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EBI DbfetchID U97665; SV 1; linear; genomic DNA; STD; PRO; 4665 BP. XX AC U97665; XX DT 18-NOV-1997 (Rel. 53, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Erwinia cypripedii membrane-bound gluconate dehydrogenase complex, subunit DE III precursor, dehydrogenase subunit precursor and cytochrome c precursor, DE genes, complete cds. XX KW . XX OS Pectobacterium cypripedii OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Pectobacterium. XX RN [1] RP 1-4665 RX PUBMED; 9352901. RA Yum D.Y., Lee Y.P., Pan J.G.; RT "Cloning and expression of a gene cluster encoding three subunits of RT membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 RT in Escherichia coli"; RL J. Bacteriol. 179(21):6566-6572(1997). XX RN [2] RP 1-4665 RA Yum D.-Y., Pan J.-G.; RT ; RL Submitted (16-APR-1997) to the EMBL/GenBank/DDBJ databases. RL Bioprocess Engineering Division, Korea Research Institute of Bioscience and RL Biotechnology, P. O. Box 115, Yusong, Taejon, Choongnam 305-600, Korea XX FH Key Location/Qualifiers FH FT source 1..4665 FT /organism="Pectobacterium cypripedii" FT /strain="ATCC29267" FT /mol_type="genomic DNA" FT /db_xref="taxon:55209" FT RBS 247..250 FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 258..920 FT /codon_start=1 FT /transl_table=11 FT /product="subunit III precursor" FT /note="part of gluconate dehydrogenase complex" FT /db_xref="GOA:O34213" FT /db_xref="InterPro:IPR017909" FT /db_xref="UniProtKB/Swiss-Prot:O34213" FT /protein_id="AAC45883.1" FT /translation="MSEHKNGHTRRDFLLRTITLAPAMAVGSTAMGALVAPMAAGAAEQ FT SSGSQTARDYQPTWFTAEEFAFITAAVARLIPNDERGPGALEAGVPEFIDRQMNTPYAL FT GSNWYMQGPFNPDLPKELGYQLPLVPQQIYRLGLADADSWSKHQHGKVFAELSGDQQDA FT LLSDFESGKAEFTQLPAKTFFSFLLQNTREGYFTRSDPRWQSGHGGLEADWLPRRTR" FT sig_peptide 258..383 FT /experiment="experimental evidence, no additional details FT recorded" FT mat_peptide 384..917 FT /product="subunit III" FT /note="part of gluconate dehydrogenase complex" FT RBS 925..929 FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 934..2781 FT /codon_start=1 FT /transl_table=11 FT /product="dehydrogenase subunit precursor" FT /note="part of gluconate dehydrogenase complex; putative FT FAD binding site" FT /db_xref="GOA:O34214" FT /db_xref="InterPro:IPR000172" FT /db_xref="UniProtKB/Swiss-Prot:O34214" FT /protein_id="AAC45885.1" FT /translation="MERGERVSVPVSGYSRGEGVTVANELKKVDAVVVGFGWAGAIMAK FT ELTEAGLNVVALERGPHRDTYPDGAYPQSIDELTYNIRKKLFQDLSKSTVTIRHDASQT FT