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EBI DbfetchID X05918; SV 1; linear; genomic DNA; STD; FUN; 4193 BP. XX AC X05918; XX DT 15-JUL-1988 (Rel. 16, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 3) XX DE Kluyveromyces fragilis beta-glucosidase gene XX KW beta-glucosidase. XX OS Kluyveromyces marxianus OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces. XX RN [1] RP 1-4193 RX DOI; 10.1007/BF00436876 RX PUBMED; 2835179. RA Raynal A., Gerbaud C., Francingues M.C., Guerineau M.; RT "Sequence and transcription of the beta-glucosidase gene of Kluyveromyces RT fragilis cloned in Saccharomyces cerevisiae"; RL Curr. Genet. 12(3):175-184(1987). XX FH Key Location/Qualifiers FH FT source 1..4193 FT /organism="Kluyveromyces marxianus" FT /strain="ATCC 12424" FT /mol_type="genomic DNA" FT /clone_lib="PHCG3" FT /db_xref="taxon:4911" FT repeat_region 268..276 FT /note="direct repeat 1" FT repeat_region 307..315 FT /note="direct repeat 1" FT promoter 316..320 FT /note="TATA-Box like sequence" FT promoter 342..355 FT /note="TATA-Box like sequence" FT repeat_region 400..408 FT /note="direct repeat 2" FT promoter 406..412 FT /note="TATA-Box like sequence" FT repeat_region 416..424 FT /note="direct repeat 2" FT misc_feature 459..459 FT /note="pot. transcription start site" FT misc_feature 482..483 FT /note="pot. alternate transcription start site" FT CDS 612..3149 FT /note="beta-glucosidase (AA 1 - 845)" FT /db_xref="GOA:P07337" FT /db_xref="InterPro:IPR019800" FT /db_xref="UniProtKB/Swiss-Prot:P07337" FT /protein_id="CAA29353.1" FT /translation="MSKFDVEQLLSELNQDEKISLLSAVDFWHTKKIERLGIPAVRVSD FT GPNGIRGTKFFDGVPSGCFPNGTGLASTFDRDLLETAGKLMAKESIAKNAAVILGPTTN FT MQRGPLGGRGFESFSEDPYLAGMATSSVVKGMQGEGIAATVKHFVCNDLEDQRFSSNSI FT VSERALREIYLEPFRLAVKHANPVCIMTAYNKVNGDHCSQSKKLLIDILRDEWKWDGML FT MSDWFGTYTTAAAIKNGLDIEFPGPTRWRTRALVSHSLNSREQITTEDVDDRVRQVLKM FT IKFVVDNLEKTGIVENGPESTSNNTKETSDLLREIAADSIVLLKNKNNYLTSKERRQYH FT VIGPNAKAKTSSGGGSASMNSYYVVSPYEGIVNKLGKEVDYTVGAYSHKSIGGLAESSL FT IDAAKPADAENAGLIAKFYSNPVEERSEDEEPFHVTKVNRSNVHLFDFKHEKVDPKNPY FT FFVTLTGQYVPQEDGDYIFSLQVYGSGLFYLNDELIIDQKHNQERGSFCFGAGTKERTK FT KLTLKKGQVYNVRVEYGSGPTSGLVGEFGAGGFQAGVIKAIDDDEEIRNAAELAAKHDK FT AVLIIGLNGEWETEGYDRENMDLPKRTNELVRAVLKANPNTVIVNQSGTPVEFPWLEEA FT NALVQAWYGGNELGNAIADVLYGDVVPNGKLSLSWPFKLQDNPAFLNFKTEFGRVVYGE FT DIFVGYRYYEKLQRKVAFPFGYGLSYTTFELDISDFKVTDDKIDISVDVKNTGDKFAGS FT EVVQVYFSALNSKVSRPVKELKGFEKVHLEPGEKKTVNIELELKDAISYFNEELGKWHV FT EAGEYLVSVGTSSDDILSVKEFKVEKDLYWKGL" FT repeat_region 3314..3322 FT /note="inverted repeat A" FT misc_feature 3442..3445 FT /note="yeast termination consensus sequence" FT misc_feature 3472..3474 FT /note="yeast termination consensus sequence" FT