 |
|
EBI DbfetchID U82366; SV 1; linear; genomic DNA; STD; PRO; 4093 BP. XX AC U82366; XX DT 04-JUN-1997 (Rel. 52, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Lactobacillus sake ptsHI operon: histidyl phosphocarrier protein HPr (ptsH) DE and enzyme I (ptsI) genes, complete cds. XX KW . XX OS Lactobacillus sakei OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-4093 RX PUBMED; 9172326. RA Stentz R., Lauret R., Ehrlich S.D., Morel-Deville F., Zagorec M.; RT "Molecular cloning and analysis of the ptsHI operon in Lactobacillus sake"; RL Appl. Environ. Microbiol. 63(6):2111-2116(1997). XX RN [2] RP 1-4093 RA Stentz R.; RT ; RL Submitted (16-DEC-1996) to the EMBL/GenBank/DDBJ databases. RL Stentz R., Institut National De La Recherche Agronomique, Laboratoire De RL Recherche Sur La Viande, Domaine De Vilvert, Jouy En Josas, France, 78350 XX FH Key Location/Qualifiers FH FT source 1..4093 FT /organism="Lactobacillus sakei" FT /strain="160*1" FT /mol_type="genomic DNA" FT /db_xref="taxon:1599" FT CDS complement(<1..600) FT /codon_start=1 FT /transl_table=11 FT /note="similar to the proteins belonging to the Clp family; FT ORFX" FT /db_xref="HSSP:1JBK" FT /db_xref="UniProtKB/TrEMBL:O07124" FT /protein_id="AAC45389.1" FT /translation="MLCQNCQKNPATIHLYTSVNGQNQAINLCQNCYQALKQQGMSSQY FT NDPFGFSNLDELFNAINNPNAERNAANQGPQTQGGRNGGGNNGQNGESLFSQFGVNLTN FT LAKGGQIDPVIGRDKEIARVIEILNRRTKNNPVLIGEAGVGKTAVVEGLAQKIADGDVP FT AKLKDRQVIRLDVVSLVQGTGIRGQFEERMQQLITEL" FT RBS complement(615..620) FT /note="putative; ORFX" FT -10_signal complement(674..679) FT /note="putative; ORFX" FT -35_signal complement(696..701) FT /note="putative; ORFX" FT -35_signal 1054..1059 FT /gene="ptsH" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 1077..1082 FT /gene="ptsH" FT /experiment="experimental evidence, no additional details FT recorded" FT mRNA 1091..3213 FT /note="ptsHI operon" FT /experiment="experimental evidence, no additional details FT recorded" FT mRNA 1091..1781 FT /gene="ptsH" FT /note="transcription start site determined by primer FT extension analysis" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 1153..1158 FT /gene="ptsH" FT /note="putative" FT CDS 1168..1434 FT /codon_start=1 FT /transl_table=11 FT /gene="ptsH" FT /product="HPr" FT /function="histidyl phosphocarrier protein" FT /note="histidine-containing or heat stable protein." FT /db_xref="GOA:O07125" FT /db_xref="HSSP:1QFR" FT /db_xref="InterPro:IPR002114" FT /db_xref="UniProtKB/Swiss-Prot:O07125" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC45390.1" FT /translation="MEKRDFHVVADTGIHARPATLLVQTASKFNSDVNLEYKGKSVNLK FT SIMGVMSLGVGQGADVTISAEGADEADAINAIEETMKKEGLSE" FT RBS 1415..1420 FT /gene="ptsH" FT /note="putative" FT CDS 1434..3158 FT /codon_start=1 FT /transl_table=11 FT /gene="ptsI" FT /product="enzyme I" FT /function="histidyl phosphocarrier protein" FT /db_xref="GOA:O07126" FT /db_xref="HSSP:1EZC" FT /db_xref="InterPro:IPR015813" FT /db_xref="UniProtKB/Swiss-Prot:O07126" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC45391.1" FT /translation="MTKLRGIAASDGIATAKAYMLVQPDLSFSKSTISDSEKEINRLHK FT ALQDSTSDLETIRKIAAESLGEEEAQVFDAHMMILADPEFTGAIEGKINDDKVNAEQAL FT KEVADLFVATFESMTNNAYMQERAADIKDVTKRVLSHLLGVTLPNPALIDEEVIVIAHD FT LTPSDTAQLNGKFVKAFVTDVGGRTSHSAIMARSLEIPAIVGTETVTQDVKAGDLLIVD