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EBI DbfetchID X05031; SV 1; linear; genomic DNA; STD; PHG; 3526 BP. XX AC X05031; XX DT 19-SEP-1987 (Rel. 13, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 14) XX DE Bacteriophage T3 gene region 1-2.5 with primary origin of replication XX KW origin of replication; unidentified reading frame. XX OS Enterobacteria phage T3 OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; OC Autographivirinae; T7-like viruses. XX RN [1] RP 1-3526 RX DOI; 10.1016/0022-2836(87)90261-0 RX PUBMED; 3586029. RA Schmitt M.P., Beck P.J., Kearney C.A., Spence J.L., DiGiovanni D., RA Condreay J.P., Molineux I.J.; RT "Sequence of a conditionally essential region of bacteriophage T3, RT including the primary origin of DNA replication"; RL J. Mol. Biol. 193(3):479-495(1987). XX FH Key Location/Qualifiers FH FT source 1..3526 FT /organism="Enterobacteria phage T3" FT /strain="T3(+)-L" FT /mol_type="genomic DNA" FT /db_xref="taxon:10759" FT CDS <1..93 FT /codon_start=1 FT /transl_table=11 FT /note="gp 1" FT /db_xref="GOA:P07659" FT /db_xref="HSSP:1ARO" FT /db_xref="InterPro:IPR002092" FT /db_xref="UniProtKB/Swiss-Prot:P07659" FT /protein_id="CAA28696.1" FT /translation="HETQLDKMPPLPKKGNLNLQDILKSDFAFA" FT promoter 105..120 FT /note="promoter of gene 1.05" FT repeat_region 122..128 FT /note="inverted repeat A" FT misc_feature 122 FT /note="transcription initiation site (gene 1.05)" FT repeat_region 137..143 FT /note="inverted repeat A'" FT rep_origin 145..221 FT /note="primary origin of replication" FT misc_feature 151..155 FT /note="primase binding site" FT RBS 165..170 FT /note="pot.ribosome binding site" FT CDS 180..452 FT /transl_table=11 FT /note="gp 1.05 (AA 1-90)" FT /db_xref="UniProtKB/Swiss-Prot:P07715" FT /protein_id="CAA28697.1" FT /translation="MINIKTFFKNIFKLNRLTSIKFYAWMPGSDDLRKTEFKLGLGPCG FT KVVTKLECYSTSSGMVIFQTTEDNEMKSFYYPKGSTCGRIERTYS" FT promoter 444..462 FT /note="promoter of gene 1.1" FT misc_feature 464 FT /note="transcription initiation site (gene 1.1)" FT misc_feature 466..516 FT /note="pot. stem-loop structure; RNAase III recognition FT site" FT repeat_region 466..487 FT /note="inverted repeat B" FT repeat_region 494..516 FT /note="inverted repeat B'" FT RBS 532..536 FT /note="pot. ribosome binding site" FT CDS 545..685 FT /transl_table=11 FT /note="gp 1.1 (AA 1-46)" FT /db_xref="InterPro:IPR013232" FT /db_xref="UniProtKB/Swiss-Prot:P20833" FT /protein_id="CAA28698.1" FT /translation="MRTNFEKFTKRDSVVNEQGEQWQERRDRMKKRHKQQRGNSQKREW FT N" FT RBS 672..676 FT /note="pot. ribosome binding site (gene 1.2)" FT CDS 685..963 FT /transl_table=11 FT /note="gp 