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EBI DbfetchID X06997; SV 1; linear; genomic DNA; STD; FUN; 7127 BP. XX AC X06997; XX DT 02-APR-1988 (Rel. 15, Created) DT 12-JUN-2006 (Rel. 88, Last updated, Version 5) XX DE Kluyveromyces lactis LAC12 gene for lactose permease XX KW lactose permease; unidentified reading frame. XX OS Kluyveromyces lactis OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces. XX RN [1] RP 1-7127 RA Chang Y.D.; RT ; RL Submitted (26-FEB-1988) to the EMBL/GenBank/DDBJ databases. RL Chang Y. D., Dept. of Biochemistry, University of Kentucky Medical Center, RL , Lexington, Kentucky, 40536-0084. XX RN [2] RX PUBMED; 3053697. RA Chang Y.D., Dickson R.C.; RT "Primary structure of the lactose permease gene from the yeast RT Kluyveromyces lactis. Presence of an unusual transcript structure"; RL J. Biol. Chem. 263(32):16696-16703(1988). XX DR TRANSFAC; R04130; KLULA$LAC12_01. DR TRANSFAC; R04131; KLULA$LAC12_02. XX CC the sequence submitted starts from the 5'end of LAC4 gene but goes CC to the opposite direction; therefore, base number 1 is -1199 of CC LAC4 gene; for LAC4 gene seq. see CC Mol. Cell. Biol. (1987)7,4369-4376 XX FH Key Location/Qualifiers FH FT source 1..7127 FT /organism="Kluyveromyces lactis" FT /strain="Y1140 (wild type)" FT /mol_type="genomic DNA" FT /clone_lib="Sau3A" FT /clone="pCKR1B-LAC4-1" FT /db_xref="taxon:28985" FT precursor_RNA <1496..1496 FT /note="transcript" FT precursor_RNA <1494..1494 FT /note="transcript" FT precursor_RNA <1489..1489 FT /note="transcript" FT precursor_RNA <1485..1485 FT /note="transcript" FT misc_feature 991..1007 FT /note="put. upstream activating sequence 2" FT CDS 1616..3379 FT /note="lactose permease (AA 1-587)" FT /db_xref="GOA:P07921" FT /db_xref="InterPro:IPR005829" FT /db_xref="UniProtKB/Swiss-Prot:P07921" FT /protein_id="CAA30053.1" FT /translation="MADHSSSSSSLQKKPINTIEHKDTLGNDRDHKEALNSDNDNTSGL FT KINGVPIEDAREEVLLPGYLSKQYYKLYGLCFITYLCATMQGYDGALMGSIYTEDAYLK FT YYHLDINSSSGTGLVFSIFNVGQICGAFFVPLMDWKGRKPAILIGCLGVVIGAIISSLT FT TTKSALIGGRWFVAFFATIANAAAPTYCAEVAPAHLRGKVAGLYNTLWSVGSIVAAFST FT YGTNKNFPNSSKAFKIPLYLQMMFPGLVCIFGWLIPESPRWLVGVGREEEAREFIIKYH FT LNGDRTHPLLDMEMAEIIESFHGTDLSNPLEMLDVRSLFRTRSDRYRAMLVILMAWFGQ FT FSGNNVCSYYLPTMLRNVGMKSVSLNVLMNGVYSIVTWISSICGAFFIDKIGRREGFLG FT SISGAALALTGLSICTARYEKTKKKSASNGALVFIYLFGGIFSFAFTPMQSMYSTEVST FT NLTRSKAQLLNFVVSGVAQFVNQFATPKAMKNIKYWFYVFYVFFDIFEFIVIYFFFVET FT KGRSLEELEVVFEAPNPRKASVDQAFLAQVRATLVQRNDVRVANAQNLKEQEPLKSDAD FT HVEKLSEAESV" FT CDS <3949..5217 FT /note="ORF II" FT /db_xref="GOA:P08540" FT /db_xref="InterPro:IPR007312" FT /db_xref="UniProtKB/Swiss-Prot:P08540" FT /protein_id="CAA30054.1" FT /translation="KMKFSDFSVLGLGALALNAVTVSANTADTALLRTYSTISPSLSEI FT ESAASATEVAEVVSDVEGAAFKRFFIIFLENTDYDKAAGDESLSWLAEQGITLTNYWAL FT THPSEPNYLASVGGDYFALDDDRFISMPSNVSNIVDLLDTKGISWAEYQEHSPYAGFQG FT MNFSNQETYASDYVRKHNPLILFDNVVNNDTRLANIKNFEDFNNDVENEKLPQYAFITP