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EBI DbfetchID U89712; SV 1; linear; genomic DNA; STD; PRO; 6040 BP. XX AC U89712; XX DT 26-MAR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Rhodococcus globerulus hpp operon, 3-(3-hydroxyphenyl) propionate DE hydroxylase (hppA), 2-hydroxy-6-ketonona-2,4-dienoate hydrolase (hppC), DE 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (hppB), putative DE 3-(3-hydroxyphenyl) propionate transport protein (hppK) and putative DE regulatory protein (hppR) genes, complete cds. XX KW . XX OS Rhodococcus globerulus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. XX RN [1] RP 1-6040 RX PUBMED; 9324265. RA Barnes M.R., Duetz W.A., Williams P.A.; RT "A 3-(3-hydroxyphenyl)propionic acid catabolic pathway in Rhodococcus RT globerulus PWD1: cloning and characterization of the hpp operon"; RL J. Bacteriol. 179(19):6145-6153(1997). XX RN [2] RP 1-6040 RA Williams P.A., Barnes M.R.; RT ; RL Submitted (14-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL School of Biological Sciences, University of Wales, Bangor, Memorial RL Building, Deiniol Road, Bangor, Gwynedd LL57 2UW, United Kingdom XX FH Key Location/Qualifiers FH FT source 1..6040 FT /organism="Rhodococcus globerulus" FT /strain="PWD1" FT /mol_type="genomic DNA" FT /note="NCIMB 13325; hpp operon" FT /db_xref="taxon:33008" FT stem_loop 14..54 FT gene 268..1587 FT /gene="hppA" FT CDS 268..1587 FT /codon_start=1 FT /transl_table=11 FT /gene="hppA" FT /product="3-(3-hydroxyphenyl) propionate hydroxylase" FT /note="HppA" FT /db_xref="GOA:O05144" FT /db_xref="HSSP:1FOH" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/TrEMBL:O05144" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAB81312.1" FT /translation="MKTTQDTEVLIVGAGPAGLMLANILGMYGKQVTVLEAMDTLIDYP FT RGVGLDDESFRTIQTVGLVDAIRPHTNPQHIMRLVNGAGKVMLVNNPQTVEFGWERKHG FT FIQPEADKALYEGLSRFGNVQVLFGHRVENVEEDGQSVTAIALVSRPDGTIEERRFSAQ FT YLVGCEGGKSPTRKRLGVSFEGESPSTRWLVVDVNNDPLGTPNVFLGADPKRPYVSIGL FT PHAVRRWEFMLHDDETEEQVTDPDYVNALLADHVPNPAELDFIRRRVFTHHGRVASDFR FT KGRQLIAGDAAHLMPVWMGQGWNSGMRDATNLGWKLAAVLSGQADDALLDTYTSERKDH FT AQAMVDLSLTFGRLIKITNPVGAVLRDAASSVLNLFPQVKSYFADMRFKPMPRYTRGVL FT ADPNTRESGSAAAKLTSKLIPVLTANVKNSPVGVQFRSRV" FT stem_loop 1653..1668 FT stem_loop 1695..1705 FT stem_loop 1933..1941 FT stem_loop 1942..1953 FT gene 2030..2896 FT /gene="hppC" FT CDS 2030..2896 FT /codon_start=1 FT /transl_table=11 FT /gene="hppC" FT /product="2-hydroxy-6-ketonona-2,4-dienoate hydrolase" FT /note="HppC" FT /db_xref="GOA:O05145" FT /db_xref="HSSP:1IUN" FT /db_xref="InterPro:IPR000073" FT /db_xref="UniProtKB/TrEMBL:O05145" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB81313.1" FT /translation="MTTTEIPAYITDDFFGLEDKWIETADGELTHYHELGEGTPILFLH FT GSGTGVTAAANWWLNLPVLSEQGRCIAIDSIGYGQSVVAPNTEYGIKEWVRHAVRVLDA