 |
|
EBI DbfetchID U82255; SV 1; linear; genomic DNA; STD; PRO; 4468 BP. XX AC U82255; XX DT 06-JAN-1999 (Rel. 58, Created) DT 17-AUG-2002 (Rel. 72, Last updated, Version 4) XX DE Anaerobic thermophile KM-THCJ s-layer associated multidomain endoglucanase DE (celA) gene, complete cds. XX KW . XX OS Thermoanaerobacterium polysaccharolyticum OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; OC Thermoanaerobacterales Family III. Incertae Sedis; Thermoanaerobacterium. XX RN [1] RP 1-4468 RX PUBMED; 10049399. RA Cann I.K., Kocherginskaya S., King M.R., White B.A., Mackie R.I.; RT "Molecular cloning, sequencing, and expression of a novel multidomain RT mannanase gene from Thermoanaerobacterium polysaccharolyticum"; RL J. Bacteriol. 181(5):1643-1651(1999). XX RN [2] RP 1-4468 RA Cann I.K.O., White B.A., Mackie R.I.; RT ; RL Submitted (13-DEC-1996) to the EMBL/GenBank/DDBJ databases. RL Department of Animal Sciences, University of Illinois at Urbana-Champaign, RL 1207 West Gregory Drive, Urbana, IL 61801, USA XX FH Key Location/Qualifiers FH FT source 1..4468 FT /organism="Thermoanaerobacterium polysaccharolyticum" FT /strain="KM-THCJ" FT /mol_type="genomic DNA" FT /db_xref="taxon:44256" FT CDS 586..3879 FT /codon_start=1 FT /transl_table=11 FT /gene="celA" FT /product="s-layer associated multidomain endoglucanase" FT /function="hydrolysis of substrates such as carboxymethyl FT cellulose, xylan, lichenan and laminarin" FT /db_xref="GOA:Q9ZA17" FT /db_xref="InterPro:IPR001119" FT /db_xref="InterPro:IPR001547" FT /db_xref="InterPro:IPR003305" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="PDB:2ZEW" FT /db_xref="PDB:2ZEX" FT /db_xref="PDB:2ZEY" FT /db_xref="PDB:2ZEZ" FT /db_xref="UniProtKB/TrEMBL:Q9ZA17" FT /protein_id="AAD09354.1" FT /translation="MKKVLSKLMVFVMVLTVALGNGVFIGDKQAKAAGTSGDGRFHVVG FT NKIVDPDGNDFVIKGVNIQGYRSWEKRSVLQDVHLIADVWKFNTVRLNCFIGQNNWGEG FT TGANNDIDAIIKAFTAKKVVVEIDLHDTTGYPPLSNPPPAPGQPSLDQAIAWFKELAAK FT YKDNPYVWFNTMNEPGSSTAPLDPQWKVANEEIIKAIRSTGADNIIVVDGWSYANEGIE FT QNTPTVDEKRSAVLTYGQDLLNADSAKNTIFAFHNYNEGDIQKKVEDYIDRANAKGLYV FT FMEEYGKDYSDAAKEGVKSGLQAVMNKGAGRIYWNWDGYDLYDLTSGTGRGSGWEINKT FT DGSKPTNLSWVGDKIWDDNHGITPTFDDQNPKVDLALERLIANNNGFKAGDKVQFTTFL FT RNSGDLPIGKDSKVVVKFYVDGVQLGDPVEISGGIAVGQRIPVTSPELTVSKTDFTVKA FT VIDSSSTYADGAEDAVIENNWIEAAFNSTAPSSGYELVVTGIKITPDTVKERDYVQAQV FT TVANQGPEATPVTNIIGWFYVNGYYYTLDDAAKSCAEQDNVTLKPGESITLSTKVKYRA FT AAPCNFSFVLENLYADDQNQSNNSITVNVPVKMGEGGVNMVSNPGFEDGLDSWQDWQQD FT MSAVPEAAHNGALGLKIGGGKAAGGGQDIPLKPNTTYILGAWAKFDSKPAGTFDVVVQY FT HLKDANNTYVQHILNFNETDWTYKQLLFTTPDVFGSTPQLALWKGDTSKANLYVDDVYL FT VEVSNLIVNGTAENGMDGWPDWGYPVSAVPEAAYGGTKGFKLSGGKQAGMGQKVALKPN FT TTYILGAWGKFTAKPGTYCDVIVQYHLKDANNTYVQNILRFTETDWTYKQVVFTTPDAF FT GSDPEFVLWKDDASNADFYADNITLVEVPSSMVNKGSIAPKFTDISSSWAKNEIQVLAS FT