EBI Dbfetch

ID   U41388; SV 1; linear; genomic DNA; STD; PRO; 1789 BP.
XX
AC   U41388;
XX
DT   20-JAN-1996 (Rel. 46, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 5)
XX
DE   Mycobacterium bovis putative ketoacyl ACP reductase and enoyl ACP reductase
DE   (inhA) gene, complete cds.
XX
KW   .
XX
OS   Mycobacterium bovis
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
XX
RN   [1]
RP   1-1789
RX   DOI; 10.1126/science.8284673
RX   PUBMED; 8284673.
RA   Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T.,
RA   Collins D., de Lisle G., Jacobs W.R.Jr.;
RT   "inhA, a gene encoding a target for isoniazid and ethionamide in
RT   Mycobacterium tuberculosis";
RL   Science 263(5144):227-230(1994).
XX
RN   [2]
RP   1-1789
RX   DOI; 10.1111/j.1365-2958.1995.tb02276.x
RX   PUBMED; 7623658.
RA   Wilson T.M., de Lisle G.W., Collins D.M.;
RT   "Effect of inhA and katG on isoniazid resistance and virulence of
RT   Mycobacterium bovis";
RL   Mol. Microbiol. 15(6):1009-1015(1995).
XX
RN   [3]
RP   1-1789
RA   Collins D.M.;
RT   ;
RL   Submitted (27-NOV-1995) to the EMBL/GenBank/DDBJ databases.
RL   D.M. Collins, Animal Health Division, Agresearch, Ward Street, Upper Hutt,
RL   P.O. Box 40063, New Zealand
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1789
FT                   /organism="Mycobacterium bovis"
FT                   /strain="WAg201"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1765"
FT   gene            1..162
FT                   /gene="inhA"
FT   promoter        1..162
FT                   /gene="inhA"
FT   CDS             163..906
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="putative ketoacyl ACP reductase;  Method: conceptual
FT                   translation supplied by author."
FT                   /db_xref="GOA:P0A5Y5"
FT                   /db_xref="InterPro:IPR002198"
FT                   /db_xref="InterPro:IPR002347"
FT                   /db_xref="InterPro:IPR011284"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A5Y5"
FT                   /protein_id="AAB60182.1"
FT                   /translation="MTATATEGAKPPFVSRSVLVTGGNRGIGLAIAQRLAADGHKVAVT
FT                   HRGSGAPKGLFGVECDVTDSDAVDRAFTAVEEHQGPVEVLVSNAGLSADAFLMRMTEEK
FT                   FEKVINANLTGAFRVAQRASRSMQRNKFGRMIFIGSVSGSWGIGNQANYAASKAGVIGM
FT                   ARSIARELSKANVTANVVAPGYIDTDMTRALDERIQQGALQFIPAKRVGTPAEVAGVVS
FT                   FLASEDASYISGAVIPVDGGMGMGH"
FT   CDS             925..1734
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="enoyl ACP reductase"
FT                   /function="mycolic acid synthesis"
FT                   /db_xref="GOA:P0A5Y7"
FT                   /db_xref="InterPro:IPR002198"
FT                   /db_xref="InterPro:IPR014358"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A5Y7"
FT                   /protein_id="AAB60183.1"
FT                   /translation="MTGLLDGKRILVSGIITDSSIAFHIARVAQEQGAQLVLTGFDRLR
FT                   LIQRITDRLPAKAPLLELDVQNEEHLASLAGRVTEAIGAGNKLDGVVHSIGFMPQTGMG
FT                   INPFFDAPYADVSKGIHISAYSYASMAKALLPIMNPGGSIVGMDFDPSRAMPAYNWMTV
FT                   AKSALESVNRFVAREAGKYGVRSNLVAAGPIRTLAMSAIVGGALGEEAGAQIQLLEEGW
FT                   DQRAPIGWNMKDATPVAKTVCALLSDWLPATTGDIIYADGGAHTQLL"
FT   variation       1204
FT                   /replace="g"
FT                   /note="causes a change in the encoded protein from serine
FT                   to alanine. Expression of this altered protein in
FT                   Mycobacterium bovis and Mycobacterium smegmatis confers
FT                   isoniazid resistance."
