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EBI DbfetchID X03274; SV 1; linear; genomic DNA; STD; PRO; 3393 BP. XX AC X03274; XX DT 02-JUL-1986 (Rel. 09, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Pseudomonas aeruginosa plasmid pMG7 XX KW endonuclease; methylase; plasmid. XX OS Pseudomonas aeruginosa OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid pMG7 XX RN [1] RP 1-3393 RX DOI; 10.1093/nar/13.23.8441 RX PUBMED; 3001639. RA Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., RA Gingeras T.R.; RT "Nucleotide sequence of the PaeR7 restriction/modification system and RT partial characterization of its protein products"; RL Nucleic Acids Res. 13(23):8441-8461(1985). XX CC Wilson G.G. reports an alternative start position at bp 116, which CC increases the length of the peptide from 531 aa to 574 aa. CC A substantial RBS spans nt 99-103. CC (Methods in Enzymology, Vol. 216:259-279 (1992) XX FH Key Location/Qualifiers FH FT source 1..3393 FT /organism="Pseudomonas aeruginosa" FT /plasmid="pMG7" FT /mol_type="genomic DNA" FT /db_xref="taxon:287" FT promoter 146..151 FT /note="put. -35 region" FT promoter 165..171 FT /note="put. -10 region" FT misc_feature 205..217 FT /note="2 tandem PaeR7 recognition sites" FT CDS 241..1836 FT /transl_table=11 FT /note="put. methylase (aa 1-531)" FT /db_xref="GOA:P05103" FT /db_xref="HSSP:2ADM" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P05103" FT /protein_id="CAA27025.1" FT /translation="MVDFILDLAGYTEDQPLHEKRLLEPSFGGGDFLLPIIQRLLSAWR FT AARPNGTEVDDLGDAIRAVELHHDTFRSTYAAVVALLKREGLSANAATALADRWLSQGD FT FLLAPLEGQFDFVVGNPPYVRPELIPAPLLAEYRSRYQTMYDRADIYIPFIERSLTALS FT AGGNLGFICADRWMKNRYGGPLRSLVAERFHLKVYVDMVDTPAFHSDVIAYPAITIISR FT EGGGATRIAHRPSIDRATLTTLAGLLSAPTLPKDAGPVRELARVTNGAERGCWSLLTRW FT RLFAVWRARSHCSKRLGARFGIGVATGADKAFIGDFESLDVEPDRKLPLVTTKDIMTGE FT VQWRGQGVINPFAESGGLVDLGEYPRLRRYLEARRDVIAGRHCAKKAPANWYRTIDRIT FT PALAARPKLLIPDIKGESHIVFEGGELYPSHNLYYVTSDDWDLRALQAVLLSAVSRLFV FT ATYSTKMRGGFLRFQAQYLRRIRIPRWADVPEPLRRELAEAAIKRDVQACNRAVFRLYG FT LSHEERSALGGNGE" FT CDS 1837..2577 FT /transl_table=11 FT /note="put. endonuclease (aa 1-246)" FT /db_xref="GOA:P05104" FT /db_xref="InterPro:IPR007636" FT /db_xref="UniProtKB/Swiss-Prot:P05104" FT /protein_id="CAA27026.1" FT /translation="MALDLVDYEQKARDAVKAFWGNREAARQKQIESGKADQGERAGVT FT GGKNMDGFLALVLDVIKANGLAHAEIHQNRAMLTLPGYFRPTKLWDLLVIYKGELIAAI FT ELKSHVGPSFSNNFNNRTEEAIGTAHDLWTAYREEAFGKQPRPFVGWLMMVEDAPESRR FT PVRDSSPHFPVFEEFKGASYLTRYDLLCQRLVQEQLYTTAAVIAAERSAVDTGNFTELS FT SMTSLKTFVSALAGHIAAEAARLG" XX SQ Sequence 3393 BP; 636 A; 972 C; 1084 G; 701 T; 0 other; cgccgacgtg gcgaaacgct atggcgttta cacggccacc atttacaagc acgttggtgc 60 ggtgcgccca gagcgcggcg catcgccgat gatttagtga ggtcgtcatt catggcattt 120 gcgccaagtg ttgctcataa gcctgttgcc gctgctgtat gccctgtcat ggccgcgacc 180 gaggcgttgg caacggaagg ggggctcgag gctcgagggg ctattttcac gcgctccgag 240 gtggtcgatt tcatcctcga cctggctggc tacaccgagg atcaaccgct gcacgaaaag 300 cggcttttgg aaccgtcgtt cggtggcggg gacttcttgc tgccgatcat tcagcggctg 360 ctgagtgcat ggcgagcagc aaggcccaat ggtactgagg tcgatgattt gggcgacgcc 420 atccgggcgg tggagttgca ccacgacacc ttccgcagca cctacgctgc cgttgtcgcg 480 ctgctcaagc gtgaggggct gtcggcgaat gctgcgacgg ccttggctga ccgctggctg 540 tcgcagggtg atttcctgct ggctccgctg gagggccagt tcgatttcgt ggtgggcaat 600 cctccctacg ttcgacctga gctgattccg