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EBI DbfetchID X04603; SV 1; linear; genomic DNA; STD; PRO; 2400 BP. XX AC X04603; XX DT 07-JUN-1987 (Rel. 12, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 8) XX DE B. subtilis thrB and thrC genes for homoserine kinase and threonine DE synthase (EC 2.7.1.39 and EC 4.2.99.2, respectively) XX KW homoserine kinase; kinase; overlapping genes; thrB gene; ThrC gene; KW threonin synthase; unidentified reading frame. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2400 RX PUBMED; 3098560. RA Parsot C.; RT "Evolution of biosynthetic pathways: a common ancestor for threonine RT synthase, threonine dehydratase and D-serine dehydratase"; RL EMBO J. 5(11):3013-3019(1986). XX FH Key Location/Qualifiers FH FT source 1..2400 FT /organism="Bacillus subtilis" FT /strain="168" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS <1..248 FT /codon_start=3 FT /transl_table=11 FT /product="unknown protein" FT /note="ORFX" FT /db_xref="GOA:P19582" FT /db_xref="InterPro:IPR001342" FT /db_xref="InterPro:IPR002912" FT /db_xref="InterPro:IPR005106" FT /db_xref="InterPro:IPR016040" FT /db_xref="InterPro:IPR016204" FT /db_xref="InterPro:IPR019811" FT /db_xref="UniProtKB/Swiss-Prot:P19582" FT /protein_id="CAA28269.1" FT /translation="IHVKDEVGSFSKITSVFSERGVTFEKILQLPIKGHDELAEIVIVT FT HHTSEADFSDILQNLNDLEVVQEVKSTYRVEGNGWS" FT CDS 248..1306 FT /transl_table=11 FT /gene="thrC" FT /product="threonine synthase" FT /EC_number="4.2.99.2" FT /db_xref="GOA:P04990" FT /db_xref="InterPro:IPR000634" FT /db_xref="InterPro:IPR001926" FT /db_xref="InterPro:IPR004450" FT /db_xref="UniProtKB/Swiss-Prot:P04990" FT /protein_id="CAA28270.1" FT /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL FT HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI FT IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK FT TAAFEVCEQLGEAPDVLAIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAI FT VRNEVIENPETIATAIRIGNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGV FT FAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLTGNGLKDPNTAVDISEIKPVTLPTDED FT SILEYVKGAARV" FT RBS 1286..1292 FT /gene="thrB" FT CDS 1303..2229 FT /transl_table=11 FT /gene="thrB" FT /product="homoserine kinase" FT /EC_number="2.7.1.39" FT /db_xref="GOA:P04948" FT /db_xref="InterPro:IPR000870" FT /db_xref="InterPro:IPR006203" FT /db_xref="InterPro:IPR006204" FT /db_xref="InterPro:IPR013750" FT /db_xref="InterPro:IPR014721" FT /db_xref="InterPro:IPR020568" FT /db_xref="UniProtKB/Swiss-Prot:P04948" FT /protein_id="CAA28271.1" FT /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS FT FEAETETVAGIRGTDNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVA FT AIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRVPNAD FT IDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKD FT MFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFP FT HCEVDALTVPKEGSIIERNPLYQVKSV" FT CDS 2233..