misc_feature 3498..3501 FT /note="yeast termination consensus sequence" FT misc_feature 3541..3543 FT /note="yeast termination consensus sequence" FT repeat_region 3642..3652 FT /note="inverted repeat B" FT repeat_region 3655..3666 FT /note="inverted repeat B'" FT repeat_region 3714..3722 FT /note="inverted repeat A'" XX SQ Sequence 4193 BP; 1338 A; 675 C; 941 G; 1239 T; 0 other; attgaataat ggggctgcaa aaattaaggg tgctaaaata tataccagta ctacctaaag 60 cagtatgcat tcccattatt acttggagga agggccttcg aaagcgtttg aaacaggagt 120 aggagtagga gtattacgga gaaatgggca ttgaatagag gtagcaaagg aaaagccgtg 180 acaatgcggc aatgtaccca acctatgagt ctgtgtgtgt aaagtgtgtg tgtctttttt 240 tttttaattt taattttttt ttttaattat tgaaaaacca ggactacgtc aggttaggag 300 aatggatatt gaaaatatta atgtaccagt agttttgcat gtataattat tattaccaat 360 agacgaagtg gaggtattag agcgatttgg gggaattgtg attgtatata tagtgtgtat 420 gttttacggt actgaaagat cgtgttgtaa ggaaaaataa ggcacaggaa aaattcagat 480 tttttaaggg tttattacta ggagcagtag tagtagtaga gtagtggtag caaaagccga 540 gatctaggta gagacagatc tgtaatacat agctagttga agttgaagaa gtgcaaacgt 600 taagaaaaaa aatgtctaaa tttgatgttg aacagttatt gagtgaattg aaccaggatg 660 aaaagatttc cttactttct gcagttgatt tctggcatac taagaagatt gaacggttgg 720 gaattccagc ggtgagggtt tctgatggtc caaatggtat tagagggacg aagttctttg 780 atggggttcc ttcaggatgt ttccctaatg gtaccgggtt ggcatctact tttgatcgcg 840 atctgcttga gacagcaggt aagttgatgg ctaaggaatc gattgcgaag aatgctgctg 900 tgattttggg tccaaccaca aacatgcaac gtggtccttt gggtggtcgt ggttttgaat 960 cattctctga agatccatat cttgctggta tggctacttc ttctgttgtt aaaggtatgc 1020 aaggcgaagg tattgctgct accgttaagc attttgtttg taacgacttg gaagaccaac 1080 gtttttcttc gaactcaatt gtttctgaaa gggctctgag agaaatttac ttggagccct 1140 tcagattggc agttaaacat gccaatcctg tttgtataat gactgcttat aacaaggtca 1200 atggcgatca ttgctcccaa tccaagaagc tattgatcga cattttgaga gacgagtgga 1260 aatgggacgg tatgttaatg tccgactggt tcggtacata tacgactgcc gcagctatca 1320 agaatgggtt ggatatcgag ttccctggac caacaagatg gagaacacgt gctttagtgt 1380 ctcactctct caactccaga gaacaaatca ctactgaaga tgttgatgat cgtgttagac 1440 aagtgctaaa aatgattaag ttcgttgttg acaatttaga gaaaacaggt attgtggaga 1500 atggcccaga atctacttca aacaacacca aggaaacctc ggacctgttg agagagattg 1560 ctgctgactc tattgtttta ttgaagaaca aaaacaatta tcttacctct aaagaaagaa 1620 gacaatatca tgtcattggc ccaaatgcta aagcaaagac tagttccggt ggtggttcag 1680 catctatgaa ctcctactat gttgtttctc cgtatgaagg tatcgtcaat aagctgggca 1740 aagaggtcga ttacaccgta ggggcctatt cacacaaatc gattggaggt ttggcagaga 1800 gtagtttgat cgatgctgca aaaccagcag atgctgaaaa tgctggatta attgccaagt 1860 tttactccaa tccagtagaa gagagatctg aagatgaaga accattccac gttaccaaag 1920 tcaatagatc caatgttcac ttatttgatt tcaaacatga gaaagtggat