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EBI DbfetchID U39940; SV 1; linear; genomic DNA; STD; PRO; 6379 BP. XX AC U39940; XX DT 04-DEC-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Sinorhizobium meliloti bet operon, complete sequence. XX KW . XX OS Sinorhizobium meliloti OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Sinorhizobium/Ensifer group; Sinorhizobium. XX RN [1] RP 1-6379 RX PUBMED; 9141699. RA Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.C., RA Le Rudulier D.; RT "Molecular characterization of the bet genes encoding glycine betaine RT synthesis in Sinorhizobium meliloti 102F34"; RL Microbiology (Reading, Engl.) 143(Pt):0-0(0). XX RN [2] RP 1-6379 RA Osteras M., Boncompagni E., Vincent N., Poggi M.C., Le Rudulier D.; RT "Presence of a gene encoding choline sulfatase in Sinorhizobium meliloti RT bet operon: Choline-O-sulfate is metabolized into glycine betaine"; RL Unpublished. XX RN [3] RP 1-6379 RA Vincent N.; RT ; RL Submitted (03-NOV-1995) to the EMBL/GenBank/DDBJ databases. RL Biologie Vegetale et Microbiologie, URA CNRS 1114, Universite de Nice - RL Sophia Antipolis, Parc Valrose, 06108 Nice Cedex 2, France XX RN [4] RP 1-6379 RA Le Rudulier D.; RT ; RL Submitted (14-APR-1998) to the EMBL/GenBank/DDBJ databases. RL Biologie Vegetale et Microbiologie, Universite de Nice - Sophia Antipolis, RL Parc Valrose, 06108 Nice Cedex 2, France XX FH Key Location/Qualifiers FH FT source 1..6379 FT /organism="Sinorhizobium meliloti" FT /strain="102F34" FT /mol_type="genomic DNA" FT /db_xref="taxon:382" FT CDS 713..1324 FT /codon_start=1 FT /transl_table=11 FT /gene="betI" FT /product="transcriptional regulator" FT /note="BetI" FT /db_xref="GOA:O69786" FT /db_xref="InterPro:IPR001647" FT /db_xref="InterPro:IPR009057" FT /db_xref="InterPro:IPR012287" FT /db_xref="InterPro:IPR017757" FT /db_xref="UniProtKB/Swiss-Prot:O69786" FT /protein_id="AAC13370.1" FT /translation="MPKVGMEPVRRKALVDAALRVIGDQGTLAVTMSEIARTAGVSPAL FT AHHYFGSKEQLLIATIRSLLGKLRDDAVAAMKAAATPRERVSALIRVSFRADQFAPETV FT AAWLAFYSEAQRSEEVRRLLVIYARRLRSNLLVGLRALCPADDAERIAEGAAAMIDGLY FT IRQSLKSAPISIEASVALTEDYVNAHLRANGDGKYPSPRV" FT CDS 1491..3029 FT /codon_start=1 FT /transl_table=11 FT /gene="betC" FT /product="choline sulfatase" FT /function="converts choline-O-sulfate to choline" FT /EC_number="3.1.6.6" FT /note="BetC" FT /db_xref="GOA:O69787" FT /db_xref="InterPro:IPR000917" FT /db_xref="InterPro:IPR017785" FT /db_xref="InterPro:IPR017849" FT /db_xref="InterPro:IPR017850" FT /db_xref="UniProtKB/Swiss-Prot:O69787" FT /protein_id="AAC13371.1" FT /translation="MTTGKPNILIIMVDQLNGKLFPDGPADFLHAPNLKALAKRSARFH FT NNYTSSPLCAPARASFMAGQLPSRTRVYDNAAEYQSSIPTYAHHLRRAGYYTALSGKMH FT FVGPDQLHGFEERLTTDIYPADFGWTPDYRKPGERIDWWYHNLGSVTGAGVAEITNQME FT YDDEVAFLANQKLYQLSRENDDESRRPWCLTVSFTHPHDPYVARRKFWDLYEDCEHLTP FT EVGAIPLDEQDPHSQRIMLSCDYQNFDVTEENVRRSRRAYFANISYLDEKVGELIDTLT FT RTRMLDDTLILFCSDHGDMLGERGLWFKMNFFEGSARVPLMIAGPGIAPGLHLTPTSNL FT DVTPTLADLAGISLEEVRPWTDGVSLVPMVNGVERTEPVLMEYAAEASYAPLVAIREGK FT WKYVYCALDPEQLFDLEADPLELTNLAENPSGPVDQATLTAFRDMRAAHWDMEAFDAAV FT RESQARRWVVYEALRNGAYYPWDHQPLQKASERYMRNHMNLDTLEESKRYPRGE" FT RBS 3020..3026 FT /gene="betB" FT CDS 3031..4494 FT /codon_start=1 FT /transl_table=11 FT /gene="betB" FT /product="betaine aldehyde dehydrogenase" FT /function="converts betaine aldehyde to glycine betaine" FT /EC_number="1.2.1.8" FT /note="BetB" FT /db_xref="GOA:P54222" FT /db_xref="InterPro:IPR011264" FT /db_xref="InterPro:IPR015590" FT /db_xref="InterPro:IPR016160" FT /db_xref="InterPro:IPR016161" FT /db_xref="InterPro:IPR016162" FT /db_xref="UniProtKB/Swiss-Prot:P54222" FT /protein_id="AAC13368.1" FT /translation="MRAQPKASHFIDGEYVEDAAGTVIESIYPATGEIIARLHAATPGI FT VEKAIAAAKRAQPEWAAMSPTARGRILKRAAELMRQLNRELSELETLDTGKPIQETIVA FT DPTSGADSFEFFGGVAPAALNGDYIPLGGDFAYTKRVPLGVCVGIGAWNYPQQIACWKG FT APALVAGNAMVFKPSENTPLGALKIAEILIEAGLPKGLFNVIQGDRATGPLLVNHPDVA FT KVSLTGSVPTGKKVAGAAAAELKHVTMELGGKSPLIVFDDADLESAIGGAMLGNFYSTG FT QVCSNGTRVFVQRKIKEPFLARLKERTEAIVIGDPLDEATQLGPMVSAAQRDKVFSYIG FT KGKAEGARLVTGGGIPNNVSGEGTYIQPTVFADDTDGMTIAREEIFGPVMCVLDFDDEV FT EVIARANATEFGLSAGVFTADLTRAHRVADRLEAGTLWINTYNLCPVEIPFGGSKQSGF FT GRENSVAALNHYTELKTVYVGMGPVEAPY" FT RBS 4692..4696 FT /gene="betA" FT CDS 4705..6351 FT /codon_start=1 FT /transl_table=11 FT /gene="betA" FT /product="choline dehydrogenase" FT /function="converts choline to betaine aldehyde" FT /EC_number="1.1.99.1" FT /note="BetA" FT /db_xref="GOA:P54223" FT /db_xref="InterPro:IPR000172" FT /db_xref="InterPro:IPR007867" FT /db_xref="InterPro:IPR011533" FT /db_xref="InterPro:IPR012132" FT /db_xref="UniProtKB/Swiss-Prot:P54223" FT /protein_id="AAC13369.1" FT /translation="MQADFVIIGSGSAGSALAYRLSEDGRNSVIVLEFGGSDVGPFIQM FT PAALAWPMSMNRYNWGYLSEPEPNLNNRRITAPRGKVIGGSSSINGMVYVRGHSEDFNR FT WEELGAQGWAYADVLPYYKRMEHSHGGEEGWRGTDGPLHVQRGPVKNPLFHAFIEAGKE FT AGFEVTEDYNGSKQEGFGLMEQTTWRGRRWSAASAYLRPALKRPNVELIRCFARKIVIE FT NGRATGVEIERGGRIEVVKANREVIVSASSFNSPKLLMLSGIGPRAHLKEMGIDVKVDR FT PGVGQNLQDHMEFYFQQVSTKPVSLYSWLPWFWQGVAGAQWLFFKRGLGISNQFESCAF FT LRSAPGVKQPDIQYHFLPVAISYDGKAAAKSHGFQVHVGYNLSKSRGNVSLRSSDPKAD FT PVIRFNYMSHPEDWEKFDLRAVTGEIFGEKAFDLYRGPEIQPGEKVQTDEEIDGFLREH FT