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EBI DbfetchID U94513; SV 1; linear; genomic DNA; STD; PRO; 2708 BP. XX AC U94513; XX DT 13-AUG-1998 (Rel. 56, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Erwinia amylovora disease-specific operon, partial sequence; and hrpW DE operon, complete sequence. XX KW Hrp pathway; hypersensitive response; pectate lyase; KW type III protein secretion. XX OS Erwinia amylovora OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Erwinia. XX RN [1] RP 912-2255 RA Kim J.F., Zumoff C.H., Beer S.V.; RT "HrpW, a new harpin of Erwinia amylovora, is a member of a family of RT pectate lyases"; RL Phytopathology 87:0-0(1997). XX RN [2] RP 1-2708 RX PUBMED; 9748455. RA Kim J.F., Beer S.V.; RT "HrpW of Erwinia amylovora, a new harpin that contains a domain homologous RT to pectate lyases of a distinct class"; RL J. Bacteriol. 180(19):5203-5210(1998). XX RN [3] RP 1-2708 RA Kim J.F., Beer S.V.; RT ; RL Submitted (18-MAR-1997) to the EMBL/GenBank/DDBJ databases. RL Plant Pathology, Cornell University, 334 Plant Science Bldg., Ithaca, NY RL 14853, USA XX FH Key Location/Qualifiers FH FT source 1..2708 FT /organism="Erwinia amylovora" FT /strain="Ea321; ATCC 49947" FT /mol_type="genomic DNA" FT /note="a strain that infects apples, pears, and other FT pomaceous plants" FT /db_xref="taxon:552" FT CDS complement(<1..714) FT /codon_start=1 FT /transl_table=11 FT /gene="dspE" FT /product="DspE" FT /note="Hrp-secreted pathogenicity/avirulence protein FT similar to Pseudomonas syringae AvrE" FT /db_xref="UniProtKB/TrEMBL:Q79B28" FT /protein_id="AAC62315.1" FT /translation="MELKSLGTEHKAAVHTAAHNPVGHGVALQQGSSSSSPQNAAASLA FT AEGKNRGKMPRIHQPSTAADGISAAHQQKKSFSLRGCLGTKKFSRSAPQGQPGTTHSKG FT ATLRDLLARDDGETQHEAAAPDAARLTRSGGVKRRNMDDMAGRPMVKGGSGEDKVPTQQ FT KRHQLNNFGQMRQTMLSKMAHPASANAGDRLQHSPPHIPGSHHEIKEEPVGSTSKATTA FT HADRVEIAQEDDDSEF" FT RBS complement(724..729) FT /gene="dspE" FT promoter complement(755..784) FT /gene="disease-specific operon" FT /note="HrpL-dependent promoter consensus sequence; hrp box" FT promoter 825..854 FT /gene="hrpW operon" FT /note="HrpL-dependent promoter consensus sequence; hrp box" FT RBS 900..905 FT /gene="hrpW" FT CDS 912..2255 FT /codon_start=1 FT /transl_table=11 FT /gene="hrpW" FT /product="HrpW" FT /function="elicit the hypersensitive reaction" FT /note="Hrp-secreted; C-terminal domain similar to class III FT pectate lyases; high glycine and serine content; lacks FT cysteine" FT /db_xref="GOA:O54508" FT /db_xref="HSSP:1EE6" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/TrEMBL:O54508" FT /protein_id="AAC62314.1" FT /translation="MSILTLNNNTSSSPGLFQSGGDNGLGGHNANSALGQQPIDRQTIE FT QMAQLLAELLKSLLSPQSGNAATGAGGNDQTTGVGNAGGLNGRKGTAGTTPQSDSQNML FT SEMGNNGLDQAITPDGQGGGQIGDNPLLKAMLKLIARMMDGQSDQFGQPGTGNNSASSG FT TSSSGGSPFNDLSGGKAPSGNSPSGNYSPVSTFSPPSTPTSPTSPLDFPSSPTKAAGGS FT TPVTDHPDPVGSAGIGAGNSVAFTSAGANQTVLHDTITVKAGQVFDGKGQTFTAGSELG FT DGGQSENQKPLFILEDGASLKNVTMGDDGADGIHLYGDAKIDNLHVTNVGEDAITVKPN FT SAGKKSHVEITNSSFEHASDKILQLNADTNLSVDNVKAKDFGTFVRTNGGQQGNWDLNL FT SHISAEDGKFSFVKSDSEGLNVNTSDISLGDVENHYKVPMSANLKVAE" FT RBS 2242..2247 FT CDS 2252..2584 FT /codon_start=1 FT /transl_table=11 FT /product="potential HrpW-specific chaperone" FT /note="putative; OrfC; high glutamine content" FT /db_xref="UniProtKB/TrEMBL:O87915" FT /protein_id="AAC62316.1" FT /translation="MMMKSAEIPPAESWVDVVNSQQHLSPAQYQAFQKAIDQVQQRLHQ FT