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EBI DbfetchID U96622; SV 1; linear; genomic DNA; STD; PRO; 1740 BP. XX AC U96622; XX DT 27-AUG-1997 (Rel. 52, Created) DT 27-AUG-1997 (Rel. 52, Last updated, Version 1) XX DE Pyrococcus furiosus alpha-amylase gene, complete cds. XX KW . XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-1740 RX DOI; 10.1074/jbc.272.26.16335 RX PUBMED; 9195939. RA Jorgensen S., Vorgias C.E., Antranikian G.; RT "Cloning, sequencing, characterization, and expression of an extracellular RT alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in RT Escherichia coli and Bacillus subtilis"; RL J. Biol. Chem. 272(26):16335-16342(1997). XX RN [2] RP 1-1740 RA Joergensen S.T., Vorgias C.E., Antranikian G.; RT ; RL Submitted (07-APR-1997) to the EMBL/GenBank/DDBJ databases. RL Bacterial Gene Technology, Enzyme Research, Novo Nordisk A/S, Novo Alle, RL Bagsvaerd 2880, Denmark XX FH Key Location/Qualifiers FH FT source 1..1740 FT /organism="Pyrococcus furiosus" FT /strain="DSM3638" FT /mol_type="genomic DNA" FT /db_xref="taxon:2261" FT CDS 229..1611 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-amylase" FT /db_xref="GOA:O08452" FT /db_xref="InterPro:IPR013781" FT /db_xref="PDB:1MWO" FT /db_xref="UniProtKB/TrEMBL:O08452" FT /protein_id="AAB67705.1" FT /translation="MNIKKLTPLLTLLLFFIVLASPVSAAKYLELEEGGVIMQAFYWDV FT PGGGIWWDHIRSKIPEWYEAGISAIWLPPPSKGMSGGYSMGYDPYDYFDLGEYYQKGTV FT ETRFGSKEELVRLIQTAHAYGIKVIADVVINHRAGGDLEWNPFVGDYTWTDFSKVASGK FT YTANYLDFHPNELHCCDEGTFGGFPDICHHKEWDQYWLWKSNESYAAYLRSIGFDGWRF FT DYVKGYGAWVVRDWLNWWGGWAVGEYWDTNVDALLSWAYESGAKVFDFPLYYKMDEAFD FT NNNIPALVYALQNGQTVVSRDPFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPVIFYR FT DFEEWLNKDKLINLIWIHDHLAGGSTTIVYYDNDELIFVRNGDSRRPGLITYINLSPNW FT VGRWVYVPKFAGACIHEYTGNLGGWVDKRVDSSGWVYLEAPPHDPANGYYGYSVWSYCG FT VG" XX SQ Sequence 1740 BP; 515 A; 302 C; 425 G; 498 T; 0 other; cgctatgtgt gtcgagttgc tgtttacagt attccctgtt aaaagttcat cacctacggt 60 aattatttcc gcgaacattg gttctcccca ggaattgttt ttatcaagag tttattagat 120 tttgacgtgc gttgatgaac atttatgttc acatgatcat aacagaaaaa tttatatgta 180 tcatcaccag tgatacatta tgagactttg gtgtatggag gtgatcacgt gaacataaag 240 aaattaacac ccctcctaac tctattactg ttttttatag tactagcaag tccagtaagt 300 gcagcaaaat acttggagct tgaagaggga ggagttataa tgcaagcatt ctattgggat 360 gttccagggg gaggaatttg gtgggatcat ataagatcga agattcctga atggtatgaa 420 gctggaatct ctgcaatatg gctacctcca ccaagcaagg ggatgagtgg aggatattca 480 atgggctacg atccctatga ttactttgat ctcggcgagt actaccagaa gggaactgta 540 gagacgcgtt ttggatcaaa agaagaacta gtgagattga tacaaactgc ccatgcctat 600 ggaataaagg taatcgccga tgtagttata aaccacaggg ctggtggtga cctagaatgg 660 aaccccttcg ttggagatta cacatggaca gacttttcta aagttgcctc agggaaatat 720 acagctaact atctggactt ccatccaaac gagcttcatt gttgtgacga aggaaccttt 780 ggaggatttc cagatatatg tcatcacaaa gagtgggatc agtactggct atggaagagc 840 aatgagagtt atgctgctta tttaagaagc ataggatttg atggttggag atttgactat 900 gttaagggct atggagcttg ggttgtcaga gactggctta attggtgggg aggttgggca 960 gttggagagt actgggacac aaatgtagat gcactactaa gctgggcata tgagagtggt 1020 gcaaaggtct ttgacttccc gctctactat aaaatggatg aagcatttga caataacaac 1080 attccagcat tagtctatgc cctacaaaac ggacaaactg tagtttcgag agatccattt 1140 aaggcagtaa ctttcgttgc caatcatgac acagatataa tatggaacaa gtatccagca 1200 tatgcgttca tattgacata tgagggacag ccagtaatat tctacaggga ctttgaggaa 1260 tggctgaaca aggataagct aattaacctc atttggatcc atgatcattt ggcaggagga 1320 agcacaacaa ttgtctacta cgacaacgat gagctcatat ttgtgagaaa tggagattct 1380 agaaggcctg ggcttataac ttacattaac ttgagcccta actgggttgg taggtgggta 1440 tacgttccaa agtttgcagg ggcttgtatt catgaataca ctggaaacct aggaggatgg 1500 gtagataaaa gagtagatag tagcggatgg gtatacctag aggcaccacc tcacgatcca 1560 gctaacggct actatgggta ctccgtatgg agttattgtg gtgttgggtg actttttctt 1620 ttttcttttt aacaatggga gaagtgcaaa tactgcgaca attcctgggc cgcatacagg 1680 agtttctgga gcggcttcat tcaatattac tgttttattt ccaattccat acaacgtgac 1740 // |
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