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EBI DbfetchID U39070; SV 1; linear; genomic DNA; STD; PRO; 6016 BP. XX AC U39070; XX DT 08-JUN-1996 (Rel. 48, Created) DT 15-FEB-1999 (Rel. 58, Last updated, Version 7) XX DE Fibrobacter succinogenes endoglucanase F precursor (celF) and P2 genes, DE complete cds, P3 precursor gene, partial cds. XX KW cellulose-binding protein; glucanase. XX OS Fibrobacter succinogenes OC Bacteria; Fibrobacteres; Fibrobacterales; Fibrobacteraceae; Fibrobacter. XX RN [1] RP 1-6016 RA Malburg L.M.Jr., Forsberg C.W.; RT "Fibrobacter succinogenes S85 possesses at least nine distinct glucanase RT genes"; RL Can. J. Microbiol. 39:882-891(1993). XX RN [2] RP 1-6016 RX PUBMED; 9172367. RA Malburg S.R., Malburg L.M.Jr., Liu T., Iyo A.H., Forsberg C.W.; RT "Catalytic properties of the cellulose-binding endoglucanase F from RT Fibrobacter succinogenes S85"; RL Appl. Environ. Microbiol. 63(6):2449-2453(1997). XX RN [3] RP 1-6016 RA Malburg L.M.Jr.; RT ; RL Submitted (21-OCT-1995) to the EMBL/GenBank/DDBJ databases. RL Section of Molecular and Cellular Biology, University of California, Davis, RL Briggs Hall R239, Davis, CA 95616, USA XX CC On Nov 5, 1996 this sequence version replaced gi:1633567. XX FH Key Location/Qualifiers FH FT source 1..6016 FT /organism="Fibrobacter succinogenes" FT /mol_type="genomic DNA" FT /note="cloned in pCel6 and LCel6" FT /db_xref="taxon:833" FT -35_signal 317..322 FT /gene="celF" FT -10_signal 349..357 FT /gene="celF" FT RBS 371..376 FT /gene="celF" FT CDS 385..3546 FT /codon_start=1 FT /transl_table=11 FT /gene="celF" FT /product="endoglucanase F precursor" FT /EC_number="3.2.1.4" FT /note="cellulose-binding endoglucanase F, EGF" FT /db_xref="GOA:P77865" FT /db_xref="InterPro:IPR005087" FT /db_xref="UniProtKB/TrEMBL:P77865" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC45377.1" FT /translation="MIKQSLKVASLAVLGLSVTAAMAQPKRPHLAVYKFFDEQYRPGGY FT DYSYGGTSKGVTITKSGGYKSKAALNIKLDPKEYSGASICLYNEFFDLNKYMLDSKVEF FT MIKGKNGGESVKVGLLDEEVSDGKKTQVVLPMNKYIEGGAVTTDWKKVSIPLVDFPDRG FT LYWDNTRKSEFPSRIDWDKIAEIRFSIDKSAASEFEVWVDNIEIVKGNKKAAPKKQMVY FT WDENNDIIDGPKNPEKLDGKAKTLATFYDNQVKGFSYSYGGLTAQREAQSKTPGNKNVL FT AMYIDNNDWSGVTYSLGEGKFIDLSKVRDKGGLYFWIKGKLGGEKLYVGILDNQGNDIK FT SQTKVGLNDWIKVSKDWQLAKIPLKRFTDKGKAWDANKSAEVAKDIKWDKIQEIRFSVG FT KGENAGEPGKPAPVTVFVDQITFTSNIDWVDPDLKWDSFKSNAPDYVISDFEGKFAKDK FT WEPSTGPKSQLKFKVENCAEFKSNCLNIEHYLLADWVDVVLDMQKNGRPAADRDWTKHW FT GIMFDVYSEKAWQSITVQVQDAGNEIFVSNVGAPKGKTTILVPFRTFGKFPYYQPPNAV FT ENGLFDLKGVTALDFKPSGEGTAGGFKVDNIRLTNQREVKAKERPAVIKVLVKGEKDVL FT NPNISGGLFGINAALWDGDMLDNKNFKVQTREYAKRINHGIIRYPGGLRADDDHWKEIL FT DNHDWMVDTDEFLEWLKKTGSNAMFTVNFGSGTVKEAADWVKHTNIDKKAGILYWEIGN FT EIYGNWHPYYEKYGKDGGTIYGKRARKFIEAMKKVDPTIKVAVLGVLEGDWNDKVLAET FT GDIADGLIVHHYPQHFGEENDFAMLSAPQTLTAIYERLHKVVDKWTKKYNKDKKIELWL FT TEWNSVDFNPGPQTLSVENGLFVADYLGMLATENVDNAQYWDIHNDITPEGGDYGYLTR FT