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EBI DbfetchID U55819; SV 1; linear; genomic DNA; STD; UNC; 7620 BP. XX AC U55819; XX DT 27-MAY-1996 (Rel. 47, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 4) XX DE Plasmid pRL765 with transposon Tn5 and luciferase (luxA and luxB) genes, DE complete sequence. XX KW . XX OS Plasmid pRL765 OC other sequences; plasmids. OG Plasmid pRL765 XX RN [1] RP 1-7620 RA Cai Y., Wolk C.P.; RT "Plasmid pRL765"; RL Unpublished. XX RN [2] RP 1-7620 RA Wolk C.P.; RT ; RL Submitted (19-APR-1996) to the EMBL/GenBank/DDBJ databases. RL C.P. Wolk, MSU-DOE Plant Research Laboratory, Michigan State University, E. RL Lansing, MI 48824-1312, USA XX DR RFAM; RF00106; RNAI. XX FH Key Location/Qualifiers FH FT source 1..7620 FT /organism="Plasmid pRL765" FT /plasmid="pRL765" FT /mol_type="genomic DNA" FT /note="This plasmid consists of sequences from a variety of FT sources, including transposon Tn5, pBR322, RP-4, ColE1, FT Vibrio fischeri, Amaranthus hybridus, and synthetic DNA" FT /db_xref="taxon:47575" FT gene 359..1423 FT /gene="luxA" FT CDS 359..1423 FT /codon_start=1 FT /transl_table=11 FT /gene="luxA" FT /product="luciferase alpha subunit" FT /note="from Vibrio fischeri" FT /db_xref="GOA:P19907" FT /db_xref="HSSP:1BRL" FT /db_xref="InterPro:IPR002103" FT /db_xref="UniProtKB/Swiss-Prot:P19907" FT /protein_id="AAB00449.1" FT /translation="MKFGNICFSYQPPGETHKLSNGSLCSAWYRLRRVGFDTYWTLEHH FT FTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFNFG FT TVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYPKVY FT SKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDISKID FT HCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQWRDFVL FT QGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDEIIASMR FT RFMTQVAPFLKEPK" FT gene 1462..2442 FT /gene="luxB" FT CDS 1462..2442 FT /codon_start=1 FT /transl_table=11 FT /gene="luxB" FT /product="luciferase beta subunit" FT /note="from Vibrio fischeri" FT /db_xref="GOA:P19908" FT /db_xref="HSSP:1LUC" FT /db_xref="InterPro:IPR002103" FT /db_xref="UniProtKB/Swiss-Prot:P19908" FT /protein_id="AAB00450.1" FT /translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVNE FT HHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGRFI FT LGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSINPH FT CYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYGIDIS FT DVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVGSHDDY FT YESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP" FT gene 3437..4231 FT /gene="npt" FT CDS 3437..4231 FT /codon_start=1 FT /transl_table=11 FT /gene="npt" FT /product="neomycin phosphotransferase" FT /note="from Tn5" FT /db_xref="GOA:Q79C30" FT /db_xref="InterPro:IPR002575" FT /db_xref="UniProtKB/TrEMBL:Q79C30" FT /protein_id="AAB00451.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT CDS complement(5050..6480) FT /codon_start=1 FT /transl_table=11 FT /product="transposase" FT /note="from Tn5" FT /db_xref="GOA:Q57047" FT /db_xref="HSSP:1B7E" FT /db_xref="InterPro:IPR014737" FT /db_xref="UniProtKB/TrEMBL:Q57047" FT /protein_id="AAB00452.1" FT /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS FT IPITSEGSEAMQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR FT HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG FT KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDVESGL FT YLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA FT VLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER FT QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP FT DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS FT KLDGFLAAKDLMAQGIKI" XX SQ Sequence 7620 BP; 1893 A; 1847 C; 1952 G; 1928 T; 0 other; ctgactctta tacacaagta gcgtcctgaa cggaaccttt cccgttttcc aggcgggatc 60 cccgggtacc gagctcattg atagcattga atctagtaat tgtaagctct attcgctaat 120 tggaagttca catgatcttg gggaaaattt ggttgtatta agaaattttt atcaatcagt 180 gacgaaggca gccttagcat tagatgatgg tttattggat ttagagatag acattattga 240 acctcgattt gaggacgtta caagtattac tgttaaggag cgtagattaa aaaatgaaat 300 tgaaaatgaa ttattagaat tggcttaaat aaacagaatc accaaaaagg aatagagtat 360 