FT LGIEKTWIVGNSLGGWLAFQFAIDFPERLLGIVSMGTGGAKLTGALAGHSNPNLTEAGI FT RKTLELFVVDKSLVTDELVSLRYQSALNDTASDRLAEVVAARDRDRTELPLDFDVLSRL FT DVPVLLIHGVQDVVIPVSRTWELLNVIPNADVHIFSQCGHWSQVERAEEFNTVITQYLS FT ARGVSRS" FT gene 2893..3837 FT /gene="hppB" FT CDS 2893..3837 FT /codon_start=1 FT /transl_table=11 FT /gene="hppB" FT /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase" FT /note="HppB" FT /db_xref="GOA:O05146" FT /db_xref="InterPro:IPR004183" FT /db_xref="UniProtKB/Swiss-Prot:O05146" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB81314.1" FT /translation="MKQALLCMSHSPLLHHLDPPADVKASVEAAFDQARAFVHNFDPDV FT IVNFGPDHYNGFFYDLMPPFCIGYKAKGSGDYDSFAGELNVPEAMAEDLAQFVMDQGLD FT IAISRQMEVDHGAVQPMEIIYGDVASKPLIPVFVNSVARPFVKVARVRKFGEAVGAYFK FT NSDKKVLFIGSGGLSHDPPVPQIATADEAQRKMLTDGRNPTPQARAARQQRVIDTAVKF FT AADEADIMDLNPEWDRGFLDVCASGRIEDFDRYTADDMDAVAGHSSHEVRNWVAAYSAL FT RACGEYEIAYEFYRPIKEYISGFAVTTAILRDI" FT stem_loop 3851..3862 FT gene 3888..5249 FT /gene="hppK" FT CDS 3888..5249 FT /codon_start=1 FT /transl_table=11 FT /gene="hppK" FT /product="putative 3-(3-hydroxyphenyl) propionate transport FT protein" FT /note="contains sugar transport proteins signature 2 FT (PROSITE PS00217); HppK" FT /db_xref="GOA:O05147" FT /db_xref="InterPro:IPR011701" FT /db_xref="UniProtKB/TrEMBL:O05147" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAB81315.1" FT /translation="MDVREAIRTQPMRRYQISTIAICLILSMIDGFEILVMAFVAPHLG FT KSWDISSVEIGYLLSAGIIGTALGAIFISPLADKIGRRRLAIWCLVGITVGMALSACAV FT NVPQLIAFRAFAGLGIGGLVANLNVLVSEFSSDKRRGTALGIYGVGYPLGGAVGGAISG FT VLISVFDWRSAFIFGTIITAIMLVVVIKTLPESIEFLIEKRPVGALERYNEIADRLGYE FT QSSELPVPIRKTERVVRDGLFKGIMLRRTVFLWLGYSCLIASFYFANTWTPKLLSDATG FT DSNMGVTAGVLVNVGGVIGSIIFAGLSIVLRPRVVTVLLMFGGVFAFIGYASGFHIVAL FT ALTLAVLVGIFANGGITGFYAISPPIYPAAARGTGVGWMIGCGRTVSIIAPILTGYLLD FT GGWTPKSVYMMFGGVLVIAGIATLLLDATYRGRSENPETPEARREEDLAAAATA" FT misc_feature 3930..4016 FT /gene="hppK" FT /note="Region: Transmembrane region 1" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4044..4121 FT /gene="hppK" FT /note="Region: Transmembrane region 2" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4131..4202 FT /gene="hppK" FT /note="Region: Transmembrane region 3" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4203..4256 FT /gene="hppK" FT /note="Region: Transmembrane region 4" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4302..4376 FT /gene="hppK" FT /note="Region: Transmembrane region 5" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4392..4469 FT /gene="hppK" FT /note="Region: Transmembrane region 6" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4626..4709 FT /gene="hppK" FT /note="Region: Transmembrane region 7" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4752..4820 FT /gene="hppK" FT /note="Region: Transmembrane region 8" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4830..4895 FT /gene="hppK" FT /note="Region: Transmembrane region 9" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 4902..4970 FT /gene="hppK" FT /note="Region: Transmembrane region 10" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 5028..5114 FT /gene="hppK" FT /note="Region: Transmembrane region 11" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 5112..5174 FT /gene="hppK" FT /note="Region: Transmembrane region 12" FT /inference="non-experimental evidence, no additional FT details recorded" FT gene 5252..5872 FT /gene="hppR" FT CDS 5252..5872 FT /codon_start=1 FT /transl_table=11 FT /gene="hppR" FT /product="putative regulatory protein" FT /note="similar to IclR type regulators; HppR" FT /db_xref="GOA:O05148" FT /db_xref="InterPro:IPR014757" FT /db_xref="UniProtKB/TrEMBL:O05148" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAB81316.1" FT /translation="MCRSGVKPRENRVKGVASESNGSSGKQPGSQTLARGLQALELVAT FT TPDGMTIQEVADALGVHRTIASRLLTTVADFRLIKRGADGKYRAGGGLAALARDLYAGL FT RDEATPLLRRLANSLGASVALFVAEGSEAVAVAVVEPKNARYWVSFREGGRHPIDRGAA FT GYALLAGQPPVPGESSRVTDARRDGYVVSYGEVEPGYWGLGYR" XX SQ Sequence 6040 BP; 1122 A; 1799 C; 1862 G; 1257 T; 0 other; agaggatctg cgcaatggcc gcgccggagg tgaacaacct ccggcgcggc cattttgtgt 60 ctcgggcaca tggagaaggc cggcaaacca tgctctagct gcagttttga tgccgcgcca 120 ttctgtctga gcgatcccat cgggcaccaa atgcggcccc ggtcacaaaa gctcattgac 180 gtaccccgct tcgtggtttc taatagacgc atcgtgcata tagtgtgcta aggcacgcga 240 tagttgcatt agcgcagata ggaagccatg aaaaccactc aggacaccga agtactcatc 300 gtcggcgccg gtcccgccgg cctgatgttg gcgaacatcc tcggcatgta cggcaagcag 360 gtcaccgtgc tcgaggccat ggatacgctg atcgactacc cgcgtggtgt gggactcgac 420 gacgagtcgt tccgcaccat tcagacggtg ggcctggtgg atgcgatccg tccgcacaca 480 aatccgcagc acatcatgcg tctggtcaac ggtgccggaa aggtcatgct ggtcaacaac 540 ccgcagaccg tggaattcgg ctgggaacgt aagcacggct tcatccagcc ggaagccgac 600 aaggcgctgt atgagggact gtcccggttc gggaacgttc aggtgttgtt cggtcatcgc 660 gtcgagaacg tcgaagaaga tggccagtcg gttaccgcta tcgcgttggt ttctcgcccc 720 gacggaacca tcgaagaacg gcgattcagt gcgcagtacc tggtggggtg tgaaggtggt 780 aagtctccga cccgcaagcg tctcggtgtc agcttcgagg gtgaatcacc ttctacacgt 840 tggcttgtcg tggacgtcaa caacgatccg ctgggaaccc ccaacgtgtt cctgggtgcc 900 gatccgaagc gtccgtacgt gtccatcggt ctgccccacg cggttcggcg ctgggaattc 960 atgctgcacg acgacgaaac cgaagagcag gtcacggacc ccgactacgt gaatgctctt 1020 ctggcagatc atgttcccaa cccggcggaa ctcgatttca ttcgacgacg cgtcttcacc 1080 catcacggcc gcgtcgcttc cgacttccgc aagggccgcc aattgatcgc gggcgacgcc 1140 gcgcacctga tgccggtgtg gatgggtcag ggctggaatt cgggtatgcg cgacgcaacc 1200 aatcttgggt ggaagttggc cgccgtactt tccggtcagg cggacgacgc actgctcgat 1260 acctatacgt cggagcgcaa ggatcacgcc caggcaatgg tggatctgtc gctgacgttc 1320 ggacggctca tcaagatcac caatccggtc ggtgctgtac