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EBI DbfetchID U93354; SV 1; linear; genomic DNA; STD; PRO; 2803 BP. XX AC U93354; XX DT 03-JUN-1997 (Rel. 52, Created) DT 15-DEC-1998 (Rel. 58, Last updated, Version 3) XX DE Thermotoga neapolitana endo-1,4-beta-glucanase A (celA) and DE endo-1,4-beta-glucanase B (celB) genes, complete cds. XX KW . XX OS Thermotoga neapolitana OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. XX RN [1] RP 1-2803 RX PUBMED; 9835561. RA Bok J.D., Yernool D.A., Eveleigh D.E.; RT "Purification, characterization, and molecular analysis of thermostable RT cellulases CelA and CelB from Thermotoga neapolitana"; RL Appl. Environ. Microbiol. 64(12):4774-4781(1998). XX RN [2] RP 1-2803 RA Bok J.D., Eveleigh D.E.; RT ; RL Submitted (13-MAR-1997) to the EMBL/GenBank/DDBJ databases. RL Biochemistry and Microbiology, Cook College, Rutgers University, Lipman RL Drive, New Brunswick, NJ 08903, USA XX FH Key Location/Qualifiers FH FT source 1..2803 FT /organism="Thermotoga neapolitana" FT /mol_type="genomic DNA" FT /db_xref="taxon:2337" FT CDS 1..774 FT /codon_start=1 FT /transl_table=11 FT /gene="celA" FT /product="endo-1,4-beta-glucanase A" FT /function="hydrolysis of carboxymethyl cellulose, beta FT glucan" FT /EC_number="3.2.1.4" FT /note="TNCELA" FT /db_xref="GOA:O08428" FT /db_xref="InterPro:IPR002594" FT /db_xref="UniProtKB/TrEMBL:O08428" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC95059.1" FT /translation="MVELTAPGTADFRWNDMPLSMELNLWNVERYTGTVVMRFDGERLT FT FNGDVEDLSAREPERYILGYPEFYYGYKPWERHAAEGTKLPLVVSSVESFTVELSFEID FT HMPSLPLNFAMETWLTREKYQVEASIGDVEIMVWFYFNELTPGGKKVGEYTVSFELNGE FT HKRGIWELWHAEWNWDYLAFRLKNPVRKGRVRFNVKDFLDVAGEYLSRSTRVKDFDDLY FT FTVWEIGTEFGSPETKSARFGWTFNNFSIDMEVKG" FT RBS 759..765 FT /gene="celB" FT CDS 771..1595 FT /codon_start=1 FT /transl_table=11 FT /gene="celB" FT /product="endo-1,4-beta-glucanase B" FT /function="hydrolysis of carboxymethyl cellulose, beta FT glucan" FT /EC_number="3.2.1.4" FT /note="TNCELB" FT /db_xref="GOA:P96492" FT /db_xref="InterPro:IPR013319" FT /db_xref="UniProtKB/TrEMBL:P96492" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC95060.1" FT /translation="MRLVVSFLLVVSAFLFSAEVVLTDIGATDITFKGFPVTMELNFWN FT VKSYEGETWLKFDGEKVQFYADIYNIVLQNPDSWVHGYPEIYYGYKPWAAHNSGTEILP FT VKVKDLPDFYVTLDYSIWYENDLPINLAMETWITRKPDQTSVSSGDVEIMVWFYNNILM FT PGGQKVDEFTTTIEINGSPVETKWDVYFAPWGWDYLAFRLTTPMKDGRVKFNVKDFVEK FT AAEVIKKHSTRVENFDEMYFCVWEIGTEFGDPNTTAAKFGWTFKDFSVEIGE" XX SQ Sequence 2803 BP; 783 A; 598 C; 708 G; 714 T; 0 other; atggttgaac tgaccgcacc gggaacggca gattttcgct ggaatgacat gccgctttcc 60 atggagctga atctgtggaa cgtagaaaga tacacgggaa cggtggtgat gagattcgac 120 ggtgaaagac tcaccttcaa cggtgatgtt gaagatcttt ctgccagaga gccagaaagg 180 tacatccttg gctatcctga gttttactac ggatacaaac cctgggaaag acatgcagca 240 gaggggacaa agttacctct ggtagtgtct tctgtggagt ccttcacggt cgaactttcc 300 ttcgaaatag accacatgcc ctctcttccg ctgaactttg ccatggaaac ctggctcaca 