1.2 (AA 1-92)" FT /db_xref="GOA:P07716" FT /db_xref="InterPro:IPR020147" FT /db_xref="UniProtKB/Swiss-Prot:P07716" FT /protein_id="CAA28699.1" FT /translation="MMMGRIYSGNLNDYKDAVARLQEDHDVTVKMESFSYENPAKMCRS FT CGEVLSVFTRSGHLVASRTFEHSDSDVQINAQTAWLRKVHSELKHWK" FT promoter 958..976 FT /note="promoter of gene 1.3" FT misc_feature 978 FT /note="transcription initiation site (gene 1.3)" FT misc_feature 982..1021 FT /note="pot. stem-loop structure; RNase III recognition FT site" FT repeat_region 982..998 FT /note="inverted repeat C" FT repeat_region 1005..1021 FT /note="inverted repeat C'" FT RBS 1045..1048 FT /note="pot. ribosome binding site (gene 1.3)" FT CDS 1058..2098 FT /transl_table=11 FT /note="gp 1.3 (AA 1-346)" FT /db_xref="GOA:P07717" FT /db_xref="HSSP:1A0I" FT /db_xref="InterPro:IPR016059" FT /db_xref="UniProtKB/Swiss-Prot:P07717" FT /protein_id="CAA28700.1" FT /translation="MNIFNTNPFKAVSFVESAVKKALETSGYLIADCKYDGVRGNIVVD FT NVAEAAWLSRVSKFIPALEHLNGFDKRWQQLLNDDRCIFPDGFMLDGELMVKGVDFNTG FT SGLLRTKWVKRDNMGFHLTNVPTKLTPKGREVIDGKFEFHLDPKRLSVRLYAVMPIHIA FT ESGEDYDVQNLLMPYHVEAMRSLLVEYFPEIEWLIAETYEVYDMDSLTELYEEKRAEGH FT EGLIVKDPQGIYKRGKKSGWWKLKPECEADGIIQGVNWGTEGLANEGKVIGFSVLLETG FT RLVDANNISRALMDEFTSNVKAHGEDFYNGWACQVNYMEATPDGSLRHPSFEKFRGTED FT NPQEKM" FT terminator 2109..2130 FT /note="pot. stem-loop structure; pot. transcription FT terminator" FT repeat_region 2109..2116 FT /note="inverted repeat D" FT repeat_region 2123..2130 FT /note="inverted repeat D'" FT promoter 2143..2161 FT /note="promoter gene 1.5" FT RBS 2163..2167 FT /note="pot. ribosome binding site (gene 1.5)" FT misc_feature 2163 FT /note="transcription initiation site (gene 1.5)" FT CDS 2177..2254 FT /transl_table=11 FT /note="gp 1.5 (AA 1-25)" FT /db_xref="UniProtKB/Swiss-Prot:P20835" FT /protein_id="CAA28701.1" FT /translation="MWLILFAIVATLGLMVADDNIWPDC" FT RBS 2252..2258 FT /note="pot. ribosome binding site (gene 1.6)" FT CDS 2267..2524 FT /transl_table=11 FT /note="gp 1.6 (AA 1-85)" FT /db_xref="InterPro:IPR018015" FT /db_xref="UniProtKB/Swiss-Prot:P07718" FT /protein_id="CAA28702.1" FT /translation="MRLHFNTSNGIFSVRREDRSTAVASERNAKLPLIGSVVPLSPRVH FT LLITRGEFIKAMNKERPHLEAVVTYWPRIRLFVKLIKEVL" FT RBS 2509..2518 FT /note="pot. ribosome binding site (gene 1.9)" FT CDS 2524..3015 FT /transl_table=11 FT /note="gp 1.9 (AA 1-163)" FT /db_xref="UniProtKB/Swiss-Prot:P07719" FT /protein_id="CAA28703.1" FT /translation="MLQHHWNKPDLEARFPVNSVVRYSGGGLKRILGMSGVVTGHSHTG FT LVKVRFGTQYAEVLPNNLIPLPKADVKTPNVEAPKTEVKSDVTHPNHYMLFDNVEAIEV FT IARSMTVEAFRGYCLGNILKYRLRAGKKSELATMDKDLKKAAFYQELFDKHRGLCYDAS FT " FT RBS 2990..2994 FT /note="pot. ribosome binding site (gene 1.95)" FT CDS 3002..3133 FT /transl_table=11 FT /note="gp 1.95 (AA 1-43)" FT /db_xref="UniProtKB/Swiss-Prot:P20312" FT /protein_id="CAA28704.1" FT /translation="MTLRECCDWCAAKWNQAIEDGDKAAADAYQQLYALWERRFKEA" FT RBS 3121..3128 FT /note="pot. ribosome binding site (gene 2.0)" FT CDS 3135..3299 FT /transl_table=11 FT /note="gp 2.0 (AA 1-54)" FT /db_xref="UniProtKB/Swiss-Prot:P20836" FT /protein_id="CAA28705.1" FT /translation="MEQREIKYLLTMEGNTESFEVPVFARSLEEATLQAEQYEDAGFVV FT TRIRPEVKA" FT promoter 3294..3312 FT /note="promoter (gene 2.5)" FT misc_feature 3314 FT /note="transcription initiation site (gene 2.5)" FT RBS 3338..3343 FT /note="pot. ribosome binding site (gene 2.5)" FT CDS 3351..>3526 FT /transl_table=11 FT /note="gp 2.5 (AA 1-59) (3526 is 2nd base in codon)" FT /db_xref="GOA:P20313" FT /db_xref="HSSP:1JE5" FT /db_xref="InterPro:IPR012340" FT /db_xref="UniProtKB/Swiss-Prot:P20313" FT /protein_id="CAA28706.1" FT /translation="MAGFKKKVYTSGLGTAEPYAYLSKPDYGNEERGFGNPRGVYKVDL FT TLSNKDPRCQAMV" XX SQ Sequence 3526 BP; 941 A; 866 C; 906 G; 813 T; 0 other; cacgagaccc aactggacaa gatgcctccg cttccgaaga aaggaaacct gaacctgcaa 60 gacattctca agtctgactt tgcctttgca taacaagcac ttagcattaa ccctcactaa 120 cgggagacta cttaaggtct cccactttaa gacactttag gtactaagag attaaattta 180 tgattaacat taagactttc tttaagaaca tctttaagtt aaaccgtttg acttccatta 240 agttctacgc ttggatgccc ggtagtgatg acctccgcaa gactgaattt aagttaggct 300 tagggccgtg cggtaaggtg gtcacgaagt tggaatgtta ctcaacgtcc tccgggatgg 360 tcatcttcca gaccactgag gacaacgaaa tgaaatcctt ctactacccg aaaggttcaa 420 cgtgtggtcg tatcgaacgg acatacagtt aaccctcact aacgggagag ttaaacttaa 480 ggtcatcacc aacggtggcc tttgtgatta actttcaata cacatcaaca tgaggtaaga 540 tactatgcgt accaactttg agaaatttac caagcgcgat tcagtggtca acgagcaggg 600 cgaacagtgg caagagcgcc gtgaccgcat gaagaaacgc cacaagcaac agcgcggtaa 660 ctcacagaaa cgggagtgga actgatgatg atgggacgta tttatagcgg caacctgaac 720 gattacaaag atgcggtagc gcgtctacag gaagaccatg atgtgaccgt gaagatggag 780 tcattcagct acgaaaaccc agcgaagatg tgcaggtcat gcggtgaggt tctcagtgtg 840 ttcacacgct ccgggcatct ggtggcatcc agaaccttcg agcatagcga cagcgatgta 900 caaatcaacg cgcagactgc atggctccgt aaggttcaca gcgaattgaa acactggaag 960 taataaccct cactaacagg agaatcctta aggtcacttt aacgagtggc cttcgtgatt 1020 cgaactgatt aactttcaaa caatgaggaa caaccgtatg aacatcttca ataccaaccc 1080 attcaaagct gtgtcattcg tagagtctgc cgttaagaaa gcgctggaga cctccggcta 1140 tcttatagcg gattgtaaat atgatggcgt gcgcggtaac attgtggttg ataacgtggc 1200 tgaggctgcg tggctgtccc gtgtgtccaa gtttatcccg gcgctggaac acctgaacgg 1260 tttcgataag cgctggcaac aactgctgaa cgatgaccga tgcatcttcc cggatggctt 1320 catgctggat ggcgaactga