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EBI DbfetchID U47861; SV 1; linear; genomic DNA; STD; PRO; 4106 BP. XX AC U47861; XX DT 08-SEP-1996 (Rel. 49, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus subtilis gbsAB operon, glycine betaine aldehyde dehydrogenase DE GbsA, alcohol dehydrogenase GbsB genes, complete cds. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4106 RX PUBMED; 8752328. RA Boch J., Kempf B., Schmid R., Bremer E.; RT "Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: RT characterization of the gbsAB genes"; RL J. Bacteriol. 178(17):5121-5129(1996). XX RN [2] RP 1-4106 RA Kempf B.; RT ; RL Submitted (01-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Bettina Kempf, Biology/Microbiology, Philipps-University Marburg, RL Karl-von-Frisch Strasse, Marburg, 35032, Germany XX FH Key Location/Qualifiers FH FT source 1..4106 FT /organism="Bacillus subtilis" FT /strain="JH642" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS complement(<1..324) FT /codon_start=1 FT /transl_table=11 FT /note="ORF-1 upstream of gbsAB operon" FT /db_xref="GOA:P71014" FT /db_xref="UniProtKB/Swiss-Prot:P71014" FT /protein_id="AAC44367.1" FT /translation="MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQ FT STASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALR FT TTQI" FT CDS complement(522..1064) FT /codon_start=1 FT /transl_table=11 FT /note="ORF-2 upstream of gbsAB operon" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:P71015" FT /protein_id="AAC44366.1" FT /translation="MDENPEFAAIEQARDLVIDSIAETMDLYGITRSVGILYGTMYMRD FT EMTLDEMREELQMSKPSMSTGVKKLQDLNVVKKTFHRGIRKHTFVAEKDFFKFFTNFFP FT PKWEREVQVNVTAIEEAQADLQKVLCKEDLDEDIKNEALQLYDQLESSKAYYDWLKRLA FT ESVQTGEIFKFIPVETK" FT RBS 1251..1257 FT /gene="GbsA" FT CDS 1263..2735 FT /codon_start=1 FT /transl_table=11 FT /product="GbsA" FT /note="glycine betaine aldehyde dehydrogenase" FT /db_xref="GOA:P71016" FT /db_xref="InterPro:IPR011264" FT /db_xref="InterPro:IPR015590" FT /db_xref="InterPro:IPR016160" FT /db_xref="InterPro:IPR016161" FT /db_xref="InterPro:IPR016162" FT /db_xref="UniProtKB/Swiss-Prot:P71016" FT /protein_id="AAC44364.1" FT /translation="MSQTLFIDGEWISAEKEQIRSIINPFNQEEIATVSEGGREDAIKA FT IAAARRAFDKGEWSSLSGLERGKIVLKIAELIRRDLEELAELESLDTGKTLEESKADMD FT DIANVFQYYAGLADKDGGEIISSPIPDSESKIIREPIGVCGQITPWNYPLLQASWKIAP FT ALAAGNTIVMKPSEITPLTTIKVFKLMEEAGVPKGVANLVLGPGATVGDELAVNKDVDL FT ISFTGGIETGKKIMRAASGNVKKIALELGGKNPNIVFKDADLEVAVDQALNAVFFHAGQ FT VCSAGSRLLVEDAIHDQFLAELVKRAKRIKLGNGFHAETESGPLISAEHRAKVEKYVEI FT GIEEGAKLETGGKRPEDPELQNGFFYEPTIFSNCNSDMRIVQEEVFGPVLTVETFSSEE FT EVIELANDTIYGLAGAVWSKDIEKCERVAARLRMGTVWINDFHPYFAQAPWGGYKQSGF FT GRELGKIGLEEYTEVKHVYRNTKPAAVNWFNS" FT RBS 2764..2771 FT /gene="GbsB" FT CDS 2779..3960 FT /codon_start=1 FT /transl_table=11 FT /product="GbsB" FT /note="alcohol dehydrogenase; glycine betaine