AVPYRQLAAFLPGTGTGGAGLHWSGVHFRVDPVELNLRSHYEARYGKNFIPEGMTIQDF FT GVSYNELEPFFDQAEKVFGTSGSAWTIKGKMIGKEKGGNFYAPDRSSDFPLPAQKRTYS FT AQLFAQAAESVGYHPYDMPSANTSGPYTNTYGAQMGPCNFCGYCSGYACYMYSKASPNV FT NILPALRQEPKFELRNNAYVLRVNLTGDKKRATGVTYLDGQGREVVQPADLVILSAFQF FT HNVHLMLLSGIGQPYNPITNEGVVGRNFAYQNISTLKALFDKNTTTNPFIGAGGAGVAV FT DDFNADNFDHGPYGFVGGSPFWVNQAGTKPVSGLPTPKGTPNWGSQWKAAVADTYNHHI FT SMDAHGAHQSYRANYLDLDPNYKNVYGQPLLRMTFDWQDNDIRMAQFMVGKMRKITEAM FT NPKMIIGGAKGPGTHFDTTVYQTTHMSGGAIMGEDPKTSAVNRYLQSWDVPNVFVPGAS FT AFPQGLGYNPTGMVAALTYWSAKAIREQYLKNPGPLVQA" FT sig_peptide 934..999 FT /experiment="experimental evidence, no additional details FT recorded" FT mat_peptide 1000..2778 FT /product="dehydrogenase subunit" FT /note="part of gluconate dehydrogenase complex" FT misc_feature 1021..1113 FT /note="encodes putative FAD binding site" FT /inference="non-experimental evidence, no additional FT details recorded" FT RBS 2781..2784 FT /inference="non-experimental evidence, no additional FT details recorded" FT sig_peptide 2793..2849 FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 2793..4118 FT /codon_start=1 FT /transl_table=11 FT /product="cytochrome c precursor" FT /note="part of gluconate dehydrogenase complex" FT /db_xref="GOA:O34215" FT /db_xref="HSSP:1C6S" FT /db_xref="InterPro:IPR014353" FT /db_xref="UniProtKB/Swiss-Prot:O34215" FT /protein_id="AAC45884.1" FT /translation="MMKSILALVLGTLSFAALADDQANDALVKRGEYLARAGDCVACHS FT VKGGQPFAGGLPMATPIGTIYSTNITPDKTTGIGDYSYDDFQKAVRHGVAKNGDTLYPA FT MPYPSYAVVSDEDMKALYAYFMHGVAPVAQANKDSDIPWPLSMRWPLAIWRGVFAPDVK FT AFQPAAQEDPVLARGRYLVEGLGHCGACHTPRSITMQEKALSNDGAHDYLSGSSAPIDG FT WTASNLRGDNRDGLGRWSEDDLRQFLRYGRNDHTAAFGGMTDVVEHSLQHLSDDDITAI FT ARYLKSLGAKDASQTVFTQDDQVAKALWKGDDSQTGASVYVDSCAACHKTDGSRLSALL FT PGAAWQPGGAGEPDPTSLIHIVLTGGTLPGVQGAPTAITMPAFGWRLNDQQVADVVNFI FT RGSWGNGAKATVTAKDVASLRKDETVQAHQGNADIKVLEQQQ" FT mat_peptide 2850..4115 FT /product="cytochrome c" FT /note="part of gluconate dehydrogenase complex" XX SQ Sequence 4665 BP; 989 A; 1330 C; 1417 G; 929 T; 0 other; aggccttaac tgtctgtagg ctgttgtatc agaccatgac aatgtcgcgc ctgcggtgta 60 aagccgctgc gcaaaatgtt aattattttg tgcgaatttg tgtccttacg ctaaatcttt 120 gtcatcaacg gtgttacact gcgacgcaat gttaccggta acggtggcgc tgtatcctta 180 agcccgcaca taaaaatcat tacaacgcaa tcagttaacc tttcatgcca cattatatgc 240 ggcactgagg caatgtcatg tcagaacaca aaaatggtca cacacgcagg gattttctgc 300 tgagaaccat caccctggcg ccagcaatgg cggtgggttc aacagcgatg ggtgcactgg 360 ttgcgccaat ggctgccgga gcagcagaac aaagcagcgg gtcacaaacc gcccgcgact 420 atcagccgac ctggtttacg gcggaagagt