SGEECMNCPRPSYWAFQMASDALRGKLMKTTIKGDEDALLTAYWTVNGNKKQLLLVNKS FT PYSEFDIKLDIPGFKGKAKVQTLDKTSEKLKEGWANDPSKKAKTVDISKGIKVGKRTLT FT LITLE" FT mat_peptide 454..3441 FT /gene="celF" FT /product="endoglucanase F" FT CDS 4138..4893 FT /codon_start=1 FT /transl_table=11 FT /product="P2" FT /note="ORF2; hypothetical" FT /db_xref="UniProtKB/TrEMBL:P71327" FT /protein_id="AAC45378.1" FT /translation="MEESLREIPDESDRAFIYIVLNSMFRYKEDVSSMIENYKYQLTDE FT KQLYFLVNRFWHKENYDSKKYFAFSWGGAVIKSFGRLSDKIGLSPGMYLGIDFVRKDFL FT YEWFMDINGCQNKELDSLQFYDMRWDFNFGYTFLKKDHLNLFGYVSAGLGMNGLSARGK FT DSNDKANNDLPIQFSPAFGAGVMVDLFFTEKKHIHHGLRFRTGVRSLFSGDVLKTSGVR FT LYASLEWTFREYTKKPIDFDYSFRESGVK" FT -35_signal 4822..4827 FT /note="ORF3" FT -10_signal 4864..4867 FT /note="ORF3" FT RBS 4878..4882 FT /note="ORF3" FT sig_peptide 4890..4931 FT /note="ORF3" FT CDS 4890..>6016 FT /codon_start=1 FT /transl_table=11 FT /product="P3 precursor" FT /note="ORF3; promoter functional in E. coli" FT /db_xref="UniProtKB/TrEMBL:P71328" FT /protein_id="AAC45379.1" FT /translation="MMARWFLMMLGVMVSLSFAARYTRYNIVLSDSSVGYSQSQTVAQK FT LQTAPIAYYGSLIQRTYLQKDFYNAENAEFEQRYPRVYESFVGWANYKQKRQDNLLTQA FT PEQYQKMFEQRYALMNIKGFFLIHDEIRVEKIYDKATHTWVTPKPEDAKKELFSFAYVG FT PDISQDSSWIISQRMMVLLPNSEDQTVPVKRIFFMDGYSEECYSLMDTSILDKIHYVPH FT RLKYDVKPTAAMSAREKMQAFVESGDFASAGALAESADLSPRSKILMKILNKEYDFIVN FT EDSTGLYLDDYNGVVYTDRLDDALNDAAMHEYVSGRYCKSLQDNPDVDSALVCKAVKKV FT ALREMPKPVVHWMGPIRFNAYADFGRPFLAGDFPD" FT mat_peptide 4932..>6016 FT /product="P3" XX SQ Sequence 6016 BP; 1599 A; 1357 C; 1525 G; 1535 T; 0 other; tcagaagcag ggcaagttct gggaataccg ctatgctctc gcaccgcact cccgcgaact 60 ctccgattcc gtctacctcg ctgttgctaa ggaaattggc ctcaacatcg aacagttcaa 120 gaaggacatg gttctcgatt ctgctatgaa cgctcgcatc gacaaggact tccagttggg 180 cgtcaaggtc ggcgttcagg gaactccgaa cttctacatc aacggcaagc gtcaggaccg 240 cttcagcccg gatctcgtcg aaaagatgtt gaaggaagcc aagtaattac gttttcaggc 300 gtgcacgtta aatcgattga cgtgtgcgct ttttaatttc caagtttgta atttaaaatc 360 aaaaacaaca aaaggatgca cattatgatt aagcaatcat tgaaagttgc ctcgctcgcc 420 gtcctcggct tgagcgttac agctgcaatg gctcagccga aacgtccgca tctggctgtg 480 tacaagttct tcgatgaaca gtaccgtccg ggtggatacg attactctta cggcggcacg 540 agcaagggtg tcaccatcac caagtctggt ggttacaagt ccaaggctgc cttgaacatc 600 aagttggacc cgaaggaata ctccggtgct tccatctgcc tttacaatga attcttcgac 660 ttgaacaagt acatgcttga ctccaaggtc gaattcatga tcaagggcaa gaacggtggc 720 gaatctgtta aggtcggcct cctcgacgaa gaagtttctg acggcaagaa gactcaggtc 780 gtgcttccga tgaacaagta catcgaaggc ggcgccgtga cgacggactg gaagaaggtt 840 tccattcctc tcgtggactt cccggaccgt ggtctctact gggacaacac ccgcaagtcc 900 gaattcccgt ctcgtattga ctgggacaag attgctgaaa tccgtttctc cattgacaag 960 agcgctgcaa gcgaattcga agtctgggtc gacaacatcg aaatcgtgaa gggcaacaag 1020 aaggctgccc cgaagaagca gatggtctac tgggacgaaa acaatgatat cattgacggc 1080 ccgaagaacc cggaaaagct cgacggcaag gccaagacgc tcgctacgtt ctatgacaac 1140 caggtcaagg gcttctctta cagctacggt