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EBI DbfetchID U63302; SV 1; linear; genomic DNA; STD; PRO; 5100 BP. XX AC U63302; S61781; U33938; XX DT 04-JUL-1997 (Rel. 52, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Bacillus subtilis putative aminotransferase (patB), ATP-dependent protein DE kinase B (kinB), kinase-associated protein B (kapB) and KapD (kapD) genes, DE complete cds. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3771 RX DOI; 10.1111/j.1365-2958.1993.tb01204.x RX PUBMED; 8497199. RA Trach K.A., Hoch J.A.; RT "Multisensory activation of the phosphorelay initiating sporulation in RT Bacillus subtilis: identification and sequence of the protein kinase of the RT alternate pathway"; RL Mol. Microbiol. 8(1):69-79(1993). XX RN [2] RP 2849-5100 RX PUBMED; 7961491. RA Popham D.L., Setlow P.; RT "Cloning, nucleotide sequence, mutagenesis, and mapping of the Bacillus RT subtilis pbpD gene, which codes for penicillin-binding protein 4"; RL J. Bacteriol. 176(23):7197-7205(1994). XX RN [3] RP 2849-5100 RA Dartois V.A., Grau R., Liu J., Hoch J.A.; RT "Characterization of the kapD gene and encoded protein which specifically RT inhibits the KinA pathway to sporulation in Bacillus subtilis"; RL Unpublished. XX RN [4] RP 1-5100 RA Dartois V.A., Grau R., Liu J., Hoch J.A.; RT ; RL Submitted (09-JUL-1996) to the EMBL/GenBank/DDBJ databases. RL Molecular and Experimental Medicine, Scripps Research Institute, 10666 RL North Torrey Pines Road, La Jolla, CA 92037, USA XX CC On Jul 2, 1997 this sequence version replaced gi:975349. XX FH Key Location/Qualifiers FH FT source 1..5100 FT /organism="Bacillus subtilis" FT /map="285 degree" FT /strain="168" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS <1..219 FT /codon_start=1 FT /transl_table=11 FT /note="putative ORF" FT /db_xref="UniProtKB/TrEMBL:O05230" FT /protein_id="AAB61978.1" FT /translation="PPGDRQRLFKRSFKYLLNAFRQQVGIVFIIDCLHDINSLRFVKLF FT SIFKRVPFGNDLNHFPDRMTFFHIHRL" FT CDS 288..1451 FT /codon_start=1 FT /transl_table=11 FT /gene="patB" FT /product="PatB" FT /note="putative aminotransferase" FT /db_xref="GOA:Q08432" FT /db_xref="HSSP:1C7N" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:Q08432" FT /protein_id="AAB61979.1" FT /translation="MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAI FT TEALKERLDHGIFGYTTPDQKTKDAVCGWMQNRHGWKVNPESITFSPGVVTALSMAVQA FT FTEPGDQVVVQPPVYTPFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPSVTL FT FILCNPHNPSGRSWSREDLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDF FT ADISVTCAAPSKTFNIAGLQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAY FT SKGGPWLDELITYIEKNMNEAEAFLSTELPKVKMMKPDASYLIWLDFSAYGLSDAELQQ FT RMLKKGKVILEPGTKYGPGGEGFMRLNAGCSLATLQDGLRRIKAALS" FT CDS 1577..2863 FT /codon_start=1 FT /transl_table=11 FT /gene="kinB" FT /product="KinB" FT /function="sensor kinase activating the alternate pathway