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EBI DbfetchID U72152; SV 1; linear; genomic DNA; STD; PRO; 4407 BP. XX AC U72152; M37773; M55988; M63192; XX DT 03-JAN-1997 (Rel. 50, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Vibrio cholerae iron-regulated virulence proteins (irgB) and (irgA) and DE erythrose-4-phosphate dehydrogenase (epd) genes, complete cds and DE phosphoglycerate kinase (pgk) gene, partial cds. XX KW dehydrogenase; iron; transcriptional regulation; virulence. XX OS Vibrio cholerae OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; OC Vibrio. XX RN [1] RP 1-1530 RX PUBMED; 2174861. RA Goldberg M.B., Boyko S.A., Calderwood S.B.; RT "Transcriptional regulation by iron of a Vibrio cholerae virulence gene and RT homology of the gene to the Escherichia coli fur system"; RL J. Bacteriol. 172(12):6863-6870(1990). XX RN [2] RP 1-1100 RX DOI; 10.1073/pnas.88.4.1125 RX PUBMED; 1705025. RA Goldberg M.B., Boyko S.A., Calderwood S.B.; RT "Positive transcriptional regulation of an iron-regulated virulence gene in RT Vibrio cholerae"; RL Proc. Natl. Acad. Sci. U.S.A. 88(4):1125-1129(1991). XX RN [3] RP 905-3077 RX DOI; 10.1111/j.1365-2958.1992.tb01415.x RX PUBMED; 1406279. RA Goldberg M.B., Boyko S.A., Butterton J.R., Stoebner J.A., Payne S.M., RA Calderwood S.B.; RT "Characterization of a Vibrio cholerae virulence factor homologous to the RT family of TonB-dependent proteins"; RL Mol. Microbiol. 6(16):2407-2418(1992). XX RN [4] RP 1-4407 RX PUBMED; 8982014. RA Carroll P.A., Zhao G., Boyko S.A., Winkler M.E., Calderwood S.B.; RT "Identification, sequencing, and enzymatic activity of the RT erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae"; RL J. Bacteriol. 179(1):293-296(1997). XX RN [5] RP 1-4407 RA Carroll P.A., Boyko S.A., Calderwood S.B.; RT ; RL Submitted (20-SEP-1996) to the EMBL/GenBank/DDBJ databases. RL Infectious Disease, Massachusetts General Hospital, Fruit Street, Boston, RL MA 02114, USA XX FH Key Location/Qualifiers FH FT source 1..4407 FT /organism="Vibrio cholerae" FT /strain="O395" FT /mol_type="genomic DNA" FT /db_xref="taxon:666" FT terminator complement(9..38) FT /gene="irgB" FT CDS complement(36..932) FT /codon_start=1 FT /transl_table=11 FT /gene="irgB" FT /product="iron-regulated virulence protein" FT /note="IrgB; member of LysR family; iron-regulated FT transcriptional activator" FT /db_xref="GOA:A5F9F9" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:A5F9F9" FT /protein_id="AAC44765.1" FT /translation="MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLG FT QSLLMRQGNRLTLTKAGEVFAVYSEQLLELANKSQEALQELNNQVTGELTLVVHPNLIR FT GWLSQVLDEFMQQHSTLKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYWRYA FT TYASPKYLAHRDKPTHPRELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDN FT LAMQADAIAKGRGIGLLPTWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLR FT LRLFIDALRQARPDEWQ" FT mRNA complement(<36..1047) FT /gene="irgB" FT /product="iron-regulated virulence protein" FT RBS complement(942..947) FT /gene="irgB" FT -10_signal 1027..1032 FT /gene="irgA" FT /note="no -35 signal was identified for irgA, transcription FT of irgA requires a trans-acting positive activator, irgB FT which is upstream and divergent from irgA" FT mRNA 1040..