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EBI DbfetchID U84350; SV 1; linear; genomic DNA; STD; PRO; 5688 BP. XX AC U84350; XX DT 12-MAR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Amycolatopsis orientalis hypothetical hydroxylases d and b genes, partial DE cds, hypothetical hydroxylase a, glycosyltransferases GtfD (gtfD) and GtfE DE (gtfE) genes, complete cds. XX KW . XX OS Amycolatopsis orientalis OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis. XX RN [1] RP 1-5688 RA Solenberg P.J., Matsushima P., Stack D.R., Wilkie S.C., Thompson R.C., RA Baltz R.H.; RT "Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces RT toyocaensis"; RL Unpublished. XX RN [2] RP 1-5688 RA Solenberg P.J.; RT ; RL Submitted (08-JAN-1997) to the EMBL/GenBank/DDBJ databases. RL Lilly Research Labs, Lilly Corporate Center, Indianapolis, IN 46285, USA XX FH Key Location/Qualifiers FH FT source 1..5688 FT /organism="Amycolatopsis orientalis" FT /strain="C329.4" FT /mol_type="genomic DNA" FT /db_xref="taxon:31958" FT CDS <1..935 FT /codon_start=3 FT /transl_table=11 FT /product="hypothetical hydroxylase d" FT /db_xref="GOA:P96562" FT /db_xref="HSSP:1UED" FT /db_xref="InterPro:IPR017973" FT /db_xref="UniProtKB/TrEMBL:P96562" FT /protein_id="AAB49296.1" FT /translation="ISTYDPPEHTRLRKMLTPEFTVRRIRRMEPAIQSLVDDRLDMLEA FT EGSPADLQGLFADPVGAHALCELLGIPRDDQREFVRRIRRNADLSRGLKARAADSAAFN FT RYLDNLIARQRADPDDGLLGTIVREHGGNVTDEELRGLCTALILGGVETVAGMIGFGVL FT ALLQHPGQIPLLFEGPEKADRVVNELVRYLSPVQAPNPRLAIKDVVVDGQLIKAGDYVL FT CSVLMANRDEALTPDPNVFDANRTAVSDVGFGHGIHYCVGAALARSMLRTAYQTLWRRF FT PGLRLAVPVEEVKYRSAFVDCPDQVPVTW" FT CDS 980..2455 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical hydroxylase a" FT /db_xref="GOA:P96563" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/TrEMBL:P96563" FT /protein_id="AAB49297.1" FT /translation="MSVEDFDVVVAGGGPGGSTVATLVAMQGHRVLLLEKEVFPRYQIG FT ESLLPATVHGVCRMLGVADELANSGFPIKRGGTFRWGARPEPWTFHFGISAKMAGSTSH FT AYQVERAKFDDILLKNAKSKGVVVREGCSVNDVVEDGERVTGARYTDADGNAHEVSARF FT VIDASGNKSRLYTKVNGSRNYSEFFRSLALFGYFEGGKRLPEPVSGNILSVAFDSGWFW FT YIPLSDTLTSVGAVVRREDADKIQGDREKALNTLIAECPLISEYLSNATRVTTGRYGEL FT RVRKDYSYQQDSYWRPGMVLVGDAACFVDPVFSSGVHLATYSALLAARSINSVLAGDLD FT EKTALNEFEARYRREYGVFYEFLVSFYQMNVNEESYFWQAKKVTQNQSTDIESFVELIG FT GVSSGETALTAADRIAANSAEFAAAVDKMATGDGDDMVPMFKSTVVKQAMQEAGQVQMK FT ALLGEDAEPELPLFPGGLVTSPDGMKWLPHHPA" FT CDS 2512..3738 FT /codon_start=1 FT /transl_table=11 FT /gene="gtfD" FT /product="glycosyltransferase GtfD" FT /function="involved in biosynthesis of vancomycin" FT /db_xref="GOA:P96564" FT /db_xref="HSSP:1IIR" FT /db_xref="InterPro:IPR004276" FT /db_xref="UniProtKB/TrEMBL:P96564" FT /protein_id="AAB49298.1" FT /translation="MRVLLSVCGTRGDVEIAVSLAVRLKALGVGTRMCAPPAAAERLAE FT VEVPHVPVGLPQHMMLQEGMPPPPPEEERRLAAMTVEMQFDAIPSAAEGCVAVVAVGDL