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EBI DbfetchID X03102; SV 1; linear; genomic DNA; STD; FUN; 2812 BP. XX AC X03102; XX DT 28-JAN-1986 (Rel. 08, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 5) XX DE Yeast MEL1 gene for alpha-galactosidase XX KW alpha-galactosidase; inverted repeat; protein binding site; signal peptide. XX OS Saccharomyces cerevisiae (baker's yeast) OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. XX RN [1] RP 1-2812 RX DOI; 10.1093/nar/13.20.7257 RX PUBMED; 2997745. RA Liljestroem P.L.; RT "The nucleotide sequence of the yeast MEL1 gene"; RL Nucleic Acids Res. 13(20):7257-7268(1985). XX DR TRANSFAC; R01020; Y$MEL1_01. DR TRANSFAC; R04148; Y$MEL1_02. XX CC Data kindly reviewed (10-JAN-1986) by P. Liljestroem XX FH Key Location/Qualifiers FH FT source 1..2812 FT /organism="Saccharomyces cerevisiae" FT /mol_type="genomic DNA" FT /db_xref="taxon:4932" FT misc_feature 208..224 FT /note="put. regulatory region, put. GAL4 protein FT recognition sequence" FT repeat_region 215..223 FT /note="inverted repeat" FT repeat_region 238..246 FT /note="inverted repeat" FT promoter 342..348 FT /note="pot. TATA-box" FT promoter 351..357 FT /note="pot. TATA-box" FT misc_feature 408..413 FT /note="minor transcription initiation region" FT misc_feature 437..440 FT /note="major transcription initiation region" FT CDS 461..1876 FT /note="precursor" FT /db_xref="GOA:P04824" FT /db_xref="InterPro:IPR006215" FT /db_xref="UniProtKB/Swiss-Prot:P04824" FT /protein_id="CAA26888.1" FT /translation="MFAFYFLTACISLKGVFGVSPSYNGLGLTPQMGWDNWNTFACDVS FT EQLLLDTADRISDLGLKDMGYKYIILDDCWSSGRDSDGFLVADEQKFPNGMGHVADHLH FT NNSFLFGMYSSAGEYTCAGYPGSLGREEEDAQFFANNRVDYLKYDNCYNKGQFGTPEIS FT YHRYKAMSDALNKTGRPIFYSLCNWGQDLTFYWGSGIANSWRMSGDVTAEFTRPDSRCP FT CDGDEYDCKYAGFHCSIMNILNKAAPMGQNAGVGGWNDLDNLEVGVGNLTDDEEKAHFS FT MWAMVKSPLIIGANVNNLKASSYSIYSQASVIAINQDSNGIPATRVWRYYVSDTDEYGQ FT GEIQMWSGPLDNGDQVVALLNGGSVSRPMNTTLEEIFFDSNLGSKKLTSTWDIYDLWAN FT RVDNSTASAILGRNKTATGILYNATEQSYKDGLSKNDTRLFGQKIGSLSPNAILNTTVP FT AHGIAFYRLRPSS" FT sig_peptide 461..514 FT /note="put. signal peptide (aa -18 to -1)" FT mat_peptide 515..1873 FT /note="alpha-galactosidase (aa 1-453)" FT misc_feature 773..775 FT /note="pot. glycosylation site" FT misc_feature 983..985 FT /note="pot. glycosylation site" FT misc_feature 1268..1270 FT /note="pot. glycosylation site" FT misc_feature 1568..1570 FT /note="pot. glycosylation site" FT misc_feature 1667..1669 FT /note="pot. glycosylation site" FT misc_feature 1697..1699 FT /note="pot. glycosylation site" FT misc_feature 1724..1726 FT /note="pot. glycosylation site" FT misc_feature 1763..1765 FT /note="pot. glycosylation site" FT misc_feature 1820..1822 FT /note="pot. glycosylation site" FT misc_signal 1968..1975 FT /note="pot. transcription termination signal" FT misc_signal 1987..1989 FT /note="pot. transcription termination signal" FT misc_signal 2007..2009 FT /note="pot. transcription termination signal" FT misc_signal 2017..2019 FT /note="pot. transcription termination signal" XX SQ Sequence 2812 BP; 831 A; 543 C; 607 G; 831 T; 0 other; gtcgacttct aagtaaacac cattactagg agatgactaa atctggaaaa ccacatggtg 60 gtctgaatgc gtctagtctc tgccataaac ataacatgtt tgttttaatg cattctcgtg 