KNIISGYPDGTFKPDKRITRAEFVSMLVKALGIKQSIPDVPTFSDVNKGDWYYGLVEAA FT KSTGIASGYGKQFKPDMQITRQEMMVMVVNALRVNKVEKFVSKGDVSVLGKFKDGGKVQ FT NWAKDAMAIGVSNGLIKGTGDEYLSPDGRATRAQAAAVIYRMLLQLGRI" FT misc_feature 2494..3282 FT /gene="celA" FT /note="Region: polysaccharide binding site" XX SQ Sequence 4468 BP; 1316 A; 857 C; 1199 G; 1096 T; 0 other; gaattctacc agatggaaaa cgcctttttg ccctttaaca ggaatggtgt gctgtttccg 60 cgtaagataa agggtaagta cgcgatgttg agccggccta gcgacaacgg gcatacgcct 120 tttggcgata tattctacag cgagagcccg gatatggtgc actgggggca ccacaggcac 180 gtgatgtcgc ccaacaagcc gtgggagagc accaagatag gagcaggtcc tgtgcctata 240 gagacgacgg aaggctggtt gctgttctac catggggttt tgacatcgtg caacgggttt 300 gtgtacagct ttggagccgc tttactggac ttagatgagc cgtggaaggt gatatacagg 360 gcatcggatt acctgctgtc accgcaaaag ctgtacgagt gcgtagggga tgtgcccaat 420 gtggtatttc ccacagcggc gttgtacgat gcgcccacgg gaaggatagc gatctactac 480 ggagctgcgg atacggttac atgtcttgcc ttcgccaaag tagacgaagt gatagatttt 540 gtaaaatcca ataactctct gtgatttaag gcggtgtttg aaaaaatgaa aaaagttttg 600 tccaagttga tggtttttgt aatggtattg actgttgctt taggcaacgg tgtatttatc 660 ggtgacaagc aggcaaaagc tgccgggact tccggtgatg ggcgctttca cgtagtgggc 720 aataaaatcg ttgaccccga tggaaatgat tttgtaatca aaggcgtgaa catccaggga 780 taccgctcct gggagaagag aagcgtcctt caggatgttc atctgatcgc tgatgtatgg 840 aaatttaata cagtgaggct taactgcttt atcgggcaaa acaactgggg ggaggggact 900 ggcgccaata acgatattga cgccatcatt aaggccttta cggctaaaaa agtggtagta 960 gaaattgacc ttcacgacac cacggggtat ccgccattga gcaatcctcc tccagcgcca 1020 ggacagccca gtttagatca ggctattgcg tggtttaagg aattggccgc taaatacaaa 1080 gacaaccctt atgtctggtt taacaccatg aatgagcctg gctcgtctac cgctccgcta 1140 gacccacagt ggaaagtggc taatgaagag atcattaaag ccataaggtc tacaggggca 1200 gacaatatta tagtggtgga tggttggagc tatgccaacg aaggcattga gcaaaacaca 1260 cctacggtag atgaaaagag aagcgcggtt ttgacctacg gtcaggatct gttaaacgcg 1320 gacagtgcta aaaacacgat ttttgccttt cacaactata acgaaggcga tatccagaaa 1380 aaagttgaag attacataga cagagctaat gcgaagggct tgtacgtgtt catggaggaa 1440 tacggcaagg attacagcga tgccgcgaaa gaaggcgtaa agtctggctt acaggcagtg 1500 atgaacaaag gtgccggaag gatatattgg aactgggatg gttacgattt atacgatctg 1560 acttcaggta ccggaagggg aagcggttgg gagatcaata aaaccgatgg gtccaagccc 1620 actaatctga gctgggtagg ggataagata tgggatgaca atcatgggat tacgccgacc 1680 tttgatgatc aaaatcccaa ggtggatctt gctctggaga gattgatcgc caataacaac 1740 gggtttaaag caggcgataa agtccagttt accacgtttt tacgcaattc aggggatttg 1800 cccattggaa aagatagcaa agtcgttgtc aaattttacg tagatggcgt gcagctggga 1860 gatcctgtgg aaatcagcgg gggaatagct gtaggccaga ggatacctgt aacgtctccc 1920 gaactcactg tttctaagac cgattttacg gtaaaagctg ttatagatag ttcaagtacc 1980 tatgctgatg gtgcagagga tgcggttatt gaaaacaatt ggattgaagc cgcgtttaat 2040 agcactgctc cttcttctgg ctatgaactg gttgtaaccg ggataaaaat aactcctgat 2100 acggtaaagg