tccgcgacgc tgcgtcgtcg 1380 gtgctcaatc tcttccctca ggtcaagagc tacttcgcgg acatgcgctt caagccgatg 1440 ccgcgctaca ccaggggagt gctggccgac ccgaacactc gggaatcggg aagtgcagca 1500 gcgaaactca cgagcaagct catcccggtg ctgaccgcca acgtcaagaa ctcaccggtt 1560 ggcgtccagt tccgcagccg cgtgtgaact cgcgggatgc gtcggaccaa ctgctcgacg 1620 acgcgatcgg caactggtgg tcggtaatcg tgtggggcaa cagccccaag gacgtgctcc 1680 cacaggattc gctcgacaag ttgtctctac tcggtgcccg ccttgttgcg gtagttccgg 1740 agacccagcg cgaatgggct cagaagcaca tggatccgga cgttatggtc ctcggcgatc 1800 acaccggccg actcaagaag tggttcgacg accgtcccac tcccttggtc ttccttcgcc 1860 cggaccgttt tgttgccggc gcctgcctga cgcagcatgc cccggcgacg ttggacgcga 1920 tcctggccgc aatgaagttc acgccggccg gcgctgtcgc ggacgcagtc gcagtgccgg 1980 tcgcgctcta accatccgcc acacacactc ttacgagaaa gccagatata tgactacaac 2040 cgagattccc gcgtacatca ccgacgattt cttcggcctc gaagacaagt ggatcgagac 2100 cgcagacggt gaactcaccc actaccacga actgggcgaa ggtaccccga tcctgttcct 2160 gcacggttcg ggcaccggcg tcaccgccgc cgccaactgg tggctcaatc ttccggttct 2220 cagcgagcag ggtcgatgca tcgcaatcga ttccatcggc tacgggcaga gcgttgttgc 2280 tccgaacacc gagtacggca tcaaggaatg ggttcgccac gcggtgcgcg ttctcgacgc 2340 cctcggcatc gagaagacgt ggattgtcgg aaactccctc ggtggttggc ttgcattcca 2400 gtttgccatc gatttcccgg aacgccttct ggggatcgtc tcgatgggca cgggcggcgc 2460 aaagttgacg ggcgccctgg ccggtcactc caacccgaac ctgactgaag ccggcattcg 2520 caagaccctc gaactctttg ttgtcgacaa gtcgcttgtc actgacgaat tggtgtccct 2580 gcgctaccaa tccgcgctca acgatacggc ctcggatcgc ctcgccgagg tggtcgcagc 2640 ccgagaccgt gaccgcaccg aactaccgct cgatttcgat gtgctctctc gtttggatgt 2700 tccggttctg ctgatccacg gtgtccagga cgtggtgatc cccgtctcgc gtacgtggga 2760 gttactcaac gtcatcccga atgccgacgt gcatatcttc agccagtgtg gccactggtc 2820 acaggtcgag cgggcagaag agttcaatac cgttatcacg cagtatcttt ccgctcgggg 2880 tgtgagtcgg tcatgaagca ggctttgctg tgcatgtcgc acagcccact gttgcaccac 2940 ctcgacccgc cggccgatgt caaagcatcg gtggaagcag cgttcgacca ggcccgcgca 3000 tttgttcaca atttcgatcc cgatgtgatc gttaacttcg gacccgatca ctacaacgga 3060 ttcttctacg atctgatgcc gccgttctgc atcggctaca aggcaaaggg cagtggcgat 3120 tacgactcgt ttgccggtga actgaacgtg ccggaagcta tggccgagga cttggcgcag 3180 ttcgtgatgg atcaggggct cgacattgcg atttcacgtc agatggaggt cgatcacggt 3240 gcggtgcaac cgatggagat catctacgga gacgtagcgt ccaaaccgct gatcccagtc 3300 ttcgtcaatt cggttgccag gccgttcgtg aaggttgccc gcgtgcgtaa gttcggcgag 3360 gccgtcggcg cgtatttcaa gaattccgac aagaaggtgc tgttcatcgg ttccggtggg 3420 ctgtcacatg atccgccggt tccgcagatc gccaccgccg acgaggctca gcgcaagatg 3480 ctgaccgacg gccggaatcc gacgccgcag gcgcgtgcgg ccaggcaaca gcgagtcatc 3540 gacacggccg tcaagttcgc ggcggacgag gccgacatca tggatctgaa tccggagtgg 3600 gacagaggat ttctcgacgt gtgcgcgtcc gggcggatcg aggatttcga tcgctacacc 3660 gcagacgaca tggatgcggt agccgggcac tcctcgcatg aggtgcgcaa ctgggtggct 3720 