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EBI DbfetchID U39812; SV 1; linear; genomic DNA; STD; PRO; 3900 BP. XX AC U39812; XX DT 23-AUG-1996 (Rel. 49, Created) DT 23-AUG-1996 (Rel. 49, Last updated, Version 1) XX DE Caldicellulosiruptor saccharolyticus beta-1,4-mannanase (manA) gene, DE complete cds. XX KW . XX OS Caldicellulosiruptor saccharolyticus OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor. XX RN [1] RP 1-3900 RX DOI; 10.1016/0378-1097(96)00201-7 RX PUBMED; 8764509. RA Gibbs M.D., Elinder A.U., Reeves R.A., Bergquist P.L.; RT "Sequencing, cloning and expression of a beta-1,4-mannanase gene, manA, RT from the extremely thermophilic anaerobic bacterium, Caldicellulosiruptor RT Rt8B.4"; RL FEMS Microbiol. Lett. 141(1):37-43(1996). XX RN [2] RP 1-3900 RA Gibbs M.D.; RT ; RL Submitted (31-OCT-1995) to the EMBL/GenBank/DDBJ databases. RL Moreland D. Gibbs, School of Bio Sci, Macquarie University, Sydney, NSW RL 2109, Australia XX FH Key Location/Qualifiers FH FT source 1..3900 FT /organism="Caldicellulosiruptor saccharolyticus" FT /strain="Rt8B.4" FT /mol_type="genomic DNA" FT /note="thermophilic anaerobe" FT /db_xref="taxon:44001" FT CDS 819..3554 FT /codon_start=1 FT /transl_table=11 FT /gene="manA" FT /product="beta-1,4-mannanase" FT /EC_number="3.2.1.78" FT /db_xref="GOA:P77847" FT /db_xref="InterPro:IPR013781" FT /db_xref="PDB:1PMH" FT /db_xref="UniProtKB/TrEMBL:P77847" FT /protein_id="AAC44232.1" FT /translation="MGVCLDMWRNWRVLFVLVTIIFLFSLIPTGIFGAVESSVNPVVLD FT FEDGTVMSFGEAWGDSLKCIKKVSVSQDLQRPGNKYALRLDVEFNPNNGWDQGDLGTWF FT GGVVEGQFDFTGYKSVEFEMFIPYDEFSKSQGGFPYKVVINDGWKELGSEFNITANAGK FT KVKINGKDYTVIHKAFAIPEDFRTKKRAQLVFQFAGQNSNYKGPIYLDNVRIRPEDASN FT LSKEDYGEQAEEGVAVEDFFTGVKLVYPEQGKSFVYNFEIDTMGFYKYSGDGFNKKTKS FT LEYSQDLKRSGNNGALKVNASLAGTAFDEMNIAIKLTDKDDKKFDFSKYSTLEYTLYIP FT NPDKISGKLMVASAIDNPWQIIKDFTAINYKDKNAIQKINGKDYAVIKCSDNLYNVNTK FT ANVLVLRIAGSYVKYTGPIYIDNVKLVAGKKVAPKVKTTSSIPNIKNYYRVKIEAETAK FT DGWAYSIEKENAKYSGKGYILLFGNNMGNTLYDLKIPKTGYYIFTISSSTLGMVNYGSV FT DIWIDGELKGAAKVPNVKGKFQEVVVMKKIYLTQGEHTLSLQKSGGYTIAIDYFTIEEL FT VLANKNKISVDTKLVTPNPHPNAQKLMKYLASIYGEKILSGQQSSGDGKEIQMIFDVTK FT RYPAVRGFDFMDYSPSRVEHGTKGTDVEEAIKWWKSGGIVAFCWHWNAPTGLIDQPGKE FT WWRGFYTEATTFDIKKAMDNPNSTEYKLILRDIDAIAEQLKRLQKEGVPVLFRPLHEAS FT GGWFWWGAKGPEPYIKLWKLMFDRLVNYHKLNNLIWVWNGQDAAWYPGDNYVDIIGEDI FT YEEKAQYSPYADRFAKALKYTNARKMIALTECGTIPDPAVLKQEGISWSWFSVWAGNFV FT MTGSKYNDEWNDNHMLRKIYNSDYVITKDELPDIKNIPLK" XX SQ Sequence 3900 BP; 1346 A; 630 C; 864 G; 1060 T; 0 other; gatccgggta ttaagagaag accaaaatac ctgcaggatt ttaatactca gcttgcaaag 60 tattcattct atccggggtc accggactct gttttgggta atttggtatg ggatataaat 120 ggtgcgcttt tcaatgcagc atctgtttct tcagcattgg ctggtttaaa accacaggca 180 gaacaggcag tgagagaggc aaatgctcaa cctgaaaaac cagtattcag accatttaag 240 ccatttacaa ttgactttga aaatggaaag attgacatgt tcacagtact tgacacaaca 300 aagaaaacag tcaaactttc acttgtatct ggcaaagagg ctataaaggg caagtcttta 360 aaggttacat gcgactctaa aaaagatggt gagtggattg acgcactcta tacactgcca 420 agcaaggtaa aaatttatgg ttggcatgat tacaaggtga gctttgatgt aaaacttctt 480 aagaacccac caaaaccggg tgacacgtat atctatgcgc agattataaa cagcaacaaa 540 tctggttata agtcatatgg atggataacc ttcaagcttg acaaggcaaa cacagtatat 600 catgtagaag gtacaattgc tggtattcct gacaactcaa caacaatggg cttaaagatt 660 ggtatccact ggggtgctga ttttttaatt aataacatca aagtagaaga ggtaactcag 720 taaaaggcac tggaatctgg tgggcaggca tatccacatt gccacaagaa attttatttt 780 tgacctgtcc accaggtttc aaataaaaaa ccaataaaat gggggtatgt ttagatatgt 840 ggagaaattg gagagtgctt tttgttcttg ttacaatcat atttttattt agtctcattc 900 ccactgggat atttggtgct gttgagtcgt