FT AAATGVRSVAEKLGLPFFYSVPSPVYLASPHFPPPYDEPTTPGVTDHRTLWEERAHRFA FT ERYGETLNRRRAAIGLPPVEDVFGYGHGDRPILSADPVLAPLQPDVDAVQTGAWILTDD FT RPLPPELEAFLAAGPPPVHVGFGSSSGKGIADAAKIAVEVSRAHGRRVILSRGWTDLLL FT PDDREDCFAIGEVNFQALFPRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAAR FT VADLGIGVAHDGPTPTFESLSAALTTVLAPETRARARAVAAMAQTDGAAAAADLVLAAV FT GGNEPAVPA" FT CDS 3791..5017 FT /codon_start=1 FT /transl_table=11 FT /gene="gtfE" FT /product="glycosyltransferase GtfE" FT /function="involved in biosynthesis of vancomycin" FT /db_xref="GOA:P96565" FT /db_xref="HSSP:1IIR" FT /db_xref="InterPro:IPR002213" FT /db_xref="UniProtKB/TrEMBL:P96565" FT /protein_id="AAB49299.1" FT /translation="MRVLLSTCGSRGDVEPLVALAVRLRERGAEVRMCAPPDCADRLAE FT VDVPHLPLGASARPSAGQAKPLTAEDMLRFTTETIATQFERIPAAAEGCAAVVTTGLLA FT AAIGVRSVAEKLGIPYFYGFHCPSYVPSPYYAPPPPLGEPPAPDGTDIQALWERNNQSA FT YRRYGEPLNSRRAAIGLPPVEDIFGHGYTDHPWMAADPVLAPLQPTDLDAVQTGAWILP FT DERPISAELEAFLDAGAPPVYLGFGSLRAPADAAKVAIEAIRAHGHRVILSRGWADLVL FT PDDREDCFAIGEVNQQVLFRRVAAVIHHGGAGTTHVATRAGVPQILVPQIADQPYYAAR FT VAELGVGVAHDGPTPTFDTLSAALTKALAPETRVRAEAVAETVQTDGAAVAADLLFAAV FT TGNQPAVPA" FT CDS 5043..>5688 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical hydroxylase b" FT /db_xref="InterPro:IPR013630" FT /db_xref="UniProtKB/TrEMBL:P96566" FT /protein_id="AAB49300.1" FT /translation="MSTTSRCRICDGTVTEFIDLGRQPLSDAFVVPGDEKKEFFFRLAT FT GICESCTMVQLMEEVPRDLMFHEAYPYHSSGSSVMRTHFQELAKRLLTTELTGEDPFIV FT ELGCNDGIMLKAVAEAGVRQLGVEPSGGVADLAAAKGIRVRKDFFEEATAADIRENDGP FT ADVIYAANTLCHIPYMDSILKGVTTLLRPNGVFVFEDPYLGDIVERTSFDQI" XX SQ Sequence 5688 BP; 889 A; 1942 C; 1996 G; 861 T; 0 other; agatctcgac ctacgatccg cccgagcaca cccggttgcg gaagatgctg acaccggagt 60 tcaccgtccg gcggatccgc cggatggagc ccgcgatcca gagcctcgtc gacgatcgcc 120 tcgacatgct ggaggccgag gggtcgcccg cggacctcca gggcctgttc gccgatccgg 180 tcggcgccca cgccctgtgc gaactgctcg gcatcccccg cgacgaccag cgcgagttcg 240 tccggcggat caggcggaac gccgatctga gccgcggcct caaggcgcgg gccgccgaca 300 gcgcggcgtt caaccggtac ctggacaacc tcatcgcccg gcagcgcgcg gatcccgacg 360 acggactcct cggcacgatc gtgcgagagc acggcggcaa cgtcacggac gaggagttgc 420 ggggcctgtg cacggcgctg atcctcggcg gtgtcgaaac cgtcgcgggg atgatcggct 480 tcggcgtgct cgccctcctt cagcaccccg gccagatacc gttgcttttc gaaggcccgg 540 aaaaggccga ccgcgtcgtc aacgagctgg tgcgctatct gtcccccgtg caggcgccga 600 atccccggct cgccatcaag gacgtggtcg tcgacgggca gctgatcaaa gcgggggact 660 atgtcctgtg ctcggttctc atggcgaaca gggacgaagc actgaccccc gatccgaacg 720 ttttcgacgc gaaccgcacc gctgtttcgg acgtcgggtt cgggcacgga atccattact 780 gcgtcggtgc cgctttggcc cggtcgatgc tgcggacggc ctatcagacc ttgtggcggc 840 gattcccggg gctgcggctg gccgtgcccg tcgaagaagt gaaataccgc agcgcgttcg 900 ttgactgccc cgatcaagta ccggtcacct ggtgacgacc ggcccggcga agtgacactc 960 gacaaaggaa tggtgggaaa tgtcggtcga agatttcgat gttgtggtgg