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EBI DbfetchID U56424; SV 1; linear; genomic DNA; STD; PRO; 3152 BP. XX AC U56424; XX DT 31-MAY-1996 (Rel. 47, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 8) XX DE Clostridium stercorarium cellobiose-phosphorylase (cepA) gene, complete DE cds. XX KW . XX OS Clostridium stercorarium OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3152 RX DOI; 10.1111/j.1432-1033.1997.00262.x RX PUBMED; 9249035. RA Reichenbecher M., Lottspeich F., Bronnenmeier K.; RT "Purification and properties of a cellobiose phosphorylase (CepA) and a RT cellodextrin phosphorylase (CepB) from the cellulolytic thermophile RT Clostridium stercorarium"; RL Eur. J. Biochem. 247(1):262-267(1997). XX RN [2] RP 1-3152 RA Reichenbecher M., Staudenbauer W.L.; RT ; RL Submitted (24-APR-1996) to the EMBL/GenBank/DDBJ databases. RL Marisa Reichenbecher, Technical University of Muenchen, Institute for RL Microbiology, Arcisstr 16, Muenchen D-80290, Germany XX FH Key Location/Qualifiers FH FT source 1..3152 FT /organism="Clostridium stercorarium" FT /strain="NCIB 11754" FT /mol_type="genomic DNA" FT /db_xref="taxon:1510" FT -35_signal 147..152 FT -10_signal 171..176 FT RBS 180..184 FT /gene="cepA" FT CDS 192..2627 FT /codon_start=1 FT /transl_table=11 FT /gene="cepA" FT /product="cellobiose-phosphorylase" FT /function="phosphorolysis of beta-1,4 linkages of FT cellobiose" FT /EC_number="2.4.1.20" FT /note="similar to SwissProt Accession Number P20471 and FT GenBank Accession Number U24658" FT /db_xref="GOA:Q59316" FT /db_xref="InterPro:IPR009342" FT /db_xref="UniProtKB/TrEMBL:Q59316" FT /protein_id="AAC45510.1" FT /translation="MKFGYFDDVNREYVITTPATPYPWINYLGCQDFFSLISNTSGGYC FT FYRDARLRRITRYRYNNVPIDSGGRYFYIYDSGDYWTPGWMPVKRELDRYECRHGLGYT FT RITGERNGVEVSQLAFVPLNYNGEVNQVVITNKSGSEKEIALFSFVEFCLWNAMDDMTN FT FQRNFSTGEVEVEGSAIYHKTEYRERRNHYAFFWVNSPIDGFDTDRESFLGLYNGFDSP FT KNVAAGKPTNSIASGWSPIASHYIKMSLKPGEKRSYIFVLGYVENPPEEKWERKGVINK FT KRAREMQQKFIDDTCVEKAFQELKDYWADLCSKFALESHDEKLNRMVNIWNPYQCMVTF FT NMSRSASYFESGISRGMGFRDSAQDLLGFVHQVPERARQRILDLASTQFEDGSAYHQYQ FT PLTKKGNSDIGSGFNDDPLWLILATAQYIKETGDFGILDEMVPFDCDENKKDTLFEHLK FT RSFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSTQPMNPFTPADKFEGRVAESVFIAGM FT FVLIGPEYVELCKRRGLSEEAAEAEKHIQNMVNAVLTHGYDGEWFLRAYDHFGNKIGSK FT ECSEGQIFIEPQGICVMAGIGVKEGLAQKALDSVMKRLDTKYGIVLHTPAYTEYYLNLG FT EISSYPPGYKENAGIFCHNNPWIAIAETVIGRGDRAFEVYSKIAPAYIEDISDIHRTEP FT YVYSQMIAGRTRWSFGEAKNSWLTGTAAWNFVAITQYILGVRPVYDGLMVDPCIPASWD FT GFTVTREFRGSKYRIRVENPEHICKGVNKVIMDGKEIEGQVLPVSPKESEHEVIVIMG" FT terminator 2746..2763 FT /gene="cepA" XX SQ Sequence 3152 BP; 908 A; 603 C; 804 G; 837 T; 0 other; aaaatgctcg gggagacgtc ggacaatata agcatacata gcctcgaaac agaactgatt 60 gtccgggaca gctgcacggc ggtttccgga gcgggtataa cgaacgacag ttttacagct 120 taataaatcg tcgccggagc tgtcagttgg gataaataaa cgcgcgttca catatttaag 180 ggggtttttg aatgaagttc ggttattttg acgatgtgaa tagggagtat gtaattacca 240 cacctgccac tccttaccct tggattaact accttggctg ccaggatttt ttctcgctga 300 tttcaaacac gtcgggaggc tactgctttt accgcgatgc gagactaagg cgcataacaa 360 ggtaccgtta caataatgtc cccatagaca gcggaggaag gtatttctac atatacgaca 420 gcggagatta ttggacaccg ggctggatgc cggtaaaaag agagcttgac aggtacgagt 