FT GINGDVVLDPTDADIAEYDVKAQAFADQKAEWEKLKNEKSVTKDGKTFTVAANIGTPKD FT LAGVLENGSEAIGLYRTEFLYMDSAELPSEDDQFEAYKSVLEGMDGKPVVVRTMDIGGD FT KKLPYLPLPEEMNPFLGYRAIRISLDRDDIFRTQLRALLRASNYGKLRIMFPMIATVAE FT FRQAKGILEDEKAKLIAAGQTVSDDLQVGMMVEIPASAVLANQFAKEVDFFSIGTNDLI FT QYTMAADRMNERVSYLYQPYNPAILRLIKNVIDASHKEGKWTGMCGEAAGDSIMAPLLV FT GMGLDEFSMSATSVLRVRSLMKRLDTTELTDLVETAVNVNTSNEENQKLVEDFMKDR" FT terminator 1743..1781 FT /gene="ptsH" FT /experiment="experimental evidence, no additional details FT recorded" FT terminator 3172..3213 FT /note="ptsHI operon" FT /experiment="experimental evidence, no additional details FT recorded" FT -35_signal 3248..3253 FT /note="putative; ORFY" FT -10_signal 3273..3278 FT /note="putative; ORFY" FT RBS 3292..3297 FT /note="putative; ORFY" FT CDS 3309..3749 FT /codon_start=1 FT /transl_table=11 FT /note="similar to CopY, the negative regulator of copYZAB FT operon of Enterococcus hirae; ORFY" FT /db_xref="GOA:O07127" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:O07127" FT /protein_id="AAC45392.1" FT /translation="MAENTNEITTSEWEVMRIVWSLGQVNSRDLIDLLQPKRDWQDSTI FT KTLIGRLVKKGFLKTEKEGRRFNYTATVPEIEAMENATQSLFEHLCGMKKGQTLAALID FT QTTLSQTDILQLQQLLTAKAATAPEKVACDCLPNKCDCEKEE" FT RBS 3740..3745 FT /note="putative; ORFZ" FT CDS 3752..>4093 FT /codon_start=1 FT /transl_table=11 FT /note="similar to P-type ATPases proteins including CopB of FT Enterococcus hirae; ORFZ" FT /db_xref="GOA:O07128" FT /db_xref="InterPro:IPR001757" FT /db_xref="UniProtKB/TrEMBL:O07128" FT /protein_id="AAC45393.1" FT /translation="MKHDDMKMHDMHDMDHDHGDMMMHGGHMMHMGNLKQKFWVSLVAT FT IPIIILSPFMGIKLPFQTTFPGSDWLVLLLATFLFFYGGAPFLKRCKERIGREEPSDDD FT VDCMGITVSY" XX SQ Sequence 4093 BP; 1281 A; 742 C; 899 G; 1171 T; 0 other; gagctcagtg atcaactgtt gcatccgttc ttcaaattga ccgcgaatac cagtcccttg 60 tactaatgaa acgacatcca agcgaatcac ttggcgatcc tttaatttag caggaacgtc 120 gccatcggca attttttgcg ctaaaccttc gacaacggct gttttaccaa cgccggcttc 180 accaattaag actggattat tcttggtacg ccgattgaga atttcgatga cgcgcgcgat 240 ttccttatcg cgaccaatca ccggatcaat ttgaccaccc ttagcaaggt tggtgaggtt 300 cacaccaaat tggctgaata agctttcgcc attttgaccg ttgttaccgc cgccattacg 360 gccaccttgt gtttggggac cttggttagc agcattacgt tcagcgtttg gattgttgat 420 tgcattgaag agttcatcaa gattactaaa accaaaggga tcattatatt gactactcat 480 tccttgttgc tttagtgctt gataacagtt ttgacaaagg ttaatggctt ggttttgacc 540 attgacgcta gtgtaaagat ggattgttgc tggatttttt tggcaatttt gacaaagcat 600 ttaatctgtc acaacctttc gcctattaaa tgtaacgtca aagtttgttt gactatcttt 660 gaccttgaat aaattataat catctctaaa aaaagttgca agtaatcgaa ctcgaaaata 720 aaactttatt tgtgggtcaa aattgcgtac aattggaggc agtgacgcta cttgataaat 780 gaaaagaggg tcattaaaaa taattatcta gacgtgataa aggagtgatt cagatggcaa 840 gagatttttt agcggagatg gatgccttgt taaatcacga gaaagaaacc attgtcgttg 900 aacatgatga gtttttagac tttcagaaag tatgggtcaa ttatgatcac cgaaaagaaa 960 ttgtaggcgc tgcccaacga aatggacaag tgatttatcg ctgcgctgaa agcggtatag 1020 tgtagactat cattaatttt gttagcgaag ctgttgtagt acttgtctcc acatggtaat 1080 cttaatattc gaaagggtag acactattga tgcctactta tttcggccca cacaaatcta 1140 taaatagata aaaggggatc gattaacatg gaaaaacgcg attttcacgt agtagcagac 1200 acaggtatcc acgctcgtcc agctacatta ttggtacaaa cagcaagcaa atttaactca 1260 gatgtcaact tagaatacaa aggtaaatct gtaaacttga aatcaatcat gggcgttatg 1320 tcacttggtg