cgggcggcgg 1020 gccgggtggt tcgacggtgg ccaccctggt ggcgatgcag ggacaccggg tcctgctgct 1080 ggagaaagag gtcttccccc ggtaccagat cggtgagtcg ctgctgcccg ccacggtgca 1140 cggggtctgc cggatgctcg gcgtcgcgga cgagctggcg aattccgggt tcccgatcaa 1200 acgcggcggc acgttccgct ggggcgcccg tccggagccg tggacgttcc acttcgggat 1260 ctcggccaag atggcgggct cgacgtcgca cgcctatcag gtcgagcggg cgaagttcga 1320 cgacatcctg ctgaagaacg ccaagagcaa gggcgtcgtc gtgcgggaag gctgctcggt 1380 caacgacgtc gtggaggacg gcgagcgggt caccggcgcg cgctacaccg acgcggacgg 1440 caacgcgcac gaagtctcgg cccggttcgt gatcgacgcg tcgggcaaca agagccggct 1500 ctacacgaag gtcaacggtt cgcggaacta ctcggagttc ttccgcagcc tcgcgctgtt 1560 cggctatttc gagggtggca aacggctgcc cgagccggtg tcgggcaaca tcctgagcgt 1620 cgccttcgac agcggctggt tctggtacat ccccctgagc gacacgctga ccagcgtcgg 1680 cgcggtcgtg cgccgggagg acgccgacaa gatccagggc gaccgcgaga aggccctcaa 1740 caccttgatc gccgaatgcc cgctgatctc ggagtacctc tcgaacgcga ccagggtgac 1800 caccggcagg tacggcgaac tgcgggtgcg caaggactac tcgtaccagc aggacagcta 1860 ctggcggccc gggatggtcc tggtcggcga cgccgcgtgc ttcgtggacc cggtgttctc 1920 ctccggggtg cacctggcga cctacagcgc gctgctcgcg gcccggtcga tcaacagcgt 1980 cctcgcgggc gacctcgacg agaagaccgc gctgaacgag ttcgaggcgc gctatcgccg 2040 cgagtacggc gtcttctacg agttcctcgt ctccttctat cagatgaacg tcaacgagga 2100 atcgtatttc tggcaggcca agaaggtcac gcagaaccag agcaccgaca tcgagtcgtt 2160 cgtcgagctg atcggcgggg tgtcgtccgg cgagaccgcg ctgacggccg ccgaccggat 2220 cgccgcgaac agtgccgaat tcgccgccgc cgtcgacaag atggcgacgg gcgacggcga 2280 cgacatggtg ccgatgttca agtcgaccgt ggtcaagcag gcgatgcagg aggcgggcca 2340 ggtccagatg aaggcgctgc tcggcgagga cgccgaaccc gagctgccgc tgttccccgg 2400 cggcctggtg acttcgcccg acgggatgaa gtggctgccg caccacccgg cctgaaaccc 2460 tccgcacgcc ggcggtgcgc cggtggtcga gcacgaaaca ggggtagcga aatgcgtgtg 2520 ttgttgtcgg tgtgcggaac ccgcggggac gtcgagatcg cggtgtcgct ggcggtccgg 2580 ctgaaggcgc tcggcgtcgg gacgcggatg tgcgcaccgc ccgccgccgc cgagcggctg 2640 gccgaggtcg aggtgccgca tgtgccggtc ggccttccgc agcacatgat gttgcaggag 2700 gggatgccgc cgccgccccc ggaggaggag cggcggctcg cggccatgac ggtcgagatg 2760 cagttcgacg cgatcccctc ggccgccgaa ggatgcgtgg cggtggtggc ggtcggcgat 2820 ctggccgccg cgaccggcgt gcggtcggtg gccgaaaagc tgggcctccc cttcttctac 2880 tccgtcccga gcccggtcta cctggcttcc ccgcacttcc cgccgcccta tgacgagccg 2940 accaccccgg gcgtgaccga ccaccggaca ctgtgggaag agcgtgccca ccggttcgcg 3000 gaacggtacg gggagacgct caaccggcgg cgggccgcga tcggcctgcc gccggtggag 3060 gacgtcttcg gctacggcca cggcgaccgg cccatcctgt cggcggaccc ggtcctcgcc 3120 ccgctgcagc cggacgtcga cgccgtgcag accggcgcgt ggatcctgac cgacgaccgg 3180 ccgcttcccc cggagctgga ggcgttcctg gccgccggcc caccgccggt gcacgtgggt 3240 ttcggcagct cgtccgggaa ggggatcgcc gacgccgcga agatcgccgt cgaggtgagc 3300 cgtgcccacg gccgccgggt gatcctctcc cgagggtgga ccgatctgct cctgcccgac 3360 gaccgggagg