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EBI DbfetchID X02988; SV 1; linear; genomic DNA; STD; PRO; 4253 BP. XX AC X02988; XX DT 28-JAN-1986 (Rel. 08, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 5) XX DE Bacillus subtilis BsuRI restriction-modification genes XX KW endonuclease; inverted repeat; methylase; modification enzyme; nuclease. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4253 RX DOI; 10.1093/nar/13.18.6403 RX PUBMED; 2997708. RA Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.; RT "Nucleotide sequence of the BsuRI restriction-modification system"; RL Nucleic Acids Res. 13(18):6403-6421(1985). XX FH Key Location/Qualifiers FH FT source 1..4253 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT promoter 224..229 FT /note="put. TATA-like sequence" FT misc_feature 235..235 FT /note="transcription start site" FT misc_feature 236..236 FT /note="altern. transcript start site" FT RBS 284..288 FT /note="put. rRNA-binding site" FT CDS 297..2027 FT /transl_table=11 FT /note="BsuRI nuclease (aa 1-576)" FT /db_xref="GOA:P06529" FT /db_xref="UniProtKB/Swiss-Prot:P06529" FT /protein_id="CAA26730.1" FT /translation="MGKNSKAIGNNHVKSVYQALLQSLKSKSVNGFSKITIETISFIKN FT LYPEIDSVTSKFDNSRPDQSKDLTLYLKSGETISLNLFLIKKGRRIQPKNAGAKSFLEK FT YFLSAEMQKIFNKEFERYYLDYLKEVVEHKKGTHYITDKRELKRLVSSHFPKFTEEINL FT YRDKFLFNLRETCFTLLQQFYNEKNIGFTHAFNVFFMVNDTNIITSYGKDENDVKVEKF FT APASPSLKDIELYKTGKSTVGIKFGEVGLTLRFKFESDPWKSIKLATGYHEFPKEKERV FT NVNLKTMRRMEKLLNKHEYAKTSNNSNAIGKCHEAWTYYYFLKAFPDVIQVDPKQCVEL FT INTYFSSINQNTLKKLYSSTSTIVDAITEKLRQKYHDYIIESIELIPDAYIKDRLDTGD FT LQLVLKVNNNIIVENISLKALAKRNSKITTKNPGMGSILGPTYFNMGSMESVINEVKNK FT FTIGEFNHRKSLEILSYEFGMKLDSATQEQLRRGIHNLLGKAMIAITIYGEGISFCKEP FT SEIDGEVKVHVNVPSAIQNTLTWNNELESISLRAKFSKSQKHGWSSIKLTSECQLESRK FT " FT promoter 2768..2773 FT /note="put. TATA-like sequence" FT misc_feature 2779..2779 FT /note="transcription start site" FT RBS 2781..2784 FT /note="put. rRNA-binding site" FT CDS 2808..4118 FT /transl_table=11 FT /note="BsuRI methylase (aa 1-436)" FT /db_xref="GOA:P06530" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P06530" FT /protein_id="CAA26731.1" FT /translation="MTLKIDIKGRGKYKPASDYSIDDVKNVLMEKIFEESSRIINSDDD FT LEIIEKVDFRTDKINVLSLFSGCGGLDLGFELAGLAAVIGEQAAMEAFKDKDRFNELRN FT KSIFHTIYTNDLFKEANQTYKTNFPGHVIQHEKDIRQVKYFPKCNLILGGFPCPGFSEA FT GPRLIDDDRNFLYLHFIRSLIQAQPEIFVAENVKGMMTLGKGEVLNQIIEDFASAGYRV FT QFKLLNARDYGVPQLRERVIIEGVRKDISFNYKYPSPTHGEETGLKPFKTLRDSIGDLV FT TDPGPYFTGSYSSIYMSRNRKKSWDEQSFTIQASGRQAPLHPGGLSMKKIGKDKWVFPD FT GEENHRRLSVKEIARVQTFPDWFQFSQGTNSQTSINNRLDKQYKQIGNAVPVLLAKAVA FT SPIANWAINYLESSPNNKIKNRERKLSIRTFLRIKTS" FT misc_signal 4140..4174 FT /note="pot. stem loop structure, pot. transcription FT terminator" FT repeat_region 4140..4150 FT /note="inverted repeat" FT repeat_region 4164..4174 FT /note="inverted repeat" XX SQ Sequence 4253 BP; 1567 A; 650 C; 782 G; 1254 T; 0 other; gaattccctt tattcccgcc ttcataaatt gcatctatac ggagaggggc attttctaaa 60 ctttcaaatg ggtaaattga attaagtgtg tttgtttctt tcaacttctg aactctcctt 120 cagttagcat gttaacattt acaatttata ttataagctt ttacgagaac cacttggaag 180 ctttggtttt taggaaatca taacttattt agattacttg tggtactatt tacttagtta 240 tcttttccac tttttcaaga aaacataaga gaaatagaaa acagagggtg tatacaatgg 300 ggaaaaattc aaaggctatc ggtaacaatc atgttaaatc agtataccaa gcattattgc 360 aatctttaaa atcaaaatct gtaaacggtt tttctaagat aacaatagag acaatatcat 420 ttattaaaaa tttatatcca gaaattgatt ctgtaacttc aaagttcgat aattcacgtc 480 ctgatcaatc aaaagatctt actttatatt tgaaaagtgg cgaaactatc tcgttgaatc 540 tatttctgat taaaaaaggc cgacgcattc agcctaaaaa tgctggcgcg aagagttttt 600 tagaaaagta ctttttatca gcagaaatgc aaaagatttt taacaaagaa tttgaaaggt 660 actatttaga ctacttaaaa gaagtagtgg agcataaaaa aggaacacac tacataacag 720 ataaaagaga gttaaaaaga cttgtgtcaa gccattttcc aaaattcaca gaagaaatta 780 atttatatag agacaagttt cttttcaact tgcgtgaaac ttgttttacg ttgttgcagc 840 aattttataa tgagaagaat ataggattta cccatgcctt taatgtcttc ttcatggtca 900 atgatacaaa tatcattaca agttacggca aagatgaaaa tgatgtcaaa gttgaaaagt 960 ttgcacctgc atccccatct ttgaaggata ttgaacttta caaaacagga aagagtacag 1020 ttgggattaa atttggagaa gtgggactca ccctaagatt taaatttgaa agtgatcctt 1080 ggaaatcgat taaacttgct acaggttacc atgaattccc taaagaaaaa gagagagtga 1140 atgtcaactt aaaaacaatg aggagaatgg aaaaactatt gaataaacat gagtacgcta 1200 aaacatcgaa taacagtaac gcaataggca aatgccatga agcatggaca tattattatt 1260 tcttaaaggc gtttcccgac gttatacagg tagatccaaa gcaatgtgtt gaattaatta 1320 acacatattt ttctagtata aatcaaaata cactaaaaaa actatatagc tcaacttcta 1380 caatagtaga tgctattaca gagaaactaa gacaaaaata tcatgactat attatagaaa 1440 gtatcgaatt aatcccggat gcatatataa aagataggct tgatacagga gatcttcagt 1500 tagtcttaaa agtaaataac aatattattg ttgagaatat ttctctaaaa gctttagcaa 1560 aaaggaatag caaaattact acaaagaatc cgggtatggg aagcattctt ggaccaacat 1620 attttaatat gggaagcatg gaatctgtta ttaatgaagt gaaaaacaag tttaccatag 1680 gggaatttaa tcatagaaaa agtttagaaa tactctctta tgagttcgga atgaaacttg 1740 acagtgcaac tcaggaacaa ttaagaagag gaattcacaa tttattagga aaagcaatga 1800 tagctattac tatttatggt gagggaatta gcttttgcaa agagccttct gaaattgacg 1860 gtgaagtaaa agtgcatgtt aatgtgcctt ctgctataca aaatacctta acatggaata 1920 atgagttaga gtcaattagt ttacgtgcaa aatttagtaa aagtcaaaag cacggctggt 1980 cttctattaa attaacatca gaatgccagt tggaaagcag gaaataaaca atgtccaatg 2040 attatatgcc aataacctct