synthesis" FT /db_xref="GOA:P71017" FT /db_xref="InterPro:IPR001670" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:P71017" FT /protein_id="AAC44365.1" FT /translation="MQKFHTFEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYK FT AGVADPVIESLKEAGIEVVLFNKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDT FT AKAIGVEATHEGSVLDYEAADGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREF FT KFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALM FT AIEYAAHYIKRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAH FT GQCVAAMMGPVMEYNWKGYPEKFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEV FT PTLEEQGVSPDMIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPKTV" FT terminator 3963..3993 FT /note="terminator of gbsAB operon" XX SQ Sequence 4106 BP; 1237 A; 845 C; 976 G; 1048 T; 0 other; gatctgtgtt gtacgcaagg catttccgtt caatgtgtct ttcgtgtttg cagtaaatcc 60 gcttggcaat gtaaactcca taacgccaag gctgagaagc gtgtttggac ggtaagtcaa 120 ttcgatatct gagaaagacc attccgtttt gctggcgcca gtgattgttg cgaacaatga 180 agctgtagac tgtgttctca tagtggattc agtatgagct ttagttgatg tagattcagc 240 cgcgaaagaa gctgtaggtg cagaaacgag taacccgaga cttaatgcac taattgccaa 300 agaagataat aatttgcgtt tcataacaaa attcccccta aaaaatgata aattaaacag 360 gcctgcccgc ttttcacctc ctctgaaagg ccaaatacaa attttaccta cataaaatta 420 ccatcaaata ttttggaagt aaatggggtg tttttcccgt atttttcaaa tttgtagtag 480 ttacatcaaa aaaacgccta gtttcccagg cgttttctgc tttactttgt ttcgaccggt 540 ataaatttaa aaatttctcc ggtctgcacc gattcagcaa gccgtttcag ccagtcatag 600 taagctttag agctttccag ctgatcatat aactgcagtg cttcattttt gatatcctca 660 tcgagatctt ccttgcataa aaccttttgt aaatcagctt gagcttcctc gatcgctgtt 720 acattcactt gaacctcccg ctcccattta ggtggaaaga aatttgtgaa aaatttaaag 780 aaatccttct cagccacaaa tgtatgcttg cggatgcccc ggtgaaatgt ttttttcact 840 acatttaagt cttgaagctt tttgacgcca gtgctcatgc ttggtttgct catttgcaac 900 tcctcacgca tttcgtcaag cgtcatctca tccctcatat acatcgtccc atataaaatc 960 ccgacactgc gggtaattcc gtaaagatcc atcgtttcag caatggagtc aataacaaga 1020 tcccgagcct gttctatagc tgcaaattct ggattttcat ccatactgac cctcttttcc 1080 tttagaaaat taaattcgtt aagttatttc tttatgttct ttatcttaac gaaacatgag 1140 gaaaagtcaa aaagaaaaat caaaatggga ctttgacagt ttaaaaacca tatgttagat 1200 taaggttgtt aaaaacatta aatttttatt taacaaactt tatttacgtc aaggaggctt 1260 atatgagtca aacattattc attgacggag aatggatcag tgccgaaaaa gaacagatcc 1320 gcagtattat caacccattt aatcaagaag aaattgcaac tgtaagcgag ggagggcgag 1380 aggacgccat caaagcaatc gcagccgcac gtagagcatt tgacaaagga gaatggtcat 1440 cgctatccgg gcttgaacgc ggaaaaattg ttctgaaaat tgcggaatta atcagacgcg 1500 atcttgagga gctggctgag cttgaatctc ttgatacagg aaaaacactc gaagaaagca 1560 aggccgatat ggacgatatc gccaatgttt ttcaatatta cgccggcttg gcagacaaag 1620 atggcggaga gatcatttca tctccgattc ctgattcaga aagcaaaatt attagggagc 1680 caatcggggt ttgcggccag atcactccat ggaattatcc gctccttcaa gcgagctgga 1740 