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EBI DbfetchID U71377; SV 1; linear; genomic DNA; STD; PRO; 7485 BP. XX AC U71377; XX DT 22-JUL-1997 (Rel. 52, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Staphylococcus epidermidis autolysin AtlE and putative transcriptional DE regulator AtlR genes, complete cds. XX KW . XX OS Staphylococcus epidermidis OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-7485 RX DOI; 10.1046/j.1365-2958.1997.4101774.x RX PUBMED; 9220008. RA Heilmann C., Hussain M., Peters G., Gotz F.; RT "Evidence for autolysin-mediated primary attachment of Staphylococcus RT epidermidis to a polystyrene surface"; RL Mol. Microbiol. 24(5):1013-1024(1997). XX RN [2] RP 1-7485 RA Heilmann C., Wagner A., Goetz F.; RT ; RL Submitted (20-SEP-1996) to the EMBL/GenBank/DDBJ databases. RL Mikrobielle Genetik, Universitaet Tuebingen, Waldhaeuser Strasse 70/8, RL Tuebingen 72076, Germany XX FH Key Location/Qualifiers FH FT source 1..7485 FT /organism="Staphylococcus epidermidis" FT /strain="O-47" FT /mol_type="genomic DNA" FT /db_xref="taxon:1282" FT CDS <1..865 FT /codon_start=2 FT /transl_table=11 FT /note="ORF1" FT /db_xref="InterPro:IPR004474" FT /db_xref="UniProtKB/TrEMBL:O33632" FT /protein_id="AAB63568.1" FT /translation="SLHAYGGPLAAMDSVEATMNVPVDYYVRINMKAFVEAVDELGGIY FT YDVPYNLNEPNSDDTGRIKIKKGYQKLNGDQALAVARTRHHDSDLKRGQRQMELIKILF FT QKAQNLKSIDKLDNVISIVGKNAKHNLTQKEIKSLAKMYLGGSTEIKTSQLKGKDDYLN FT DIYYYHPSVKSIMEYSNLLRNDLDLSQITNKNDFLDQRVIKRYGSLVPLTELDEDLLRK FT NQKESTDSQKESDSSSQNNDEEDQTNEQTDQNSLNGNEQYPNQQDNNQTNGENGMINND FT NYPYAQ" FT CDS 913..1383 FT /codon_start=1 FT /transl_table=11 FT /note="ORF2" FT /db_xref="UniProtKB/TrEMBL:O33633" FT /protein_id="AAB63569.1" FT /translation="MSRKTYEKIANINGMFNVLEQQIIHSKDMALFRNEFFYVNHEHRE FT NYEALLIYYKDSLDNPVVDGACYILALPEIFNKVDVFESDLPFTWVYDENGLSDTMKSI FT SVPLQYLIAAALEVTDVNIFKPSGFTMGMNNWNIAQLRIFWQYCAIVRKEAL" FT CDS 1957..2379 FT /codon_start=1 FT /transl_table=11 FT /note="ORF3" FT /db_xref="GOA:O33634" FT /db_xref="HSSP:1Q2Y" FT /db_xref="InterPro:IPR000182" FT /db_xref="InterPro:IPR016181" FT /db_xref="UniProtKB/TrEMBL:O33634" FT /protein_id="AAB63570.1" FT /translation="MFQIAKNQTMLDDCFEIRKCVFVEEQGVPLENEFDQYEDYSFHIV FT GYINGVPMATARIRPLNTHICKIERVAIIKWYRGLGYGKNLIHAIETIAKKHQYNELTM FT NAQLQARDFYLKLGYSPFGKVFLEENINHISMNKFL" FT CDS 2620..6627 FT /codon_start=1 FT /transl_table=11 FT /product="autolysin AtlE" FT /note="primary attachment to a polystyrene surface" FT /db_xref="GOA:O33635" FT /db_xref="InterPro:IPR002502" FT /db_xref="InterPro:IPR002901" FT /db_xref="InterPro:IPR013338" FT /db_xref="UniProtKB/Swiss-Prot:O33635" FT /protein_id="AAB63571.1" FT /translation="MAKKFNYKLPSMVALTLFGTAFTAHQANAAEQPQNQSNHKNVLDD