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EBI DbfetchID U89998; SV 1; linear; genomic DNA; STD; PRO; 6045 BP. XX AC U89998; XX DT 25-AUG-1997 (Rel. 52, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Lactococcus lactis cremoris 50S ribosomal protein subunit L32 (rpmF), 50S DE ribosomal protein subunit L33 (rpmG), 5-methyl-cytosine-methyltransferase DE (scrFIBM), and ScrFI restriction endonuclease (scrFIR), 5-methyl-cytosine DE methyltransferase (scrFIAM) genes, complete cds. XX KW . XX OS Lactococcus lactis subsp. cremoris OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus. XX RN [1] RP 1-6045 RX PUBMED; 8481004. RA Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F.; RT "ScrFI restriction-modification system of Lactococcus lactis subsp. RT cremoris UC503: cloning and characterization of two ScrFI methylase genes"; RL Appl. Environ. Microbiol. 59(3):777-785(1993). XX RN [2] RP 1351-2697 RA Twomey D.P., Davis R., Daly C., Fitzgerald G.F.; RT "Sequence of the gene encoding a second ScrFI m5C methyltransferase of RT Lactococcus lactis"; RL Gene 136:205-209(1993). XX RN [3] RP 1-6045 RX PUBMED; 9245816. RA Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F.; RT "Molecular characterization of the restriction endonuclease gene (scrFIR) RT associated with the ScrFI restriction/modification system from Lactococcus RT lactis subsp. cremoris UC503"; RL Microbiology (Reading, Engl.) 143(Pt):0-0(0). XX RN [4] RP 1-6045 RA Twomey D.P., Davis R., Daly C., Fitzgerald G.F.; RT ; RL Submitted (18-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Dept. of Microbiology., University College Cork, Western Road, Cork, RL Ireland XX FH Key Location/Qualifiers FH FT source 1..6045 FT /organism="Lactococcus lactis subsp. cremoris" FT /sub_species="cremoris" FT /strain="UC503" FT /mol_type="genomic DNA" FT /db_xref="taxon:1359" FT RBS 499..503 FT /gene="rpmF" FT gene 499..688 FT /gene="rpmF" FT CDS 512..688 FT /codon_start=1 FT /transl_table=11 FT /gene="rpmF" FT /product="50S ribosomal protein subunit L32" FT /note="belongs to the L32P family of ribosomal proteins" FT /db_xref="GOA:O34101" FT /db_xref="InterPro:IPR002677" FT /db_xref="UniProtKB/Swiss-Prot:O34101" FT /protein_id="AAB66691.1" FT /translation="MAVPARHTSSAKKNRRRTHYKLTAPTVTFDETTGDYRHSHRVSLK FT GYYKGRKVRDDTK" FT RBS 704..710 FT /gene="rpmG" FT gene 704..867 FT /gene="rpmG" FT CDS 718..867 FT /codon_start=1 FT /transl_table=11 FT /gene="rpmG" FT /product="50S ribosomal protein subunit L33" FT /note="belongs to the L33P family of ribosomal proteins" FT /db_xref="GOA:P0A490" FT /db_xref="InterPro:IPR018264" FT /db_xref="UniProtKB/Swiss-Prot:P0A490" FT /protein_id="AAB66692.1" FT /translation="MRVNITLEHKESGERLYLTQKNKRNTPDKLELKKYSKKLRKHVIF FT KEVK" FT repeat_region 1016..1036 FT /rpt_type=INVERTED FT RBS 1054..1057 FT /note="orfX" FT CDS 1065..1532 FT /codon_start=1 FT /transl_table=11 FT /function="unknown" FT /note="orfX" FT /db_xref="GOA:O34103" FT /db_xref="UniProtKB/TrEMBL:O34103" FT /protein_id="AAB66693.1" FT /translation="MTPIMSFWPSIYDKIKNQIKLIEYRRTFPNDCKYAYMYITKPVKA FT IGGIVYFGKKHDLDDWKKQYSNNTIISDRINSYIQSYRYGMEIIGFQKINPITLDELRK FT NVEGFTAPQSYLLLENNKKLSDYVKNNTVKLGSFIENDLSNIFPEHICKRY" FT RBS 1523..1527 FT /gene="scrFIBM" FT /citation=[2] FT gene 1523..2618 FT /gene="scrFIBM" FT CDS 1536..2618 FT /codon_start=1 FT /transl_table=11 FT /gene="scrFIBM" FT /product="5-methyl-cytosine-methyltransferase" FT /function="catalyses the transfer of methyl group to the FT 5-carbon position of cytosine" FT /function="protects against ScrFI (CCNGG) digestion by FT modifying the second cytosine in 5'-CCNGG-3'" FT /note="methyltransferase variable region very different to FT other 5-methyl-cytosine-methyltransferases that modify FT 5'-CCNGG-3' or a subset of this sequence; M.ScrFIB" FT /db_xref="GOA:P34878" FT /db_xref="HSSP:1FJX" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P34878" FT /citation=[2] FT /protein_id="AAB66694.1" FT /translation="MLRVFEAFAGYGSQRLALIKANIPHEIVGISEIEGDVLLSYSAIH FT ENLLEERNKNIKLTDDEMRDYLKNINIPLDYKTFENRADKLGGQKLKDMYIANKLNKNF FT GDIRSIDPKKLPDFDFFTYSFPCQDISVAGYQNGLVADSGTRSSLLWECCKIIEHKKPK FT YLMMENVKNLVGKNHKVNFNKFLLYLESLGYTNYWDILNARDFGIPQNRERVFCISILN FT PNEDFTFPQKQNLTLSMNDLLEENVSEKFYLKNNQVSDEPILQDYIYCLDSNYWKGTFL FT KDFLSKKRRQLVSGKVRPDGKYPARRLTPRETWRFMGVEDTNFDKASILVSNTSLYKQS FT GNSIVVPVLESLFKELFKSQ" FT RBS 2625..2630 FT /gene="scrFIR" FT gene 2625..3452 FT /gene="scrFIR" FT CDS 2637..3452 FT /codon_start=1 FT /transl_table=11 FT /gene="scrFIR" FT /product="ScrFI restriction endonuclease" FT /function="cleaves 5'-CCNGG-3' sites between the second FT cytosine and central degenerate nucleotide" FT /note="R.ScrFI" FT /db_xref="GOA:O34104" FT /db_xref="InterPro:IPR019063" FT /db_xref="UniProtKB/Swiss-Prot:O34104" FT /protein_id="AAB66695.1" FT /translation="MNPNKERLIEIFRSNVKGRIPDISGRNIRHDGRWGHWLEERFGIS FT ANADNHADILGYELKNEATSGKTTFGDWSANEYIFKTPPYNSLFSGSTASEKQNAFCRM FT FGKPNEAKNGRFSWSGSPIPKIWQYNSFGQIMVIEENLDIVIYYSFSQDLRYNKFEIIP FT PQLQHDEIQIARWYGVANPLLSRRGKTLKDKLEDKFNDLGWFTCTTDSIGAYDKICFGR FT PITFENWINLVDSGIVYFDSGMYEGNKRPYSQWRADNSYWNSLITDCHQ" FT RBS 3604..3611 FT /gene="scrFIAM" FT gene 3604..4786 FT /gene="scrFIAM" FT CDS 3617..4786 FT /codon_start=1 FT /transl_table=11 FT /gene="scrFIAM" FT /product="5-methyl-cytosine methyltransferase" FT /function="catalyses the transfer of a methyl group to the FT 5-carbon postion of cytosine" FT /function="protects