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EBI DbfetchID X02587; SV 1; linear; genomic DNA; STD; PRO; 3003 BP. XX AC X02587; XX DT 02-JUL-1986 (Rel. 09, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 7) XX DE M.smithii DNA with insertion element ISM1 XX KW insertion sequence; plasmid; unidentified reading frame. XX OS Methanobrevibacter smithii OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanobrevibacter. XX RN [1] RP 1-3003 RX DOI; 10.1007/BF00383311 RX PUBMED; 2993814. RA Hamilton P.T., Reeve J.N.; RT "Structure of genes and an insertion element in the methane producing RT archaebacterium Methanobrevibacter smithii"; RL Mol. Gen. Genet. 200(1):47-59(1985). XX FH Key Location/Qualifiers FH FT source 1..3003 FT /organism="Methanobrevibacter smithii" FT /mol_type="genomic DNA" FT /db_xref="taxon:2173" FT repeat_region 556..563 FT /rpt_type=DIRECT FT /note="1" FT repeat_region 564..1944 FT /mobile_element="insertion sequence:ISM 1" FT repeat_region 564..592 FT /note="inverted repeat A" FT promoter 613..621 FT /note="putative" FT /note="ORFIS" FT promoter 629..637 FT /note="putative" FT /note="ORFIS" FT promoter 655..666 FT /note="putative" FT /note="ORFIS" FT RBS 697..705 FT /note="putative" FT /note="ORFIS" FT CDS 717..1922 FT /transl_table=11 FT /note="ORFIS" FT /db_xref="GOA:P22344" FT /db_xref="UniProtKB/Swiss-Prot:P22344" FT /protein_id="CAA26424.1" FT /translation="MTKQNKSALNSNIDFIQLKLYDFFDEKIDQEKIISKRLNKTPNFE FT NTNHKLFLDENNTFKYDNPICPVCGSHKIIKKGTIKKNKQNTNGKTTEFKEQQYQCKKC FT GKKFGIYNNPLIGENKQFLQEIMDKIPGIMKIGYQSLRKISKYFEIFLGIRISHQTIKN FT WSDKNHEESISNEKFEYSGYYLYDEQFLRLNGTRHYRLTLFDAILNIPVTERIVRRRIP FT KNTKKFILESTENKPFICLTTDLFPMYRNVADEIEVKHQLCIFHLFQTINHKLKVYCRR FT NKINGKQRDHIYENAQELKNCFRQNSKKEAIEQFKQYLQKYTAIPVVLKDFIRKHIINH FT FHRYVEYLDDENIEKTSNKVENYYRQTNPEKIKKIYKTKNGILTFLDYQMQNWTEKHIK FT IK" FT repeat_region complement(194..1916) FT /note="inverted repeat A'" FT repeat_region 1945..1952 FT /rpt_type=DIRECT FT CDS complement(1993..2748) FT /transl_table=11 FT /note="ORFC" FT /db_xref="GOA:P22350" FT /db_xref="InterPro:IPR000304" FT /db_xref="UniProtKB/Swiss-Prot:P22350" FT /protein_id="CAA26425.1" FT /translation="MNLGIIGYGNIGELLSQNIISHDFCNLNKLYIANRTLSKINHLKD FT IDPRISITDDNIEVAKTCEKIIISVKTPDLAIVLDPLKPHITKNQQIIHTCAGTDLEFK FT DCGLSCVIPTISSTYDEDNPKKGVSIIMHDENVSGENREFVEKLFSKFSQIKVVDSPMD FT LEIATIAASCMPAFIALGVDLFAGELEEKCNLSKEETFKILAETLNSTAYILKEDIYSP FT DELINKVATKNGITQKGLDVLDKRPARYL" FT RBS complement(2754..2762) FT /note="putative" FT /note="ORFC" FT promoter complement(2778..2783) FT /note="putative" FT /note="ORFC" FT promoter complement(2800..2805) FT /note="ORFC" FT /note="putative" FT CDS complement(2754..>3003) FT /codon_start=2 FT /transl_table=11 FT /note="unidentified reading frame" FT /db_xref="GOA:Q50429" FT /db_xref="InterPro:IPR007183" FT /db_xref="UniProtKB/Swiss-Prot:Q50429" FT /protein_id="CAA26426.1" FT /translation="SFGYADSVTVLSKKASVADGLATKIANEAVGQNSEYKVSNALEAS FT ENYKDLFEGVLIISQDNVGSIGKLPKIVETEEFNVKM" XX SQ Sequence 3003 BP; 1122 A; 434 C; 407 G; 1040 T; 0 other; atcgattgat tcctgggtga ctggaaggta atgttctccg tcaactaaac agagcatttt 60 actaatactt ttcatattaa tcacaattag aatttaatca attagtattt atgataagta 120 atagtattaa aatatttgaa tttataaaag aagaaatggg ctaatattta gcatttatat 180 agttttgaga cctttcaaca aggtcggtta atgaattgca cacattgtca ggtattgaat 