FT QTALKQAEKAKSEVTQSTTNVSGTQTYQDPTQVQPKQDTQSTTYDASLDEMSTYNEISS FT NQKQQSLSTDDANQNQTNSVTKNQQEETNDLTQEDKTSTDTNQLQETQSVAKENEKDLG FT ANANNEQQDKKMTASQPSENQAIETQTASNDNESQQKSQQVTSEQNETATPKVSNTNAS FT GYNFDYDDEDDDSSTDHLEPISLNNVNATSKQTTSYKYKEPAQRVTTNTVKKETASNQA FT TIDTKQFTPFSATAQPRTVYSVSSQKTSSLPKYTPKVNSSINNYIRKKNMKAPRIEEDY FT TSYFPKYGYRNGVGRPEGIVVHDTANDNSTIDGEIAFMKRNYTNAFVHAFVDGNRIIET FT APTDYLSWGAGPYGNQRFINVEIVHTHDYDSFARSMNNYADYAATQLQYYNLKPDSAEN FT DGRGTVWTHAAISNFLGGTDHADPHQYLRSHNYSYAELYDLIYEKYLIKTKQVAPWGTT FT STKPSQPSKPSGGTNNKLTVSANRGVAQIKPTNNGLYTTVYDSKGHKTDQVQKTLSVTK FT TATLGNNKFYLVEDYNSGKKYGWVKQGDVVYNTAKAPVKVNQTYNVKAGSTLYTVPWGT FT PKQVASKVSGTGNQTFKATKQQQIDKATYLYGTVNGKSGWISKYYLTTASKPSNPTKPS FT TNNQLTVTNNSGVAQINAKNSGLYTTVYDTKGKTTNQIQRTLSVTKAATLGDKKFYLVG FT DYNTGTNYGWVKQDEVIYNTAKSPVKINQTYNVKPGVKLHTVPWGTYNQVAGTVSGKGD FT QTFKATKQQQIDKATYLYGTVNGKSGWISKYYLTAPSKVQALSTQSTPAPKQVKPSTQT FT VNQIAQVKANNSGIRASVYDKTAKSGTKYANRTFLINKQRTQGNNTYVLLQDGTSNTPL FT GWVNINDVTTQNIGKQTQSIGKYSVKPTNNGLYSIAWGTKNQQLLAPNTLANQAFNASK FT AVYVGKDLYLYGTVNNRTGWIAAKDLIQNSTDAQSTPYNYTFVINNSKSYFYMDPTKAN FT RYSLKPYYEQTFTVIKQKNINGVKWYYGQLLDGKYVWIKSTDLVKEKIKYAYTGMTLNN FT AINIQSRLKYKPQVQNEPLKWSNANYSQIKNAMDTKRLANDSSLKYQFLRLDQPQYLSA FT QALNKLLKGKGVLENQGAAFSQAARKYGLNEIYLISHALVETGNGTSQLAKGGDVSKGK FT FTTKTGHKYHNVFGIGAFDNNALVDGIKYAKNAGWTSVSKAIIGGAKFIGNSYVKAGQN FT TLYKMRWNPANPGTHQYATDINWANVNAQVLKQFYDKIGEVGKYFEIPTYK" FT terminator 6633..6677 FT /note="transcriptional terminator" FT CDS complement(6867..7286) FT /codon_start=1 FT /transl_table=11 FT /product="putative transcriptional regulator AtlR" FT /db_xref="GOA:O33636" FT /db_xref="InterPro:IPR000835" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/TrEMBL:O33636" FT /protein_id="AAB63572.1" FT /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILYNYDR FT LSQYDLTMKISREQSIVSRWIKKLVQKGYIMSQQSHEDLRCKELILTEEARALIQQINT FT ARCELIEARCQCLSSDEIASLNQLLNKLNSRNIFI" XX SQ Sequence 7485 BP; 2872 A; 1240 C; 1147 G; 2226 T; 0 other; aagcttgcat gcatatggtg gacctcttgc tgctatggac tcagttgaag caacaatgaa 60 tgtaccggta gattattatg tgcgtattaa tatgaaagcc tttgttgaag cagttgatga 120 attaggtggt atatattatg acgtaccata taacttaaat gaacctaaca gtgatgatac 180 tggtagaatt aaaataaaaa aaggatacca aaagctaaac ggcgaccaag cattagctgt 240 agctcgaact agacaccatg attcagacct taaacgtggt caaagacaaa tggaacttat 300 taaaatattg ttccaaaaag ctcaaaattt aaaatctata gataaacttg acaatgttat 360 tagtattgta gggaaaaatg ctaaacataa tttaactcaa aaagaaatta agtctctagc 420 caaaatgtat cttggtggta gtactgaaat taaaacatca caacttaaag gtaaggatga 480 ctacttaaat gatatatact attaccaccc aagcgtaaaa agtattatgg aatattcaaa 540 tcttttacgt aatgatttag atttatctca aataacaaac aaaaacgatt tcttagatca 600 aagagtcatt aaacgatatg gttcactcgt acccttaaca gaattagatg aagacttatt 