ggtctcaccg ctcagcgtga agctcagtcc 1200 aagactccgg gcaacaagaa cgtgctcgcc atgtacatcg ataacaacga ctggtctggc 1260 gtgacctact ctctcggtga aggcaagttc attgacctct ccaaggttcg cgacaagggc 1320 ggtctctact tctggatcaa gggtaagctc ggtggcgaaa agctctacgt cggtatcctc 1380 gacaaccagg gcaacgatat caagagccag accaaggtcg gcctcaacga ctggatcaag 1440 gtctccaagg attggcagct cgccaagatt cctctcaagc gcttcaccga caagggtaag 1500 gcttgggacg ctaacaagtc cgctgaagtc gctaaggaca tcaagtggga caagattcag 1560 gaaatccgct tctctgttgg caagggcgaa aacgcaggcg aaccgggcaa gccggctcct 1620 gtgacggtct tcgtggacca gatcaccttc acgtccaaca tcgactgggt ggacccggat 1680 ctcaagtggg attccttcaa gtccaacgct ccggactacg tgatctccga cttcgaaggc 1740 aagtttgcta aggacaagtg ggaaccgtcc accggtccga agtctcagct caagttcaag 1800 gttgaaaact gcgccgaatt caagagcaac tgcttgaata tcgaacacta cctccttgct 1860 gactgggttg acgttgtgct cgacatgcag aagaatggcc gtccggctgc tgaccgtgac 1920 tggaccaagc actggggcat catgttcgac gtttactctg aaaaggcatg gcagtccatc 1980 accgttcagg ttcaggatgc tggcaacgaa atcttcgttt ctaacgtcgg tgctccgaag 2040 ggcaagacca caatcctcgt tccgttccgt acgttcggca agttcccgta ctatcaaccg 2100 cctaacgctg ttgaaaacgg tctcttcgac ctcaagggtg taactgctct cgacttcaag 2160 ccgagtggtg aaggtactgc aggtggcttc aaggttgaca acattcgttt gaccaaccag 2220 cgcgaagtca aggctaagga acgtccggct gtcatcaagg tcttggtgaa gggcgaaaag 2280 gatgtcctca acccgaatat ctctggtggc ttgttcggta tcaacgccgc tctctgggac 2340 ggcgacatgc tcgacaacaa gaacttcaag gttcagactc gcgaatacgc aaagcgcatc 2400 aaccacggca ttatccgtta cccgggtggt ctccgtgctg atgacgacca ctggaaggaa 2460 atcctcgaca accacgactg gatggtcgac accgacgaat tcctcgaatg gttgaagaag 2520 actggctcta acgctatgtt cactgtgaac ttcggttccg gcactgtaaa ggaagccgct 2580 gactgggtca agcacacgaa catcgacaag aaggccggca tcctctactg ggaaatcggt 2640 aacgaaatct acggtaactg gcatccgtat tacgaaaagt atggtaagga cggcggtacc 2700 atctatggta agcgcgctcg taagttcatc gaagccatga agaaagttga cccgaccatc 2760 aaggtggctg tgctcggcgt tctcgaaggc gactggaacg acaaggtcct tgctgaaacg 2820 ggtgacatcg ctgacggtct tatcgtccac cactacccgc agcacttcgg cgaagaaaac 2880 gacttcgcta tgctctctgc tccgcagacc cttaccgcaa tctacgaacg tttgcacaag 2940 gtcgtggaca agtggaccaa gaagtacaac aaggataaga agatcgaact ctggctcacc 3000 gaatggaact ctgttgactt caacccgggt ccgcagacct tgtctgtcga aaacggcttg 3060 ttcgtcgctg actacctcgg tatgctcgct actgaaaacg tcgacaacgc tcagtactgg 3120 gatatccaca acgacatcac tccggaaggc ggtgactatg gttacttgac ccgttctggt 3180 gaagaatgca tgaactgccc gcgcccgagc tactgggcat tccagatggc ttccgacgct 3240 ctccgtggca agctcatgaa gaccaccatc aagggtgacg aagacgctct cctgaccgct 3300 tactggactg taaacggcaa caagaagcag ctcctcctcg tgaacaagag cccgtacagc 3360 gaattcgaca tcaagcttga cattccgggc ttcaagggta aggccaaggt ccagactttg 3420 gacaagacct ccgaaaagct taaggaaggc tgggcaaacg acccgtccaa gaaggctaag 3480 actgtcgata tctctaaggg tatcaaggtc ggcaagcgca cccttactct catcactttg 3540 gaataagtga acaagggttc gtaagctaga ttctagaaat cttgaaaagg actccgtcgg 3600 aaacggcgga gttctttttt