FT NMTNDGHDTTIQFAGKWSKDFLAPLLENDYFMEDTLVLLTFDENETYGIKNKVFSILLG FT GVIPDELKGTKDDTFYDHYSQLASVEANWDLPHLGRHDGDANVLEIVANATNITNVEVD FT TTYMINETYIGYLNDYNIELPAPNVTAINRNGQPILDSIKETWEDEYSKQVSESYYTST FT TTTVSADVTDAETFSNFYRYRQC" XX SQ Sequence 7127 BP; 1970 A; 1436 C; 1490 G; 2231 T; 0 other; gcgacttgga aggccaaagg actatcgcat gattattcgc cgggaacccg cgagttccct 60 gctcttttct ttcaaaccag gcagcaaacc aggtgaacac actctgatgt agtgcagtcc 120 ctaagtcctt tgaagattcg gggagctagc tacccacgcg aatgtaacaa aagaacattt 180 acttttgtgg ggggtggaaa agtcgattag gatcttgcag cacagaaact gcgcaggggt 240 ttttttcatc ttggagaagc aactggctaa attcgacaca aacaaaaact gaaaaatgga 300 aaataaaaaa tgaaaaagca agctgaattc gaagaaggga gaattccgcc ttctgcaacc 360 acactaatgg ttggtagtca atagatacgc attagaaggt tactatttta tgagtcgatc 420 cccgcggaaa tttaggaatt ttaaacttgg gcttgatata cgtcggtata gtgtatctgg 480 tatttttgcc aatggttcct gtgcccgtgc ccgtgcccgt gtgctcatgc gatgcattgt 540 gactactgct tggcaaggca aatcagcggt ctgcggccgt actctaaagc cggacagaca 600 tggtggggtg ccttttcggt ccataagtgt aactagctag ctacaagtat gttctgtgca 660 aagatggtaa aggttgcata cagtcaagtt gctgtccttg gacctggtct gacctgacct 720 agcttaacat tgcatagcgc atacccactt ggatggatgc aggagtgtta caaatcggcg 780 aacagttctc cttgtttgtt tttggtcata gtcacgtccg gctacacaca agatatggca 840 cagtatgtgt agatgcgagc gaagatacgg ctggaaaaca ggtgggtttt gaaggataga 900 cagaaggcaa gagggtgggc aaaaagtcgg gcactcaggc aagtattggc atgatcaaac 960 cagattaagt tctaggccaa gaacatattt cggcccactg actcccgcca aaaaaaaaga 1020 aaaaaaccta atttgacagt ctcggaattt tatctatctg ttcctttcct ttggttacta 1080 gtatcgtcat ttaatttttt ttggaattgg caagttacct gcgtggctct ataccgaatg 1140 aaccccgacc ctggccgttc ggtgcaccga gcgcgtttac gtgtgtacac gcttttgtaa 1200 gaaagaaact cgggtctgcg tgggcctgca acgtgactta gcaatacccg cgcgtcagat 1260 ttaacatatt cgggattctt ccctccgtac tagaaagttg acaataagat gtccatactg 1320 ttgttgatac cttgctactc tagctcatta tgacaaggac aatcggttaa ttggaggtct 1380 aaatggtgta tcaaggtata taaaggcagt aactttcaat gttgagttgg gctgtagttc 1440 aaggtggttg tagcttctgg aacatatata tgctattcaa tatagctgga aagtgtaagc 1500 taaacagcaa acaaaagtct ctgtgtgttg tcttataatt atatccttca gtcagtgtta 1560 atgattaaag acagaattac gaaaaaataa aacaagacga taatcagttt tcaaaatggc 1620 agatcattcg agcagctcat cttcgctgca gaagaagcca attaatacta tcgagcataa 1680 agacactttg ggcaatgatc gggatcacaa ggaagccttg aacagtgata atgataatac 1740 ttctggattg aaaatcaatg gtgtccccat cgaggacgct agagaggaag tgctcttacc 1800 aggttacttg tcgaagcaat attacaaatt gtacggttta tgttttataa catatctgtg 1860 tgctactatg caaggttatg atggggcttt aatgggttct atctataccg aagatgcata 1920 tttgaaatac taccatttgg atattaactc atcctctggt actggtctag tgttctctat 1980 tttcaacgtt ggtcaaattt gcggtgcatt ctttgttcct cttatggatt ggaaaggtag 2040 aaaacctgct attttaattg ggtgtctggg tgttgttatt ggtgctatta tttcgtcttt 2100 aacaacaaca aagagtgcat taattggtgg tagatggttc gtggcctttt tcgctacaat 2160 cgctaatgca gcagctccaa catactgtgc agaagtggct ccagctcact taagaggtaa 2220 ggttgcaggt