VLSRPMPQRQGKFELDSFVDSLHADFLIGDSDRSDSNVGQTAWWITRYLADRLLELQQL FT QQQRGL" FT terminator 2655..2692 FT /gene="hrpW operon" XX SQ Sequence 2708 BP; 603 A; 768 C; 723 G; 614 T; 0 other; gaattcgctg tcgtcatctt cctgagcgat ttccactctg tctgcgtggg ccgttgttgc 60 cttgctggtg gagccaaccg gttcttcctt gatttcgtgg tggctacccg ggatgtgcgg 120 cggtgaatgc tgcaggcgat cgccggcgtt ggctgaagcc gggtgagcca ttttgctcaa 180 catcgtttgg cgcatctggc caaaattgtt cagctgatgc cgtttttgct gcgttggtac 240 cttatcttcg ccgctgccac ctttcaccat tggccgcccg gccatgtcgt ccatattgcg 300 gcgtttgacg ccgcccgaac gggtcaaacg cgccgcatct ggcgcggccg cctcatgctg 360 cgtttcgccg tcgtcccgcg ccagcagatc gcgcaatgtt gcccctttgc tgtgggtggt 420 acctggctgg ccctgcggtg ccgatctgga aaattttttc gtccccaaac agcccctgag 480 actgaaggat ttcttttgct ggtgagcagc gctgatacca tcagccgcag tagatggctg 540 gtgaattctc ggcattttcc cacgattttt gccttctgcc gccaatgatg cagcggcatt 600 ttgcgggctg ctgctgctgc tgccctgctg taaggcaaca ccatgcccca cagggttgtg 660 cgccgctgtg tgtactgccg ccttgtgttc agttcccagt gattttaatt ccatgacccg 720 ttgcccccac cctctgccgt aaacattaga tatttttatg ttgcaattct ctgcaaccgg 780 ttcccaccag gttaaaaaaa agtctgcccc ccctcactgt gcgcggaacc ctgtcaacgc 840 caaacccact caattcaggc agcaaatgct gcacgcttag ggctggttaa cgtcgcaaaa 900 ggggaatatc catgtcaatt cttacgctta acaacaatac ctcgtcctcg ccgggtctgt 960 tccagtccgg gggggacaac gggcttggtg gtcataatgc aaattctgcg ttggggcaac 1020 aacccatcga tcggcaaacc attgagcaaa tggctcaatt attggcggaa ctgttaaagt 1080 cactgctatc gccacaatca ggtaatgcgg caaccggagc cggtggcaat gaccagacta 1140 caggagttgg taacgctggc ggcctgaacg gacgaaaagg cacagcagga accactccgc 1200 agtctgacag tcagaacatg ctgagtgaga tgggcaacaa cgggctggat caggccatca 1260 cgcccgatgg ccagggcggc gggcagatcg gcgataatcc tttactgaaa gccatgctga 1320 agcttattgc acgcatgatg gacggccaaa gcgatcagtt tggccaacct ggtacgggca 1380 acaacagtgc ctcttccggt acttcttcat ctggcggttc cccttttaac gatctatcag 1440 gggggaaggc cccttccggc aactcccctt ccggcaacta ctctcccgtc agtaccttct 1500 cacccccatc cacgccaacg tcccctacct caccgcttga tttcccttct tctcccacca 1560 aagcagccgg gggcagcacg ccggtaaccg atcatcctga ccctgttggt agcgcgggca 1620 tcggggccgg aaattcggtg gccttcacca gcgccggcgc taatcagacg gtgctgcatg 1680 acaccattac cgtgaaagcg ggtcaggtgt ttgatggcaa aggacaaacc ttcaccgccg 1740 gttcagaatt aggcgatggc ggccagtctg aaaaccagaa accgctgttt atactggaag 1800 acggtgccag cctgaaaaac gtcaccatgg gcgacgacgg ggcggatggt attcatcttt 1860 acggtgatgc caaaatagac aatctgcacg tcaccaacgt gggtgaggac gcgattaccg 1920 ttaagccaaa cagcgcgggc aaaaaatccc acgttgaaat cactaacagt tccttcgagc 1980 acgcctctga caagatcctg cagctgaatg ccgatactaa cctgagcgtt gacaacgtga 2040 aggccaaaga ctttggtact tttgtacgca ctaacggcgg tcaacagggt aactgggatc 2100 tgaatctgag ccatatcagc gcagaagacg gtaagttctc gttcgttaaa agcgatagcg 2160 aggggctaaa cgtcaatacc agtgatatct cactgggtga tgttgaaaac cactacaaag 2220 tgccgatgtc cgccaacctg aaggtggctg aatgatgatg aaatccgcag agatacctcc 2280 ggcagaaagc tgggttgatg tggttaacag ccagcaacac ctgtcaccgg cgcagtatca 2340 ggcttttcaa aaggccattg atcaggtgca gcagcggctg catcaggtac ttagccgccc 2400 tatgccacaa cgccagggta agtttgagct ggatagcttt gtcgacagtc tgcatgccga 2460 ctttcttatc ggcgacagcg acaggtcaga cagtaacgtc gggcaaacgg cgtggtggat 2520 cacccgttat ctggcggatc gcctgttgga actccagcag ttacagcaac aacgcgggtt 2580 atagccctta tcggcgtgga tttgctgccc tttgcagcca cgctgcttca gccgaccgac 2640 agcgaagatg ccaataaaaa aacgcagcgg taactcacga tgcgtttttt tactgcctct 2700 gagcaagg 2708 // |
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