660 gcgtaagaac caaaaggaat cgactgatag tcagaaagag tctgattctt catcacaaaa 720 taatgatgaa gaagatcaaa ctaacgaaca aacagaccaa aatagcttaa acggaaacga 780 acagtaccca aatcaacaag acaacaatca aaccaatggt gaaaatggta tgataaataa 840 tgacaattat ccttacgcac aataaaagct atataaactt atgtagtaaa tatacttaaa 900 aggagcgcaa atatgagtcg taaaacatat gaaaaaattg ctaatattaa tggtatgttc 960 aacgtacttg aacaacaaat cattcatagt aaagatatgg cattatttag gaatgaattt 1020 ttctatgtaa accatgaaca cagagaaaat tatgaagcgc ttttgatcta ctacaaggat 1080 agtttagata atcccgttgt agatggcgca tgttacattc ttgcattacc tgaaatattt 1140 aataaggttg atgtatttga gtctgattta ccttttacct gggtttatga tgaaaatgga 1200 ctttctgata caatgaaaag tatcagtgtg ccccttcaat atcttattgc agccgctttg 1260 gaagtgactg atgtaaatat atttaagcct tcaggtttta ctatgggcat gaacaattgg 1320 aatattgccc aattacgtat tttttggcaa tactgcgcta tcgtcagaaa agaggcttta 1380 taatactatc aattagtaat aaacgtatcg tttcttaatt ctttggttta taaattatat 1440 atttttctaa ttatcagagt gaggttagga tataattcct agcaaaatag ccagtaaatg 1500 agttttttat aaattcattt actggcttct ttatttataa tacttcgtat tgttttctcg 1560 ctttcttagg ggacagcttc agcctgtagt cttcagcttg acctgttccc tcaagagtct 1620 cgccaaaata ctttatgttt atatgtaatt tcacattgaa atactttaaa aaataaggcc 1680 ctttcgtaaa atttaataaa tatcactaaa ataaattaac gaagttcctt atgtataaag 1740 attataataa gactcaactt actttaccaa tagaaacttc agttcttatc cccacaaatg 1800 atattcacga catgtacctg actcattctt tttttggtga tgaactaatt atgaatttat 1860 aatgaacctg ctttattcca ttttttataa attttcattt tcaatatggt taaaataatt 1920 ttttaaggaa aaaccagtga aaatggaggt gatactgtgt ttcaaatagc aaaaaaccaa 1980 acgatgctag atgattgctt tgaaataaga aagtgtgttt tcgtcgaaga acaaggcgta 2040 ccactcgaaa atgaatttga tcaatatgaa gattactcat tccatatagt gggatatata 2100 aatggtgttc ctatggcaac tgctagaatt agacctttaa atactcatat ttgtaaaatt 2160 gaacgtgtag caatcatcaa gtggtatcgt ggtcttgggt acggtaaaaa tttaatacat 2220 gctattgaaa caattgcaaa aaaacaccaa tacaatgaac tcactatgaa tgctcaatta 2280 caagctcgag acttttactt aaaactaggt tactcacctt ttggtaaagt attcttagaa 2340 gaaaatataa atcatattag tatgaataag tttttataaa ttgatttaca ttctatatac 2400 aaacttagca attttaaaaa ttgtaataat ctttatgtag cttacaagct tataataaac 2460 gtaaatagtg atttgacaat gttcccagca taaaaaaaca atatttctat ttgaggcaca 2520 ggttaaaatt acattacaaa tatgttaaaa gccatacagt gttaagcctt aaagattata 2580 attgaaacaa atcatattaa tgcgaggagt aagaaataaa tggcgaaaaa attcaattac 2640 aaattaccgt ctatggttgc tttaacgtta tttggcacag cttttactgc acatcaagca 2700 aatgctgctg aacaaccaca gaatcagtct aatcataaaa atgtattaga tgatcaaact 2760 gccctcaaac aagcagaaaa agctaaaagc gaagttacac aatcaactac aaatgtatct 2820 ggtacacaaa catatcaaga ccctacccaa gttcaaccta aacaagacac acaaagtact 2880 acatatgatg catcattaga tgaaatgagt acttataatg aaatttcatc aaatcaaaag 2940 caacaatctt