tttgtttttt ctatctttta tgagggaata ttataaatga 2100 ttccaaaaga aaggttgggg caaatggaca agatgtcaaa agagtctaga agcaatgtta 2160 tgaaatccat taaatcagtt tcccaattag aaaatctggt tgcaagtgca ttgtggaatc 2220 ggggctatcg gttccgaaga aacaccaaaa gcctttttgg gaaaccagat ttgtctataa 2280 aaaagtataa ggtggtcatt tttatcgatt cttgtttctg gcatttctgc cctgttcacg 2340 gcaggatccc taaaagcaat acagattatt ggaatgctaa atatataaaa aataaaaccc 2400 gagatgaaga agtcaacaca ttttataggg agaataattg gaatatactt cgtgtatggg 2460 aacatgaatt caaggaagat tttgattttg ccattgacac gatagctaat tttattgaac 2520 aatctaagag aaaataaaga gcaaaattag attaaaaaga actttcttgt ggaatgtttt 2580 gaacaaatcc atcttatatt atgacctcgc tttcctccat taaatcaatg gctagcaatg 2640 tttctaattt aattcaattt cccaattaac agaataacac aaaataacca ttaaataata 2700 atgtaaacat ataaggaaaa tcattatatt aaggacactg gcgctggcct tttgaattga 2760 aatttgataa gatatcataa gaggtgcaac aggaggttgt ttaaaatatg actttaaaaa 2820 ttgatatcaa aggtagaggc aaatataagc cggcgtctga ttattctata gatgatgtaa 2880 agaatgtact aatggagaag atctttgagg aatcttcaag aattattaat tctgatgatg 2940 atctagaaat cattgaaaag gttgattttc gcactgataa aataaatgtg cttagcctgt 3000 tttcgggctg cggcggactc gaccttggat ttgaattagc aggattggct gcagtaatcg 3060 gtgaacaagc agctatggaa gcatttaaag ataaagaccg atttaatgaa ctaaggaata 3120 aaagcatctt ccataccata tatacaaatg acttgtttaa agaagccaat caaacataca 3180 aaacaaattt tccaggtcat gttatacagc acgaaaagga tataagacaa gttaaatatt 3240 tcccaaaatg caaccttatc cttggaggat tcccctgccc tggatttagt gaagctggcc 3300 cacgtttgat agatgacgat cgtaacttct tatatttaca ttttattaga agcctgatac 3360 aagcacagcc agaaatattt gtagcagaga atgttaaagg catgatgact cttggaaaag 3420 gcgaggtttt aaatcagatt atcgaagatt ttgcttcagc tggctatagg gttcagttta 3480 agctattaaa tgcaagggac tatggagttc cgcaactcag agagcgtgtt ataattgaag 3540 gcgtcagaaa agacattagc ttcaattaca aatatccatc tccaacccat ggtgaagaaa 3600 cgggcctaaa gccattcaaa acgctgagag attctatagg agatttagtg actgatccag 3660 gaccgtactt tacggggtca tattcttcta tttatatgtc tcgtaacaga aagaaaagct 3720 gggacgagca aagctttacc atccaagctt cagggagaca ggcccccctc catccaggtg 3780 gcttatctat gaaaaaaata ggaaaagata agtgggtttt ccctgatggg gaagaaaacc 3840 atagaaggtt gtctgtaaag gaaattgcca gggtgcaaac tttcccagat tggtttcaat 3900 ttagtcaagg aacaaacagc cagacttcaa ttaacaatag actagacaaa caatacaagc 3960 aaatagggaa tgctgtgcca gttttgctgg ctaaggcagt tgcttctcct attgcaaatt 4020 gggcaataaa ttatctcgaa agctctccaa ataataaaat aaagaacaga gaacgcaagc 4080 tttcaattag gactttttta agaatcaaaa ccagttaaat tgaatgcttt cctttaatta 4140 actagccatc cctagcaaaa taagatggct agtttttttt gtaaagctag cttttgacaa 4200 gaaggagaac atacttaaat atgtttgcca aaaagaagga tgggctttat act 4253 // |
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