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EBI DbfetchID U53868; SV 1; linear; genomic DNA; STD; PRO; 6010 BP. XX AC U53868; XX DT 08-APR-1998 (Rel. 55, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Clostridium acetobutylicum mannitol-specific phosphotransferase system DE (PTS) system, mtlA, mtlR, mtlF, and mtlD genes, complete cds. XX KW . XX OS Clostridium acetobutylicum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-6010 RX PUBMED; 11160802. RA Behrens S., Mitchell W.J., Bahl H.; RT "Molecular analysis of the mannitol operon of Clostridium acetobutylicum RT encoding a phosphotransferase system and a putative PTS-modulated RT regulator"; RL Microbiology (Reading, Engl.) 147(Pt 1):75-86(2001). XX RN [2] RP 1-6010 RA Behrens S.; RT ; RL Submitted (08-APR-1996) to the EMBL/GenBank/DDBJ databases. RL S. Behrens, Institut fuer Mikrobiologie, Georg-August-Universitaet RL Goettingen, Grisebachstrasse 8, Goettingen D-37077, Germany XX DR CABRI; DSM 792. XX FH Key Location/Qualifiers FH FT source 1..6010 FT /organism="Clostridium acetobutylicum" FT /strain="DSM 792" FT /mol_type="genomic DNA" FT /db_xref="taxon:1488" FT CDS <1..251 FT /codon_start=3 FT /transl_table=11 FT /gene="orfP" FT /note="similar to the CBBY/CBBZ/YHFE/YIEH family" FT /db_xref="GOA:O65988" FT /db_xref="InterPro:IPR005834" FT /db_xref="UniProtKB/TrEMBL:O65988" FT /protein_id="AAC12847.1" FT /translation="VEKGKPHPEIFLRAASMFDDNILNFTVIEDTNNGVRAAKSAKMKC FT VGFSNPNSGTQNISSADIIVDNFGDESISRIINLVLK" FT terminator 264..303 FT /note="putative" FT misc_signal 401..414 FT /note="catabolite responsive element CRE1 (by similarity)" FT -35_signal 434..439 FT /note="experimentally by primer extension and by FT similarity" FT misc_signal 435..448 FT /note="catabolite responsive element CRE2 (by similarity)" FT -10_signal 457..462 FT /note="experimentally by primer extension and by FT similarity" FT RBS 500..505 FT /gene="mtlA" FT misc_feature 512..1538 FT /gene="mtlA" FT /note="encodes EIIC domain (by similarity)" FT CDS 512..1957 FT /codon_start=1 FT /transl_table=11 FT /gene="mtlA" FT /product="MtlA" FT /note="mannitol-specific PTS EIIBC component" FT /db_xref="GOA:O65989" FT /db_xref="InterPro:IPR004718" FT /db_xref="UniProtKB/Swiss-Prot:O65989" FT /protein_id="AAC12848.1" FT /translation="METYKDSTQVSKSSLKKKIQGFGGFLSGMVMPNIGAFIAWGLITA FT LFIKTGWLPNDNLSKLVDPMIHYMLPMLIGYQGGKLVYDTRGGVVGAIATMGMIVGASI FT PMFLGGMIIGPLGGYVIKKFDKAIENKIPTGFEMLVNNFSAGILGAALAIISYVAVGPV FT VAGASTGLGSIALAITNQGLLPLIAVVVEPAKILFLNNAINHGVFSPLGIEQVQHLGKS FT VFFLLEADPGPGLGILLAYSLYGKGSAKNSAPGAVIIHFLGGIHEIYFPYVLMKPFLLL FT AVIAGGICADLTFVLLKAGLVAAASPGSIIAILAMSPKGGQLPVLAGVAVGAIVSFVVA