>2400 FT /transl_table=11 FT /product="unknown protein" FT /note="ORFY" FT /db_xref="GOA:P39839" FT /db_xref="InterPro:IPR001375" FT /db_xref="InterPro:IPR011042" FT /db_xref="InterPro:IPR011659" FT /db_xref="UniProtKB/Swiss-Prot:P39839" FT /protein_id="CAA28272.1" FT /translation="MPSVPQRQRRAPFFAGDKGVLLETNTCKGGDEYEKLITADDITAI FT VSVTSPQYAPD" XX SQ Sequence 2400 BP; 702 A; 534 C; 627 G; 537 T; 0 other; gaattcatgt aaaagatgag gttggttcat tctcgaaaat tacatctgtg ttctcagagc 60 ggggcgtgac gtttgaaaaa atccttcagc tgccaattaa aggccatgat gagttagctg 120 aaatcgtaat tgtcacacat catacatcag aagctgattt cagtgatatc ctgcaaaacc 180 taaatgattt ggaagtcgtt caagaagtca aaagcacata tcgtgtagaa gggaacggtt 240 ggagctaatg tggaaaggac ttatccatca atataaagaa tttttacctg taacagatca 300 aacaccggcg ctaactttac atgaaggaaa cacacctctt attcacctgc cgaagctgtc 360 tgagcagctc ggaattgagc ttcatgtcaa aacggaaggc gtcaatccta cgggatcatt 420 taaagatcgc ggaatggtta tggctgtggc aaaggcaaaa gaagaaggca atgacacgat 480 tatgtgcgcg tcaacaggta acacttccgc tgctgcggca gcatatgcag cccgtgctaa 540 catgaaatgc attgtcatca tcccgaacgg aaaaattgca tttggaaaac tcgctcaagc 600 tgtcatgtac ggagccgaga ttatcgcaat tgacggaaac tttgacgatg cgcttaaaat 660 tgtccgttcc atctgtgaga aatcaccgat tgcccttgtc aactcagtca acccttaccg 720 cattgaaggc caaaaaactg ctgccttcga agtgtgcgaa cagctggggg aagcgcctga 780 tgttttagcg atcccggtcg gaaatgctgg aaacatcact gcgtactgga agggcttcaa 840 ggaatatcat gagaaaaacg gcacaggcct tccgaaaatg cgcggctttg aagctgaggg 900 tgcggcggca atcgtgcgca atgaagtgat tgaaaatccg gaaacaatag cgacagccat 960 tcgtatcgga aacccggcaa gctgggacaa agctgtaaag gcagccgagg aatccaatgg 1020 gaaaattgac gaagtcactg atgatgaaat ccttcacgca tatcagctga tcgcccgtgt 1080 agaaggcgtg tttgcagaac caggttcttg cgcgtctatc gcaggagtgc tgaaacaggt 1140 gaaatccgga gaaattccga aaggcagcaa ggtcgtagct gtgttaacag gaaacggact 1200 gaaagatccg aacacagcgg tcgacatttc agaaatcaag cctgtcacat tgccgactga 1260 tgaagacagc atccttgaat atgtaaaagg agcggcccgt gtatgaacga agccgacatg 1320 ctgttctctg tcactgttcc cggaagcaca gctaacctag gccccggctt tgattcagtc 1380 ggaatggcgc tcagcagata tttgaagctg accgtctttg aaagcgacaa atggtctttt 1440 gaggctgaaa cagaaacagt cgccggaatt cgcggtacag ataacctgat ctaccaagtg 1500 gctaaacgga ccgcagattt gtacggaaaa gaaatgcctc ctgtccatgt gaaggtgtgg 1560 agcgacatcc cgcttgcacg cggccttggc agcagcgccg cagcgattgt agcggccatt 1620 gaactggctg atgaattatg cggcttaaag ctgtctgaag cggacaagct gcatttagcg 1680 agtctagaag aaggacaccc ggacaatgct ggcgcttctc tcgtcggcgg acttgtgatc 1740 ggcctgcatg aggatgacga gacccaaatg atccgcgtcc cgaatgctga cattgacgta 1800 gtcgttgtca ttccttttta tgaagtgctg acaagagacg cgagagacgt gcttccgaag 1860 gagtttccat atgccgatgc cgtaaaagca agtgctgtca gcaatatcct cattgctgcg 1920 atcatgtcca aggattggcc gcttgtcggg aaaatcatga agaaggatat gttccatcag 1980 ccgtaccggg cgatgcttgt gcctgagctg tcaaaagtag agcacgtcgc cgagatgaag 2040 ggcgcatatg gaacggctct cagcggagca ggcccaacga ttctcgtcat gaccgaaaaa 2100 ggaaagggag aagagctaaa agaacagctc gcgcttcatt tccctcattg tgaagtagac 2160 gctttgaccg ttccgaaaga gggaagtata atagagcgaa atcctttata tcaagtaaaa 2220 agtgtataga tcatgccaag cgttcctcag cgtcagcgga gggcgccttt ttttgctgga 2280 gataaaggag ttttattaga aacgaataca tgtaaaggag gagatgaata tgaaaagctg 2340 ataaccgcag acgacatcac agcgattgtc tctgtgacca gtcctcaata cgccccagac 2400 // |
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