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EBI DbfetchID U88907; SV 1; linear; genomic DNA; STD; PRO; 3130 BP. XX AC U88907; XX DT 05-MAY-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Pseudomonas wisconsinensis selenium-dependent glutathione peroxidase-like DE protein (gpwA), extracellular lipase (lpwA) and lipase helper protein DE (lpwB) genes, complete cds. XX KW . XX OS Pseudomonas wisconsinensis OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-3130 RA Hazbon M.H., Dufel H., Cornelis P., Jaeger K.E.; RT "Molecular characterization of an operon from Pseudomonas wisconsinensis RT responsible for the production of an extracellular lipase"; RL Unpublished. XX RN [2] RC European Patent application: WO96/12012 RP 1-3130 RA Andre C., Charmoille L., Cornelis P., Hazbon M.H.; RT "Lipase, micro-organisme la produisant, procede de preparation de cette RT lipase et utilisation de celle-ci"; RL Unpublished. XX RN [3] RP 1-3130 RA Hazbon M.H., Dufel H., Cornelis P., Jaeger K.E.; RT ; RL Submitted (07-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Plant Physiology, Vrije Universiteit Brussel, Paardenstraat 65, Sint RL Genesius Rode B-1640, Belgium XX FH Key Location/Qualifiers FH FT source 1..3130 FT /organism="Pseudomonas wisconsinensis" FT /strain="LMG P-15151" FT /mol_type="genomic DNA" FT /note="soil isolate from a Wisconsin air field; classified FT by 16S ribosomal RNA sequencing as a Pseudomonas group I FT strain" FT /db_xref="taxon:57038" FT gene 278..946 FT /gene="gpwA" FT CDS 278..946 FT /codon_start=1 FT /transl_table=11 FT /gene="gpwA" FT /product="selenium-dependent glutathione peroxidase-like FT protein" FT /note="GpwA; not incorporating selenium; periplasmic FT localization; 22 kDa; no GPX activity detected against FT t-butyl H2O2; overexpression not necessary for higher FT lipase expression; GPX-like" FT /db_xref="GOA:O08368" FT /db_xref="InterPro:IPR000889" FT /db_xref="InterPro:IPR012335" FT /db_xref="InterPro:IPR012336" FT /db_xref="UniProtKB/Swiss-Prot:O08368" FT /protein_id="AAB53646.1" FT /translation="MSIRRLACSLLLSSLALPVLAAECPAMLQGELPKLRAKGENIELC FT QYAGKPLVVVNTASFCGFTPQFKGLEALYQRYKDQELEVLGVPSDDFRQESADSKETAT FT VCYVNYGVTFAMTEPQPVSGANAIPLFKGLAEQSRQPRWNFFKYVVDRQGKVVASFSSL FT TKPDDPELIAARREGDRLATLIPSRFTALHPSGRWALSVCLPRLARRRTQFTRRALQEQ FT " FT gene 957..1883 FT /gene="lpwA" FT CDS 957..1883 FT /codon_start=1 FT /transl_table=11 FT /gene="lpwA" FT /product="extracellular lipase" FT /function="glycerol ester hydrolase" FT /EC_number="3.1.1.3" FT /note="LpwA" FT /db_xref="GOA:O08369" FT /db_xref="HSSP:1EX9" FT /db_xref="InterPro:IPR000073" FT /db_xref="UniProtKB/TrEMBL:O08369" FT /protein_id="AAB53647.1" FT /translation="MRRVYTAALATLALLGAVEAQANYTKTKYPIVLVHGVTGFNTIGG FT LVNYFHTIPWNLERDGARVHVASVAAFNDSEQRGAELARQIVPWAAGGGGKVNLIGHSQ FT GSPTSRVAASLRPDLVASVTSINGVNKGSKVADVVRGVLPPGSGIEGGANAIANALGAV FT INLLSGSSNPQNGINALGTLTTAGTSALNSRHPWGVNTSSYCAKSTEVHNVRGHSIRYY FT SWTGNAAYTNVLDAADPFLAFTGLVFGSEKNDGLVGVCSTYLGQVIDDSYNMNHVDAIN FT HLFGIRGWTEPVSLYRQHANRLKNKGV" FT gene 1926..2984 FT /gene="lpwB" FT CDS 1926..2984 FT /codon_start=1 FT /transl_table=11 FT /gene="lpwB" FT /product="lipase helper protein" FT /function="putative foldase" FT /note="LpwB; necessary for activation of LpwA" FT /db_xref="GOA:O05938" FT /db_xref="InterPro:IPR004961" FT /db_xref="UniProtKB/Swiss-Prot:O05938" FT /protein_id="AAB53648.1" FT /translation="MSKFFSLSLVAVVVAGGLTLYWRWPAAVPEAQPATTVAVPSRQSL FT IEQASTSPAKAPAEAQPSIAVTLPSLAGTEVDGQLRTDAAGNLLLDLAVRDYFDYFLSA FT VDHSGLDAVIEALLADAGRRLPEPALGQMISLLGDYLDYKRASMALMQQPLDARQQVEP FT QAQLQALQSAFARLDELRRAHFSATAQEALFGAEQAYARYTLDSLAVQQRDDLGEAQRT FT QLLEQLRERLPDALRESEQRQQLAQEQLQRSEQLWRDGADEQQVREFLAMTYDPDTVQR FT LLDEQRRERDWQQHYQAYRNELASLQGRGLSEADGEQLQRQLRERLFSSEDRHRVETYD FT AIAAKQPEPLDP" XX SQ Sequence 3130 BP; 561 A; 1013 C; 975 G; 581 T; 0 other; ctgcagttgg gcatcggtat ctgctaggtt ctgcacgata agtgcctgcg ctcgttgatg 60 ctgcgttaaa gacgggctgg aacgccagcc cggtcgaatg ctcatttaca actcgtaaac 120 tcgaatgcga gcccagtccg ttcctcaccc gtttttgtgg ggccgccatc ggtatcgcct 180 gaccttcgct cgacgacttt tcgtacagac ccgagttgct tgcgtctggg cctgcgagta 240 gcgtggtgga atcgcgtcat tgcccgaggt cactgccatg tctattcgtc gtcttgcctg 300 ttcacttttg ctctccagcc tggcgttgcc ggtgcttgcc gcagagtgtc cggccatgct 360 ccagggcgaa ctgcccaagc tgcgcgccaa aggtgaaaac atcgagctct gtcagtacgc 420 gggcaagccg ctggtggtgg tcaataccgc cagcttttgc ggcttcaccc cgcaattcaa 480 agggcttgaa gcgctttacc agcggtacaa ggatcaggaa ctggaggtat tgggcgtgcc 540 gtccgacgac ttccgccagg aatccgccga tagcaaggaa acggccaccg tctgctacgt 600 caactacggc gtgaccttcg ccatgaccga accgcagccg gtttccggtg ccaatgccat 660 cccgttgttc aagggcctgg ccgagcagag ccggcagccg cgctggaact tcttcaagta 720 cgtggtcgac cgtcagggca aggtggtggc gagtttctcc agcctgacca agcctgacga 780 tcccgagctg attgccgccc gtagagaagg cgatcgcctc gcaaccctga ttccgtcccg 840 cttcacggcc cttcacccat ctgggcgatg ggccttgtcc gtctgcctgc ctagactggc 900 caggcgccgg acgcagttca cccggcgtgc cctacaagaa caataaggag gcacccatgc 960 gtcgcgtcta taccgctgcc ctggcaacac tcgctctgct tggcgccgtc gaggcccagg 1020 ccaactacac caagaccaag tatccgatcg