against ScrFI (CCNGG) digestion by FT adding a methyl group to the second cytosine of 5'-CCNGG-3' FT sites" FT /note="methyl-transferase variable region is similar to FT other 5-methyl-cytosine methylases that modify the second FT cytosine in 5'-CCNGG-3' or similar sequences; M.ScrFIA" FT /db_xref="GOA:P34877" FT /db_xref="HSSP:6MHT" FT /db_xref="InterPro:IPR001387" FT /db_xref="UniProtKB/Swiss-Prot:P34877" FT /protein_id="AAB66696.1" FT /translation="MTTISRNTGTEISIMIKEKRLRLNMTQKELADAVGMSKNGDRTIR FT RWENGETCPSQLEISAILRFPEIAPFENRKTAKYKMIDLFAGIGGTRLGFHQTEKVKSV FT FSSEIDKFAIKTYKANFGDEPHGDITKIDEKDIPDHDILVGGFPCQAFSQAGKKLGFDD FT TRGTLFFEIARIIKEKRPKAFLLENVKNLKTHDKGRTFKTILNTLEELDYEVHTALFKA FT RDFGLPQNRERIYIVGFDRKSISNYSDFQMPTPLQEKTRVGNILESVVDDKYTISDKLW FT DGHQRRKTENKKNGKGFGYTLFNQDSEYTNTLSARYYKDGSEILIEQKNKNPRKITPRE FT AARLQGFPENFIIPVSDTQAYKEFGNSVAVPTIHAIAEKMLEVLEKSKK" FT stem_loop 4788..4829 XX SQ Sequence 6045 BP; 2136 A; 973 C; 957 G; 1979 T; 0 other; gatcatttca atcaacattg agaaagccaa aactccccca gcaattgtga cgactagacg 60 cgccaaaaaa ggctgattaa agccaaagta agcaatcaat gctacgcctg cgatgacaaa 120 aacaagaatc agttttttcc agtttttcat tcatttctcc ttaaaaattt aattgtccta 180 gaacattgcc ctgctttgtt tggagttttt acgacctgaa tgtcttctcc ttacgccgat 240 ttgattcttt cgaagctgag cttaaatttt taagtaatcc gataagttac ttacatttta 300 tcaaatttat gctaaagtcc ctataaattt agctgtcaaa gatatttgtc agtactttta 360 aaagctgata ttttgctgac agattggtca gtaaaatttg tcaagtaatt ttactttaca 420 aaacttgcat ttctgaaaat ttatgctata ctaataaagt acgtttttgc actctgcaag 480 aacgagataa aattctacgg aggaaaaaac aatggcagta cctgcacgtc acacttcatc 540 agcaaagaaa aaccgtcgtc gtacacatta caaattgaca gctccaactg ttacttttga 600 cgaaactact ggtgactacc gtcactcaca tcgcgtttca cttaaaggct actacaaagg 660 ccgcaaagtt cgcgacgaca ctaaataatt cgacaaaagt tatagaaagg gactatcatg 720 cgcgtaaata ttacacttga acacaaagaa tctggtgaac gtctgtacct tactcaaaag 780 aacaaacgca acactccaga caaattggaa cttaaaaaat actctaaaaa attgcgtaaa 840 cacgtaatct ttaaagaagt taaataaggt ttcattacat gtcaatagac ttgtaaaaca 900 ttgatttaga gcgattgttg aaattttgaa tttcagtata ttgcgttata aatcaacccg 960 atgactacat ttttgactac attcttgaaa ataaaatctt tgggtaaaaa tgatgataac 1020 gctcactgag cgttattttt tagttattga aatggagctt tataatgaca cctataatga 1080 gtttctggcc tagcatttat gacaagatta aaaatcaaat aaaactaatt gaatatagac 1140 ggactttccc taacgactgt aaatatgctt atatgtatat tactaaaccc gttaaagcca 1200 ttggtggtat tgtttatttc ggaaaaaaac atgatttaga cgactggaaa aagcaatatt 1260 caaataatac cattatctct gaccgcatta atagttatat tcagagttat cgttacggaa 1320 tggaaataat tggttttcag aaaatcaatc ctattaccct tgatgagtta cgaaaaaatg 1380 ttgaaggttt cactgcacca caatcatatc ttttacttga aaacaataaa aaattatcag 1440 attatgtgaa gaacaacact gttaaactcg gttcttttat tgaaaatgat ttatcaaata 1500 