FT SIILKGSKEKSKDNFEEAQNKMKEMKKESKNQTTANSENVKNNDELVSSDIKLIVFACD FT AGMGSSAMGESILKEELKNANIDGIKVQHYSVDSIPKERSVVFVQENLSERARKSAPDA FT NIVTIKNFLDRSTYEGFMKKIKK" FT misc_feature 1539..1954 FT /gene="mtlA" FT /note="encodes EIIB domain (by similarity)" FT misc_feature 1703..1705 FT /gene="mtlA" FT /note="encodes Cys residue modified by phosphorylation (by FT similarity)" FT RBS 1968..1973 FT /gene="mtlR" FT CDS 1979..4033 FT /codon_start=1 FT /transl_table=11 FT /gene="mtlR" FT /product="MtlR" FT /note="putative PTS multidomain regulator" FT /db_xref="GOA:O65990" FT /db_xref="InterPro:IPR011608" FT /db_xref="UniProtKB/TrEMBL:O65990" FT /protein_id="AAC12849.1" FT /translation="MNSLTPRQQYILSELIKKKVSNVDVLHKELAVSIRTILREVASIN FT KVLKKDKITIYDYENMNLVISGDRDSIKNLENSINYIPILWLCNKDQRQIAITCKLLSE FT EEYLKASYFSYKFNVVMGSISQDLDNIQTLLVNKNLHLIRKRTYGIMIDGSEWNKRNAF FT VELFFRFKPFEYLLSFLYGHKTDETVEAFFKDIFDAKTIDIVKEIFKKLHFGSTNVNDI FT KYFSLFLLTLLAIKKTESKKNINLPNKVKQDMKSSIIYNTLRSLQEKLMENNIILPEDE FT LSYLCLHLNDYKYYNDAEENNIEADINYTEISNELVTGVSKKIGINITKDKKLVEDLSN FT HLKQTFHMLSVGLDIINPLLNEIKEHYSQLFAIINNECKLIFSRYNIKILEEEIGYITM FT HIDVAIQRQQIFSKKINLLIVCPNGMSSGNILGNKVKSLFSDIESITISSIHDAYEKMV FT NNEYDLILSTVPISLQISNVIVVSPFMTNEDIEEVRNFIFKIKMEKQNIAIDFSDRLKI FT DATNMEYETISSILKSFDVREVKVNTFDELIDYIVTDIYDSNLSKSKEAIKKLILKREE FT KGNVVVPKSGIALLHTRSNELMNPVIRVYRNNKYFPMSSEGFSTEDINTFLVLLARNDE FT SNYVLQFLGKVSISLMERKGFVQMLRYSNSQDIRNCLIDIANKEEVIYE" FT misc_feature 2936..3187 FT /gene="mtlR" FT /note="encodes domain similar to antiterminators of the FT BglG family" FT misc_feature 2999..3011 FT /gene="mtlR" FT /note="encodes His residue modified by phosphorylation (by FT similarity)" FT misc_feature 3629..3983 FT /gene="mtlR" FT /note="encodes domain similar to EIIANtr-proteins" FT misc_feature 3749..3752 FT /gene="mtlR" FT /note="encodes His residue modified by phosphorylation (by FT similarity)" FT RBS 4011..4016 FT /gene="mtlF" FT CDS 4026..4445 FT /codon_start=1 FT /transl_table=11 FT /gene="mtlF" FT /product="MtlF" FT /note="mannitol-specific PTS EIIA component" FT /db_xref="GOA:O65991" FT /db_xref="HSSP:1A3A" FT /db_xref="InterPro:IPR002178" FT /db_xref="UniProtKB/TrEMBL:O65991" FT /protein_id="AAC12850.1" FT /translation="MNRQILSGDNILLELKSESKDAAIERAGKLLVSRGYVKENYINGM FT KAREEEVTTYMGNGVAIPHGMNEYKKDIMDSGIVIAQYPEGVDFGEGNIAYIVIGIAGR FT GEEHMQILSQIALRFNMKKMLKDLEMLKIKRKLLR" FT misc_feature 4215..4217 FT /gene="mtlF" FT /note="encodes His residue modified by phosphorylation (by FT similarity)" FT RBS 4450..4455 FT /gene="mtlD" FT CDS 4467..5621 FT /codon_start=1 FT /transl_table=11 FT /gene="mtlD" FT /product="MtlD" FT /EC_number="1.1.1.17" FT /note="mannitol-1-phosphate dehydrogenase" FT /db_xref="GOA:O65992" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:O65992" FT /protein_id="AAC12851.1" FT /translation="MKALHFGAGNIGRGFIGYLLYKSNYETTFVDIFDKVVDDINKYKR FT YTVITLSTSKNKEKVENVRAVNLKDSVALEKEVLEADLITTSLGLNNLKSTGELLRGFL FT KKRSEINDKPLDIIACENALFASDVLKKAILDGADEELKKYLEKSVGFPNCTVDRIVPN FT VDIEKELPIDVAVEDFYEWDIEKNKVKINNKIIGAEYVEKLDPYLERKLFLLNGAHATI FT AYLGYLKGYKYIHEAIKDKEINKIIVGFHSEAVQALSEKHKIDIQILKEYSNKLLKRFE FT NEYLKDDVSRVGRDPMRKLSSNDRLITPLKLCCDLKIDFTNILFGVASGYLFNYKEDEK FT AQGIQNIITKEGIKKAISNVSQIKEGDYLNNMIAYKYEELKKQN" FT misc_feature 4473..4517 FT /gene="mtlD" FT /note="encodes NAD binding motif (by similarity)" XX SQ Sequence 6010 BP; 2368 A; 636 C; 1110 G; 1896 T; 0 other; ctgtagagaa gggaaaacca catccggaaa tattcttaag agcagcatct atgtttgatg 60 acaatatctt aaattttact gtaattgaag atacaaataa tggtgttaga gctgcaaaga 120 gtgcaaaaat gaaatgtgta gggtttagta atccaaattc gggaactcaa aatattagca 180 gtgcggatat tattgtagat aatttcggtg atgaaagcat aagtagaatt ataaatttag 240 ttttaaaata gtgatttttt attaacagag gttaggattt taattaaaat cctaacctct 300 gttttgtttt caagaactta tttttttctt ttttaagttg taataagatg ttgcaatgtg 360 gcctaaatcc tagtaatttt aataacttta aaagtgtcat tgaaaacgta taccaaaaat 420 gaacttgtcc acgttgaaaa cgatcgcatt ttgttttatc atgttaattg taaggaataa 480 taataatata aaataatatg gaggatacga tatggaaact tataaagatt ctacacaagt 540 atcaaaatca tcattaaaga aaaagattca aggttttggt ggttttttaa gtggcatggt 600 aatgcccaat ataggagcct ttatagcttg gggtttaata acggcattat ttattaaaac 660 agggtggtta cccaacgata atctttccaa acttgtagat ccaatgatac attatatgct 720 tcctatgctt ataggatatc aaggtggtaa gcttgtatat gatacaagag gtggtgtagt 780 aggagcaata gctaccatgg gtatgatagt aggagcttca ataccaatgt tccttggagg 840 aatgataata ggtcctttag gcggatatgt tataaagaag ttcgataagg ctattgaaaa 900 taaaattcca acaggctttg agatgttagt taataacttt tcagctggaa ttttaggtgc 960 ggctcttgca ataatctcat atgttgcagt tggtcctgta gttgcaggag catcaacagg 1020 attaggttcg atagcattag ctattacaaa tcaaggatta cttccactaa tagcggtagt 1080 agtagagcca gctaagatat tatttttaaa caatgctatt aaccacggtg tattctcacc 1140 acttggaatt gaacaagtac aacatcttgg taaatcagta ttcttcttac ttgaagcaga 1200 tccaggtccg ggactaggaa ttcttttagc ttactcttta tatggcaagg gttcagcaaa 1260 aaattcagct ccaggggcag ttataataca ttttcttggc ggaatacatg aaatttactt 1320 cccttatgtt ttaatgaaac cgtttttact tttagcagta atagctggtg gaatttgtgc 1380 agatttaacg tttgttttat taaaagcagg tttagttgca gcagcttcac caggaagtat 1440 catagctata ctggcaatga gtcctaaagg tgggcaactt ccagtattag caggagttgc 1500 ggtaggagca atagtatctt tcgtagtagc atcaattatc ctaaaaggaa gtaaagaaaa 1560 atcaaaagat aattttgaag aagctcaaaa caagatgaaa gaaatgaaaa