ctttataaca ccctttggtc tgtcggttcc attgttgctg cctttagcac 2280 ttacggtacc aacaaaaact tccctaactc ctccaaggct tttaagattc cattatactt 2340 acaaatgatg ttcccaggtc ttgtgtgtat atttggttgg ttaatcccag aatctccaag 2400 atggttggtt ggtgttggcc gtgaggaaga agctcgtgaa ttcattatca aataccactt 2460 aaatggcgat agaactcatc cattattgga tatggagatg gcagaaataa tagaatcttt 2520 ccatggtaca gatttatcaa accctctaga aatgttagat gtaaggagct tattcagaac 2580 gagatcggat aggtacagag caatgttggt tatacttatg gcttggttcg gtcaattttc 2640 cggtaacaat gtgtgttcgt actatttgcc taccatgttg agaaatgttg gtatgaagag 2700 tgtctcattg aatgtgttaa tgaatggtgt ttattccatc gtcacttgga tttcttcaat 2760 ttgcggtgca ttctttattg ataagattgg tagaagggaa ggtttccttg gttctatctc 2820 aggtgctgca ttagcattga caggtctatc tatctgtact gctcgttatg agaagactaa 2880 gaagaagagt gcttccaatg gtgcattggt gttcatttat ctctttggtg gtatcttttc 2940 ttttgctttc actccaatgc aatccatgta ctcaacagaa gtgtctacaa acttgacgag 3000 atctaaggcc caactcctca actttgtggt ttctggtgtt gcccaatttg ttaatcaatt 3060 tgctactcca aaggcaatga agaatatcaa atattggttc tatgtgttct acgttttctt 3120 cgatattttc gaatttattg ttatctactt cttcttcgtt gaaactaagg gtagaagctt 3180 agaagaatta gaagttgtct ttgaagctcc aaacccaaga aaggcatccg ttgatcaagc 3240 attcttggct caagtcaggg caactttggt ccaacgaaat gacgttagag ttgcaaatgc 3300 tcaaaatttg aaagagcaag agcctctaaa gagcgatgct gatcatgtcg aaaagctttc 3360 agaggcagaa tctgtttaaa gccatctttt caatatattt tgttaggtgc aagaagtttc 3420 cgtacttcat aatttgtttt ttattctgct tgatcttctc ctaattgcag caaaaagtct 3480 tgtggaattc atcaattaaa aagccacagg ctttagactc ttaatggatt attctaacag 3540 ttactgttag acgttagcac atgggccggt atgtcattga agtacgttta aattgcctga 3600 attgggaaaa gtattgacct ttgagccggt tgcataccgc cttgcggtat gcagtttggc 3660 tcggttttcc cagcacgcat gtgggcatca tttccacacg tgtgaaccct cggcagttaa 3720 atgtgtgcat ttaggatcgg ctaatagttg tttttagctt cagcttcagc tcatcggatt 3780 ttgtgaaaca tataaatctg gcgtcaattg agtctctcga atggttgaaa ggtcaattca 3840 ggttggaatt ttgtattatg ttagctatat gggattgatc aaaaaaacca gccaaggatt 3900 cagagaatta cagcgcaagc tcaggtagca ctccagtttt aaacataaaa aatgaaattc 3960 tccgatttta gtgttctcgg attaggtgct cttgccttaa atgccgttac cgtcagtgct 4020 aacactgctg acaccgcttt gttgagaact tactctacta tctctccaag tttgagtgaa 4080 attgaatccg ccgcttctgc tactgaggtt gctgaggtgg tttcagatgt cgaaggtgcc 4140 gctttcaaga gattcttcat tatcttcttg gaaaacaccg attacgacaa ggctgctggt 4200 gatgaatccc tttcatggct ggctgaacaa ggtattacct tgaccaacta ctgggctttg 4260 actcaccctt cagaaccaaa ctatttggct tctgtcggtg gtgattactt cgccttggat 4320 gatgacagat ttatttctat gccatccaat gtgtctaaca tcgttgattt gttggacact 4380 aagggtatct cttgggctga ataccaagaa cactctccat atgctggttt ccaaggtatg 4440 aacttctcta accaagagac atacgccagt gattatgtca gaaaacataa cccattgatt 4500 ttgttcgata acgttgtcaa caacgatact cgtttggcta acattaagaa ctttgaagat 4560 ttcaacaatg acgtagaaaa cgaaaagtta cctcaatacg ctttcattac tccaaatatg 4620 actaacgatg gtcatgacac cactatccaa tttgctggta aatggtcaaa ggacttcttg 4680 gctccattat