120 tttaatcgac attaatgtgg ggggagaaag acatcccatc cctgaaaggt ttttccaggg 180 aatagtcagg acgcattggc tttcattcgg ccatatgtct tccgaaagaa gaagaaagga 240 agacatgtat tacattatcc aacaaaaaat ggttcttgac gtctacaaat caagaatctt 300 aaagacattg aacgaagtag ctgaataaaa attatgaaaa ctataaaaac tataaaaact 360 gtacttaagt cctcaataaa acataaactt cttactgtat aaggttttcg ataatttctt 420 acttgattct aggagagcaa cggtaataaa agcaacgacg atgtttgctt tctactttct 480 caccgcatgc atcagtttga agggcgtttt tggggtgtct ccgagttaca atggccttgg 540 tctcactcca cagatgggtt gggacaactg gaatacgttt gcctgcgatg tcagtgaaca 600 gctacttcta gacaccgctg atagaatttc tgacttgggg ctaaaggata tgggttacaa 660 gtatatcatt ctggatgact gctggtctag cggcagagat tccgacggtt tcctcgttgc 720 agatgaacaa aaatttccca atggtatggg ccatgttgca gaccacctgc ataataacag 780 ctttcttttc ggtatgtatt cgtctgctgg tgagtacacc tgtgctggat atcctgggtc 840 tctgggtcgt gaggaagaag atgcacagtt ctttgcaaat aaccgcgttg actacttgaa 900 gtacgataat tgttacaata agggtcagtt tggtacaccg gaaatttctt accaccgtta 960 caaggccatg tcagatgctt tgaataaaac tggtaggcct atattctatt ctctatgtaa 1020 ctggggtcag gatttaacat tttactgggg ctctggtatc gccaattctt ggagaatgag 1080 tggagatgtt actgctgagt tcactcgtcc agatagcaga tgtccctgtg atggcgatga 1140 atacgattgc aagtacgccg gtttccattg ttctattatg aatattctta acaaggcagc 1200 tccaatgggg caaaatgcag gtgttggtgg ttggaatgat ctggacaatc tagaggttgg 1260 tgtcgggaat ttgactgacg atgaggaaaa ggcacatttc tctatgtggg caatggtaaa 1320 gtctccactt atcattggtg ccaatgtgaa taacttaaag gcatcttcgt actcaatcta 1380 tagtcaagcc tctgtcatcg caattaatca agattcaaat ggtattccag caacaagagt 1440 ctggagatat tatgtttcag acacagatga atatggacaa ggtgaaattc aaatgtggag 1500 tggtcctctt gacaatggtg atcaagtggt tgctttattg aatggaggaa gcgtatctag 1560 accaatgaac acgaccttgg aagagatttt ttttgacagc aatctgggtt caaagaaact 1620 gacatcgact tgggatatct acgacctatg ggccaacaga gttgacaact cgacagcgtc 1680 tgctatcctt ggacggaata agacagccac cggtattctc tacaatgcta cggagcaatc 1740 ctacaaagac ggtttgtcta agaatgatac aagactgttt ggtcagaaaa ttggtagtct 1800 ttctccaaat gctatactta acacgactgt tccagctcac ggtatcgcct tctataggtt 1860 gagaccctct tcttgagctt attgttgagc aaagcagggc gagaagtatt gatgattgtt 1920 aaaaagttca tgaaaaaaat actactcgaa tatttattca gagtaactaa ataataaacg 1980 acagaatagc ctatcaggta ttccaatagt tttcgttttg taggtacata atctgaagcc 2040 cttgaacttt ttctcgttta catacttcat tgcattagcg atatttcaca tgtgctatac 2100 tagtgacttt tgtaaaatac ttttctgcca atgtgattct agaattgtat gacatttcac 2160 gaagaggaac aacagcttca ggagtacata caaacgacga aatcatcata tcagcaaaac 2220 cggatatcga agaagcagaa ggcgctagtc tacaggtatc tctgtagttg atacaaacaa 2280 aaacaaagag ttcgaacaca tttctgggtc ccgagtagtg gcgttggtgt tccaccattg 2340 gtcgaacaat tgttttttgc taccgctcgc tcatagccgg tttcagtaaa attgaagtaa 2400 gcccagtttc atctgaattt gagaataggg tctaaaaagg gagtatcacc atcgaacgct 2460 tcctgatagt ccttagagaa cagtcatcag atgtccatat attactacta cccttctaga 2520 cctgaccacg tactgttgga tgcattggta agaatttttg cccgtattat cttcaatttt 2580 agaataaaca tctaacttgg ggttagtagc gttcacaaat atgccaacat tgttgacaga 2640 cttgttgaac atctcaagtt cttcaggact agcacggctt tgagtgaagc ccacttgttc 2700 ttctttgatg tcgaaatcgc acacagtgtt caggaagaaa ttgtagctct tcgaaatgtt 2760 ttcgttctca ctctaccagg cttcaccaag cactctgaga aactcgggat cc 2812 // |
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