LESAYHPCGTCKMGAKDDPMAVVDPETRVIGVDGLRVADSSIFPHITYGNLNAPSIMTG FT EKSADHILGRQPLARSNQEPWINPRWAVSDR" XX SQ Sequence 6379 BP; 1268 A; 2072 C; 1992 G; 1047 T; 0 other; aagcttcgga aaaggcctgc tcagcccctc gcaagaaccc cgaaggcgat cgaggccagc 60 ccctgcatga cgagatcgac cgccaggaaa agacccagta tccagagact gtcgaccggc 120 cagccggcgg cgatgatgag gccggctagc agcgtcacga ggccgccgat gaccatccac 180 ccccagccgc cgagcggccg aagcctgaac cctacccaga tgcgggagca accggcggcg 240 atcagcgcca gggccagcag aaacgtcagc acggaggagg cgaggacagg attgatgaag 300 gcgaacaggc ccgctgcggc gtagaaggcg ccgctcaata tccagtaaag gacactcccc 360 cagcttttga cctgaaaggc gtgggcgaga ttgagcaggc cgccgatcag catgatgaga 420 ccgacgtaat agacggaggc gactgtcgcc atcagcaggt ttccgaaggc gatgccgccg 480 cacatgatca gaagcacgcc gagtgcgacg aaccaccccc attttccggc aagggcctct 540 ctgccggctg gatcggacgc gtgaaccatt tcaacctcca gtcatgccga tgcgcatgga 600 cgcctcccgc tgattgagcc ggatttccaa gcaaaaagaa agagaaaagc gcagattttt 660 ttattgattg atggatcaat caaaaataag ctggcacctg aggagttcga agatgccgaa 720 ggtcggaatg gagccggtgc gccgcaaggc gctggtcgat gcggcgctgc gcgtgatcgg 780 cgatcagggt acgctcgcgg tgaccatgtc ggagatcgcg cggacggccg gcgtctcacc 840 cgcgctcgcg catcactatt tcggcagcaa ggagcagttg ctgatcgcca cgatccgcag 900 cctcctgggc aaattgcgcg acgacgccgt cgcggcaatg aaagcggcgg cgacgccgcg 960 cgaaagggtg agcgcgttga tccgcgtgag cttccgcgcc gatcagttcg cgccggagac 1020 ggtcgccgcc tggctcgcct tctattcgga ggcgcaacgg tcggaggagg tgcggcggct 1080 gctcgtgatc tatgcacggc ggctgcgctc caatctgctt gtgggcctga gagcgctctg 1140 cccggcggac gacgcggagc gcatcgccga aggcgcggct gcgatgatcg acgggctcta 1200 catccggcaa agtttgaagt cggcgccgat cagcatcgaa gcatcggtcg cgctcacgga 1260 agattatgtg aatgcgcatt tacgggcgaa tggtgacggg aaatacccct ctcctcgggt 1320 ttaacccgag gactaaccct ctccccgcgt gcggggagag ggaacttcgg cagcaccgca 1380 tgtcccttct ccccgccggc ggggagaagg tggcggcagc cggatgaggg gcaatttcca 1440 cctgaaccaa gcaagacgag caagaattcg aaccatcgag gaaccgacca gtgaccaccg 1500 gtaagcccaa cattctgatc atcatggtgg accagttgaa cggaaagctc tttccggacg 1560 ggcctgcaga cttcctgcat gcgcccaacc tgaaggcgct ggccaagcgg tcggcgcgtt 1620 tccacaacaa ctacacctcg tcgcccctgt gtgcccctgc ccgcgcatcc ttcatggccg 1680 ggcaattgcc gagccgcacc cgggtctacg acaatgctgc cgaataccag tcgtcgatcc 1740 caacctatgc acatcacctg cgccgggccg gctactacac cgcgctttcc ggcaagatgc 1800 atttcgtcgg gccggaccag ttgcacggct tcgaggagcg gctgacgacc gacatctatc 1860 ccgccgattt cggctggacg ccggattacc gcaagccggg cgagcggatc gactggtggt 1920 atcacaatct cggctccgtc accggcgccg gggtcgccga aatcaccaac cagatggaat 1980 atgacgacga ggtggccttc ctcgcaaacc agaagctcta ccagctttcg cgggagaacg 2040 acgacgagag ccggcgtccc tggtgcctca