FT to sporulation in B. subtilis" FT /note="ATP-dependent protein kinase B" FT /db_xref="GOA:Q08430" FT /db_xref="HSSP:1I59" FT /db_xref="InterPro:IPR005467" FT /db_xref="UniProtKB/Swiss-Prot:Q08430" FT /protein_id="AAB61980.1" FT /translation="MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFA FT CGASVLCIIFPIHEMDYIQYGLQMIPVIICLFYISTASGLTVAASVLCFELLFYEPSAM FT FVFTLLPFLIIIPILFQKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNILNFQ FT KSGIFVYEAAVSGLFRSSVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHE FT VRNPLTVVRGFVQLLFNDETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKE FT VFDLSALIKETSSLMVSYANYKSVTVEAETEPDLLIYGDATKLKQAVINLMKNSIEAVP FT HGKGMIHISAKRNGHTIMINITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSII FT EHHHGTISFNSSFQKGTTVTIKLPADLPH" FT CDS 2909..3295 FT /codon_start=1 FT /transl_table=11 FT /gene="kapB" FT /product="kinase-associated protein B" FT /function="required for activation of kinase B in the FT initiation of sporulation" FT /note="second and last ORF of the kinB-kapB operon; FT lipoprotein" FT /db_xref="GOA:Q08429" FT /db_xref="InterPro:IPR014916" FT /db_xref="UniProtKB/Swiss-Prot:Q08429" FT /protein_id="AAB61981.1" FT /translation="MSTFETGSIVKGFYKTGVYIGEITACRPQHYLVKVKAVLTHPAQG FT DLHHPKQADVPFFHERKALAYGEQTNIPHHMVKPYDGEVPDYTESLREATAQMRAKLNE FT DGSEWAKRSLHNLDILEKEYFNRP" FT CDS complement(3322..3939) FT /codon_start=1 FT /transl_table=11 FT /gene="kapD" FT /product="KapD" FT /function="inhibitor of the KinA pathway to sporulation" FT /db_xref="GOA:O05231" FT /db_xref="InterPro:IPR006055" FT /db_xref="UniProtKB/Swiss-Prot:O05231" FT /protein_id="AAB61982.1" FT /translation="MTTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVET FT FSSYVRPKKFPKLTKRCKSFLKITQKQVDEGMRFEDFIRKLNELDPEKNSTIITWGNMD FT MKVLKQNCMFNHIPFPFKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHHKAH FT DDALTAYKLFKLVEQDKQYLEKPKPPTIGERIDLTELLKRAT" FT CDS 4270..>5100 FT /codon_start=1 FT /transl_table=11 FT /gene="orf2" FT /product="ORF2" FT /note="Putative integral membrane protein; similar to FT multi-drug resistance transporters" FT /db_xref="InterPro:IPR011701" FT /db_xref="UniProtKB/TrEMBL:O24686" FT /protein_id="AAB61983.1" FT /translation="MFGITFLMAFLVSPFWGRFGDKRDTRKSLWQPEQHCPQHFLHGLC FT YIGVPIVFSAYGNGSVTGFIPTSLAMISAQTPKSSAGKTLGTLQMGQVSGSLFGPLLGG FT MLADRFGFTYTFFITSFVIFSSVLLVLFGVKEKHLAEKTAKRTSYSRKEVLSYIFHHPA FT LWVMMLLTMLIQTGNFSIQPLLALYVNELHGPVNLAFFSGMAFSATGLGSLLLARKWGD FT LGDRYGHRRILIGLLLAASFFFIPQALASSLSVLLVFRFLFGMAMGGLLPCITAA" XX SQ Sequence 5100 BP; 1340 A; 1167 C; 1099 G; 1494 T; 0 other; ccccctggcg atcggcagcg tttgttcaaa agaagtttca aatatttgct gaatgctttt 60 cgccagcaag tcgggatcgt tttcatcata gactgcctgc acgacatcaa cagcctccgt 120 ttcgtaaaac tcttctccat atttaaacgg gtcccatttg gcaatgatct taatcatttc 180 cctgaccgca tgactttctt ccatattcac cgcttataat aaaatttgca gttctatctt 240 atcatagtga agcttagaag aataaacata catgaggggg