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EBI DbfetchID U91484; SV 1; linear; genomic DNA; STD; PRO; 2950 BP. XX AC U91484; XX DT 13-JAN-1998 (Rel. 54, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Rahnella aquatilis positive levansucrase regulator LsrS (lsrS) and DE levansucrase (lsrA) genes, complete cds. XX KW . XX OS Rahnella aquatilis OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Rahnella. XX RN [1] RP 1270-2520 RX DOI; 10.1016/0167-4781(93)90130-6 RX PUBMED; 8318541. RA Song K.B., Joo H.K., Rhee S.K.; RT "Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 RT (ATCC10988)"; RL Biochim. Biophys. Acta 1173(3):320-324(1993). XX RN [2] RP 1-2950 RX DOI; 10.1111/j.1749-6632.1998.tb10369.x RX PUBMED; 9928133. RA Song K.B., Seo J.W., Kim M.G., Rhee S.K.; RT "Levansucrase of Rahnella aquatilis ATCC33071. Gene cloning, expression, RT and levan formation"; RL Ann. N. Y. Acad. Sci. 864:506-511(1998). XX RN [3] RP 1-2950 RX DOI; 10.1016/S0168-1656(00)00281-9 RX PUBMED; 10936661. RA Seo J.W., Song K.B., Jang K.H., Kim C.H., Jung B.H., Rhee S.K.; RT "Molecular cloning of a gene encoding the thermoactive levansucrase from RT Rrahnella aquatilis and its growth phase-dependent expression in RT Eescherichia coli"; RL J. Biotechnol. 81(1):63-72(2000). XX RN [4] RP 1189-2592 RA Seo J.W., Song K.B., Kim C.H., Rhee S.K.; RT ; RL Submitted (27-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Microbial Metabolic Engineering R.U., Korea Research Institute of RL Bioscience and Biotechnology (KRIBB), KIST, P.O. Box 115, Yusong, Taejon RL 305-333, Korea XX RN [5] RC Sequence update by submitter RP 1270-2520 RA Seo J.W., Song K.B., Kim C.H., Rhee S.K.; RT ; RL Submitted (18-JUN-1998) to the EMBL/GenBank/DDBJ databases. RL Microbial Metabolic Engineering R.U., Korea Research Institute of RL Bioscience and Biotechnology (KRIBB), KIST, P.O. Box 115, Yusong, Taejon RL 305-333, Korea XX RN [6] RC Sequence update by submitter RP 1-2950 RA Seo J.W., Song K.B., Kim C.H., Rhee S.K.; RT ; RL Submitted (21-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL Microbial Metabolic Engineering R.U., Korea Research Institute of RL Bioscience and Biotechnology (KRIBB), KIST, P.O. Box 115, Yusong, Taejon RL 305-333, Korea XX CC On Sep 22, 1998 this sequence version replaced gi:3237302. XX FH Key Location/Qualifiers FH FT source 1..2950 FT /organism="Rahnella aquatilis" FT /strain="ATCC 33071" FT /mol_type="genomic DNA" FT /db_xref="taxon:34038" FT gene complement(416..628) FT /gene="lsrS" FT CDS complement(416..628) FT /codon_start=1 FT /transl_table=11 FT /gene="lsrS" FT /product="positive levansucrase regulator LsrS" FT /function="activate transcription of levansucrase gene FT lsrA" FT /note="similar to phage P2 late gene activator Ogr" FT /db_xref="GOA:O87909" FT /db_xref="InterPro:IPR007684" FT /db_xref="UniProtKB/TrEMBL:O87909" FT /protein_id="AAC36459.1" FT /translation="MMRCPLCNHSAHIRTSRYLSQQTKESYYQCQNIECSCTFKTIETV FT SKILCSPTFEKTEGAGCNQEESYLK" FT misc_signal 1021..1217 FT /function="repress transcription of levansucrase gene lsrA" FT /note="in-cis regulatory element lsrR" FT gene 1270..2517 FT /gene="lsrA" FT CDS 1270..2517 FT /codon_start=1 FT /transl_table=11 FT /gene="lsrA" FT /product="levansucrase" FT /EC_number="2.4.1.10" FT /note="beta-2,6 fructan; D-glucose-1-fructosyltransferase; FT extracellular protein" FT /db_xref="GOA:O54435" FT /db_xref="InterPro:IPR003469" FT /db_xref="UniProtKB/Swiss-Prot:O54435" FT /protein_id="AAC36458.1" FT /translation="MTNLNYTPTIWTRADALKVNENDPTTTQPIVDADFPVMSDEVFIW FT DTMPLRSLDGTVVSVDGWSVIFTLTAQRNNNNSEYLDAEGNYDITSDWNNRHGRARICY FT WYSRTGKDWIFGGRVMAEGVSPTSREWAGTPILLNEDGDIDLYYTCVTPGATIAKVRGK FT VLTSEEGVTLAGFNEVKSLFSADGVYYQTESQNPYWNFRDPSPFIDPHDGKLYMVFEGN FT