tgctggtaca cggcgtgacc gggttcaata 1080 ccatcggcgg gctggtcaat tacttccata ccattccctg gaacctagag cgcgatggcg 1140 cccgggtgca cgtcgccagt gtcgctgcct tcaatgacag cgagcagcgc ggcgccgagc 1200 tggcccggca gatcgtgccc tgggccgcag gcggaggcgg caaggtcaac ctgatcggcc 1260 acagtcaggg ctcgccgacc tcgcgcgtgg cggcttcgtt gcggccggat ctggtggcat 1320 cggtgacctc gatcaacggc gtcaacaagg gctccaaggt cgccgatgtg gtgcgcggcg 1380 tgctgccacc gggtagcggt atcgaaggcg gcgccaatgc catcgccaac gccctcggtg 1440 cggtgatcaa tctgctgtct ggctcaagca acccgcaaaa cggtatcaac gcgctaggca 1500 ccctgaccac cgcgggcacc agtgcgctga acagtcgcca cccgtggggc gtcaacacca 1560 gcagctactg cgccaagtcc accgaagtgc acaatgtgcg cggtcacagc atccgctact 1620 actcctggac cggtaatgcc gcctatacca acgtgctcga tgcggccgat cccttcctgg 1680 ccttcaccgg cctggtgttc ggcagcgaga agaacgacgg tctggtgggc gtatgttcca 1740 cctatctggg gcaggtgatc gacgacagct acaacatgaa ccacgtcgat gcgatcaacc 1800 acctgttcgg cattcgtggc tggaccgaac cggtgtcgct gtatcgccag cacgccaacc 1860 gcctgaagaa caagggcgtc tgatcccggt gaacgcggcg gggcaactcg ccgcagtgag 1920 gtcgcatgtc caaattcttc agtctttccc tggttgccgt ggtggtcgct ggcggcctta 1980 ccctgtactg gcgctggccg gcagcagtgc ctgaagcgca gcctgctacc actgtcgccg 2040 tgccgtcacg gcagtcgttg atcgagcagg cttcgacttc ccccgccaag gcgcccgccg 2100 aggcgcaacc gtcaatagcc gtgaccttgc ccagcctggc cggtaccgag gtggatggcc 2160 agctgcgtac cgacgcggcc ggcaatctgc tgctggatct ggcggttcgc gattacttcg 2220 attacttcct gagcgcggtc gatcattccg ggctcgatgc ggtgatcgag gccctgctgg 2280 ccgatgccgg ccggcgcctg ccggagcccg cactcgggca gatgatcagc ctgctcggtg 2340 attaccttga ctacaagcgc gccagcatgg cgctgatgca gcaaccactg gatgcaaggc 2400 agcaggtcga gccgcaggcg cagctacagg ctctgcagtc ggctttcgcg cgcctggacg 2460 agctgcgccg cgcacatttc tctgcaactg cgcaggaagc gctgttcggc gccgaacagg 2520 cctatgcccg ttacaccctc gatagcctgg cagtgcagca gcgtgatgac ctcggcgagg 2580 cgcagcgcac gcaactgctc gaacagttgc gtgagcgtct gcccgatgcg ctgcgcgaaa 2640 gtgagcagcg ccagcaactg gcccaggagc aactgcagcg cagcgagcaa ctctggcgtg 2700 atggtgcgga cgagcaacag gtgcgcgagt tcctggcgat gacctatgat ccggacaccg 2760 tgcagcgtct gcttgatgag cagcgtcgcg agcgcgactg gcagcagcac taccaggcct 2820 atcgcaatga gttggccagc ctgcaaggtc gggggctgag cgaggcagat ggcgagcaac 2880 tgcagcgcca gctgcgcgag cggctgttca gcagtgaaga ccgacaccgt gtggaaacct 2940 atgacgccat tgccgcgaag cagccggagc cgctcgaccc ctgacggatg tcgcccatga 3000 gcgagcgcct gctgttggcc cagcagcgcc gaggctatcg ctcgctgcgt ttcgtcgccg 3060 agctggaggc gggttttgcc gggcatcggg tgcagcgcat tcgtcggcgt ctgccctgat 3120 cgccctacgc 3130 // |
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