB/Swiss-Prot:P11003"
FT                   /protein_id="CAA30531.1"
FT                   /translation="MSFFHANQREALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNSI
FT                   DRLSSLKPKFVSVTYGANSGERDRTHSVIKGIKERTGLEAAPHLTCIDPTRDELRTIAR
FT                   DYWNNGIRHIVALRGDLPPGSGKPEMYAADLVGLLKEVADFDISVAAYPEVHPEAKSAQ
FT                   ADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKK
FT                   FADMTNVRIPSWMSLMFEGLDNDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAE
FT                   MSYAICHTLGVRPGL"
FT   terminator      1331..1348
FT                   /note="put. transcription terminator"
XX
SQ   Sequence 1735 BP; 428 A; 429 C; 451 G; 427 T; 0 other;
     cgggttaccg tgctgacgct caatggcaag ccgattgacg atgctacggt ttatcacatc        60
     gctaccaaca gcttccttgc cgacggcggc gatggttttg cggcattcac ggaaggccag       120
     gcgcggaaca cctccggcgg ctactatgtg tcgaatgcga tagttgatta ctttaaggcg       180
     ggcaacacca tcacggatga gcagctcaaa ggaatgcgcg ttgcggatgt gaagaagtaa       240
     tttcccctgc cgcggcgcgt tcagtgtcgc ggcgtgacat gaagaaaatt caactttgct       300
     gaatattcct cattctttgg cgtgattttt cagttgacgt ccttctgctt ttccgtcatt       360
     tttacatctg gacgtctaaa cggatagatg tttacaacac aacaaataat aacatgcgat       420
     tgatgaggta aggtatgagc ttttttcacg ccaaccagcg ggaagccctg aatcagagcc       480
     tggcggaagt acagggtcag attaacgttt cgtttgaatt tttcccgccg cgcaccagtg       540
     aaatggagca aaccctgtgg aactccatcg atcgcctgag cagtctgaaa ccgaagtttg       600
     tttcggtaac gtatggcgcc aactccgggg aacgcgaccg cacccatagc gttattaaag       660
     gcatcaaaga gcgtactggg cttgaggccg cgccgcacct tacctgtatt gaccccacgc       720
     gcgatgaact gcgcaccatc gcccgcgact actggaataa cggtatccgc cacattgtcg       780
     ctttgcgcgg cgatttgccg ccgggcagcg gtaagccgga gatgtacgcc gccgatctgg       840
     ttggtttgct caaagaggtg gccgatttcg atatttcagt agcggcctat ccggaggtac       900
     atccggaagc gaaaagcgcg caggccgatc tgcttaatct gaagcgtaaa gtggatgctg       960
     gcgctaaccg cgcgataacc caatttttct tcgatgtgga aagctacctg cgttttcgcg      1020
     accgctgtgt ttctgccggt atcgacgtag aaattattcc cggcatttta ccggtgtcta      1080
     actttaaaca ggcaaaaaaa tttgccgata tgaccaatgt ccgcattccg tcctggatgt      1140
     cactgatgtt tgaggggctg gataatgacg cagaaacccg caagctggtg ggcgctaaca      1200
     ttgcgatgga catggtgaaa attttaagcc gtgaaggagt gaaggatttc cacttctaca      1260
     cgttgaatcg tgcggaaatg agttatgcca tttgccacac gctgggcgta agaccgggtt      1320
     tataacctcc agctccctgc cgggagcttt attacaactc atattgatct acatctctgt      1380
     aactaaaaat ataaaaggta ttagctatcg aatctgtgga ttaattcaac tatatctatt      1440
     tgctcctggt gtatatcgta acggtaacac tttaaaaggg agctgagata tgagcacgac      1500
     cgacgatacc cataacacgt tatccactgg aaaatgtcct ttccatcagg gggggcatga      1560
     ccgaagcgca ggcgcaggga ctgccagccg cgactggtgg ccgaaccagc ttcgcgtgga      1620
     tcttttgaat caacattcca accgttctaa cccgctgggt gaagactttg actaccgcaa      1680
     agagtttagc aagttagact actccgccct gaaaggggat ctcaaggcgc tgctg           1735
//
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