aagaaagcaa 1620 aaatcaaaca actgctaata gtgaaaatgt taaaaataat gatgaattag taagttctga 1680 tattaaatta atagtttttg catgtgatgc aggtatggga tcatcagcaa tgggagaatc 1740 tatattaaag gaagaactaa aaaatgcaaa tatcgatgga ataaaagttc aacattattc 1800 agtagatagt attccaaaag aacggagtgt agtatttgtg caagaaaatt taagcgaaag 1860 agcaagaaaa agtgctccag atgctaatat agtaacaata aaaaatttct tagatcgttc 1920 aacttatgaa gggtttatga aaaaaattaa aaaataaata gtaactaggt gagtgagtat 1980 gaattcttta actcctagac agcagtatat actaagtgag ttaataaaaa aaaaggtgtc 2040 taatgtagat gttcttcata aagagcttgc agtaagtata agaactattt taagagaggt 2100 tgcatctata aataaggttt taaaaaaaga taaaataact atctatgatt atgaaaatat 2160 gaatttggtt atatctgggg atagagatag tattaaaaat ttggaaaatt caattaatta 2220 tatccccatt ttatggctat gcaataaaga tcaaaggcag atagcaatta catgtaaatt 2280 gttatccgaa gaagaatatt taaaagcatc atattttagc tacaaattta atgtagtaat 2340 gggaagcata agtcaggatt tggataatat tcagacatta cttgtaaaca agaacctaca 2400 tcttataaga aaaagaacct atggaataat gatagatggt tccgaatgga ataaaagaaa 2460 tgcttttgtt gagttatttt tcagatttaa accttttgaa tatttgttat cattccttta 2520 tggacataag acagatgaaa cagtggaagc tttttttaaa gatatatttg atgcaaaaac 2580 aatagatatt gttaaggaaa ttttcaaaaa acttcatttt ggtagtacta atgttaatga 2640 tattaagtat tttagcttat ttttactaac acttcttgct ataaaaaaga cagaaagcaa 2700 aaaaaatata aatctaccga ataaagtaaa gcaggatatg aaaagtagca ttatatataa 2760 cacactaaga tcactgcaag aaaaattgat ggaaaataat ataatattac ctgaagatga 2820 gttaagttat ttatgtttac atttaaatga ttacaaatac tataatgatg ccgaagaaaa 2880 taacattgag gcagacatta attatacaga gataagcaat gaattggtta caggggtatc 2940 aaaaaaaata ggaataaata ttactaaaga taaaaaattg gtagaggatt tatctaatca 3000 tttaaaacaa accttccata tgcttagtgt aggacttgat attataaatc ctctattaaa 3060 tgaaattaaa gaacactatt ctcaattgtt tgccataata aacaatgagt gtaaacttat 3120 tttttctaga tataatataa aaatattgga agaagaaatt ggatatataa caatgcatat 3180 agatgtagcc attcaaagac aacagatatt cagtaaaaag ataaatctgc ttattgtatg 3240 tcctaatggt atgagtagtg gaaacatatt aggtaataag gttaaatcac ttttttcaga 3300 tatagaaagt ataactatta gttctataca tgatgcttat gaaaagatgg ttaataatga 3360 gtatgattta attttatcaa cagtgccaat aagtttacaa ataagcaatg taatagtcgt 3420 ttcacctttt atgacaaatg aggacattga agaagtaaga aattttatct ttaaaataaa 3480 aatggagaag cagaatatag ctatagattt ttcagataga cttaaaatcg atgctacaaa 3540 tatggagtat gaaactatta gttccatatt gaaaagtttt gatgtaaggg aggtaaaagt 3600 taatactttt gatgaattga tagattatat tgtaacagac atttatgata gtaatttaag 3660 caaaagcaaa gaagcaatta aaaagctaat actaaaaaga gaggaaaaag gtaatgttgt 3720 agttcctaaa agtggaatag cattacttca tactagaagt aatgaactta tgaatccagt 3780 tataagagtg tatcgtaata ataaatattt tccgatgtca agtgaaggtt tttcaacaga 