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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X05050; SV 1; linear; genomic DNA; STD; PRO; 1933 BP.
XX
AC   X05050;
XX
DT   07-JUN-1987 (Rel. 12, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   E. coli gene for EcoRII modification enzyme (EcoRII cytosine methylase)
XX
KW   cytosine methylase; EcoRII methylase; restriction enzyme.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-1933
RX   DOI; 10.1093/nar/15.1.313
RX   PUBMED; 3029675.
RA   Som S., Bhagwat A.S., Friedman S.;
RT   "Nucleotide sequence and expression of the gene encoding the EcoRII
RT   modification enzyme";
RL   Nucleic Acids Res. 15(1):313-332(1987).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1933
FT                   /organism="Escherichia coli"
FT                   /strain="HB101"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   promoter        151..156
FT                   /note="put. -35 region"
FT   promoter        174..178
FT                   /note="put. -10 region"
FT   misc_feature    191..192
FT                   /note="transcription start site"
FT   RBS             199..203
FT                   /note="put. rRNA binding site"
FT   CDS             208..1641
FT                   /transl_table=11
FT                   /note="EcoRII cytosine methylase (AA 1-477)"
FT                   /db_xref="GOA:P05101"
FT                   /db_xref="InterPro:IPR001525"
FT                   /db_xref="InterPro:IPR018117"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05101"
FT                   /protein_id="CAA28725.1"
FT                   /translation="MSEFELLAQDLLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAEL
FT                   LRKVGKNEWSRETLNRWINGKCSPKTLTLAEEELLRKMLPEAPAHHPDYAFRFIDLFAG
FT                   IGGIRKGFETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPE
FT                   NDAYAYINEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARII
FT                   RAKKPAIFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFL
FT                   PQHRERIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEY
FT                   LYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFA
FT                   NEENQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKL
FT                   LEPYILKAVNADSCKVERI"
FT   terminator      1648..1669
FT                   /note="stem-loop structure"
XX
SQ   Sequence 1933 BP; 525 A; 467 C; 475 G; 466 T; 0 other;
     gctttccctt caggcggggt tcatacagcg gccagccatc cgtcatccat accacgacct        60
     caaaggccga cagcaggccc agaagacgct ccagcgtggc caacgtgcgt tacctaacaa       120
     taaacctgtt taaatatcca gataaaaaca ttcaatctgg gtcaaatgag tgatacagtt       180
     tcacccataa gacccaatgg aggcaatatg tctgaatttg aattactggc gcaggatctg       240
     cttgagaaag cagaagcgga agaacaactg cgacaggaaa atgataaaaa gctgctcggg       300
     caggtgctgg aaatctatga ccagaagtac gtggctgaac tgcttagaaa agttggtaaa       360
     aatgagtgga gtcgcgagac tcttaatcgc tggattaatg gtaagtgctc acctaagacg       420
     ctgacgttag ccgaagagga acttctacga aaaatgcttc cggaagcgcc tgcacatcac       480
     cctgactatg ccttccggtt tattgacctg tttgctggga ttggaggtat acggaagggc       540
     ttcgaaacca tcggtggcca gtgcgttttt accagtgaat ggaataaaga ggctgtgcgc       600
     acatataaag ctaactggtt taacgatgct caggaacaca ctttcaatct cgatattcgt       660
     gaagtcacgc tcagtgataa acctgaagta cctgaaaacg atgcctatgc ttacattaat       720
     gagcatgtgc cggatcatga tgtacttcta gcaggtttcc cctgtcaacc gtttagcctt       780
     gcgggcgtaa gcaagaaaaa ctcgctcggg cgcgcgcatg gtttcgaatg tgaggctcag       840
     ggaacgcttt tcttcgatgt ggcgcgtatt atccgcgcaa aaaaacctgc catctttgtt       900
     cttgaaaacg ttaaaaacct gaagagccat gacaagggta aaacctttaa agtcatcatg       960
     gataccctcg acgaactggg ctatgaagtt gcggatgcag ctgagatggg caaaaacgat      1020
     cctaaagtta tcgacggaaa gcacttttta cctcagcacc gagaacgtat cgttttggtc      1080
     ggtttccgcc gtgatctgaa cattcaccag ggctttaccc tgcgcgatat tagtcgtttt      1140
     tatccggaac agcgtccgtc atttggcgaa ctgctggaac ccgtggttga cagcaaatat      1200
     atactgacgc cgaaactctg ggagtatctc tataactacg ccaaaaagca cgcagctaag      1260
     ggtaacggat tcggttttgg cctcgttaat cctgaaaata aagaaagcat tgcccgtacg      1320
     ctttctgctc gctatcacaa agacgggtct gaaattctga tagaccgtgg ctgggatatg      1380
     gccacaggtg aaacagactt cgcgaacgaa gaaaatcagg cgcatcggcc ccgcaggctg      1440
     actccgcgag agtgcgcgcg ccttatgggt tttgaaaaag tagatggcag gccttttcgc      1500
     attcctgtgt cagacactca gtcgtacagg cagttcggta actccgtagt ggtgcccgtg      1560
     tttgaagccg tagccaaact gcttgaacct tatatcctga aagcggttaa tgccgattcg      1620
     tgcaaggttg aacgaatctg atcgctcctc ccggtattta tgccgggaga taatctatgg      1680
     aatatctgcg taaagccctg tcagctcagc aataaacgca cctagcgtca ttagctcagc      1740
     tctcaccgcc tccgggtatt ttttgtgcag agatgatggc acgaccaatc tgacacccga      1800
     ctcccgcatc tcccgatatt gagccagaga aactccctcc tggagtgtaa acagatgcac      1860
     ctgatgaatt ttatcggcct cattcagtat ctgacgccag cgatccttac aggtagtctt      1920
     gactgccagc atg                                                         1933
//
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