ctgtaaatcc agtagtgctg gactttgaag 960 atggcactgt aatgagcttt ggtgaagctt ggggggattc attaaagtgc ataaaaaagg 1020 tttctgtttc gcaggacctt caaagaccag ggaacaaata tgcattaagg cttgatgttg 1080 agtttaaccc aaacaatggc tgggaccaag gcgaccttgg tacctggttt ggcggtgttg 1140 tagaaggtca gtttgacttt acaggctaca aatctgttga gtttgagatg ttcattccat 1200 acgatgagtt ttctaaatcc caaggcggtt ttccttacaa agttgtcata aatgacgggt 1260 ggaaagagct tggaagtgag tttaacataa ctgcaaatgc aggcaaaaag gttaagataa 1320 atggcaaaga ttacacagtc attcacaagg catttgcaat cccagaagat tttagaacca 1380 aaaaacgtgc acagcttgtc ttccagtttg cagggcaaaa ttcaaactac aaaggaccta 1440 tctatcttga caatgtaaga ataagacctg aggatgcttc aaatctgtca aaagaggact 1500 atggagaaca ggctgaagaa ggagtagcag ttgaagattt cttcacaggt gtgaaactcg 1560 tatatcctga acagggtaag tcttttgttt acaactttga aattgacaca atgggatttt 1620 ataagtacag cggcgatggt ttcaacaaaa agacaaaatc cttagaatat tcacaggatt 1680 taaagagaag tgggaataac ggcgcgctga aagtcaacgc aagccttgct ggtactgcct 1740 ttgatgagat gaacattgct ataaagctca cagacaaaga cgataaaaaa tttgatttta 1800 gcaagtattc tactttggag tacactctct atattccaaa ccctgataag atttctggca 1860 agcttatggt tgcaagtgca atagataatc catggcagat tattaaagac ttcacagcta 1920 ttaattacaa ggataagaac gccattcaaa agataaatgg aaaagactat gcggtaatta 1980 aatgttcaga caatctttac aatgtaaata caaaggcaaa tgttttggtt ttaagaattg 2040 cagggtcata tgtcaaatat acaggaccta tttacattga caatgtaaaa cttgtggcag 2100 gaaagaaggt tgctccaaaa gtgaaaacaa caagcagtat tccaaatatc aagaactatt 2160 acagggtaaa aattgaagcg gaaacagcta aggatgggtg ggcatacagt attgaaaaag 2220 aaaatgcaaa gtattctgga aaaggatata ttcttttgtt tgggaacaat atgggcaata 2280 ctctctatga tttaaagata ccgaaaacag gatactatat attcacaatc agcagctcta 2340 cgcttggtat ggtaaactat ggtagtgttg atatatggat agatggtgaa ttaaagggag 2400 cagcaaaggt tccaaatgtt aaaggtaaat tccaggaagt tgtggtaatg aaaaagatat 2460 atcttacaca aggtgaacat actctgtcac ttcaaaaatc cggtggatat acgattgcaa 2520 tagactactt tacaattgaa gagcttgttt tggcaaacaa gaataaaata agtgttgata 2580 caaagcttgt aacaccaaat ccacatccta atgcgcaaaa gcttatgaaa tacctggcaa 2640 gcatatatgg tgaaaagatt ctctctgggc aacagagcag tggtgatgga aaagaaattc 2700 aaatgatatt tgatgttaca aaaagatatc ctgctgtcag gggctttgac tttatggact 2760 actcaccaag cagggtagag catggcacaa aaggtactga tgtagaagag gcaataaaat 2820 ggtggaagag cggtggaatt gttgctttct gctggcattg gaatgcacca acagggctta 2880 ttgaccagcc gggcaaagaa tggtggagag gattttacac agaagcaaca acatttgata 2940 taaagaaggc tatggacaat cctaattcaa ctgagtataa gctcattttg agagatatag 3000 acgcaattgc tgagcagctc aagaggcttc agaaagaagg agtcccagtt cttttcagac 3060 cgctgcacga agcttcaggt ggctggttct ggtggggggc aaaaggaccc gagccctata 3120 tcaaactctg gaaacttatg tttgataggc ttgtaaatta tcacaagtta aacaacctca 3180 tctgggtatg gaacggtcag gatgcagcat ggtatccggg ggacaactat gttgatataa 3240 ttggcgaaga catttatgag gaaaaagctc aatattcccc ctatgcagac aggtttgcaa 3300 aggcattgaa gtacacaaat gcaagaaaga tgatagcact tactgagtgt ggaacaattc 3360 cagacccagc agtattaaaa caggaaggta tttcatggag ctggttctct gtctgggcag 3420 gaaactttgt catgacaggc agcaagtata atgatgagtg gaatgacaat catatgctaa 3480 gaaagattta caatagcgac tatgtaataa caaaagacga acttcctgat attaagaaca 3540 taccattaaa gtaagattaa aaaattgagt agaaacaaag atggagtttt tataagagga 3600 gagggaggag ttttgttagg ttgatagctt tcttctttct cttttatttt ttaagttatt 3660 atatagaaaa gtcaaaacaa tatttgcctt tgacatatca accaatgtaa attaaaatta 3720 aggtatagtg ttttgcatac tcaacataat atatcattaa tatccttttt gttttttgca 3780 agggggtgtt tttgtggcgg ggcacagtaa aaaaagcagc cacagtgcca cagccataat 3840 aacaagggaa gaaaaagccc gatttgttcc caaaagcagc aatgtgcata ataaaagctt 3900 // |
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