3840 ggacataaat acttttttag tgcttttagc tagaaatgat gagagtaact atgtgttaca 3900 gttcttaggg aaagtaagta tttcattaat ggaaagaaaa ggatttgttc aaatgttgag 3960 atatagtaat tcgcaggata ttaggaactg cttaatagat attgccaata aggaggaagt 4020 tatttatgaa tagacagatt ttatcaggag ataacatttt attagagttg aaatcagaat 4080 ctaaggatgc tgcaatagaa agagctggaa aacttttagt ttctagaggt tatgttaagg 4140 aaaactatat aaatggaatg aaagccagag aagaagaagt tacaacatat atggggaatg 4200 gtgtcgctat acctcatggt atgaatgaat ataaaaaaga tattatggat tcgggtatag 4260 ttatagctca atatcctgaa ggcgttgact ttggtgaagg aaatatagct tatatagtta 4320 taggaattgc aggtagagga gaagaacata tgcaaatact atctcaaata gccttacggt 4380 tcaatatgaa gaaaatgttg aaagacttag aaatgctaaa aataaagagg aaattattaa 4440 gataatagag gaaggtgatg actagaatga aagcacttca ttttggagca ggaaacatag 4500 gaagaggttt tataggttat ttactgtata agtctaatta cgaaactacc ttcgttgata 4560 tatttgataa agtagtggat gatatcaata aatataaaag atatacagta ataactttga 4620 gtacatctaa gaataaggag aaagttgaga atgtacgagc agtcaatttg aaagatagtg 4680 ttgctcttga aaaagaagtt ttagaagctg atttaataac tacatcatta ggattaaata 4740 atttaaaaag tacaggagag cttttaagag gattccttaa aaagagatca gaaatcaatg 4800 acaagccact tgatataata gcatgtgaaa atgcactatt tgcaagtgat gtactaaaaa 4860 aagctatact tgatggtgca gatgaagagt tgaaaaaata cttggaaaaa agtgttggat 4920 ttccaaattg tacagttgat aggattgtgc caaatgtgga catagaaaaa gaattgccta 4980 tagatgtagc agtagaagat ttttatgaat gggatataga aaaaaataag gttaaaatta 5040 ataacaagat aattggtgca gaatatgttg aaaaattaga cccttatctt gaaagaaagt 5100 tattcctttt aaatggtgca catgctacaa ttgcatattt aggatatctt aaaggatata 5160 aatatataca tgaagctata aaagataagg aaattaataa gattatagtt ggttttcata 5220 gcgaagcggt acaagctctt agtgaaaagc ataaaataga tatacaaatc ttaaaggaat 5280 attctaataa acttctaaag agatttgaaa atgaatactt aaaggacgat gtaagtcgtg 5340 taggtagaga tccgatgaga aaattatcgt ctaatgatag attgataact cctttaaaat 5400 tatgttgcga tttgaaaata gattttacta atatattatt tggagttgct tctggatatc 5460 tatttaatta taaagaggat gaaaaagctc aaggaattca aaacataata actaaagagg 5520 gaattaaaaa agctatttca aatgtatctc aaataaaaga aggagattat ttgaataata 5580 tgatagcgta caagtatgag gagctaaaaa agcaaaatta aaatttggaa tattcataat 5640 cttcaaaata ttattgctct aattgttttt taagaattga attcgattta ttttaggggg 5700 ggaaatattt attaaaataa tttatataat ttgctatttg gtataattat gtaaatatat 5760 taaaaatatt ttcctcattt tataaactta atacatatac ttgcataata tattttatgt 5820 ataaaagtaa aaaaaatcaa taaatgtgtt tttataatca ttgacaggct aataaaagta 5880 atagtataat gacaatgtct tatagacaag taaattgagg aggaaagtag tgttaaaatt 5940 atagttaaag cgcccgaact tgagtgatat agttcattta agttgacgag gatggggaga 6000 atcgaatctt 6010 // |
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