ttgcctttat caccgcagcg gtggcacgtc 480 tgatccccaa cgatgaacgt ggtcctggcg cactggaagc cggggtgccg gagtttatcg 540 atcgccagat gaacaccccg tacgccctcg gcagcaactg gtacatgcag gggccgttca 600 atcccgatct gccgaaagag ctgggttatc agctgccgct ggtgccgcag cagatctacc 660 gtctgggcct cgccgatgct gatagctgga gcaaacacca gcacggcaaa gtgtttgctg 720 agctgagcgg cgaccagcag gatgccctgc tgagcgactt cgaaagtggc aaagcggagt 780 tcacccagct cccggccaaa accttcttct ccttcctgct gcaaaacacc cgcgagggtt 840 acttcacgcg atccgatcca cggtggcaat cagggcatgg tgggctggaa gctgattggc 900 ttccccggcg cacgcgctga ttacatggat tgggtggaac gcggtgaacg cgtatccgtt 960 cccgtcagtg gatattcgcg gggagagggc gtaaccgtgg caaatgaatt gaagaaagtg 1020 gatgcggtgg tggtgggttt cggctgggcc ggtgccatca tggcaaaaga actgaccgaa 1080 gccgggctga atgtggtggc gctggagcgt ggtccgcatc gtgacaccta cccggatggc 1140 gcgtatccgc aatccattga tgaactgacc tacaacatcc gtaaaaagct gttccaggac 1200 ctgtcaaaaa gcaccgtcac cattcgtcac gacgcgtcac agacggcagt gccgtatcgt 1260 cagctggcgg cgtttctgcc cggcaccggt accggcggcg cgggcctgca ctggtcaggc 1320 gtacatttcc gtgtcgaccc ggtcgagctg aatctgcgca gccattatga agcgcgttac 1380 ggcaaaaact ttatcccgga aggcatgacg attcaggatt tcggcgtcag ctataacgaa 1440 ctggaaccct tcttcgatca ggcggagaaa gtctttggta cctcgggcag tgcctggacc 1500 atcaaaggca agatgatcgg caaggagaaa ggcggcaact tttacgcgcc ggaccgctcc 1560 agcgacttcc cgctgcccgc acagaagcgg acttactcgg cgcagctgtt tgcccaggcg 1620 gcagagtcgg tgggctatca tccgtacgat atgccatcgg ccaacacctc aggtccgtac 1680 accaacacct acggcgcaca gatgggcccg tgcaacttct gcggctattg cagcggctac 1740 gcctgctaca tgtattccaa agcgtcgcct aacgtgaaca tcctgcccgc gctgcgtcag 1800 gagccgaagt ttgagctgcg taacaacgca tatgtgttgc gcgtcaatct gaccggcgac 1860 aaaaaacgcg ccactggcgt gacctatctc gatggtcagg gtcgtgaagt ggtgcagcct 1920 gcggatctgg tgatcctgtc agcgttccag ttccacaacg tgcacctgat gctgctgtcc 1980 ggtatcggcc agccgtataa cccgatcact aacgaaggtg tggtcggccg taacttcgct 2040 tatcagaaca tctcgacgct gaaagcgctg ttcgacaaaa acaccaccac taacccgttt 2100 atcggtgcgg gtggcgcagg ggtggcggtg gatgatttca acgccgacaa cttcgaccac 2160 ggcccgtacg gcttcgtcgg tggctcgcca ttctgggtga accaggcggg taccaaaccg 2220 gtttccggtc tgccgacccc caaaggcacg ccgaactggg gcagccagtg gaaagcggcg 2280 gtggcggata cctacaacca ccatatttcg atggatgccc acggtgcgca ccagtcatac 2340 cgcgctaact acctcgatct cgatccgaac tacaaaaatg tctacggcca gccgctgctg 2400 cgtatgacct ttgactggca ggacaacgac atcaggatgg cgcagtttat ggtcggcaag 2460 atgcgcaaaa tcaccgaggc catgaatccg aagatgatca tcggcggcgc taagggaccg 2520 ggtacccact tcgataccac cgtgtatcaa accacgcata tgagcggcgg ggcgatcatg 2580 ggtgaagatc