ttttccccga acatatatgc aagaggtatt aaactatgct aagagtattt gaagcattcg 1560 caggctacgg tagtcaacgt ctagcactaa ttaaagcaaa tattccacat gaaattgtgg 1620 gaatttccga aattgaggga gatgtactgt tatcttattc tgcaattcat gaaaatttac 1680 ttgaggaacg taataaaaac atcaagctaa cagatgatga aatgcgtgac tatctcaaga 1740 acatcaatat tcctttagat tataaaactt ttgaaaatcg agcagataag cttggaggtc 1800 aaaagttaaa agatatgtat atcgcaaata aactcaataa aaattttggt gacatccgct 1860 ctattgaccc aaaaaaatta cctgattttg actttttcac ctattcattt ccatgccaag 1920 atatttcggt tgctggatat caaaatggat tagtagcaga ttctggaacg aggtcttctc 1980 tattatggga atgctgcaaa attattgagc ataaaaaacc taaatattta atgatggaaa 2040 atgttaaaaa tttagtaggt aaaaatcata aagttaactt caataaattt ttattatact 2100 tagaatctct aggatatact aactattggg atattctcaa tgctagggac tttggtatcc 2160 ctcaaaatag agaacgagtt ttttgtatca gtattttaaa tcctaatgaa gattttactt 2220 ttccccaaaa gcaaaattta accttatcta tgaatgattt attagaagaa aatgtttccg 2280 aaaaatttta tctcaaaaat aaccaagtta gcgacgaacc cattcttcaa gattatatat 2340 attgtttaga ttcaaattat tggaagggaa cttttttaaa agatttttta tctaaaaaac 2400 gacgacaatt agtatctgga aaagttcgcc ctgatggaaa atatcctgct agaagactta 2460 ctccacgtga aacatggcgc ttcatgggag tagaggacac taattttgac aaagctagta 2520 ttctcgtaag caatacaagc ctatataaac agtctggaaa tagcattgtt gttcctgtat 2580 tggaatcgct atttaaggaa ctattcaaat cacaatagca ttatggaggt agcaaaatga 2640 acccaaataa agaaagatta atcgaaattt ttagaagtaa tgtaaaagga agaattcctg 2700 atatttcagg aagaaatata cggcatgatg ggagatgggg gcactggtta gaagaacgat 2760 ttggaatatc agccaacgct gacaaccatg cggatattct tggctatgag ttaaaaaatg 2820 aagccacttc tggtaaaact acttttggag attggtctgc aaatgaatat atttttaaaa 2880 cacctcctta taactctctc ttttcaggct ctactgcttc tgagaagcag aatgcttttt 2940 gtcgaatgtt cggaaagcct aacgaagcta aaaatggtcg tttctcatgg tctggttctc 3000 ctattcccaa aatttggcaa tataattctt ttggtcaaat tatggtaatt gaagaaaatt 3060 tggatattgt catttattat tcatttagtc aagatttacg atataataag tttgaaatta 3120 taccacccca acttcaacat gatgaaatcc aaatagcacg ttggtatgga gttgcaaacc 3180 ctctgttaag tcgtagagga aaaactctta aagataaact agaagataaa tttaatgacc 3240 taggttggtt tacatgtaca accgattcaa taggcgcata tgataaaatt tgttttggaa 3300 gaccaatcac atttgaaaac tggattaatc tagtagattc tggcattgta tattttgata 3360 gtggtatgta tgaaggaaac aagcgcccct attctcagtg gagagctgat aatagctact 3420 ggaatagttt aattacggat tgccaccaat aagaaatatg agcatctaat aaaaaattaa 3480 attttggaac gatatttatt cgttcttttt tctttaataa aaataatcct tgcaaatcat 3540 tgaatttatt gctataatta gtgtcaatca gaggacaaaa agtccttata aaaacacttt 3600 atgagaaagg atttatatga ctactatatc aagaaataca ggaacagaaa tttctataat 3660 gattaaagaa aagcgactac gcctaaatat gactcaaaaa gagcttgcag atgcagtggg 3720 catgtcaaaa aacggagata gaaccataag aagatgggaa aatggtgaaa cttgtccatc 3780 acagcttgaa atatctgcta