tatcaacaga tgatgcgaat caaaatcaaa cgaattctgt tacaaaaaat 3000 caacaagaag aaacaaatga tttgacacaa gaagataaaa catccactga tacaaatcaa 3060 ttacaggaga cacaatctgt agcaaaagaa aatgagaaag atttaggagc taacgcaaat 3120 aatgaacaac aagacaagaa gatgactgca agtcaacctt ccgaaaatca agcaattgaa 3180 actcaaactg cttctaatga taatgaaagc caacaaaaaa gtcagcaagt aacttctgaa 3240 caaaatgaaa ctgctacacc taaagtatca aatacaaacg catctggtta taattttgat 3300 tacgatgatg aagacgatga tagctcaaca gaccatttag agcctatctc attaaacaat 3360 gtgaatgcta catctaaaca aactacttca tataaatata aagaaccagc tcaacgtgta 3420 acaactaata ctgtaaaaaa agaaacggca tctaatcaag cgactataga tacaaagcaa 3480 ttcaccccat ttagtgcaac tgctcaaccg agaacagttt attctgtatc tagtcaaaaa 3540 acatcatcat taccgaaata tacaccaaag gttaattctt caataaataa ctatattcgt 3600 aaaaagaata tgaaagcacc aagaattgaa gaagattata cgtcatattt ccctaaatat 3660 ggctatagaa acggtgtggg acgtcctgaa ggtatcgttg ttcatgatac tgcaaatgat 3720 aactcaacaa tcgatggcga gattgctttc atgaaacgta attacacaaa tgcattcgta 3780 cacgcatttg ttgatggcaa tagaattata gaaacagctc cgacagatta cttatcttgg 3840 ggtgcaggtc catatggaaa tcaacgtttt atcaatgttg aaatcgtcca tacacatgat 3900 tatgattcat ttgcacgttc aatgaacaac tacgctgatt atgctgcaac gcaattgcaa 3960 tattataatt taaaacctga tagcgctgaa aacgatggaa gaggaacagt ttggacacat 4020 gctgctatct ctaacttctt aggaggtact gatcacgctg accctcacca atatttaaga 4080 agtcacaatt atagctatgc agaattatat gacttaattt atgaaaaata tttaattaaa 4140 acgaagcaag tagcaccttg gggcacaaca tctacaaaac cgtcacaacc ttctaaacca 4200 tcaggaggaa ctaataataa attaactgtg tctgctaatc gtggtgttgc tcaaattaaa 4260 ccaacaaata atggcttata tacaactgtt tatgacagta aaggtcataa gactgatcaa 4320 gtacaaaaaa ctctatccgt tactaaaact gcaacattag gaaataacaa attctattta 4380 gttgaagact acaatagcgg taaaaaatac ggttgggtta aacaaggtga tgttgtttat 4440 aacactgcta aggcaccagt aaaagtgaat caaacatata atgttaaagc agggtcaaca 4500 ctttacacag ttccttgggg tacaccaaaa caagttgcta gcaaagtatc tggtactgga 4560 aatcaaacat ttaaagcaac taaacagcaa caaattgata aagcaacgta tctttatggt 4620 acagtgaatg gtaaatctgg ttggattagt aaatattact taactacagc atctaaacct 4680 agcaatccaa ctaaaccttc aacaaacaac caattaacag tgactaacaa tagtggtgtt 4740 gctcaaatca atgcaaaaaa tagtggctta tatactacag tttatgacac taaaggaaag 4800 acaacaaatc aaatccaacg tacattgtca gtgacgaaag ctgccacact tggtgataaa 4860 aaattctatc ttgttggtga ttataatact ggtacaaatt atggttgggt aaaacaagat 4920 gaggtcattt acaacacagc taaatcacct gtaaaaatca atcaaacata caacgtcaaa 4980 cctggtgtta aattacacac agtaccttgg ggcacatata atcaagtggc tggaacagtt 5040 tcaggtaaag gcgatcaaac ttttaaagca actaaacaac aacaaattga taaagcaaca 5100 tatctttatg gtacagtgaa cggtaaatct ggttggatta gtaaatacta tttaactgca 5160 ccatcaaaag ttcaagcttt gtctactcaa tcaacaccag cacctaaaca agtaaaacca 5220 tctacacaaa ctgtaaatca