gaagtttgga aatatttgtt tttcgtatca accaccaggt gaaactcata agctaagtaa 420 tggatcgctt tgttcggctt ggtatcgcct cagaagagta gggtttgata catattggac 480 cttagaacat cattttacag agtttggtct tacgggaaat ttatttgttg ctgcggctaa 540 cctgttagga agaactaaaa cattaaatgt tggcactatg ggggttgtta ttccgacagc 600 acacccagtt cgacagttag aagacgtttt attattagat caaatgtcga aaggtcgttt 660 taattttgga accgttcgag ggctatacca taaagatttt cgagtatttg gtgttgatat 720 ggaagagtct cgagcaatta ctcaaaattt ctaccagatg ataatggaaa gcttacagac 780 aggaaccatt agctctgata gtgattacat tcaatttcct aaggttgatg tatatcccaa 840 agtgtactca aaaaatgtac caacctgtat gactgctgag tccgcaagta cgacagaatg 900 gctagcaata caagggctac caatggttct tagttggatt attggtacta atgaaaaaaa 960 agcacagatg gaactctata atgaaattgc gacagaatat ggtcatgata tatctaaaat 1020 agatcattgt atgacttata tttgttctgt tgatgatgat gcacaaaagg cgcaagatgt 1080 ttgtcgggag tttctgaaaa attggtatga ctcatatgta aatgcgacca atatctttaa 1140 tgatagcaat caaactcgtg gttatgatta tcataaaggt caatggcgtg attttgtttt 1200 acaaggacat acaaacacca atcgacgtgt tgattatagc aatggtatta accctgtagg 1260 cactcctgag cagtgtattg aaatcattca acgtgatatt gatgcaacgg gtattacaaa 1320 cattacatgc ggatttgaag ctaatggaac tgaagatgaa ataattgctt ccatgcgacg 1380 ctttatgaca caagtcgctc ctttcttaaa agaacctaaa taaattactt atttgatact 1440 agagataata aggaacaagt tatgaaattt ggattatttt ttctaaactt tcagaaagat 1500 ggaataacat ctgaagaaac gttggataat atggtaaaga ctgtcacgtt aattgattca 1560 actaaatatc attttaatac tgcctttgtt aatgaacatc acttttcaaa aaatggtatt 1620 gttggagcac ctattaccgc agctggtttt ttattagggt taacaaataa attacatatt 1680 ggttcattaa atcaagtaat taccacccat caccctgtac gtgtagcaga agaagccagt 1740 ttattagatc aaatgtcaga gggacgcttc attcttggtt ttagtgactg cgaaagtgat 1800 ttcgaaatgg aattttttag acgtcatatc tcatcaaggc aacaacaatt tgaagcatgc 1860 tatgaaataa ttaatgacgc attaactaca ggttattgtc atccccaaaa cgacttttat 1920 gattttccaa aggtttcaat taatccacac tgttacagtg agaatggacc taagcaatat 1980 gtatccgcta catcaaaaga agtcgtcatg tgggcagcga aaaaggcact gcctttaaca 2040 tttaagtggg aggataattt agaaaccaaa gaacgctatg caattctata taataaaaca 2100 gcacaacaat atggtattga tatttcggat gttgatcatc aattaactgt aattgcgaac 2160 ttaaatgctg atagaagtac ggctcaagaa gaagtgagag aatacttaaa agactatatc 2220 actgaaactt accctcaaat ggacagagat gaaaaaatta actgcattat tgaagagaat 2280 gcagttgggt ctcatgatga ctattatgaa tcgacaaaat tagcagtgga aaaaacaggg 2340 tctaaaaata ttttattatc ctttgaatca atgtccgata ttaaagatgt aaaagatatt 2400 attgatatgt tgaaccaaaa aatcgaaatg aatttaccat aataaaatta aaggcaattt 2460 ctatattaga ttgccttttt ggggatcctc tagaaatatt ttatctgatt aataagatga 2520 tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc 2580 ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg 2640 cttggaggag cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac 2700 ttcaagacta actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg 2760 tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac 2820 ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag 2880 gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag 2940 gaacaggaga gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt 3000 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag 3060 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca 3120 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc 3180 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga 3240 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag 3300 tgccaacata gtaagccagt atacactccg ctagaggatc tcaatgaata ttggttgaca 3360 cgggcgtata agacatgtta tactgttgaa taacaaggac ggatctgatc aagagacagg 3420 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 3480 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 3540 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 3600 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 3660 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 3720 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 3780 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 3840 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 3900 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 3960 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 