taacagaatg aactttgata 300 aacgagaaga acgccttggc acccaatcgg tcaaatggga taaaacaggc gaattatttg 360 gcgtgacaga cgccctcccg atgtgggtgg cagatatgga ttttcgcgcc ccggaagcga 420 taactgaagc gttaaaagag cgtcttgacc acggtatttt tggttataca actccagatc 480 aaaaaacgaa ggatgctgtg tgcggatgga tgcaaaacag gcacggctgg aaggtaaatc 540 cggaaagcat cacattcagc cccggcgttg taacagcttt gagcatggcg gtacaagctt 600 ttacggagcc gggcgatcaa gtagttgttc agcctccagt ttatacgcct ttttaccata 660 tggtggagaa aaacggccgg cacattttac ataatccgct gctagaaaaa gacggcgcat 720 atgcaatcga ttttgaagat ttggagacaa agcttagtga cccaagcgtt acattgttta 780 ttttatgcaa tcctcataac ccttctgggc gttcatggag ccgggaagat ttactgaagc 840 taggcgaatt gtgccttgag catggcgtca cagttgtatc ggatgaaatc cattccgatt 900 taatgctgta cggacataaa cacacgccgt tcgcttcgct ctctgacgat ttcgctgata 960 tttccgtgac atgtgctgct ccaagcaaaa catttaatat cgccggattg caggcttcag 1020 cgatcattat tccggatcgc ctgaagcgcg ccaagttttc cgcaagcctt cagcgaaatg 1080 gtttaggcgg actgaacgcg tttgccgtca ctgcaatcga agccgcgtat tcaaaaggcg 1140 gaccgtggct tgatgaattg atcacttaca ttgagaaaaa catgaacgaa gcggaagcct 1200 ttttgagcac cgagctgcca aaggtcaaaa tgatgaagcc ggatgcatca tacttgattt 1260 ggctcgactt cagcgcttac ggcttgtccg atgcagagct tcagcaaaga atgctaaaaa 1320 aaggaaaagt tattttagag cccgggacga aatacgggcc tggcggagaa ggatttatgc 1380 gcctgaacgc cggatgctct cttgcaaccc tgcaggacgg cctgcgccgc atcaaagccg 1440 cattatcgta aatatagctg taaacgcctt tacgtcttca tttgtaaagg cgttcttaat 1500 aaaggaattt tatattttac ttctaatatt tagtgttata atagattcat attttatacg 1560 aaaggatttc acacgaatgg aaattctaaa agactatctt ctgcacatct gctttatttt 1620 gtttcctatt cttctttacc aagtgttttg gcttggaaaa cctgcgattc ttgtgccaaa 1680 aataaacagc ggtttggtca cgctcttcgc ctgcggcgcc tccgttttat gcattatttt 1740 cccgattcat gaaatggact acatacaata cggccttcag atgattcccg ttatcatttg 1800 tttattttac attagtaccg cttccggact tacagtcgcc gcatctgtcc tatgttttga 1860 actgcttttt tacgaacctt ccgcaatgtt tgttttcact ttgctgcctt ttcttatcat 1920 tatcccaatc ttatttcaga aaaaatggcc attcatgtcc aaagcgaaaa aactcctgct 1980 gtctttattg atcagctgcg tggagatttt cctatttttc gcctcaagct ggattctttc 2040 cgccttgaat attttaaatt ttcaaaaatc aggaattttc gtttacgagg cggcagtctc 2100 agggcttttc cgctccagtg ttcttctatt aagcatctat attatcgaaa gcatcgccga 2160 aaatatcgcc ctgcgttcac agcttatcca ttctgaaaaa atgacgattg tgagtgaact 2220 ggcagcgagc gtcgcccatg aggtcaggaa tccgctgaca gttgtccgcg ggtttgtcca 2280 gctgcttttt aatgacgaaa ccctacaaaa caagtcgagt gcggattaca aaaagcttgt 2340 actgtcagag cttgaccggg cgcaaggcat tatcacaaac tatctcgata tggccaaaca 2400 gcaattatac gaaaaagagg tttttgacct atctgccctt atcaaagaga caagctccct 2460 tatggtgtcg tacgccaatt acaagtcagt aaccgtcgag gctgaaacag aaccggacct 2520 tttgatatac ggagacgcaa caaagctgaa gcaggctgtc atcaacctga tgaaaaacag 2580 tattgaagcg gttcctcacg ggaaggggat gatccacata tcagccaaaa gaaacggcca 2640 tacgatcatg attaacatca cagacaatgg ggtcgggatg acagaccatc aaatgcaaaa 2700 actcggcgaa ccttattatt