ccgtctcctt cacccacccg cacgacccct 2100 atgtcgcgcg gcggaaattc tgggatctct acgaggattg cgagcacctc acgcccgagg 2160 tcggcgccat tcccctcgac gagcaggacc cgcattcgca acggatcatg ctctcctgcg 2220 actaccagaa tttcgacgtg accgaggaga atgtccgccg ctcgcgccgc gcctatttcg 2280 ccaatatctc ctatctcgac gagaaggtgg gcgagctgat cgacacgctc acgcggacgc 2340 ggatgctcga cgatacgctc atcctcttct gttccgacca cggcgacatg ctcggcgagc 2400 gcggcctctg gttcaagatg aacttcttcg aaggctcggc gcgcgtgccg ctgatgattg 2460 ccggcccggg tatcgcaccc ggcctgcatc tgacgccgac ctccaatctc gacgtgacgc 2520 cgacgcttgc ggacctcgcc ggcatttcgc tcgaggaagt gaggccctgg accgatggcg 2580 tcagcctcgt gccgatggtc aacggcgtcg aacgcacgga gccggtgctg atggaatacg 2640 cggctgaggc ctcctatgca ccgctggtcg ccatccgcga gggcaagtgg aaatatgtct 2700 actgcgcgct cgatccggag cagctcttcg atctcgaggc agatccgctg gagcttacga 2760 acctcgcgga aaatccgagc gggcccgtcg atcaggcgac gctcacggca ttccgcgaca 2820 tgcgcgcggc gcattgggac atggaagcct tcgacgctgc cgtgcgcgag agccaggcac 2880 ggcgctgggt ggtctacgaa gcgcttcgca acggcgccta ctacccctgg gaccaccagc 2940 cgctgcaaaa ggcatcggag cgctacatgc gcaaccacat gaacctcgac actctcgagg 3000 aatccaaacg ctatccgcga ggagaatgag atgagagccc aaccgaaagc ctcgcacttc 3060 atcgacggcg aatatgtcga ggacgccgcc ggcacggtga tcgagagcat ctatccggcg 3120 accggcgaaa tcatcgcccg gctccatgcc gcaacgcctg ggatcgtcga gaaggcgatc 3180 gccgcggcca agcgcgcgca gccggaatgg gcggcgatga gcccgacggc gcgcggccgc 3240 atcctgaagc gggccgccga gctcatgcgc cagctcaacc gcgagctttc cgaactcgaa 3300 acgctcgaca ccggcaagcc gatccaggaa accatcgtcg ccgacccgac gtccggcgcc 3360 gacagtttcg aattcttcgg cggcgtcgcg cccgccgcac tcaacggcga ttatatcccg 3420 ctcggcggcg acttcgccta tacgaagcgg gtgccgctcg gcgtctgcgt cggcatcggc 3480 gcctggaact atccgcagca gatcgcctgc tggaagggtg cgcctgcgct cgtcgccggc 3540 aatgcgatgg tgttcaagcc ttcggaaaac accccgctcg gcgcgcttaa gatcgccgaa 3600 atccttatcg aagcgggtct gccgaagggc ctgttcaacg tcatccaggg cgaccgcgcg 3660 acgggcccgc tcctcgtcaa ccatccggac gtcgccaagg tgtcgctcac cggctccgtg 3720 ccgacgggca aaaaagtcgc gggcgccgcg gcggccgaac tcaagcacgt caccatggag 3780 ctcggcggca agtcgccgct gatcgtcttc gacgatgccg atctcgaaag cgcgatcggc 3840 ggcgccatgc tcggcaattt ctattcgacc ggccaggtct gctccaacgg cacgcgcgtc 3900 ttcgtgcaaa ggaagatcaa ggagcctttc ctcgcccgcc tcaaggaacg caccgaagcg 3960 atcgtcatcg gcgaccccct ggacgaggcg acgcagctcg gtccgatggt ctcggcggcg 4020 caacgcgaca aggtcttctc ctatatcggg aagggcaagg cggaaggcgc ccgcctcgtc 4080 accggcggcg gcattcccaa taatgtgagc ggcgaaggca cctatatcca gccgaccgtc 4140 ttcgccgacg acaccgacgg gatgacgatc gcgcgcgaag aaatcttcgg accggtcatg 4200 tgcgtgctcg acttcgacga cgaggtggaa gtcatcgcac gcgccaacgc