ccaaagaacc 1980 cttacttttt tgtaacctta accggacagt acgtgcctca agaagatggt gattatatct 2040 tcagtcttca agtttatggt tctggtttgt tctacttaaa cgatgagttg attattgacc 2100 aaaagcacaa ccaagaaagg ggtagtttct gctttggagc tggtaccaaa gaaagaacca 2160 aaaagttgac tttgaagaag ggccaagttt ataatgtaag agttgagtac ggttctggcc 2220 caacttcagg tttggttggg gaattcggtg caggtggatt ccaagctggt gtcattaagg 2280 cgatcgatga tgacgaggag attagaaacg cagcagaatt agcagctaag catgataagg 2340 ctgtgttgat aattggatta aatggtgaat gggaaaccga aggttatgac agagaaaaca 2400 tggatttgcc aaaaagaaca aatgaattag ttcgtgctgt tttgaaagca aatccaaata 2460 ctgttatcgt taaccaatca ggtaccccag ttgagttccc ttggttagaa gaggcaaatg 2520 cgctagttca agcttggtac ggtggtaatg aattgggtaa tgctatcgca gatgtcttgt 2580 acggtgacgt ggttccaaat ggtaagttat cgctctcttg gccatttaag ttgcaagata 2640 atccagcctt tttaaacttc aagaccgagt tcggaagagt tgtttacggt gaggatatct 2700 ttgttggtta taggtactac gaaaagcttc aaagaaaggt agccttcccc ttcggatatg 2760 gtctatcgta tacaacattc gaactagata tttctgactt caaggtaact gatgataaga 2820 tagatatttc agttgatgtg aagaatactg gtgataaatt tgctggctcc gaggtggtgc 2880 aagtctactt cagcgctcta aactctaagg tctcgagacc ggttaaggag ttgaagggat 2940 tcgaaaaagt tcatttggaa ccaggtgaga agaagacagt taatattgaa ctagaattga 3000 aagatgcaat ttcctacttt aacgaagagc tcggtaaatg gcacgttgaa gcaggtgaat 3060 acttggtttc agttggtact tcttctgatg atatactttc cgtcaaagag tttaaagtag 3120 aaaaagactt gtactggaaa ggtttgtgaa gaatgctaaa atggtttagt gtttccaatc 3180 caggtgcaag tttcattgta cagttataat tatatatatg tgtaacgtgc aatgacccat 3240 cataacagag agttattcgc tattaaacac aaaaaacaaa caaaccagta actacatgaa 3300 atgaataggt attaaagtct tgaatttccc atgaaatacg aacctttaca gtttgaactt 3360 aacaatatgg cctttttaag ccatatcaac ctcatgaaat tacggggaag gaaacgatga 3420 aaggtcaaag cctattaagc atagtactgc atctaaggga gagtggtacc ctttacaagg 3480 ttttttgttt tccagagtag tttgcgaata ctacaaatac gttgaatttt tgaagttaca 3540 tttcattacg taacatttaa actaattaga tagtaaataa taaacatcgc aatacacatt 3600 aaatcattga attaacctat acagcttaga ttcgcagaat atattctaac agtaactgtt 3660 agaataatcc attaagagtc taaagcctgt ggctttttaa ttgatgaatt ccacaagact 3720 ttttgctgca attaggagaa gatcaagcag aataaaaaac aaattatgaa gtacggaaac 3780 ttcttgcacc taacaaaata tattgaaaag atggctttaa acagattctg cctctgaaag 3840 cttttcgaca tgatcagcat cgctctttag aggctcttgc tctttcaaat tttgagcatt 3900 tgcaactcta acgtcatttc gttggaccaa agttgccctg actgagccaa gaatgcttga 3960 tcaacgatcc tttcttgggt ttggagcttc aaagacaact tctaattctt ctaagcttct 4020 acccttagtt tcaacgaaga agaagtagat aacaataaat tcgaaaatat cgaagaaaac 4080 gtagaacaca tagaaccaat atttgatatt cttcatgcct ttggagtagc aaattgatta 4140 acaaattggg caacaccaga aaccaacatg ttgaggagtt gggccttaga tct 4193 // |
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