360 agagaaaaat accaggtcga agcttccatc ggagatgtgg agatcatggt gtggttctat 420 ttcaacgaac tcacaccggg tggaaagaaa gtgggagagt acaccgtttc gttcgaactt 480 aacggagaac acaaacgtgg aatctgggaa ctctggcacg ccgaatggaa ctgggattat 540 cttgctttcc ggctgaagaa tcctgtgaga aaggggaggg tgagattcaa cgtgaaggat 600 tttctcgatg ttgcaggaga gtatctttca aggtccacac gtgtgaagga ttttgatgat 660 ctgtatttca ccgtctggga gatcggaacc gaatttggaa gccctgaaac aaagagtgcc 720 cgttttggat ggacgttcaa taacttttca atcgatatgg aggtgaaagg atgaggttgg 780 tcgtctcttt tcttcttgtg gtttctgcct ttcttttctc ggcagaagtc gttctcacag 840 acattggtgc aacagacatt acgttcaaag gctttccggt gaccatggaa ctcaacttct 900 ggaacgtcaa atcctacgaa ggagaaacgt ggctcaagtt cgatggtgaa aaggtccagt 960 tctacgcgga tatctacaac atcgttcttc aaaacccgga cagctgggtt cacggctatc 1020 cagagatata ctacggctac aagccgtggg cggcgcacaa cagcggaaca gagattcttc 1080 cggtgaaggt gaaagatctt cctgactttt acgtaaccct tgattattca atctggtacg 1140 agaacgacct tccgatcaac cttgcaatgg aaacgtggat cacaagaaag ccagatcaga 1200 catctgtttc ctctggagac gtcgagatca tggtctggtt ctacaacaac atcctgatgc 1260 caggtgggca gaaggtggat gaattcacaa caacgatcga gataaacggc tctcctgtgg 1320 aaacaaaatg ggacgtttac tttgcgccct ggggctggga ttatctcgcc ttcaggctca 1380 caactcccat gaaagatggc agggtgaagt tcaacgtgaa ggactttgtg gaaaaagcag 1440 cggaagttat caaaaaacac tcaacaaggg tggaaaactt cgatgagatg tacttctgcg 1500 tctgggagat aggaacagag ttcggtgatc caaataccac cgcggcaaaa tttggatgga 1560 ccttcaagga tttctctgtt gaaattggag aataacgcaa aaactcaccg tgtgaagaag 1620 gcggggcaat cccgcccttt tctttttgga aatttatact caagtagtat aatattctgt 1680 gcagtgtgtg aaaatgagaa caatggggtg gtttcatgaa ggtgaaagac gttctcacgt 1740 tggccataag actggaagaa gagggagaaa ggttttacag ggaactttca gagcatttcg 1800 atggaaagat aaaggaaaca ttccttgaac ttgcagatca ggaaaagctt cacgcggaga 1860 tcttcagaaa gatgtccgat caaaacttct gggacgaggt ggattcttat ctttcgggat 1920 acgccttcta ccaggttttt ccagacaccg atgaaatcct gaaaagaaaa gatctgaccc 1980 tgagggaggt tctggagatc gccatatctg tggaaaaaga cagcataatc ctctacttcg 2040 aactcaaaga cggccttgtg aattctgacg aacagaagac ggtgaagagg atcatcgagc 2100 aagaaaagga acatttaagg aagcttctcg cactgaaacg cgaaagatcc tgaagcgatg 2160 tctttcaggg tgatgcttga aaacatctct tttagatgat ccgacagcca gctccagaag 2220 tactttgccc tgcagtgttt gtaattttca cagcgattgc tgcagtccac aatgacaagg 2280 tctttttcaa aagccctgat gatatctaga aacgaaatct catccgcaga ccttgcaagc 2340 acaaagccgc cgttttttcc tttgatggaa gagacaaggc cagatctttt gagatagtga 2400 aggactttca gggtgaaact tcttgtgatg tgctcttttt ctgcgagttc tgaagaagac 2460 atcggtctgt tttccagaga cagtctcatg aggagcctga gagcgtatct tacatgtttt 2520 tgacactttc caaacatcca aagcacctcc gggagggatc agtgttgaag tacctcatag 2580 ccttgagacc tttttccttt gttgcgagtg cctttcctgt caccgtcgga gccatgctgg 2640 cagaggattt ctccctgacc agatacattc tatcactgat cgctgccgtt ttgcttcatg 2700 ccggtgtcaa caccacgaac gactattttg attacaaaag tggtgtggac acaaaagagt 2760 ctcttggctc aagcgggctt cttgtatcgg gaaagatcac cct 2803 // |
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