aaatcgcccc tgcactggcc gcaggaaaca caatcgtcat gaagccgagt gagattacgc 1800 cgctgacgac aatcaaagtc tttaagctga tggaagaagc cggtgttcca aaaggtgtcg 1860 caaatcttgt tcttggaccg ggagccacag tgggcgacga gcttgccgta aacaaagacg 1920 tcgatttgat ttcatttacg ggcggaattg aaacaggcaa aaaaatcatg cgggcggcaa 1980 gcggaaacgt caaaaaaatc gcccttgaac ttggcgggaa aaacccaaat attgttttca 2040 aggacgcgga tttagaagtt gcggttgatc aggccttaaa cgctgtattt ttccacgccg 2100 gccaagtatg ctctgcaggt tcccgcttgc ttgttgagga tgccattcat gatcaatttt 2160 tggcggagct ggtcaaacgg gcaaaacgca taaaactcgg aaacggtttt catgctgaga 2220 cagaaagcgg tccgcttatt tcggcggagc acagggcaaa ggtagaaaaa tatgtagaga 2280 tcggaataga ggaaggcgcg aagctggaga caggaggcaa acgcccggaa gatcctgagc 2340 ttcaaaacgg ctttttctat gaacctacta ttttctcaaa ctgtaattct gacatgagaa 2400 tcgttcagga agaggttttc ggtcctgtat tgacagtcga aaccttcagc tctgaagaag 2460 aggtaatcga gcttgcgaat gataccatct atggcttggc tggagcggta tggtcaaaag 2520 acattgaaaa gtgcgaacgg gtagcagccc gcttgagaat gggaaccgtt tggatcaacg 2580 attttcatcc gtactttgca caagcgccat ggggcggata taagcaatcc gggttcggac 2640 gcgagcttgg aaaaataggc cttgaagaat acacagaagt caaacatgta taccgcaata 2700 caaaaccggc agcggttaac tggtttaatt cataagaggg ggagattgat gatgacatta 2760 aatatgaagg tagaaagcat gcagaaattc cacacatttg aaattccgac tgtgattaag 2820 catggaatcg gagccatcaa gcatacgggt gaggaagtag ctgcactggg cgtttcaaaa 2880 gcgctccttg tcacagaccc tgggatttat aaagccggtg ttgctgatcc cgtaatcgaa 2940 tcacttaaag aagcaggcat tgaagtggta ctctttaata aagttgagcc aaacccgcct 3000 gtccgtctgg tgaatgaagg atctgagctt tacaaaaaag agaactgtaa tggtttggtg 3060 gcagtcggag gcggaagctc catggataca gcaaaagcaa tcggagtaga agcgactcac 3120 gaaggaagcg tgcttgatta tgaagcggca gatgggaaaa aaccgctgga aaaccgcatt 3180 cctccgctga cgacaattcc cactacagct ggaaccggat cagaagtaac ccaatgggcg 3240 gttatcacag atgaagaaag agaattcaaa ttcaacacgg gcggtccgct gattgcagcg 3300 cacttgacaa tcattgatcc tgagcttcat gtttcaatgc ctccgcatgt aacagctatg 3360 acgggaattg atgcgctcgc ccatgccatt gagtgctata caatgaaatt tgcacagcca 3420 atcacggatg cggttgcctt aatggcgatt gagtatgccg ctcattacat caagagggca 3480 tttgctgatg gagaggatct ggaagcaagg tacggaatgg cgcaggctgc gatgcttgcc 3540 ggtctttctt acggaagtga atcagcgggc gctgctcatg cgatgagcca aacgcttggc 3600 ggtattatcc cagtcgctca cggccaatgt gtagcggcga tgatgggccc tgtcatggaa 3660 tacaactgga agggctatcc tgagaagttt gcgcgtattg cgaaagcgtt cggtattgat 3720 acaagcaaga tgacaacaga agaagctgcg aaagcatctg tcaattggat gtatgatctg 3780 gtcgaagatt tagaagtgcc gactttagaa gagcagggag tatcacctga tatgatcgaa 3840 cgcttgtcta aagaagcaat gaaagatccg caaaccttcg gaaaccctag agatttaaat 3900 gaaaaagcat acaactggat ctacaaacgc tgctttaacc ttacgcctaa aacggtataa 3960 tcaaaatccc gctcctttac agagcgggat tttttgggat aatgaagcat aggtggaaat 4020 tttagataag aaggaaggtg ccagctgatg tggaagcgta tgactgcaaa agcggaaggc 4080 ttatatatcg cagatacaaa aagctt 4106 // |
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