240 tttcatcttt ttcgtttatt gacctgtgca attattttac aaagaacaaa aatagcatgt 300 ttgtgttctg cttttgtttt gtggatgtgg tgtgggctta ttttaagtga tatgtattca 360 ctgcaatcgt tttttgctct gatcgttttc tgctaaatat tttaaaatat aaactaaaaa 420 ttgatgtaat tgtatcattt catctttgta catgtgtatc tgacctagta tctataatta 480 agagtttaag taaattattt aaatgaaata aataattagt tttaattatt aatattaatt 540 aatattatta ttttaatctt attgagtctg taaaatttta taggcttatt tgttttttaa 600 ttttttacag ttgaactttc attttgatga aatttcattc taatttttct ttaattttaa 660 tataaatgat ttaaaaatag taaaatactt taataagtga ggacaaataa tattttatga 720 ccaaacaaaa caaatctgcc ctcaatagta atatagactt catacaactt aaactttatg 780 atttttttga tgaaaaaatt gatcaagaaa aaattatttc aaaaagattg aataaaacac 840 ctaattttga aaatactaat cataaactat ttttagatga aaataatact tttaaatatg 900 ataatcccat ttgtccagtt tgtggaagcc acaaaataat caaaaaaggc acaataaaga 960 aaaacaaaca aaatactaat ggaaaaacaa cagaattcaa agaacagcaa tatcaatgca 1020 aaaaatgtgg aaaaaaattc ggaatataca ataatccatt aattggtgaa aataaacaat 1080 ttttacaaga aataatggat aaaattccag gaataatgaa aatagggtac caatcacttc 1140 gtaaaataag taaatacttt gaaatattcc ttggaatcag aatatctcat caaacaatca 1200 agaactggtc tgacaaaaat cacgaagaaa gcatcagcaa tgaaaaattt gaatattctg 1260 gctattattt atatgatgag caatttttaa gacttaatgg aactagacac tacagattaa 1320 ctttatttga tgcaatactc aatattccag tcacagaacg aatagtacgt cgcagaatac 1380 caaaaaacac gaaaaaattt atcttagaat caacagaaaa taaaccattc atttgcctaa 1440 caaccgattt attcccaatg tatcgtaatg tagcagatga aatagaagta aaacatcaat 1500 tgtgcatatt tcatctgttt caaacaataa accataaatt aaaagtatat tgtagaagaa 1560 acaagattaa tggaaaacaa agagaccata tttacgaaaa tgcacaagaa ttaaaaaact 1620 gtttcagaca aaactcaaaa aaagaagcaa tagaacaatt taaacaatat ttacaaaaat 1680 atacggccat accagttgtt ttaaaagatt tcataagaaa acatatcata aatcacttcc 1740 acagatacgt agaatatctt gatgatgaaa atatagaaaa aacatcaaat aaagtcgaaa 1800 attactacag acaaaccaat cctgaaaaaa taaagaaaat atacaaaacc aaaaatggaa 1860 tcctgacatt tttagactat caaatgcaaa attggactga aaaacacatt aaaatcaaat 1920 aagcctataa aattttacag actcatctta tttaaatatt gcattggtta cagaagttta 1980 gatattaacc ttttataaat atctggcagg tcttttgtct aaaacatcta atcctttttg 2040 agttattccg tttttagttg caactttatt aatcagttca tcaggtgagt aaatatcttc 2100 ttttaatatg tatgccgtac tgtttaatgt ttctgcaagt attttaaagg tttcttcttt 2160 tgaaagattg catttctctt caagttcgcc agcaaaaaga tctacaccaa gagctataaa 2220 tgcaggcatg cagctggcag ctattgtagc tatctccaaa tccatgggac tgtcaacaac 2280 ttttatctgg ctgaattttg aaaatagctt ttcgacaaat tctctatttt cgccagaaac 2340 attttcatca tgcataataa tggaaacacc ttttttagga ttgtcttcat catatgtaga 2400 agatattgtt ggaataacgc agcttaaacc acagtcttta aattccaagt ctgttcctgc 2460 acaggtatgt atgatttgtt gatttttagt tatatgtggt tttagcgggt ctaaaacaat 2520 tgccaaatcg ggagttttta ctgatattat tattttttca caggtttttg caacttctat 2580 gttatcatct gtaattgata tgcgcgggtc aatgtctttt aagtgattta ttttagatag 2640 tgttctattt gcaatgtaga gtttatttaa attacaaaaa tcatgactga ttatattttg 2700 gctgagaagt tctccaatat ttccatatcc aataattccg aggttcatat atttcacatc 2760 ttaacattaa attcttcagt ttcaacaatt tttggaagtt ttccaatgct tccaacatta 2820 tcctgggaaa tgattaaaac tccctcaaac aggtctttat agttttcact agcttcaaga 2880 gcatttgata ctttatattc actgttttgc ccaacagctt cattggctat tttggtagca 2940 agcccgtcag ctactgatgc ttttttactc agcactgtaa cgctgtctgc ataaccgaag 3000 ctt 3003 // |
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