480 gccgtcacgg gctgggttac acccgcatta ccggcgaaag aaacggtgtc gaggtaagtc 540 aactggcctt tgtcccatta aactataacg gtgaagtaaa tcaggttgta ataaccaata 600 agagcggcag tgaaaaagag atagcactgt tttccttcgt ggaattctgt ctttggaatg 660 cgatggatga catgacaaat ttccaaagga atttcagcac cggagaggtt gaagtggaag 720 gttcggctat ttaccataaa accgagtaca gggaaagaag aaatcattac gctttcttct 780 gggtcaattc ccccattgac ggattcgaca ctgacaggga atcttttctg ggtctttata 840 acggttttga ttctcccaaa aacgttgcgg cgggaaagcc aaccaattca atagccagcg 900 gctggtcccc gattgcttcg cattatataa aaatgtcgct gaaaccgggc gagaagcgaa 960 gttatatttt cgttctgggg tatgttgaaa atccgcctga ggaaaaatgg gaacgcaaag 1020 gcgtgataaa caagaaaaga gccagagaaa tgcagcaaaa gtttattgat gacacctgtg 1080 tggaaaaagc ttttcaggaa cttaaggatt actgggcgga tttatgctcc aaatttgcgc 1140 tggaaagcca tgatgagaaa ctcaaccgta tggttaatat atggaatccg tatcagtgca 1200 tggttacgtt caatatgtcc agaagcgcgt cgtattttga atcgggcatc agtaggggaa 1260 tgggcttcag ggattccgca caggatcttt tgggctttgt acatcaggtg cccgaacgtg 1320 caaggcagag gatccttgat ttggcttcaa cacagtttga ggacgggagt gcgtatcatc 1380 agtaccagcc cctcaccaaa aaaggcaatt cggatatagg cagtggcttc aatgacgatc 1440 cgctgtggct gattttggct acggcgcagt acataaaaga aacaggggat tttggaattc 1500 tggacgaaat ggtgcccttt gactgcgatg aaaacaaaaa ggatacgctt ttcgaacatc 1560 ttaagcgttc attttatcat gttgttaata accttggccc gcatggatta cctctcatag 1620 gacgggcgga ctggaacgac tgccttaatc tgaactgctt ttcaacacag ccgatgaatc 1680 ctttcacacc tgcggacaaa ttcgagggaa gggtggccga gtcggtattc atagcaggca 1740 tgtttgtcct gatcgggcct gagtatgttg aactgtgcaa gaggcgcggg ctttctgaag 1800 aggcggctga agctgagaaa cacatccaaa acatggttaa tgccgtgctg acccatggtt 1860 atgacggaga atggttctta agggcatatg accattttgg aaacaaaata ggaagcaagg 1920 aatgcagcga gggacaaatc ttcattgaac ctcagggaat ttgcgtgatg gctgggattg 1980 gagtaaaaga aggcctggca caaaaagccc tggattcggt catgaaacgg ctggatacaa 2040 agtacggaat agtgctgcat actccagcgt ataccgagta ttaccttaat ttgggcgaga 2100 tttcgtctta tccgccgggg tacaaggaaa atgcggggat tttctgccac aacaatcctt 2160 ggatcgccat tgccgaaacg gtaatcggac gaggagaccg ggcttttgaa gtctattcaa 2220 aaatcgcgcc tgcatatatt gaagatataa gcgatattca cagaaccgag ccgtatgttt 2280 attcccagat gattgccggc aggacgcggt ggtctttcgg cgaggcaaag aattcatggc 2340 ttacgggtac cgcggcgtgg aactttgttg cgataaccca gtatattctt ggtgtaagac 2400 cggtatatga cgggcttatg gttgatcctt gcattccggc gtcctgggac ggttttacgg 2460 taacaaggga attccgaggt tcaaaataca ggattcgcgt tgaaaatccc gaacatatct 2520 gcaaaggcgt aaataaagta attatggacg gaaaggaaat cgaagggcaa gttcttccgg 2580 tatcgccgaa agaaagtgaa catgaagtaa ttgttataat gggctgacaa tcctgtttta 2640 atttatttaa tcctcctcac tattttataa atccccctgt agcccctctg caaggcaaag 2700 gttgggatat gagtttatat gatatggttc atatcaagaa gtaccggcct tattgccggt 2760 gtttttttat atttaaagaa aggtacaggg ctattatttt ggtgtggtgt cttcaattat 2820 gaggatttta ttgttgcaaa agtgaataat ttttgttttt ggctttgtct gtttttatgc 2880 aacaaattta acaaactgca taattattac acattctagg gtacaagggt gaagagtcct 2940 tttaaatttg gtgatgccgc aagtgatata aaagacataa agatacattc cgattccatt 3000 gaaattgttt ttgatgttaa attgcttgcg gcagaggcaa ccatcccgcc ggaggatata 3060 tcatatgata aggaaaaaga acagaggatc actgtttttg acaattgcaa tgcggtattt 3120 gatacaaatg aagtttcacc ggatattgaa gt 3152 // |
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