gcccctttgc tggccgagta ccgcagccgc 660 tatcagacga tgtatgaccg ggcggacatc tacattccct tcatcgagcg gtcgctgacg 720 gcattgtctg ctggcggcaa tctgggcttt atctgcgcgg atcgctggat gaagaaccgc 780 tacggtgggc cactgcgtag ccttgttgcc gaacggttcc acctgaaagt ctatgtcgat 840 atggtggata caccggcctt ccattccgat gtgatcgcct atccggccat caccatcatc 900 agccgtgagg ggggcggggc gacgcgcatc gcacaccgcc cgtccataga ccgggccacg 960 ctgaccacgc tggctggtct gctgtcggca ccgacacttc cgaaggatgc tggcccggtg 1020 cgggaactcg cccgtgtgac caatggtgcg gagcgtggct gctggagtct tctgaccaga 1080 tggcgcttat tcgccgtctg gagggcgcgt tcccactgct cgaagaggct gggtgcaagg 1140 ttcggcattg gtgttgcgac gggcgctgac aaggcattca tcggcgactt cgagtcgctg 1200 gatgtcgagc ctgatcggaa gctgccgctg gtgacaacca aagacatcat gaccggcgaa 1260 gtgcagtggc gtggtcaggg ggtcatcaac cccttcgccg agtctggagg gctggtcgat 1320 cttggcgagt atccgcgtct gcgccgctac cttgaggctc gccgggatgt gattgccggt 1380 cggcattgcg cgaaaaaggc tcctgccaac tggtatcgca cgattgaccg tattaccccg 1440 gcgctggctg cgaggcccaa gctgttgatc cctgacatca agggtgagtc gcacattgtt 1500 ttcgagggcg gcgagctgta tccaagccac aacctctact acgttacctc ggacgattgg 1560 gatttgcggg ccttgcaagc tgtgctgctg tccgctgtct cgcgcctgtt cgtggccacg 1620 tactcaacga aaatgcgagg tggcttcttg cgcttccaag cgcaatacct gcgccgcatc 1680 cgtatcccac gctgggccga tgtgcctgaa ccgctacgcc gcgagctggc tgaggcagcc 1740 atcaagcggg acgtgcaagc ctgcaaccgg gctgtgttca ggctctacgg gctgagccac 1800 gaagaacgat ctgcccttgg gggcaatgga gaataaatgg cacttgatct tgtcgattac 1860 gaacagaagg cccgcgacgc cgtaaaggcg ttttggggca accgtgaagc tgcacggcaa 1920 aagcagattg agtcaggcaa ggccgaccaa ggtgaacgcg ctggcgtcac aggcggcaag 1980 aacatggacg gattcttggc cttggtgctc gatgtcatca aggccaatgg tctggcccac 2040 gccgagattc accagaaccg ggcgatgctg accctgcccg gctacttccg cccgacaaag 2100 ctgtgggatt tgttggtgat ctacaagggc gagctgatcg cagccatcga actgaaaagc 2160 catgtcgggc catcgttcag caacaatttc aacaaccgta ccgaggaagc catcggaaca 2220 gctcatgacc tctggacagc ctaccgggaa gaggctttcg gcaagcagcc acgtcccttc 2280 gtcggctggc tgatgatggt cgaagatgcg ccggagtccc gccgtcccgt tcgggactcg 2340 tcgccgcatt tcccggtctt cgaggagttc aagggggcat cgtatctcac gcggtatgac 2400 ttgctgtgtc agcgattggt gcaggagcag ctctatacga ctgctgccgt catcgcggca 2460 gaacgcagtg cggtggatac cggcaacttc acggagctgt cgtcgatgac cagcctcaag 2520 acattcgtgt cggctttggc tgggcacatc gccgccgagg cggcacgact gggctgattg 2580 cccgttctcg atcattcggg cttggaggcc ggacgatggt gacaaccaca actgaggcgc 2640 tttcagaggt cttgcgcggg tatggacgcg ctgatgaaac ggcattggca tcactgctgg 2700 aggtcagcga ctgggagcgc atcgctcgtc aggagctgga gcgccgggct acccgaattg 2760 tgcagtcgct tgatgacggc acccttgagg ccatcgcatc tggttcgctc gatgtccggg 2820 ccgtctgccg ccaagtggcc gacgagatca agcagaccgc ttagacgatg gcgctgcctt 2880 ctttccgacc aacgggtggc gcgagggcca gagcgtaaag ccgcgttacc tcgtgctgga 2940 gatcaaagac aaggatcgcc cggatgacaa ggcattggga tgggtgcttg tcgagcggga 3000 ggaaacctat cggcgcgatc ctcgggatgg gacgatctac gaggcgtcca ttcggctttc 3060 ctaccagcgc atcacagcca agttttcgta ccgggatggt ggcaacaggg cgatttgatg 3120 gcagctactc gcgcaacttc aactctgtct cgttgacttc cacctcgatg tcgaaagggg 3180 cgtattcctt gatttgccgg ggctggatgg ccagcgaatc ggcacctact tgatgaatga 3240 gatcgtccag tgggtgcagc agtggcccga gacgaccgtc aatagcatcg agctgctggc 3300 cggacaggcg catggtgaca acaaggcacg gcgcaactgg ttttatgagc agttcggtct 3360 gtcttcgact acaccgaccc cgaggcatcg cga 3393 // |
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