gtatattgac tttatgcttc atcgattttt gcttattgtt 3660 attcttttgt ctgttgttgt cttgggccgg ccatcgtctg caagcgatgt tccgtttgat 3720 tccgtaatgt atttgtttgt gcaaaatccc gatacaaata cttcaaaaaa gctgaattat 3780 gttgaaactt cggcaaatgt ggaaatcgta tctaactata aagattcttt ggaaaaagag 3840 cgcgatatgg cggtgtttta tcggaacacg atagaacaca tcttggagca ttcttcgaaa 3900 gatgaccgaa tggaaagttt aaagaaggtt gttgaagaag cggagatttt tgatcagaat 3960 gcttcggtat atcacgtttt ttatgcaaat gacaagctgg ttataaaata ttttttgaag 4020 gattttgaat tcctgagcca agtggattct gtaaaacctt tgctaaaaaa acatataaag 4080 ggagacctgc attctgctgt atatctcaag ttgaaacgag aaattgaaac tggagaaatg 4140 gaagagtcgc ttagggaaat tcccgatgag agcgatcgtg cgtttatcta cattgttcta 4200 aatagcatgt ttaggtacaa ggaagatgtt tcatcgatga ttgaaaatta caagtatcag 4260 ctgacggatg aaaaacagtt gtatttcttg gtcaatcgtt tttggcataa agaaaattat 4320 gattcaaaaa aatactttgc gttttcttgg ggtggggctg taataaagtc gtttgggcga 4380 ttgtctgata aaattggatt aagtcccggt atgtaccttg gcattgactt cgtgcgtaag 4440 gattttctgt acgaatggtt tatggatatc aatggttgcc aaaataagga actggattcc 4500 ctgcagtttt atgacatgcg atgggatttt aattttggct atacattctt gaaaaaagat 4560 cacttgaact tgtttggtta tgtttctgcg gggctgggca tgaatggact tagcgctcga 4620 ggtaaagatt ctaacgacaa ggcgaataat gatttgccaa ttcagtttag tccggcgttt 4680 ggcgcgggag tgatggtgga cctgttcttt acggaaaaaa aacatattca tcatggactt 4740 cgctttagga ctggcgtgag gagccttttt tcgggggatg tcttgaaaac gtctggcgtt 4800 aggttgtatg cgtctcttga atggacgttc cgtgagtata caaagaagcc tattgatttt 4860 gactattcat tcagagaaag cggggtgaaa tgatggctcg ttggtttttg atgatgcttg 4920 gggtcatggt gtccctttct tttgcagcgc gatatactag atataatatc gttctttccg 4980 attcttctgt gggctattct caatcgcaga ccgttgctca aaaattgcaa acggctccaa 5040 ttgcttatta cggtagcctt atccaaagaa cgtatttgca gaaggatttt tataatgcgg 5100 agaatgccga attcgagcaa agatatccgc gtgtatacga gtcttttgtc gggtgggcca 5160 actataagca gaaacgccag gataatcttt tgacgcaggc tccagaacaa taccagaaga 5220 tgtttgaaca gcgctatgca ttgatgaata taaagggctt tttcttgatc cacgatgaaa 5280 tacgtgtgga aaaaatctat gacaaggcga cccatacatg ggtgactccc aaacctgaag 5340 atgcgaaaaa ggaactgttt tcttttgcgt atgtcggacc ggatatttct caagattctt 5400 cgtggataat ttcccagaga atgatggtgc ttttgcccaa ttccgaagac cagactgttc 5460 cggttaaaag aatctttttc atggatggat attcggaaga gtgctattcc ttgatggata 5520 cttcgattct tgataagatt cattacgttc cgcatcgatt gaagtatgat gtgaagccga 5580 cagctgcaat gtctgctcgt gaaaagatgc aagcctttgt agaaagcggg gattttgcat 5640 ctgcgggtgc tttggcggag tcggcggact tgtcgcctcg ttcaaaaatc cttatgaaaa 5700 ttctgaataa ggaatatgat tttattgtaa atgaagattc aacgggactc tatcttgatg 5760 attacaatgg ggtggtttac acagatcggc tggatgatgc tttaaatgat gccgctatgc 5820 atgaatatgt cagtggcagg tattgtaagt cgttgcagga taacccagat gtggattcgg 5880 ctttggtttg caaggctgtc aaaaaggttg ctttaagaga aatgccgaag cctgtagtgc 5940 attggatggg gcctattcgc tttaacgcgt atgccgattt tggacggcca ttcttggcgg 6000 gcgatttccc ggactt 6016 // |
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