cgaaaaccag cgcagtgaac cgttatttgc agagctggga tgtgccgaac 2640 gtgtttgtgc cgggtgcgtc cgcgttcccg cagggtctgg gctacaaccc gaccggcatg 2700 gtggcggcac tgacctactg gtcggcgaaa gccatccgtg aacagtatct gaagaaccca 2760 ggtccactgg tgcaggcata aggaaaacgg cgatgatgaa aagcattctg gccctggttt 2820 tgggcacgct gtcgttcgcc gcgctggcgg acgatcaggc aaatgacgcc ctggtaaaac 2880 ggggtgaata tctggcgcgc gccggtgact gcgtggcctg ccacagcgtc aaaggtgggc 2940 agccttttgc cggtgggttg ccgatggcga cgccgattgg caccatttat tccaccaaca 3000 tcaccccgga taaaaccacc gggattggtg actatagcta cgacgacttc cagaaagcgg 3060 tgcgtcatgg cgtggcgaaa aacggtgaca cgctgtatcc ggcgatgccg tatccgtctt 3120 acgcagtggt gagcgacgag gacatgaagg cgctgtacgc gtactttatg cacggcgtgg 3180 ccccggtggc gcaggctaac aaagacagcg acattccgtg gccgctgtcg atgcgctggc 3240 ctttagctat ctggcgcggc gtgtttgcgc cggacgtgaa agcgttccag cctgccgccc 3300 aggaagatcc ggtgctggca cggggtcgtt atctggtgga aggtctgggt cactgtggcg 3360 cctgccatac gccgcgcagc atcaccatgc aggagaaagc gctcagcaat gatggcgcgc 3420 atgattatct ctccggcagc agcgcaccga ttgatggctg gaccgcaagc aacctgcgtg 3480 gtgacaaccg cgacggcctg ggacgctgga gcgaggacga tctgcgccag ttcctgcgct 3540 atggccgcaa cgatcacacc gccgcgtttg gtggtatgac tgatgtggtg gagcacagcc 3600 tgcaacacct gagcgatgac gatatcacgg caattgcccg ttatctgaag tcgctggggg 3660 cgaaggacgc cagccagacg gtgtttaccc aggatgacca ggtggcgaaa gcgttgtgga 3720 aaggtgatga cagccagact ggcgcgtcgg tgtatgtcga cagctgtgcg gcctgccata 3780 aaaccgacgg cagcaggtta tcagcgcttc ttcccggcgc tgcgtggcaa cccggtggtg 3840 ctggcgaacc cgatccgacg tcgctgatcc acatcgtgct gactggcgga acgctgccag 3900 gcgtgcaggg tgcaccgacg gcgatcacca tgccggcatt cggctggcgc ctgaatgacc 3960 agcaggtggc ggatgttgtg aactttattc gcggcagctg gggcaacggt gccaaagcca 4020 cggtgacggc gaaagatgtc gcatccttac gtaaggatga aaccgtgcag gcgcaccagg 4080 gtaatgcgga tattaaggtg ctggagcaac agcagtaata ttacgtttgc cacgagggga 4140 tttcgttcgc ctcggagtga tttcgttcgc tatgggcact ggcagtttca gctcgccagt 4200 gcggcgaccg agcaaagggg acctggccgt cccctttgca ttccccggcc ttgcgccgcc 4260 ttcctcgccg cttcgcggct ttttcgcgcg ataaatcgcg ccgctacacg ccgcctttcg 4320 ccgcatcctt gcggctcatc ctggaatcgc tcccgcgctc agcgagtccg gatggcgctc 4380 acacccccgc tgcaaccgcg atgacggtct ttggtttttc ttttgttgtt tgtttttatg 4440 agatggtctt gcagacggcg gtgttggcgg cattcgcagc gccgagtgca gaaggaaggc 4500 caggacgagt cgcatggatg cgacgagagc gcggcatggc gcggattgca aaggtccgcg 4560 ccctcggacc tttgcccgtc cgcctgcaca ggcggccctg aaactgccta aagcctggcg 4620 ggcggaaccc ctgcggagct aaaccggtgc cagcgattaa atatt 4665 // |
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