aaagagatta tgtgcaggct caggtcacgg ttgccaatca gggccctgag 2160 gccacacctg tcacaaatat aataggatgg ttctacgtca acggctacta ttatacgtta 2220 gatgacgccg ctaaatcctg tgcagaacag gacaatgtga ccttaaaacc gggtgagtca 2280 ataactttaa gcacaaaggt taaatatagg gcggcggctc cttgtaactt tagttttgtg 2340 ctggaaaatt tatacgctga tgatcagaac caatctaata actccattac agtaaacgtg 2400 ccggttaaga tgggcgaagg cggcgtaaac atggtgagca acccgggctt tgaagacggt 2460 ctggacagct ggcaagactg gcagcaggac atgagcgcag tgccagaggc ggctcataat 2520 ggcgctttgg ggcttaaaat aggtggtgga aaggcagcag gcggtgggca ggatataccg 2580 ttaaagccca ataccactta tatcttaggg gcatgggcta aatttgacag caagcctgca 2640 gggacgtttg atgtggtggt acagtatcac ttaaaggatg caaacaatac ctatgtccag 2700 cacattttga attttaacga gacggattgg acatataagc agctgctgtt tactacaccc 2760 gacgtattcg gatcaacgcc gcagctggcc ctttggaaag gggatacgtc aaaagccaac 2820 ctgtacgtgg atgatgtata tctcgtggaa gtttccaatt taatagtgaa cggaacagca 2880 gagaacggga tggacggatg gcctgattgg ggttatccgg ttagcgctgt acctgaggcc 2940 gcttatgggg gcactaaggg gtttaagctg tcaggcggaa aacaggcagg aatgggccag 3000 aaagtcgctt taaagcccaa taccacttat atcttagggg catggggtaa atttaccgct 3060 aaacctggta cttactgcga tgtcatagtt cagtatcacc tgaaggacgc caataatacc 3120 tatgtgcaaa acatattgag gtttactgaa acggactgga cttataagca ggtcgtattt 3180 accacccctg atgcatttgg atcagatcca gaatttgtgc tgtggaaaga tgatgcgtca 3240 aacgccgatt tttacgccga taatatcacg ctcgttgaag taccttcctc tatggtcaat 3300 aaaggcagta tagcgccgaa atttactgat atatcaagca gttgggcaaa aaacgagata 3360 caggttctcg catctaaaaa cataatatcg ggttatcctg acggtacatt taagcctgac 3420 aagagaataa cgagagctga attcgtatcc atgcttgtaa aggccctggg gataaagcaa 3480 agcatacctg atgtccccac cttcagcgat gtcaacaagg gagattggta ttatggtttg 3540 gtggaagccg ctaagagtac agggatcgca tcaggatatg gaaagcagtt taagcctgat 3600 atgcaaatta caaggcagga aatgatggta atggttgtaa acgccttgag ggttaacaaa 3660 gtagaaaaat ttgtatccaa aggcgatgtg tcggtactgg gcaaattcaa agatggcggc 3720 aaggtgcaaa attgggcaaa agacgcgatg gctataggcg tgagtaatgg cttgattaaa 3780 ggcactggcg atgagtatct gtctcctgac gggagggcta ctagggctca agctgctgca 3840 gtgatataca ggatgttgtt gcagcttggt aggatatagc gttgatagtt aggaggtata 3900 ctatgagtta cgataacttc gagttagcca tatattgcac cgtacactac cttgaaagta 3960 taaacgatat ttctcaactg gcgcaggatt ttaagcaatt tgagaagcac ttaaagtgca 4020 gcaaagtata cctggagacc tttcgcgcgg atttgcaggg agaagatggt taaaataaaa 4080 gaattatttt aagaacaaag gcataaaggt ttcaggcggg attactactg tagcccagaa 4140 aggattatcg ggttttgctg cattttgcta cacaaattca aaaaacaggg agaaggttaa 4200 gggatattgt ccggtttacc gccgagttat ttgatgaaat catattagat gactttttct 4260 ttaccaattg caaatgccaa tcgtgcatac aagctaaagg cgacaaaacg tggagtgagt 4320 tcaggacgca gttattaaaa gaggtatcct gtgagctggt agacgcagcc aaagaggtta 4380 accctgatgt aaggctgatt atcaaatacc ccaattggta tgaccattat cagaatacag 4440 ggtataactt aaaggatcaa ccgcagat 4468 // |
|