ttttaagatt tccagaaatt gcaccatttg aaaatagaaa 3840 aacagctaaa tataaaatga ttgacctatt tgcaggtatt ggagggacac gcttaggttt 3900 tcatcaaaca gaaaaagtaa agtctgtatt ttcatctgaa atagataaat ttgctattaa 3960 aacttataaa gcgaattttg gagacgaacc tcatggggat ataacaaaaa ttgatgagaa 4020 agatatccca gaccatgata ttcttgtcgg tggatttcct tgtcaagcat ttagtcaagc 4080 aggaaaaaaa ttaggatttg atgatacacg tggaacttta ttttttgaaa tagcaagaat 4140 tataaaagaa aaacgcccta aagcatttct actcgaaaat gtaaaaaatt taaaaaccca 4200 tgataaagga agaactttta aaacaatatt aaatactttg gaagaacttg attatgaagt 4260 tcacacagcg ctattcaaag caagagattt tggtcttcca caaaatagag aaaggattta 4320 tattgtaggt tttgacagaa aatcaattag caactattct gattttcaaa tgcctacacc 4380 tttacaagaa aaaactcgtg ttggaaatat tttagaatcc gttgttgatg ataaatatac 4440 aatatcagat aaactttggg atgggcatca aagaagaaaa actgaaaata aaaaaaatgg 4500 caaaggtttt ggatataccc tctttaatca agatagtgag tatacaaaca ctttatcagc 4560 tcgttattat aaagatggta gtgaaatact aattgaacag aaaaataaaa atcctcgaaa 4620 aattactcct agggaagctg ctcgattaca aggttttcca gaaaatttta ttatacctgt 4680 tagcgatacc caagcttata aagaatttgg aaactctgtt gcagttccca ccattcatgc 4740 tattgcagag aagatgttgg aagttttaga gaagtcaaaa aaataactaa aaaatctcac 4800 ggattaaatc ttatccatga gattttttat attctccaaa tcttcttttc tctgtactat 4860 tacgatatta gacgtatttt gaacagagga atagttgatc tcccttattt cctcatctgt 4920 tagattatat actttgctat caatttgaat catctcacca ttcaatttta tctttacggc 4980 accaatacaa cgcttagggg aaacaggtaa catatagtca gcatcactaa atttttgaag 5040 aataattggc tcatctgata gatataatct ttcttcacct gaaactctaa atattctaag 5100 tcggtaacct tccattatgt gttttgctaa aatttctaaa ccgtttcttt tttcatcttt 5160 tagaaagtgt aatgctagtt ctgactcctg tccttgcaga agactcttag aaagttccac 5220 agccttcatt ttaccaactg tcatttgtcg ataaataata ggcttcataa actcaggaaa 5280 attagaatta taaaatagtt ccttaacttt tggacttcta atcaaagtta ttattaagtg 5340 aaggagcagc tttgcttcca tagtagccca ttgagtgttc ttttttccct taatcatact 5400 ggtatatgtt tttttgaaag tcatctttgc tcataatatc tacaaagtgc ttcatgcttt 5460 tatctacata atcttcaaaa attttatagg aatcttctat ttcatttcta ttgatatact 5520 ctccatactc attttttact tcgtataaat ctatttcttg taagatatct tgtgtctgct 5580 ttttaattat ttccatagtg tttaatttta caacaagaca tttgcccttc tccgtttcaa 5640 aaggacggat agagaattgc ggaacatgat gttgtctttt tacatactga ttttttcttt 5700 ccaatccatc ttgcacctct tttttagtat gctttatcat tttattatct caatatcaaa 5760 ataagatggt aaatgcaaac ctgcgctaaa gtctttatat ttatctccgt tcgcatataa 5820 aacagtcata ttttcaaaat cataaagata ctttgaaagg ttatattcat taggattagt 5880 actatcaagt gataagtcat aaacatcatc tccaattttt ctataggtcg gtagctcaat 5940 tccttttttt aataattttt ctgaatttct ttttattaaa gtgaattttt ggattgccgc 6000 gtgagtaaaa ttatgccctg tcattctttc ttcttcaaat tgatc 6045 // |
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