XX
SQ   Sequence 1789 BP; 340 A; 550 C; 602 G; 297 T; 0 other;
     tgcccagaaa gggatccgtc atggtcgaag tgtgctgagt cacaccgaca aacgtcacga        60
     gcgtaacccc agtgcgaaag ttcccgccgg aaatcgcagc cacgttacgc tcgtggacat       120
     accgatttcg gcccggccgc ggcgagacga taggttgtcg gggtgactgc cacagccact       180
     gaaggggcca aacccccatt cgtatcccgt tcagtcctgg ttaccggagg aaaccggggg       240
     atcgggctgg cgatcgcaca gcggctggct gccgacggcc acaaggtggc cgtcacccac       300
     cgtggatccg gagcgccaaa ggggctgttt ggcgtcgaat gtgacgtcac cgacagcgac       360
     gccgtcgatc gcgccttcac ggcggtagaa gagcaccagg gtccggtcga ggtgctggtg       420
     tccaacgccg gcctatccgc ggacgcattc ctcatgcgga tgaccgagga aaagttcgag       480
     aaggtcatca acgccaacct caccggggcg ttccgggtgg ctcaacgggc atcgcgcagc       540
     atgcagcgca acaaattcgg tcgaatgata ttcataggtt cggtctccgg cagctggggc       600
     atcggcaacc aggccaacta cgcagcctcc aaggccggag tgattggcat ggcccgctcg       660
     atcgcccgcg agctgtcgaa ggcaaacgtg accgcgaatg tggtggcccc gggctacatc       720
     gacaccgata tgacccgcgc gctggatgag cggattcagc agggggcgct gcaatttatc       780
     ccagcgaagc gggtcggcac ccccgccgag gtcgccgggg tggtcagctt cctggcttcc       840
     gaggatgcga gctatatctc cggtgcggtc atcccggtcg acggcggcat gggtatgggc       900
     cactgacaca acacaaggac gcacatgaca ggactgctgg acggcaaacg gattctggtt       960
     agcggaatca tcaccgactc gtcgatcgcg tttcacatcg cacgggtagc ccaggagcag      1020
     ggcgcccagc tggtgctcac cgggttcgac cggctgcggc tgattcagcg catcaccgac      1080
     cggctgccgg caaaggcccc gctgctcgaa ctcgacgtgc aaaacgagga gcacctggcc      1140
     agcttggccg gccgggtgac cgaggcgatc ggggcgggca acaagctcga cggggtggtg      1200
     cattcgattg ggttcatgcc gcagaccggg atgggcatca acccgttctt cgacgcgccc      1260
     tacgcggatg tgtccaaggg catccacatc tcggcgtatt cgtatgcttc gatggccaag      1320
     gcgctgctgc cgatcatgaa ccccggaggt tccatcgtcg gcatggactt cgacccgagc      1380
     cgggcgatgc cggcctacaa ctggatgacg gtcgccaaga gcgcgttgga gtcggtcaac      1440
     aggttcgtgg cgcgcgaggc cggcaagtac ggtgtgcgtt cgaatctcgt tgccgcaggc      1500
     cctatccgga cgctggcgat gagtgcgatc gtcggcggtg cgctcggcga ggaggccggc      1560
     gcccagatcc agctgctcga ggagggctgg gatcagcgcg ctccgatcgg ctggaacatg      1620
     aaggatgcga cgccggtcgc caagacggtg tgcgcgctgc tgtctgactg gctgccggcg      1680
     accacgggtg acatcatcta cgccgacggc ggcgcgcaca cccaattgct ctagagcggc      1740
     cgcgcaccgc ggtggagctc aattcgccct atagtgagtc gtattgcgg                  1789
//