ccttaaaaac gaacggaacg ggactcggcc tcaccgtaac 2760 cttttccatt attgaacatc atcacgggac catttcattt aacagcagct tccaaaaagg 2820 cactacggtg accattaagc ttcccgctga tcttcctcac tagcaaatcg attggaactt 2880 taatagaata aatgcgaggt gatgacagat gagcacgttt gagacaggct ctatcgttaa 2940 aggattttac aaaacaggtg tatacatagg agaaatcacg gcttgcaggc cgcagcatta 3000 tttagtgaaa gtgaaagcgg ttcttaccca tcccgctcag ggagaccttc atcatccaaa 3060 acaggcggat gtgccatttt ttcatgagag aaaagcgctc gcatatggtg aacagaccaa 3120 tatcccccac catatggtga agccttatga cggtgaagtg ccggattata ccgaatctct 3180 gcgggaggcg acagcacaga tgagagccaa attgaatgag gatggttcgg agtgggcaaa 3240 gcgctccctt cataatctgg acattttaga aaaagaatat tttaaccggc cataacccag 3300 tctgacaaaa gattgggttt tttacgtcgc gcgcttcaac agttcggtta aatcgattct 3360 ctcgccgatt gtgggcggtt ttggcttttc gagatactgc ttatcctgct caacaagctt 3420 aaaaagttta tatgctgtga gagcatcatc atgcgcttta tgatgggtgc cggttccgga 3480 atctccatac tcttcggcag ctttccataa acccgtcaat gtccggtctc caaaaaagtt 3540 tttgtattca agcgataaat ctctcatttc tcccttaaac gggaaaggga tatggttaaa 3600 catacagttt tgcttcagca ctttcatatc catgtttccc cacgtaataa ttgtgctgtt 3660 tttctcagga tccagctcgt ttagtttacg gatgaaatcc tcgaatctca ttccttcgtc 3720 tacctgtttt tgggttattt ttaaaaatga tttgcagcgt ttcgtaagct tcgggaactt 3780 tttcggtcta acatagctag aaaacgtctc aaccacttca tcatcaatcg actttacaat 3840 tccggcttca atgatttccg ggaagaagtt ttgaggactg tactttccat caggcattgt 3900 aaattcgaaa tcaataatga gtaagctgtt tgtcgtcacc ttttacacct gcctttcctc 3960 cgatcagaaa atgctatctt tatagtatat caattcccct tctcgacatt tcttcatttt 4020 ctaaaaataa tgaattttcc cagccgcttc gtttctttta aattgtgaat cgtttcttta 4080 taattagtaa ggaacccgaa ataataagaa ggtgatgtta tttctatccg taagaaaaac 4140 tttgtcattg tgtggctggc gaactttttt gtctccgcta gcacaacgat gatcgttcct 4200 tttctctcct tatacattga gacgctcagc tctttttcaa acggctttgt gcaaaggtgg 4260 agcggctatg tgttcggcat tacatttctc atggcgtttt tggtatcccc gttttgggga 4320 cggttcggag acaaacgcga tacaagaaaa tccttatggc aaccggaaca gcattgccct 4380 cagcattttc ttcatgggct ttgttacatc ggtgtaccaa ttgttttttc tgcgtatggc 4440 aatgggtctg taaccggatt catcccgaca tctcttgcga tgatttcggc acagactccg 4500 aagagctcag ctggcaaaac actcggcacg ctgcaaatgg ggcaagtgtc cggaagcttg 4560 ttcggcccgc ttttgggagg catgcttgct gatcgtttcg gctttacgta tacctttttt 4620 atcacatcct ttgtgatttt ctcgtctgtt cttctcgtgt tatttggtgt caaagaaaag 4680 catcttgcag agaaaacagc caaacgtacg tcttactcga gaaaagaagt gctctcctat 4740 atttttcacc atccggcgct gtgggtcatg atgctgttaa ccatgctgat tcaaacaggc 4800 aatttcagta tccagcccct gctcgcttta tatgtaaatg agctgcacgg acctgtcaat 4860 cttgcctttt tctccggcat ggcattttca gcaacggggc tcggaagttt gctgctggct 4920 agaaaatggg gtgatctggg cgaccgctac ggccaccgcc gcattttaat cggacttttg 4980 ctcgccgcct ctttcttttt tattccgcag gcgctcgcgt cctcactcag cgtgctcctt 5040 gttttcagat ttttattcgg aatggcgatg ggcggtttgc tgccatgcat tacggcagcg 5100 // |
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