ttggccaagg cgcagacgtt acaatttctg ctgaaggtgc tgatgaagca 1380 gacgcaatca acgcaatcga agaaacaatg aaaaaggaag gtttatctga ataatgacta 1440 aactaagagg gattgctgcc agtgatggta tcgctactgc gaaagcctac atgttagtgc 1500 aacccgattt gtcattttcg aaatcaacta tttcagattc agaaaaagag attaatcgtt 1560 tgcataaggc cttacaagat tctacgagtg atctagagac cattcggaag attgctgctg 1620 aatcattagg agaagaagaa gcacaagttt ttgacgctca catgatgatt ttagcagacc 1680 cagaatttac cggcgctatc gaaggtaaaa tcaatgatga caaggttaat gctgagcaag 1740 cgttgaaaga ggtcgcggat ttattcgtgg ccacttttga gtctatgact aataacgcat 1800 acatgcaaga acgtgctgct gacatcaaag atgttacgaa gcgagttctc agtcatttat 1860 taggggtaac actccctaac ccagcattaa ttgatgaaga agtaattgtg attgctcatg 1920 acttgacacc tagtgacaca gcccaattaa acggtaaatt cgttaaggct tttgtaactg 1980 atgttggtgg tcggacgtca cattctgcca ttatggcacg ttctttagaa atccctgcaa 2040 ttgttgggac tgaaacagtc acacaagatg ttaaagctgg cgacttatta attgtcgatg 2100 gtattaacgg tgatgttgtt ttagatccta cagatgctga tattgctgaa tatgatgtga 2160 aggcccaagc ctttgctgat caaaaagcag aatgggaaaa actaaagaat gaaaagtctg 2220 tcactaaaga cggcaaaaca tttacggttg ctgctaatat cggaacacct aaagatttag 2280 ctggtgttct tgaaaatggc tctgaagcaa tcggtttata tcggacagaa ttcttatata 2340 tggattcagc tgagttacct agtgaagatg accagttcga agcttataaa tcagttctag 2400 aaggtatgga cggcaaacca gttgttgtgc ggacaatgga tattggtgga gataagaaat 2460 taccttactt accattacca gaagaaatga atccgttctt aggctaccga gcaattcgga 2520 ttagtttaga tcgtgatgat attttcagaa cacaattgcg cgccttatta cgtgcttcta 2580 actatggtaa gttacggatc atgttcccaa tgatcgcaac tgttgctgaa ttccgtcaag 2640 ctaaagggat tctcgaagat gaaaaagcaa aattaattgc tgcaggtcaa actgtatctg 2700 acgatttaca agttgggatg atggttgaaa ttccagccag cgccgtattg gctaaccaat 2760 ttgctaaaga agtcgatttc ttcagtatcg ggacgaacga cttgattcaa tacacaatgg 2820 ctgctgatcg gatgaacgaa cgtgtttcat acctttacca accttataat ccagcaatct 2880 tacgtcttat taagaacgtc attgacgctt ctcataaaga aggtaagtgg actggtatgt 2940 gtggggaagc tgccggagat tcaatcatgg caccactcct tgtcgggatg ggcttagatg 3000 aattctcaat gagtgcaact tctgtgttac gagttcgtag tttgatgaaa cgtttagata 3060 caactgaatt aactgattta gttgaaacag ctgttaacgt caatacaagc aacgaagaaa 3120 atcaaaaatt agtcgaagat tttatgaaag atcgttaggc ttaaactttt taatgggtgt 3180 taagacaaaa gtaattttgt cttaacaccc tttttttgta ccccaaaagt attaatgatt 3240 gacagaagtg cttcttgatt gtaaactgaa tatataatta caaatgtaaa cgaaagagtg 3300 tgagcaagat ggcagaaaat acaaatgaaa ttacgacttc cgaatgggaa gtgatgcgca 3360 ttgtctggtc acttggtcaa gtcaacagtc gtgacttgat tgatttactt caaccaaagc 3420 gcgattggca agattcaacc atcaaaacat tgattggccg cttggttaaa aaaggcttct 3480 taaaaacaga aaaggaaggg cggcggttta attacacagc aaccgttcct gagattgaag 3540 cgatggaaaa tgccacacaa agtctttttg agcacttgtg tggcatgaaa aaagggcaga 3600 cattagcggc tttaattgat caaacgactt tgagtcagac ggatattttg cagttacaac 3660 aattactgac agccaaagca gctactgcac cagaaaaagt ggcttgcgac tgtttgccaa 3720 ataaatgtga ctgtgaaaag gaggaataac gatgaaacac gatgatatga agatgcacga 3780 tatgcatgat atggaccatg atcacggcga tatgatgatg catggtggac atatgatgca 3840 catgggtaat ttgaaacaga aattctgggt ctcattagtc gcaacaattc ccattattat 3900 attatcgcct tttatgggaa ttaaattacc gtttcaaacc acgttcccag gatcggattg 3960 gttagtcctc ttattagcaa ccttcctatt cttttacggc ggcgcgcctt ttcttaaaag 4020 gtgcaaagag cgaattggcc gagaagaacc cagcgatgat gacgttgatt gcatggggat 4080 tacggtatcg tac 4093 // |
|