actgtttcgc catcggcgag gtgaacttcc aggcgctgtt cccccgggtg 3420 gccgccgtca tccatcacgg cagcgcgggc acggaacacg tggccacgcg ggcgggcgtc 3480 ccccagctcg tgatcccccg gaacaccgac cagccgtact tcgccgccag ggttgccgat 3540 ctggggatcg gtgtggcgca cgacggcccg acgccgacct tcgagtccct gtcggccgcg 3600 ctcaccacgg tcctggcgcc ggaaacgcgc gcgcgggcgc gggccgtggc ggccatggcc 3660 cagaccgacg gcgcggcggc ggccgcggat ctggtgctcg ccgccgtcgg cgggaacgag 3720 cccgccgttc ccgcgtgaac catcagcgac cggacgccgg ccaggaccgg agaaaacggg 3780 gaaatacgtg atgcgtgtgt tgttgtcgac ctgtgggagc cgcggagacg tcgaaccact 3840 ggtggcgttg gcggtgcggt tgcgggagcg cggcgccgag gtgcggatgt gcgcgccgcc 3900 ggactgcgcg gatcggctgg ccgaagtcga cgtgccgcat ctgcccctcg gtgcgtcggc 3960 gcgcccgtcg gccgggcagg cgaaaccctt gacggccgag gacatgctcc ggttcacgac 4020 cgagacgatc gccacgcagt tcgagcggat tccggcggcc gccgaaggat gcgccgcggt 4080 ggtgacgacc ggcctgctgg ccgccgccat cggcgtgcgg tcggtggccg aaaagctggg 4140 catcccctac ttctatggct tccactgccc gagctatgtg ccgtcgccgt actatgcgcc 4200 tccgccgccc ctcggcgagc cgcccgcacc ggacgggacc gacatccagg cgctgtggga 4260 gcgcaacaac cagagcgcct accggcggta cggggagccg ctcaacagca ggcgcgccgc 4320 catcggcctg ccgccggtgg aggacatctt cggccacggc tacaccgatc acccgtggat 4380 ggcggcggac ccggtactgg ccccgctgca acccacggat ctcgacgccg tgcagaccgg 4440 ggcgtggatc ctgcccgacg aacgaccgat ttccgctgag ctggaggcgt tcctggacgc 4500 cggcgcacca ccggtgtacc tggggttcgg cagccttcgc gcccccgccg acgccgcgaa 4560 ggtggccatc gaggcgatcc gtgcccacgg ccaccgggtg atcctctccc gcggctgggc 4620 cgatctggtc ctgcccgacg accgggagga ctgtttcgcc atcggcgaag tgaatcagca 4680 ggtgctgttc cgccgggtgg ccgccgtcat ccaccacggc ggcgcgggca cgacccacgt 4740 ggccacgcgg gcgggcgtcc cccagatcct ggttccccag atcgcggacc agccctacta 4800 cgccgcccgg gtggccgaac tgggggtcgg tgtggcgcat gacggcccga ccccgacctt 4860 cgacacgttg tcggcggcgc tcaccaaggc cctcgctccg gaaacgcgcg tgcgagcgga 4920 agccgtggcg gaaacggtcc agacggacgg ggccgcggtg gccgcggacc tgttgttcgc 4980 cgcggtgacc gggaaccagc ccgccgttcc cgcctgatca tcaccagtac agaggagacc 5040 gcatgtcgac cacttcccgg tgccgcatct gcgacggcac cgtcaccgag ttcatcgatc 5100 tcggacgcca gccgctttcg gacgcgttcg tggttcccgg cgacgagaag aaggagttct 5160 tcttccggct cgccacgggg atctgcgagt cctgcacgat ggtgcagctg atggaagagg 5220 tcccgaggga cctgatgttc cacgaggcgt acccctatca ctcgtccggt tcgtcggtca 5280 tgcgcacgca tttccaggaa ctggccaagc gcctgctgac cacggagctg accggcgagg 5340 acccgttcat cgtcgagctg gggtgcaacg acggcatcat gctcaaggcc gtggccgaag 5400 ccggggtacg ccagctcggt gtggaaccct ccggcggcgt cgcggatctg gccgcggcca 5460 agggaatccg cgtccgcaag gatttcttcg aagaggcgac ggcggccgac atccgggaga 5520 acgacggccc cgccgacgtg atctacgcgg ccaacaccct gtgccacatc ccttacatgg 5580 actcgatcct caagggcgtc acgacgctgc tgcgcccgaa cggcgtgttc gtgttcgagg 5640 acccgtacct cggcgacatc gtggagcgca cgtcgttcga ccagatct 5688 // |
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