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EBI DbfetchID U59303; SV 1; linear; genomic DNA; STD; FUN; 2421 BP. XX AC U59303; XX DT 28-JUN-1996 (Rel. 48, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE Aspergillus awamori glucoamylase precursor (gla) gene, complete cds. XX KW . XX OS Aspergillus awamori OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; OC Aspergillus. XX RN [1] RP 1-2421 RA Diagne I., Pechexonov V.T., Bulat S.A., Firsov L.M.; RT "A glucoamylase gene from Aspergillus awamori X-100: structure, allocation, RT and gene phylogeny"; RL Unpublished. XX RN [2] RP 1-2421 RA Diagne I.; RT ; RL Submitted (24-MAY-1996) to the EMBL/GenBank/DDBJ databases. RL Petersburg Nuclear Physics Institute, Russian Academy of Science, Molecular RL and Radiation Biophysics Division, Orlova Rocha, Gatchina, Leningrad RL District, 188350, Russia XX FH Key Location/Qualifiers FH FT source 1..2421 FT /organism="Aspergillus awamori" FT /strain="X-100" FT /mol_type="genomic DNA" FT /db_xref="taxon:105351" FT CAAT_signal 27..30 FT /gene="gla" FT TATA_signal 97..102 FT /gene="gla" FT CDS join(215..428,507..790,846..942,1005..1642,1701..2387) FT /codon_start=1 FT /gene="gla" FT /product="glucoamylase precursor" FT /EC_number="3.2.1.3" FT /note="1,4 alpha-D glucan glucano hydrolase precursor" FT /db_xref="GOA:Q12537" FT /db_xref="HSSP:1GAI" FT /db_xref="InterPro:IPR018247" FT /db_xref="UniProtKB/TrEMBL:Q12537" FT /protein_id="AAB02927.1" FT /translation="MSFRSLLALSGLVCSGLASVISKRATLDSWLSNEATVARTAILNN FT IGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGLVIKTLVDLFRNGDTDLLSTIEH FT YISSQAIIQGVSNPSGDLSSGGLGEPKFNVDETAYTGSWGRPQRDGPALRATAMIGFGQ FT WLLDNGYTSAATEIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRALVEG FT SAFATAVGSSCSWCDSQAPQILCYLQSFSSRSGWTGSYILANFDKDTNTLLGSIHTFDP FT EAGCDDSTFQPCSPRALANHKEVVDSFRSIYTLNDGLSDSEAVAVGRYPEDSYYNGNPW FT FLCTLAAAEQLYDALYQWDKQGSLEITDVSLDFFKALYSGAATGTYSSSSSTYSSIVSA FT VKTFADGFVSIVETHAASNGSLSEQFDKSDGDELSARDLTWSYAALLTANNRRNSVVPP FT SWGETSASSWPGTCAATSASGTYSSVTVTSWPSIVATGATTTTATTTGSGGVTSTSKTT FT TTASKTSTTTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWETSDGIALSA FT DKYTSSNPLWYVTVTLPAGESFEYKFIRVESDDSVEWESDPNREYTVPQACGESTATVT FT DTWR" FT exon <215..428 FT /gene="gla" FT /number=1 FT sig_peptide 215..286 FT /gene="gla" FT mat_peptide join(287..428,507..790,846..942,1005..1642,1701..2384) FT /gene="gla" FT /product="glucoamylase" FT /EC_number="3.2.1.3" FT /note="1,4 alpha-D glucan glucano hydrolase" FT intron 429..506 FT /gene="gla" FT /number=1 FT misc_signal 477..482 FT /gene="gla" FT /note="PuCTPuAC; Pu-purin consensus splicing signal" FT exon 507..790 FT /gene="gla" FT /number=2 FT intron 791..845 FT /gene="gla" FT /number=2 FT misc_signal 833..838 FT /gene="gla" FT /note="PuCTPuAC; Pu-purin consensus splicing signal" FT exon 846..942 FT /gene="gla" FT /number=3 FT intron 943..1004 FT /gene="gla" FT /number=3 FT misc_signal 986..991 FT /gene="gla" FT /note="PuCTPuAC; Pu-purin consensus splicing signal" FT exon 1005..1642 FT /gene="gla" FT /number=4 FT intron 1643..1700 FT /gene="gla" FT /number=4 FT misc_signal 1687..1692 FT /gene="gla" FT /note="PuCTPuAC; Pu-purin consensus splicing signal" FT exon 1701..