tagaaaacga ttacttcatg gaggacactt tggtcttgtt gaccttcgat 4740 gaaaatgaaa cctatggtat caagaacaag gtcttctcca tcttgttggg tggcgttatc 4800 ccagatgaat tgaagggtac caaggatgat actttctatg accattactc tcaattggct 4860 agtgttgaag ccaactggga tttgcctcat ttaggtagac acgatggtga cgccaatgtt 4920 ttggaaattg ttgctaacgc taccaacatc accaacgttg aagttgacac cacttacatg 4980 atcaacgaaa cctatattgg ttacttgaac gattacaaca ttgaattgcc agctccaaat 5040 gttactgcta ttaacagaaa cggtcaacca atcttggact ccatcaagga aacttgggaa 5100 gatgaatatt ctaagcaagt ctctgaatcc tactatactt ccaccactac taccgtttct 5160 gcagatgtta ctgatgctga aaccttctcc aatttctacc gctaccgaca gtgctgatga 5220 aactagtgct accgctacat cttcttccaa cagcacttct gctgaaactt taagctcttc 5280 cgccggtgct ccatcaatga gcacttctcc ggtaacgtct tgttgggtgt cattgctgcc 5340 tttttcttat gaaaagatat cgactcaaag gagtagcagc acactcctat agagaaacta 5400 gagtgaaaac ggcagttcac tcttagttaa tattatttat tccgtacaaa tagaacagtt 5460 gaaaagtgac gcttttataa aataagaact tgattttgat ttgacctttt aattattaac 5520 cttatcattg aataataagt attatttcct tttacttgat atcttcttag tcttgacaac 5580 tgaaatattg tacgtctgtc ttgaaattta gctagtggtt caagaaccgt cataggaaaa 5640 aatacctatt ttttgtggta tgctgttagt aatgaatata tgttttgatt gtatatctaa 5700 tcgagaaaaa gaaatgcaga atgacagaga ctcctgtctg gtttcaatgt catgaaaagt 5760 tgtgcataag caaacttaac aattagataa gatggaaaag ttcaagtact cccctcccca 5820 ttgttgattc tgaattgtaa taccttgtcc taatcggaat tttcatgaca agagggtcaa 5880 gcccaacttt cttttaatgc cctagtcact aatatctcat tttcattttc cgcagttaga 5940 ttggttagat gtggcagaat ttttggaacg aacctcccgt gggttgtcta atattaaata 6000 cagacacttt ataaccagta ttcttttttt ttccttatta ttctcattta tttctctcat 6060 tacttttgtc caggaatatc ttaactctgg catcaatcac tgaagcaaag ttccacgtcg 6120 gggttcggca gtaaacccat actgtctttc gtgccctcct tataaacttt tcacctaagt 6180 aaacattagt tcttagtatt ccaaatttcg tacaccagct atcattataa cgatcagtat 6240 actgcaaagc ttcaaaagtt gagagttgac gtgccttccc taacaaaacc catcccatat 6300 cctttttgtc caacatctca aactttattt cagttttgtc tagtttctgt gttatgcaga 6360 taagctgtgc atataaatgt tggaaataag ctaagctgtt acgttttgtt ctcagttgtt 6420 agcaagagca gtaaatcaaa aatgattgtt cttttggaac cacccaagta aaaaacatat 6480 ccacatctgt aagtgtccgc gataaaaccg aacccgaaag tgagattcct cccattatta 6540 cgcacgaagg agttattctc atcttgtcag tagccttatc cgtatccgct tttttattat 6600 taccgtgaca ttcactaagc tattttgcta gcccactcct aacacatagc gttttgagcg 6660 tcttgggtac ttactatgaa aatgacagtt aactactttt gactagtcac aggttttaga 6720 tcaaagaatc ttgggaaaaa gatcgtattt tatagttaat tttattgttt catatctatt 6780 attttgaggt gctttacgga ctcctcgacc gatgcccttg agagccttca acccagtcag 6840 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 6900 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt 6960 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct 7020 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat 7080 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcg 7127 // |
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