>3055 FT /gene="irgA" FT /product="iron-regulated virulence protein" FT /note="iron-regulated outer membrane protein" FT misc_signal 1041..1059 FT /phenotype="potential role in iron regulation" FT /function="dyad similar to E.coli Fur binding sites" FT /note="irgB and irgA genes" FT -10_signal complement(1054..1059) FT /gene="irgB" FT RBS 1066..1070 FT /gene="irgA" FT CDS 1077..3035 FT /codon_start=1 FT /transl_table=11 FT /gene="irgA" FT /product="iron-regulated virulence protein" FT /note="IrgA; iron-regulated outer membrane protein" FT /db_xref="GOA:A5F9G0" FT /db_xref="HSSP:1NQE" FT /db_xref="InterPro:IPR010917" FT /db_xref="UniProtKB/Swiss-Prot:A5F9G0" FT /protein_id="AAC44766.1" FT /translation="MSRFNPSPVSLSVTLGLMFSASAFAQDATKTDETMVVTAAGYAQV FT IQNAPASISVISREDLESRYYRDVTDALKSVPGVTVTGGGDTTDISIRGMGSNYTLILV FT DGKRQTSRQTRPNSDGPGIEQGWLPPLQAIERIEVIRGPMSTLYGSDAIGGVINIITRK FT DQQQWSGNVQLSTVVQENRASGDEQSANFFVTGPLSDALSLQVYGQTTQRDEDEIEHGY FT GDKSLRSLTSKLNYQLNPDHQLQLEAGVSAQDRENNVGKSAQSSGCRGTCSNTDNQYRR FT NHVAVSHQGDWQGVGQSDTYLQYEENTNKSREMSIDNTVFKSTLVAPIGEHMLSFGVEG FT KHESLEDKTSNKISSRTHISNTQWAGFIEDEWALAEQFRLTFGGRLDHDKNYGSHFSPR FT VYGVWNLDPLWTVKGGVSTGFRAPQLREVTPDWGQVSGGGNIYGNPDLQPETSINKELS FT LMYSTGSGLAASLTAFHNDFKDKITRVACPANICTAGPNQWGATPTYRVNIDEAETYGA FT EATLSLPITESVELSSSYTYTHSEQKSGNFAGRPLLQLPKHLFNANLSWQTTDRLNSWA FT NLNYRGKEMQPEGGASNDDFIAPSYTFIDTGVTYALTDTATIKAAVYNLFDQEVNYAEY FT GYVEDGRRYWLGLDIAF" FT -35_signal complement(1078..1083) FT /gene="irgB" FT misc_feature 1173..1193 FT /gene="irgA" FT /function="potential interaction site of IrgA with FT TonB-like protein" FT /note="putative TonB box; region of the protein similar to FT the TonB box of TonB-dependent outer membrane proteins of FT E. coli" FT terminator 3048..3077 FT /gene="irgA" FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 3187..4212 FT /codon_start=1 FT /transl_table=11 FT /gene="epd" FT /product="erythrose-4-phosphate dehydrogenase" FT /db_xref="GOA:A5F9G1" FT /db_xref="HSSP:1HDG" FT /db_xref="InterPro:IPR000173" FT /db_xref="UniProtKB/Swiss-Prot:A5F9G1" FT /protein_id="AAC44767.1" FT /translation="MLRVAINGFGRIGRNVLRAVYESGKRDRIQVVAVNELAKPDAMAH FT LLQYDTSHGRFGKKISHDQQHIYVHHQNGEYDSIRILHLSEIPLLPWRDLGVDLVLDCT FT GVYGCQEDGQQHIDAGAKLVLFSHPGASDLDNTIIYGVNHETLTAEHKIVSNGSCTTNC FT IVPIIKVLDDAFGIDSGTITTIHSSMNDQQVIDAYHNDLRRTRAASQSIIPVDTKLHKG FT IERIFPKFSNKFEAISVRVPTVNVTAMDLSVTIKSNVKVNDVNQTIVNASQCTLRGIVD FT YTEAPLVSIDFNHDPHSAIVDGTQTRVSNGQLVKMLVWCDNEWGFANRMLDTALAMQAT FT Q" FT CDS 4361..>4407 FT /codon_start=1 FT /transl_table=11 FT /gene="pgk" FT /product="phosphoglycerate kinase" FT /db_xref="GOA:A5F9G2" FT /db_xref="HSSP:1PHP" FT /db_xref="InterPro:IPR015824" FT /db_xref="UniProtKB/Swiss-Prot:A5F9G2" FT /protein_id="AAC44768.1" FT /translation="MSVIKMIDLDLAGKRV" XX SQ Sequence 4407 BP; 1178 A; 1019 C; 1106 G; 1104 T; 0 other; atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct 60 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag 120 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga 180 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta 240 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc 300 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata 360 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc 420 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa 480 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg 540 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc 600 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca 660 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt 720 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga 780 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg 840 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca 900 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac 960 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa 1020 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt 1080 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta 1140 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat 1200 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg 1260 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca 1320 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta 1380 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg 1440 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg 1500 gcccgatgtc tacgctgtac ggctcggatg cgattggcgg cgtgatcaac atcatcactc 1560 gtaaagatca gcagcagtgg tcaggcaatg tgcagctatc gaccgtggta caagaaaatc 1620 gcgcctcagg cgatgaacaa agcgctaatt tcttcgtaac aggaccttta agtgatgctc 1680 tatcgctgca agtctacgga caaaccacgc aacgcgatga agacgaaatt gagcatggtt 1740 acggcgataa atcactgcgc agcttaacct caaagctcaa ctaccagctt aatccagatc 1800 atcaactgca actggaagcc ggtgtatcag cccaagatcg cgaaaataac gtgggcaagt 1860 cggcgcaatc cagcggatgt cgcggtacat gcagcaatac cgataaccag taccgtcgca 1920 accatgttgc cgtctctcac caaggggatt ggcagggtgt tgggcagtct gatacttatc 1980 tgcaatatga agagaacacc aataaatctc gtgagatgag cattgataat acggtgttca 2040 aatccacgtt agtggctcct attggtgagc atatgttgag ctttggtgtg gaaggtaaac 2100 acgaaagttt agaggataaa acctccaaca aaatttcgtc tcgcacgcat atctcgaaca 2160 ctcaatgggc gggattcatt gaagatgaat gggcgctcgc agagcaattc cgcctgacct 2220 ttggtggccg tctggatcat gataaaaact acggcagcca cttcagccca cgtgtgtatg 2280 gcgtatggaa tctcgatcct ctatggacag taaaaggtgg tgtttctact ggcttccgtg 2340 caccgcaatt acgtgaagtg acgccggatt gggggcaagt tagtggcggc ggtaatattt 2400 acggcaaccc ggatttacag cctgaaactt cgattaacaa agaactgagt ttgatgtaca 2460 gcactggctc aggtctggcg gcatcgttaa ccgctttcca taatgatttt aaagataaaa 2520 tcacccgcgt cgcttgccct gcaaatattt gtactgccgg ccccaaccaa tggggtgcaa 2580 ctcctactta tcgtgtgaat attgatgaag cggaaaccta tggtgcagaa gctacgttaa 2640 gtctgccgat taccgaaagc gttgagttat cgtctagcta cacgtacacc cattcggagc 2700 aaaaatcagg gaattttgct ggccgtccgc tactgcagtt acctaaacat ctgttcaatg 2760 ccaacttgag ctggcaaacc acagatcgcc tcaatagctg ggctaacctc aactatcgtg 2820 gtaaggagat gcagccggag ggtggggcat cgaacgatga tttcatcgcg ccaagctaca 2880 cctttatcga tactggcgtg acttacgcac tcaccgatac cgcaacgatt aaagctgcgg 2940 tgtacaacct gtttgatcaa gaggtgaatt acgcggagta tggctatgta gaagatggcc 3000 gccgctactg gttgggttta gacatcgcct tctaattgct agaggttcag aggattggcg 3060 gcagcgccaa tcctctgttt gttttgcgat cggcggtgga atcatttatt gcacaggatc 3120 gccgtgccaa agcctagagt atcggttact attgccaacc aatctgagag cgataggcga 3180 gatacgatgc tgagggtagc aattaacggc tttgggcgca ttgggcgtaa tgtgttacgc 3240 gctgtgtatg aaagtggtaa acgtgaccgc attcaagtgg ttgcggtgaa cgagcttgcc 3300 aagccagatg cgatggcaca cctcctgcag tacgatacga gccacggtcg ctttggcaaa 3360 aaaatcagcc atgatcagca gcatatctat gttcaccatc aaaatggtga gtatgactca 3420 atccgcattc tgcatctttc ggaaattcct ctcttacctt ggcgtgattt aggcgtggat 3480 ttagtcctcg actgcacagg tgtgtatggt tgccaagagg atggtcagca acatattgat 3540 gcgggcgcga agttagtttt attttcccat cctggtgcta gcgatctcga caacaccatc 3600 atctatggcg tgaatcatga gacgctcact gctgagcata aaatcgtctc taacggttct 3660 tgtaccacca actgcattgt gcccatcatc aaggtattgg atgacgcatt cggcattgat 3720 tctggcacca tcaccaccat ccattcctcg atgaacgatc aacaagtgat tgatgcgtat 3780 cataatgact tacgacgcac acgagcggcc agtcaatcta tcattccggt tgatactaag 3840 ttgcataaag gtattgaaag aatatttccg aaattttcta acaaatttga agcgatttcg 3900 gtgcgtgtac caacagtaaa tgtgaccgcg atggatttaa gtgtcacaat taaatcaaat 3960 gtgaaagtta atgacgtaaa tcaaaccatt gtgaacgcat ctcagtgtac attacgtggc 4020 attgttgact atactgaagc gcctttggtt tccatcgatt ttaaccacga tccgcacagc 4080 gcgattgtgg atgggacgca aacgagagtc agtaacgggc agttagtaaa aatgctcgtt 4140 tggtgtgata acgaatgggg ctttgccaac cggatgttag ataccgcatt ggcaatgcag 4200 gccacgcagt agcggctaag catgcacttc aaatcaggtt tgaacattga aacccgaagg 4260 acgcatcccc atatcggtga acagctaaga ttttaccgac tcggcagggt cgccgagttg 4320 cagaattttc atttctttaa atagtttgag aggacaaatc atgtctgtaa tcaagatgat 4380 tgacctggat cttgcaggta aacgcgt 4407 // |
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