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EBI DbfetchID U89396; SV 1; linear; genomic DNA; STD; PRO; 4509 BP. XX AC U89396; XX DT 18-NOV-1997 (Rel. 53, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Staphylococcus aureus hemCDBL gene cluster: porphobilinogen deaminase DE (hemC), uroporphyrinogen III synthase (hemD), d-aminolevulinic acid DE dehydratase (hemB) and GSA-1-aminotransferase (hemL) genes, complete cds. XX KW . XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-4509 RX PUBMED; 7922886. RA Kafala B., Sasarman A.; RT "Cloning and sequence analysis of the hemB gene of Staphylococcus aureus"; RL Can. J. Microbiol. 40(8):651-657(1994). XX RN [2] RP 1-4509 RX DOI; 10.1016/S0378-1119(97)00372-7 RX PUBMED; 9358061. RA Kafala B., Sasarman A.; RT "Isolation of the Staphylococcus aureus hemCDBL gene cluster coding for RT early steps in heme biosynthesis"; RL Gene 199(1-2):231-239(1997). XX RN [3] RP 1-4509 RA Kafala B., Sasarman A.; RT ; RL Submitted (13-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Department of Microbiology and Immunology, Universite de Montreal, Quebec, RL Canada XX FH Key Location/Qualifiers FH FT source 1..4509 FT /organism="Staphylococcus aureus" FT /strain="RN450" FT /mol_type="genomic DNA" FT /sub_clone="pEB-S1; pEB-S4 (I and II); pEB-S2" FT /db_xref="taxon:1280" FT promoter 139..168 FT /note="hemCDBL gene cluster" FT RBS 205..214 FT /gene="hemC" FT CDS 219..1145 FT /codon_start=1 FT /transl_table=11 FT /gene="hemC" FT /product="porphobilinogen deaminase" FT /EC_number="4.3.1.8" FT /db_xref="GOA:O34090" FT /db_xref="InterPro:IPR000860" FT /db_xref="UniProtKB/Swiss-Prot:O34090" FT /protein_id="AAC45833.1" FT /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGDR FT IVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELPFD FT AYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTEDYD FT AIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDE FT VAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTMNGTDP FT VELGKTVSNKLKEQGAYEIIKRLNEQH" FT RBS 1150..1157 FT /gene="hemD" FT CDS 1167..1835 FT /codon_start=1 FT /transl_table=11 FT /gene="hemD" FT /product="uroporphyrinogen III synthase" FT /EC_number="4.2.1.75" FT /db_xref="GOA:O34091" FT /db_xref="InterPro:IPR003754" FT /db_xref="UniProtKB/TrEMBL:O34091" FT /protein_id="AAC45834.1" FT /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDWL FT IFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSFNQ FT TNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDALTF FT SSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKILESRG FT " FT RBS 1827..1833 FT /gene="hemB" FT CDS 1838..2809 FT /codon_start=1 FT /transl_table=11 FT /gene="hemB" FT /product="d-aminolevulinic acid dehydratase" FT /EC_number="4.2.1.24" FT /db_xref="GOA:Q2FXR3" FT /db_xref="InterPro:IPR001731" FT /db_xref="InterPro:IPR013785" FT /db_xref="UniProtKB/Swiss-Prot:Q2FXR3" FT /protein_id="AAC45835.1" FT /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEI FT KSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATRIA FT KKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGADIIA FT PSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQM FT DPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTK FT AAAQNGWIDEERVVMEQMVSMKRAGADMIITYCKDICRYLDN" FT RBS 2841..2847 FT /gene="hemL" FT CDS 2857..4143 FT /codon_start=1 FT /transl_table=11 FT /gene="hemL" FT /product="GSA-1-aminotransferase" FT /EC_number="5.4.3.8" FT /db_xref="GOA:Q2FXR4" FT /db_xref="InterPro:IPR004639" FT /db_xref="InterPro:IPR005814" FT /db_xref="InterPro:IPR015421" FT /db_xref="InterPro:IPR015424" FT /db_xref="UniProtKB/Swiss-Prot:Q2FXR4" FT /protein_id="AAC45836.1" FT /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSK FT IYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVIDR FT VPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVATLG FT LPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPIEGFL FT QGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGK FT KEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILEDGLKRVF FT AKHNVPITVNRAGSMIGYFLNEGPVTNFETANKSDLKLFAEMYREMAKEGVFLPPSQFE FT GTFLSTAHTKEDIEKTIQAFDTALSRIVK" FT terminator 4162..4184 FT /note="putative; rho-independent" XX SQ Sequence 4509 BP; 1563 A; 632 C; 897 G; 1417 T; 0 other; cgtatattca ttgacccgaa ggtaatactc tcttcaatta ttacagtatt atatggaagt 60 tatattttac ttagaatcaa caaatggttg aattcaagat atttaatata ctataacatt 120 attttatttt gcttaaatat gattaattta ttttttgcaa cacattttgt gaattgagaa 180 aataatctag agcaatccgt tcctaggagg aattgaacat gcgtaaatta gtcgttggct 240 ccagaagaag taaattagct ttaacacaaa gccagcaatt tattgataaa ttaaaagctg 300 tcgagccaaa tctagaaatt gaaattaaag aaattgtcac gaaaggcgat cgtatagtag 360 ataaacaatt gtctaaagtc ggaggcaaag gcttatttgt taaagaaata caacatgaac 420 tttttgaaaa aaatatcgat atggcaatac actcgcttaa agacgtacca agtgtaattc 480 cggaaggttt aacattaggt tgtatccctg atagagaatt accttttgat gcgtatattt 540 ctaaaacaca tacaccacta tcccaattgc cagaaggcag tattattggt actagttcat 600 tacgtcgtgg tgcacaaata ttatctaagt atcctaattt agagattaaa tggattagag 660 gtaatataga tacacgatta gaaaagttac aaactgaaga ttatgatgcg attattttag 720 ctgcagctgg tttaagaaga atgggctggt cagatgatat tgtaacatct tatcttgata 780 gagatacatt gttacctgca atcggacaag gtgctttagg gatagaatgt cgtagtgacg 840 atgaagaact attaacatta ttaagcaaag tacataatga tgaggttgca aaatgtgtga 900 ctgctgaacg aacgttttta gcagaaatgg atggtagttg tcaggtgcca attgcaggat 960 atgctacaat ctcagatcaa aacgaaatcg aatttacagg tttaattatg accccagatg 1020 gtaaagaacg atttgaatat acaatgaacg gaacagatcc ggttgagtta ggcaaaacag 1080 tgagtaacaa attaaaagag caaggtgctt atgaaattat aaaacgctta aatgaacaac 1140 attaaataat ggagtgacgt ttaaatatga agccagttgt agttatgaca caaacaaatg 1200 acatgcaaag tgatttagta tcaattattc acaaaccctt tattgatata aagccactaa 1260 attttgatat tcatttgtta aatcaacgct atgactggct tattttttca tctaaaaatg 1320 ctgtgaaatt cttttataaa tatctaaaag gaattaatgt tgataacatt gctgtgatag 1380 gaagtaagac agcgcaatat tgtgaatcac ttggcattcg agttgatttt atgccaaacg 1440 acttttctca agaaggattt ttaaaatcat ttaatcaaac taaccaaaaa atacttttgc 1500 cttcgagtga attggcgaga ccattgttat tagcagcgtt atctaaagat aatgaagttg 1560 ttaaaataga tttatatact tcagtgccta acaaacaaaa tatacaagat gttaaagaaa 1620 tgatagaaca tcaacaaatc gatgcattaa cattttcaag ttcgtcggca gtacgttatt 1680 attttaatga aggatttgta ccaaaattca agtcgtattt tgctattgga gaacaaacag 1740 cacggaccat taaatcatat caacaaccag taacaattgc agaaattcaa acactcgaat 1800 cactaattga aaagatttta gaaagtaggg gctaaaaatg