aattgctcaa gtgaaagcta ataattctgg aataagagca 5280 tctgtatatg ataaaacagc caaaagtggt acgaaatacg ctaaccgtac attccttatc 5340 aataaacaac gtactcaagg taataacacg tatgtactac ttcaagatgg aacaagtaat 5400 actccattag gatgggtaaa cattaatgat gtgacaactc aaaatatcgg aaaacaaact 5460 cagtctatag gtaaatattc agtaaaacct acaaataatg gtctatattc tattgcttgg 5520 ggtactaaaa accaacaatt actagcacct aatacgctag ctaatcaagc atttaatgct 5580 tccaaagctg tttacgttgg taaagattta tatctatacg gtacagtcaa taacagaaca 5640 ggatggattg ctgctaagga tttaatccaa aacagtactg acgctcaatc aacaccatat 5700 aactatactt ttgttatcaa taatagtaaa agttatttct atatggatcc aacaaaagca 5760 aaccgatatt ctttaaaacc atattatgaa caaactttca cagtcattaa gcaaaaaaat 5820 attaatggcg ttaaatggta ctatggtcaa cttttagacg gtaaatatgt ttggataaaa 5880 tcaactgact tagttaagga aaaaattaaa tatgcatata ctggaatgac tttaaataac 5940 gcgataaata tccaatctcg tcttaaatat aaaccacaag tacaaaatga gcctttgaaa 6000 tggtcaaatg ctaattatag tcaaattaaa aatgctatgg atacaaagcg tttagctaat 6060 gattcatcct taaaatatca attcttacgt ttagatcaac cacaatactt gtcagcacaa 6120 gctctcaata aattattaaa aggcaaaggt gtacttgaaa accaaggcgc tgcatttagc 6180 caagctgcac gtaagtatgg tctaaatgaa atttatctta tctcacatgc tttagtagaa 6240 acaggtaatg gaacttcaca acttgctaaa ggtggagatg tttcaaaagg taaattcaca 6300 actaaaacag gtcacaaata ccataatgtc tttggaattg gtgcatttga caataatgca 6360 cttgtagatg gtatcaaata cgctaaaaat gctggatgga cttctgtctc taaagcaatt 6420 attggtggcg ctaaattcat tggaaattca tacgtgaaag caggacaaaa tacgctatat 6480 aaaatgcgtt ggaatcctgc aaaccctggt acgcatcaat atgcaactga tattaattgg 6540 gcaaatgtca acgcacaagt attaaaacaa ttttatgata aaattggtga agtcggtaag 6600 tacttcgaaa ttccaacata caaataaata tgaagtaagg cagctctcat agttcatgaa 6660 agtagagctg cctacttttt tgatgattat ttatcctgtc actgcttcga atatgacata 6720 tgctaaatat taaacttttt caaatataaa aaagctaagc acacttatat gtaatttaat 6780 attacaattc agttgcttag ctttatatgt ttatatataa ttgaatgtat tttacactta 6840 acatcataat taaaaaccga tatatatcag atgaaaatgt ttctactatt aagtttgttt 6900 aataattgat ttaaacttgc aatttcgtct gaagatagac attgacatct cgcttcaatt 6960 aactcgcatc tcgccgtgtt gatttgctga attaaagccc ttgcttcttc agttaatatc 7020 agttctttac atcgtaaatc ttcatgagat tgttgactca tgatataacc tttttggact 7080 aactttttaa tccatcttga tacaattgat tgttccctac taatcttcat agttaaatcg 7140 tattgagata aacgatcata attatataaa atacgtagta actctaattg ttcagaagta 7200 atgtcagttc gttgaccaaa gcgtctatta actaaattca aatcattatt tgtcagtgtg 7260 ataagttttt caagttgttt atacatgttg ttctactcca ctatattaag ttaataatat 7320 tatatgcctt atttataaat tatacaatac aaatatacac attgcacaaa ttcgttgcat 7380 cattacctat ttttatacta cattagagta tgtctaaatt gtaaaaaagg agagcgttta 7440 tgacagccat caaagatagg gattattttt tcgataatgc tcgac 7485 // |
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