caccgaattc 4260 ggcctttcgg ccggcgtctt caccgcggac ctcacccgcg cccaccgggt cgccgaccgg 4320 ctcgaagccg gcacgctctg gatcaacacc tacaatctct gcccggtaga gatccccttt 4380 ggcgggtcca agcaatcggg tttcgggcgg gagaattcgg tcgcggcgct caaccactat 4440 accgagctca agaccgtcta tgtcggcatg gggccggtcg aggcgccgta ttgaaagttg 4500 gcgcttgccc ctctcctcgg gtttaacccg aggactaacc ctctccccgc tcgcggggag 4560 aggggaccgg aggcagcgag cttccgcgcc ggcggcagag ggactgagcg ggcgccgcgt 4620 gtccttctcc ccgcgagcgt ggagaaggtg gcggcagccg gatgaggggc atgccgagtg 4680 catccaaaaa agaaagcagc gagaatgcag gcagatttcg tcatcatcgg ttccggctcg 4740 gcgggctccg cgctcgccta tcgcctgtcg gaagacggca ggaattcggt catcgtgctc 4800 gagttcggcg gctcggatgt cggtccgttc atccagatgc cggcagcgct tgcctggccg 4860 atgagcatga accgctataa ttggggctat ctctccgaac ccgagccgaa cctcaacaac 4920 aggcgcatca ccgcgccgcg cggcaaggtg atcggcggct cctcctcgat caacggcatg 4980 gtctatgtcc gcggccactc ggaagacttc aaccgctggg aggaactcgg cgcacaaggc 5040 tgggcctatg cggacgtgct gccctattac aagcggatgg agcattcgca tggcggcgaa 5100 gagggctggc gcggcaccga cgggccgctg cacgtccagc gcggcccggt caagaatccc 5160 ctttttcacg ccttcatcga agcgggaaaa gaggccggct tcgaagtcac cgaggattat 5220 aacggctcga agcaggaggg cttcgggctg atggagcaga cgacatggcg gggccgccgc 5280 tggtcggccg cttccgccta tctgaggccg gcgctcaaac gtccgaatgt cgagctcatc 5340 cgttgcttcg cccgcaagat cgtcatcgag aacggccggg cgacgggcgt ggagatcgag 5400 cggggcggac ggatcgaggt ggtgaaggcc aatcgcgaag tgatcgtctc cgcctcttcg 5460 ttcaattcgc cgaagctgct gatgctgtcc ggcatcggcc cgcgtgcgca cctcaaggag 5520 atgggaattg acgtaaaggt cgaccggccc ggcgtcggcc agaacctgca ggaccacatg 5580 gaattctatt tccagcaggt gagcacgaag ccggtttcac tctattcctg gctgccatgg 5640 ttctggcagg gcgtcgccgg tgcgcaatgg ctcttcttca aaagaggcct cggcatttcc 5700 aaccagttcg agtcctgcgc cttcctgcgc tcggcacccg gcgtgaagca gccggacatc 5760 caataccact tcctgccggt ggcgatcagc tatgacggca aggcggcggc gaagtcgcac 5820 ggcttccagg tgcatgtcgg ctacaacctc tccaagtcgc gcggcaacgt ctcgctccgc 5880 tcctccgacc cgaaggccga cccggtgatc cgcttcaact atatgagcca tccggaggac 5940 tgggagaagt tcgacctgcg tgcggtcacg ggggagattt tcggggagaa ggctttcgac 6000 ctctatcgcg gccccgaaat ccagcccggc gaaaaggtgc agacggacga ggagatcgac 6060 gggttcctgc gcgagcatct cgaaagcgcc tatcatccct gcggcacctg caagatgggt 6120 gcgaaggacg acccgatggc cgtggtcgac ccggaaaccc gggttatcgg cgtcgacggg 6180 cttcgtgtcg ccgactcctc gatcttcccg cacatcacct atggcaacct gaacgccccc 6240 tcgatcatga ccggcgagaa gtccgccgat catatcctcg gcagacagcc gctcgcccgc 6300 tcgaaccagg agccgtggat caatccgcgc tgggcggtga gcgacaggta acttattccg 6360 agaggaaacc gatccagcg 6379 // |
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