tggtcaaagg tgtagacttc aacaccgggt ctggcctgct 1380 gcgtaccaag tgggtgaagc gtgacaacat ggggttccac cttacgaacg tcccaactaa 1440 gctgaccccg aaaggccgcg aagttatcga cggtaagttt gagttccacc ttgacccgaa 1500 gcgcctgagc gtgcgactct acgctgtcat gccgattcac attgcggagt ctggcgagga 1560 ctacgatgta cagaacctgc tgatgccgta tcatgtggaa gccatgcgct ctcttctggt 1620 tgaatacttc ccggaaatcg agtggcttat cgctgagacc tacgaggtct acgatatgga 1680 ttcgctgact gaactgtacg aagagaagcg tgccgaaggt cacgagggtc tcattgtgaa 1740 agacccgcaa ggcatctaca agcgaggcaa gaagtctggc tggtggaagc tcaagcctga 1800 gtgtgaagct gacggtatca ttcagggtgt caattgggga accgaagggt tagccaacga 1860 gggtaaagtg ataggcttta gtgtgcttct tgagactggt cgtttagtag acgccaacaa 1920 catctctcgc gcattgatgg acgagttcac atccaacgtt aaagcccacg gtgaagactt 1980 ctacaatggt tgggcctgtc aggtcaacta catggaagcg accccggacg gctccctgcg 2040 acaccctagc ttcgagaagt tccgaggaac tgaggacaac cctcaagaga aaatgtaacc 2100 aactcactgg ctcaccttca cgggtgggcc tttcttcgtt ccgggcatta accctcacta 2160 acaggagaca cacaccatgt ggcttatcct attcgctatc gtcgcaacgc tgggattaat 2220 ggttgccgac gacaacattt ggcctgattg ttaaggagac aacatcatgc gtttacactt 2280 caacacatca aatggtatct tttcggttcg ccgtgaagac cgctctacag cagtggcctc 2340 tgagcgcaac gccaagctgc cgctgattgg ttcggtcgtc ccattgtcac cacgtgttca 2400 cctactgatc actcgtggag agttcattaa ggcgatgaac aaagagcgcc cacatctgga 2460 agccgtggtc acttactggc ctcgcatccg tctgttcgtt aagttgatta aggaggtgct 2520 gtaatgttac aacatcattg gaacaaacca gacttagagg ctcgcttccc ggttaactct 2580 gttgtgcgat actccggcgg tggcctcaag cgcatcttag ggatgagcgg agtggtcact 2640 ggtcactcac acacgggtct agttaaggtt cgcttcggta ctcaatacgc tgaggttctt 2700 ccgaacaacc tgattccgct gcctaaagct gacgttaaga ctccgaacgt ggaagcaccg 2760 aagacagaag tcaagtctga cgtaacccac cctaaccact acatgctgtt cgacaacgtg 2820 gaagccattg aggttatcgc ccggtcaatg acagtcgagg cgttccgtgg gtactgctta 2880 gggaacatcc tcaagtatcg cctgcgtgct ggcaagaagt ccgaactggc aacgatggat 2940 aaagacctca agaaagctgc attctatcag gaactctttg acaaacaccg ggggctgtgt 3000 tatgacgctt cgtgaatgct gcgattggtg tgccgctaag tggaaccaag ctattgagga 3060 tggtgacaag gcggctgctg acgcttacca acaactgtac gcgctgtggg agcggcgctt 3120 taaggaggct taacgtggag caacgtgaaa ttaaatacct tctgacgatg gaaggtaaca 3180 ccgagtcctt tgaggttccg gtattcgccc ggtctctcga agaggcaact ctacaggctg 3240 aacaatatga ggacgctggg ttcgtagtga ctcgcatccg tccagaagtg aaagcctaat 3300 taccctcact aaagggaaca acccaaccta tcaaccaaag gagaaacaat atggctggtt 3360 tcaagaagaa agtttacacc tccggtctcg gtactgctga accttatgct tacctgagca 3420 agccggacta tggtaacgaa gagcgtggct tcggcaaccc tcgtggtgtc tacaaggtag 3480 acctgactct ttccaacaaa gacccgcgct gtcaggcaat ggtcga 3526 // |
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