VAGERGSHVIGKQEMGTLPPGHRDVGNARYQAGCIGMAVAKDLSGDEWEILPPLVTAVG FT VNDQTERPHFVFQDGKYYLFTISHKFTYADGLTGPDGVYGFLSDNLTGPYSPMNGSGLV FT LGNPPSQPFQTYSHCVMPNGLVTSFIDNVPTSDGNYRIGGTEAPTVKIVLKGNRSFVER FT VFDYGYIPPMKNIILN" XX SQ Sequence 2950 BP; 861 A; 589 C; 616 G; 884 T; 0 other; atcgattttc ttatcgttat tgcctggtga tgggacagcc agcattaatc taaaaggtct 60 taaaatcttg ctcaaacaat taatagattc tttacttaac tcttacagct aagtactggc 120 gacctgttta ttgcccatca atccataccc gaatttgcca ctggccactg cagctattta 180 tatgaaggct atgtcttatt attaacaatc cgtatattaa ttaagaagta gcaggaattt 240 actgatggat tgccctctcc attcaagcct cgcccccaat caaactaagg gtatttttaa 300 ttctcgctaa aaatgatcaa tagaatgcgc ctcaaacaaa ttaaagacct tagttttttc 360 atttgtttaa taaaagcgca gaatatgaca aacaatatta atctgtggtc gctagctatt 420 ttaaatagga ttcttcctga ttacagcctg caccttctgt tttttcaaag gttgggctgc 480 agagaatttt agaaacggtt tcaattgttt taaaagtgca tgaacactct atgttttggc 540 attgataata tgactccttg gtttgctgtg acaggtatcg actggttcgt atatgtgctg 600 agtgattgca aagcgggcaa cgcatcatag gtaaagcctc gttaatgtaa tcatgctaac 660 tcattgaatg ttaataatta tattgcgtgg ggtaatgatt gtcttacttc gactgagagc 720 gcagaagaaa tgggtgggat gcaaaccaaa ataaagagga gaaaataaaa tcattcctga 780 cataacgctt tccttaaaga tgttgtgtac ttactgtctg atacattcca taaaaattaa 840 ataatttata gctgtcttaa ccctcacaca acaaattaaa ttgacaatga aaagtaattc 900 aaaatatttc ttcctaagga tttattaata attatttaaa attccgcgct attcctcgca 960 gttaaaccga ttatttttct ctgaagattc ataatgagta agcccgtcca ggttgctagc 1020 aattttacta gaatttgtga tgaaccggta gaggataaaa ttcgcttctt ttcctgacga 1080 tgagaaaata atatgtagtc aactacacaa cctccattgc caaaacatat atataaggta 1140 tttcatacaa attatgtata cataatggca caacatcaac tgagtgcatg ccttatttaa 1200 tcatgtaact attcttacga cttcaaacgt caaacagtct taacacttat ttaattaaag 1260 gatatttaca tgacaaattt aaattataca ccgacaatct ggacacgtgc tgatgctctg 1320 aaggtcaacg aaaacgaccc gaccaccacc cagcctattg ttgacgccga ttttccggtt 1380 atgagtgatg aagtatttat ttgggatact atgcccctgc ggtcattaga cggtacggtg 1440 gtttctgtag acggatggtc cgttattttc acgcttaccg ctcagcgtaa taacaataat 1500 tcagagtatc tcgacgcaga aggaaattat gacatcacca gtgactggaa taatcgccac 1560 gggcgcgcaa gaatttgtta ctggtattcc cgtacgggta aagattggat ttttggcggg 1620 cgtgtgatgg cagaaggtgt ctcccctaca tcccgcgaat gggcagggac tcccattttg 1680 cttaatgaag acggtgatat tgatctttat tatacctgcg tcacgccggg agcgactatt 1740 gcaaaagtga gaggcaaagt gctgacctca gaagaaggtg taacgctggc cggtttcaac 1800 gaggttaaat cattattttc agctgacggc gtgtactacc aaactgaaag tcaaaatccg 1860 tactggaact tcagggatcc aagcccgttt atcgatcctc atgatggcaa gctttacatg 1920 gtatttgaag gaaatgtggc gggtgaacgg ggttctcacg tcattggcaa acaggaaatg 1980 ggtaccctgc ccccgggcca tcgtgatgtg ggcaatgcga gataccaggc gggttgtatt 2040 ggtatggccg tcgccaaaga tctttcagga gacgaatggg aaatcctccc acctctggtc 2100 acggctgtcg gtgtgaatga tcaaaccgaa cgcccgcatt ttgtcttcca ggatggaaaa 2160 tattacttat tcactatcag tcataaattt acttatgcag atggtctgac aggtcctgac 2220 ggtgtttacg gcttcctgag tgataacctt accggcccgt attctccgat gaacggctcc 2280 ggtttggtgt taggcaatcc gccttctcag cccttccaga cctactcaca ttgcgtcatg 2340 cccaatggcc tggtgacatc ctttattgat aacgttccga caagcgacgg taattatcgt 2400 attggtggga cagaagctcc taccgtaaaa attgtcctta agggaaaccg ttcctttgta 2460 gagcgcgtgt ttgattacgg gtatatcccg ccgatgaaaa acattatttt aaattaatga 2520 ggtaatctgc ccttccgcat attgtttgcg gaggggcagg ttaaaggagt catagtatct 2580 gaaagatata tgcctcatca gacctcaaag gtaaaaaggc tttattttga aacccgacca 2640 tccattaata atgttgttct taacgctttt ttttcgccag gattctcctg ccagcggtgt 2700 aatgatgtca atgttaatgg cactgctcag gacaatatat ttgaaaggct cccgacggga 2760 aatgatatgc gaagcgttac tttgtggagc tctcactatg actgcgattt cagtaggatt 2820 atactttggg gcctctaaaa atatgatgat aagtatcggc ggtatcattg gttttatcgg 2880 cgctcagaaa aatatgggaa tatttaacca gttatattca gaaaaaatta ttagggaagt 2940 aaacatcgat 2950 // |
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