aaatttgata gacatagaag 1860 attgagatca tcagcgacaa tgagagatat ggttagagag aatcatgtaa gaaaagaaga 1920 tttaatatat ccaatttttg tagttgaaaa agacgatgtg aaaaaagaaa ttaagtcatt 1980 gccaggtgta taccaaatca gtttgaattt acttgaaagt gaattaaaag aagcttatga 2040 cttaggcata cgtgccatta tgtttttcgg tgttccaaac tcaaaagatg atataggtac 2100 tggtgcatac attcacgatg gtgttattca acaggcaaca cgtattgcta aaaaaatgta 2160 tgatgactta ttaattgttg cagacacttg tttatgtgaa tatactgatc atggtcattg 2220 tggcgtgatt gatgaccata cacatgacgt tgacaatgat aaatcattgc cactacttgt 2280 taaaacagca atttctcaag tggaagctgg tgctgatatt attgcgccaa gtaatatgat 2340 ggatggtttt gttgctgaaa ttcgtcgtgg attagatgaa gccggctatt acaatattcc 2400 tataatgagt tatggtgtca agtatgcatc aagtttcttt ggacctttta gagatgcagc 2460 agattcagcg ccatcatttg gggatagaaa aacgtatcag atggaccctg ctaaccgttt 2520 ggaagcactt cgtgaattag aaagtgatct taaagaaggg tgcgacatga tgattgttaa 2580 acctgctcta agttatttag atatagttcg agatgttaaa aatcatacga atgttccagt 2640 tgttgcatat aatgtgagtg gagaatatag tatgactaaa gcagcggcac aaaatggttg 2700 gatagatgaa gaacgtgtcg ttatggaaca aatggtttca atgaaacgtg caggtgctga 2760 tatgattatt acgtattgca aggacatttg tcgctattta gataattaag gttttatatt 2820 tatgattttc cataaactgt aggaggaatt tactttatga gatatacgaa atcagaagaa 2880 gcaatgaagg ttgctgaaac tttaatgcct ggtggtgtaa atagtccagt acgcgcattt 2940 aaatcagtag atacaccagc aatttttatg gatcacggta aaggttcaaa aatttatgat 3000 atcgatggta acgagtatat cgactatgta ctaagttggg gaccacttat tttaggacat 3060 agagaccctc aagttattag tcatttacat gaagcaattg ataaaggtac aagttttggt 3120 gcatcaacat tacttgaaaa taaattggcg cagctcgtta ttgaccgagt accttcaata 3180 gaaaaagtgc gtatggtgtc atctggtaca gaagctacat tggatacttt aagattagca 3240 cgtggttata ctggcagaaa taaaattgtg aaatttgaag gttgctatca tggtcatagt 3300 gattcgttat taatcaaagc tggttctggg gtggcaacat taggattgcc ggattctcct 3360 ggtgtgcctg aaggtattgc taaaaataca attacagttc catacaatga tttagatgca 3420 cttaaaatcg ctttcgaaaa atttggaaac gatattgctg gtgtaatcgt agaacctgtt 3480 gctggtaata tgggtgtcgt cccgccgatt gaaggttttt tacagggatt aagagatatt 3540 acgactgaat acggcgcatt gctaattttc gatgaagtaa tgactggttt cagagtcggt 3600 tatcattgtg cacaaggtta ctttggtgtg acaccagatt taacttgctt aggaaaagtt 3660 atcggtggag gactacctgt aggtgcattt ggtggtaaaa aagaaatcat ggatcatata 3720 gcaccattag gaaatattta tcaagcgggt acgttatcag gaaatcctct tgcaatgaca 3780 agtggttatg aaacgttaag ccaattaacg ccagagacat atgagtattt taatatgtta 3840 ggcgatatac ttgaagacgg tttaaaacgt gtatttgcta aacacaatgt accaataact 3900 gtaaatagag caggttcaat gattggttat ttcttaaatg aaggacctgt aactaatttt 3960 gaaacagcga ataaaagtga tttgaaatta tttgcagaaa tgtatcgaga aatggcaaaa 4020 gaaggtgtgt ttttaccacc atctcaattt gaaggtacat tcttatctac ggcacacacg 4080 aaagaagata ttgaaaaaac gattcaagca tttgatacgg ctttaagtcg tattgtaaaa 4140 taaaatatac ggacaaattg agagcctgaa ctttgttcag gctcttttta aatgtatata 4200 aggcatgggc ggcgacttga tagtgaaagt ccatatatgc tttgtagtca aaactgctag 4260 cggatattgt tatcttaaca aacgtgaagc tcaagcagca atttagtcat tttatttttt 4320 attgaaagaa gtggaaaaca tggcaatgat atatagaaat aatttcattg tgttcgtttt 4380 atcatttttt attagtattc tactgtattc atcgcacgta ttacttccat ttatgtttgg 4440 ttcctattat cgcatcaatc atttgtgtaa aagttttcaa acttgaataa ttaaatggcc 4500 attcttact 4509 // |
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