>2387 FT /gene="gla" FT /number=5 XX SQ Sequence 2421 BP; 469 A; 683 C; 657 G; 612 T; 0 other; gctagatgtt aagcgatgtt gcattgcaat ttgtattgtg tgttgatgca tgtgcttctt 60 ccttcaactt ccccctcgtg cagatgttgg tttggatata aattgacgtg gtcgctcagg 120 ggtcagtgag gggttgaaag tagcttcctc cccctcaaga cgcgacaact gagtgactga 180 gcttcatcct caacatcgtt actcccccac cgcaatgtcg ttccgatctc ttctcgccct 240 gagcggcctt gtctgctctg ggttggcaag tgtgatttcc aagcgcgcga ccttggattc 300 gtggttgagc aacgaagcga ccgtggctcg tactgcgatc ctgaataaca tcggggcgga 360 cggtgcttgg gtgtcgggcg cggactctgg cattgtcgtt gccagtccca gcaccgataa 420 cccggactgt atgttttgag ttcggattat gaatgtgtct tggttgattg attgatgctg 480 actggcgtgt cttttgatga ttgtagactt ctacacctgg actcgcgact ctggtctcgt 540 catcaagacc ctcgtcgacc tcttccgcaa tggagatact gatctccttt ccaccattga 600 gcactacatc tcctctcagg caattattca gggtgtcagt aacccctctg gtgatctgtc 660 cagcggtggt cttggtgagc ccaagttcaa tgtcgatgag actgcctaca ccggttcttg 720 gggacggccg cagcgtgatg gtcctgccct gagagcaact gctatgatcg gctttgggca 780 gtggctgctt gtatgttctc cacccccttg cgtctgatct gcaacatatg tagctgactg 840 gtcaggacaa tggctacacc agcgctgcaa cagagattgt ttggcccctc gttaggaacg 900 acctgtcgta tgtggctcag tactggaacc agacgggata tggtgtgttt gattgatcgg 960 ggttcaaggg tgtttgtgca tcggagctaa cttcgcggtc gcagatctct gggaagaagt 1020 taatggctcg tccttcttca ctattgccgt gcaacaccgc gccctcgtcg aaggtagtgc 1080 cttcgcgacg gccgtcggct cgtcctgctc ctggtgtgat tcgcaggcac ctcagattct 1140 ctgttacttg cagtccttca gcagccgttc cggctggacc ggcagctaca tcctggccaa 1200 ctttgacaag gacacaaaca ccctcctggg aagcatccac acctttgatc ctgaggctgg 1260 atgcgacgac tccaccttcc agccctgctc cccgcgtgcg ctcgccaacc ataaggaggt 1320 tgtagactct ttccgctcga tctatactct caacgatggt ctcagtgaca gtgaggcggt 1380 tgcggtcggt cggtaccctg aggatagcta ctacaacggc aacccgtggt tcctgtgcac 1440 cttggctgcc gcggaacagc tgtacgatgc tctgtaccag tgggacaagc aggggtcgtt 1500 ggagatcaca gacgtgtcac ttgacttctt caaggctctg tacagtggtg ctgccaccgg 1560 cacgtactct tcgtccagct cgacctatag cagcattgtg agtgccgtca agactttcgc 1620 tgatggtttt gtttctattg tggtaagtct acgctagacg agcgctcata tttacagagg 1680 gtgcgtacta acagaattag gaaactcacg ccgcaagcaa cggctctctg tctgagcaat 1740 tcgacaagtc tgatggcgac gagctttctg ctcgcgatct gacctggtct tacgctgctc 1800 tgctgaccgc caacaaccgt cgtaattctg tcgtgccccc gtcttggggt gagacctctg 1860 ccagcagctg gcccggcacc tgtgcggcta cctctgcctc tggtacctac agcagtgtga 1920 ccgtcacctc gtggccgagc atcgtggcta ctggtgccac cactacgacg gctactacca 1980 ctggatcggg cggcgtgacc tcgaccagca agaccaccac aactgctagt aagaccagca 2040 ccactacgtc ctcgacctcc tgcaccaccc ccactgccgt agctgtgacc tttgatctga 2100 cggcgaccac cacctacggc gagaacatct acctggtcgg gtcgatctct cagctcggtg 2160 actgggagac cagcgatggc atagctctga gcgctgacaa gtacacttcc agtaacccgc 2220 tttggtatgt aactgtgact ctgccggctg gtgagtcatt tgagtacaag ttcatccgcg 2280 ttgagagcga tgactccgtg gagtgggaga gcgacccgaa ccgggaatac accgttcctc 2340 aggcgtgcgg cgagtcgacc gcgacggtga ccgacacctg gcggtagact attcaatcct 2400 attgatatga aggatgagta t 2421 // |
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