gcctactctg ccctacgggc ctgcggtgaa tacgagatcg cctacgagtt ctaccggccg 3780 atcaaggaat acatctccgg ctttgccgtc acgaccgcga ttctgcggga tatctgattc 3840 ttgctactcc ggggaagccc cctcccatct tatgaaagta gacctcgatg gatgttcgtg 3900 aagcgattcg gacgcaaccg atgcgccgat atcagatttc gacaatcgcg atctgtctga 3960 tcctcagcat gatcgacggt ttcgagatcc tggtcatggc gttcgtagcg cctcacctcg 4020 gaaagtcctg ggacatcagc tccgtcgaga tcggctacct gctcagtgcc ggcatcatcg 4080 gcacggctct gggtgcaatt ttcatctccc cattggcgga caagatcggt aggcgccggc 4140 ttgcgatctg gtgtctcgtc ggtatcaccg tcggaatggc gttgtctgcc tgcgccgtca 4200 acgttcccca gctcatcgca ttccgtgcgt ttgccggctt ggggatcggc ggactggtcg 4260 cgaacctgaa tgttcttgta tcggagttca gttcggacaa gcgtcgtggt accgccctgg 4320 gaatctatgg tgtcggctac cccctcggtg gagccgtggg tggcgcgatt tccggcgtcc 4380 tgatttccgt gttcgactgg cggtcggcgt tcatcttcgg cacgatcatc accgcaatca 4440 tgctggttgt cgtgatcaag accctgccgg aatccatcga gttcctcatc gagaagcggc 4500 cggtcggggc actggagcgg tacaacgaga tcgcggatcg gctcggctac gagcagtcgt 4560 cggaactgcc cgttccgatt cgaaagaccg aacgtgttgt gcgggacggc cttttcaagg 4620 gaatcatgct gcgtcgcacg gttttcctct ggctcgggta ctcatgcttg atcgcgtcgt 4680 tctacttcgc caatacctgg acgccgaagt tgctgtccga tgcaacgggg gattcgaaca 4740 tgggcgtcac tgccggtgtg ctcgtcaacg tcggtggtgt catcggatcg atcatttttg 4800 cgggcctgtc gatcgtgttg cggccgagag ttgtgacagt tctcttgatg ttcggtggag 4860 tatttgcgtt catcggttac gcctccggat tccacatcgt cgccctggct ttgacgctcg 4920 cggtactggt cggcattttt gccaatggcg gcatcaccgg tttctacgcg atcagcccgc 4980 ccatctaccc ggcggcggcc cggggcaccg gtgtgggctg gatgatcggg tgcggtcgca 5040 cggtctcgat tatcgctccg attctgaccg gctacctgct cgatggtggc tggactccga 5100 agagcgtcta catgatgttc ggtggggtgt tggtgatcgc cggaatcgcg acgttgcttc 5160 tcgacgcgac ctaccgcggc cgcagtgaga atccggagac tccggaggcg agacgggaag 5220 aggacctggc cgccgcagcc actgcttgag gatgtgccga tccggcgtca agccccgaga 5280 gaatagagtc aagggcgtgg cttcagaatc gaatgggtcg tccggcaaac aaccgggctc 5340 gcaaacactg gcgaggggat tgcaggccct cgaattggtt gccaccactc ccgatggaat 5400 gacaattcag gaagtagccg atgccctggg tgttcaccgg accattgcat cgcggttgct 5460 caccactgtg gctgacttcc ggctcatcaa gcgcggtgcg gacggcaagt accgggccgg 5520 gggcggattg gcggcgctcg ctcgggactt gtacgcaggc cttcgagacg aggcgacacc 5580 gttgttgcgt cggctcgcga attccttggg cgcgagtgtt gcgctgtttg tcgcggaggg 5640 ctccgaagcg gtggccgtcg ccgtcgtcga gccgaagaat gctcggtact gggtgtcgtt 5700 ccgtgagggt ggtcgccacc ctatcgaccg cggcgccgcc ggctatgcgt tgttggccgg 5760 gcagccgccc gtccccgggg aatcgagtcg agtcaccgat gcccgccggg acggctacgt 5820 cgtcagttac ggcgaagtcg agccgggata ctggggcctc gggtaccgtt gacacacggc 5880 caatgagccg gccggttgcc tgacgttgat taccgcgtcc gaggatctgg cccgcggcgc 5940 agtgcgcgaa ttgatggatg ctgctgaaaa gatcagcggc acaacacact agctacaagt 6000 gcggcagtat tcccacgaag aacgatgcgc tcaggcaagc 6040 // |
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