4020 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 4080 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 4140 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 4200 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 4260 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 4320 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 4380 atgctggagt tcttcgccca ccccgggagt cagcgcagaa tggtgaacca gagcaaggga 4440 atatgttacg cgtgacgcag tagcggtaaa cggcagacaa aaaaatgtcg cacaatgtgc 4500 gccatttttc acttcacagg tggaattgta cccttggtgg cgtgacggtg gagctgaaat 4560 cgcacctgcc catcgagcgg caggcccgcg tgatcggtgc cacctggctt gaccagcagt 4620 tgatcgacgg tggctcgggc ttgggcgacc tgggctttag cagtgaggcc aagtaggcga 4680 tacagcagcg cgcggacttc ctggccgaac agggactggc cgagcggcgc gggcagcgcg 4740 tgatcctcac cggaatctgc tcggcagcag cgggctcggg aactggcgca ggccgcgaag 4800 gacattgccg ccgataccgg cctggagcat cgccccgtgg ccgacggcca gcgcgttgcc 4860 ggcgtctacc ggcgccccgt catgctcgcc agcgggcgaa atgggatgct tgatgacgcc 4920 aaggggtcca gcctcgtgcg gtggaagccc atcgaacagc ggcttgggga gcagctcgcc 4980 gcgacggtgc gcggtggcgg cgtgtcttgg gagattggac gacagcgtgg gccggcccct 5040 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc 5100 cagtttactt tgcagggctt cccaaccttc ccagagggcg ccccagctgg caattccggt 5160 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct 5220 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc 5280 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag 5340 cccttgcgcc ctgagtgctt gcggcagcgt gaagctttct ctgagctgta acagcctgac 5400 cgcaacaaac gagaggatcg agaccatccg ctccagatta tccggctcct ccatgcgttg 5460 cctctcggct cctgctccgg ttttccatgc cttatggaac tcctcgatcc gccagcgatg 5520 ggtataaatg tcgatgacgc gcaaggcttg ggctagcgac tcgaccggtt cgccggtcag 5580 caacaaccat ttcaacgggg tctcaccctt gggcgggtta atctcctcgg ccagcaccgc 5640 gttgagcgtg atattcccct gttttagcgt gatgcgccca ctgcgcaggc tcaagctcgc 5700 cttgcgggct ggtcgatttt tacgtttacc gcgtttatcc accacgccct tttgcggaat 5760 gctgatctga tagccaccca actccggttg gttcttcaga tggtcgatca gatacaaccc 5820 agactctacg tccttgcgtg ggtgcttgga gcgcaccacg aagcgctcgt tatgcgccag 5880 cctgtcctgc agataagcat gaatatcggc ttcgcggtca cagaccgcaa tcacgttgct 5940 catcatgctg cccatgcgta accggctagt tgcggccgct gccagccatt tgccactctc 6000 cttttcatcc gcatcggcag ggtcatccgg gcgcatccac cactcctgat gcagtaatcc 6060 tacggtgcgg aatgtggtgg cctcgagcaa gagaacggag tgaacccacc atccgcggga 6120 tttatcctga atagagccca gcttgccaag ctcttcggcg acctggtggc gataactcaa 6180 agaggtggtg tcctcaatgg ccagcagttc gggaaactcc tgagccaact tgactgtttg 6240 catggcgcca gcctttctga tcgcctcggc agaaacgttg ggattgcggt aaaatcggta 6300 agcgccttcc tgcatggctt cactaccctc tgatgtgatg gggattgatt taccagaata 6360 ttttgccaat tgggcggcga cgttaaccaa gcgggcagta cggcgtggat cacccagcgc 6420 cgccgaagag aacacagatt tagcccagtc ggccgcacga tgaagagcag aagttatcat 6480 gaacgttacc atgttaggag gtcacatgga atatcagatt ctggaaaacg ggaaaggttc 6540 cgttcaggac gctacttgtg tataagagtc aggtgagtta tgttgttgct ttacttttta 6600 gcttgcttgc tggaactaca ttaggtattt ggggtattgc tattgttaca ggaattcatc 6660 gagggatcgt cttgccttgc tcgtcggtga tgtacttaca gctcgaagtg cctcttcttg 6720 atggaggcca tggaacgtgc ttccgaatca cgcgcacccc gatccgttac gtgaaaaaag 6780 tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt 6840 ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg 6900 gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg 6960 ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac 7020 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct 7080 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt 7140 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag 7200 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc 7260 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg 7320 aaagtctacg cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac 7380 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaagatc gtcgacctgc 7440 aggcatgcaa gctttcgcga gctcgagatc tagaatcgtc cattccgaca gcatcgccag 7500 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct 7560 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc 7620 // |
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