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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   U65000; SV 1; linear; genomic DNA; STD; PRO; 1220 BP.
XX
AC   U65000;
XX
DT   06-OCT-1996 (Rel. 49, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   Staphylococcus aureus type-I signal peptidase SpsA (spsA) gene, and type-I
DE   signal peptidase SpsB (spsB) gene, complete cds.
XX
KW   .
XX
OS   Staphylococcus aureus
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
XX
RN   [1]
RP   1-1220
RX   PUBMED; 8824617.
RA   Cregg K.M., Wilding E.I., Black M.T.;
RT   "Molecular cloning and expression of the spsB gene encoding an essential
RT   type I signal peptidase from Staphylococcus aureus";
RL   J. Bacteriol. 178(19):5712-5718(1996).
XX
RN   [2]
RP   1-1220
RA   Cregg K.M., Wilding E.I., Black M.T.;
RT   ;
RL   Submitted (24-JUL-1996) to the EMBL/GenBank/DDBJ databases.
RL   Biotechnology, SmithKline Beecham, Yew Tree Bottom Road, Epsom, Surrey KT18
RL   5XQ, UK
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1220
FT                   /organism="Staphylococcus aureus"
FT                   /strain="WCUH29"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1280"
FT   RBS             28..32
FT   CDS             40..564
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="spsA"
FT                   /product="type-I signal peptidase SpsA"
FT                   /note="inactive signal peptidase homologue; protein lacks
FT                   catalytic elements of bacterial type-I signal peptidases
FT                   but is homologous"
FT                   /db_xref="GOA:P0A066"
FT                   /db_xref="InterPro:IPR019759"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A066"
FT                   /protein_id="AAC44434.1"
FT                   /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGDR
FT                   VIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDASYA
FT                   KNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISLRYY
FT                   PFSKWTIQFKS"
FT   RBS             567..571
FT   CDS             580..1155
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="spsB"
FT                   /product="type-I signal peptidase SpsB"
FT                   /note="signal peptidase, leader peptidase, serine protease"
FT                   /db_xref="GOA:P0A070"
FT                   /db_xref="HSSP:1B12"
FT                   /db_xref="InterPro:IPR011056"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A070"
FT                   /protein_id="AAC44435.1"
FT                   /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDGE
FT                   RVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQDEP
FT                   YLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGLIDE
FT                   DQIVGKVSFRFWPFSEFKHNFNPENTKN"
FT   terminator      1170..1207
XX
SQ   Sequence 1220 BP; 443 A; 154 C; 221 G; 402 T; 0 other;
     tagaacagca ttttatggga tcgaaaaagg agtgacatcg tgaaaaaagt tgtaaaatat        60
     ttgatttcat tgatacttgc tattatcatt gtactgttcg tacaaacttt tgtaatagtt       120
     ggtcatgtca ttccgaataa tgatatgtcg ccaaccctta acaaagggga tcgtgttatt       180
     gtaaataaaa ttaaagttac atttaatcaa ttgaataatg gtgatatcat tacatatagg       240
     cgtggtaacg agatatatac tagtcgaatt attgccaaac ctggtcaatc aatggcgttt       300
     cgtcagggac aattataccg tgatgaccga ccggttgacg catcttatgc caagaacaga       360
     aaaattaaag attttagttt gcgcaatttt aaagaattag atggagatat tataccgcct       420
     aacaattttg ttgtgctaaa tgatcatgat aacaatcagc atgattctag acaatttggt       480
     ttaattgata aaaaggatat tattggtaat ataagtttga gatattatcc tttttcaaaa       540
     tggacgattc agttcaaatc ttaaaaagag gtgtcaaaat tgaaaaaaga attattggaa       600
     tggattattt caattgcagt cgcttttgtc attttattta tagtaggtaa atttattgtt       660
     acaccatata caattaaagg tgaatcaatg gatccaactt tgaaagatgg cgagcgagta       720
     gctgtaaaca ttattggata taaaacaggt ggtttggaaa aaggtaatgt agttgtcttc       780
     catgcaaaca aaaatgatga ctatgttaaa cgtgtcatcg gtgttcctgg tgataaagta       840
     gaatataaaa atgatacatt atatgtcaat ggtaaaaaac aagatgaacc atatttaaac       900
     tataatttaa aacataaaca aggtgattac attactggga ctttccaagt taaagattta       960
     ccgaatgcga atcctaaatc aaatgtcatt ccaaaaggta aatatttagt tcttggagat      1020
     aatcgtgaag taagtaaaga tagccgtgcg tttggcctca ttgatgaaga ccaaattgtt      1080
     ggtaaagttt catttagatt ctggccattt agtgaattta aacataattt caatcctgaa      1140
     aatactaaaa attaatatga aacaaataca acatcgtttg tcggttttaa tactgataaa      1200
     cgatgtttta ttttgttagt                                                  1220
//
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