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EBI DbfetchID U37523; SV 1; linear; genomic DNA; STD; PRO; 4295 BP. XX AC U37523; XX DT 10-NOV-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Sphingomonas yanoikuyae B1 isocitrate dehydrogenase (idhA) gene and idhX DE gene, complete cds, and glucosamine synthetase (glmS) gene, partial cds. XX KW . XX OS Sphingobium yanoikuyae OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; OC Sphingomonadaceae; Sphingobium. XX RN [1] RP 1-4295 RX DOI; 10.1016/0378-1119(95)00732-6 RX PUBMED; 8626059. RA Wang Y., Lau P.C.K.; RT "Sequence and expression of an isocitrate dehydrogenase-encoding gene from RT a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas yanoikuyae B1"; RL Gene 168(1):15-21(1996). XX RN [2] RP 1-4295 RA Lau P.C.K.; RT ; RL Submitted (03-OCT-1995) to the EMBL/GenBank/DDBJ databases. RL Peter C.K. Lau, Biotechnology Research Institute, Environmental RL Biotechnology, 6100 Royalmount Ave, Montreal, Quebec, H4P 2R2, Canada XX CC NCBI gi: 1052976 XX FH Key Location/Qualifiers FH FT source 1..4295 FT /organism="Sphingobium yanoikuyae" FT /strain="B1" FT /mol_type="genomic DNA" FT /note="previously Beijerinckia B1" FT /db_xref="taxon:13690" FT RBS 807..817 FT /gene="idhA" FT CDS 822..2042 FT /codon_start=1 FT /transl_table=11 FT /gene="idhA" FT /product="isocitrate dehydrogenase" FT /function="decarboxylation of isocitrate to FT alpha-ketoglutarate" FT /db_xref="GOA:P50215" FT /db_xref="HSSP:1LWD" FT /db_xref="InterPro:IPR004790" FT /db_xref="UniProtKB/Swiss-Prot:P50215" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC43640.1" FT /translation="MAKIKVKNPVVEIDGDEMTRIIWEWIRERLILPYLDVDLKYYDLS FT VEKRDETSDQITIDAANAIKEYGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNILG FT GVVFREPIVIKNVPRLVPGWTDPIVVGRHAFGDQYKATDFKVPGAGTLTMKWVGTNGEE FT LEYEVFEFPSAGVAMGMYNLDESIRDFAKASFNYGLNRGWPVYLSTKNTILKAYDGRFK FT DLFQEVFDAEFADKFKAAGIVYEHRLIDDMVASALKWSGKFVWACKNYDGDVQSDTVAQ FT GFGSLGLMTSVLLSPDGKTVEAEAAHGTVTRHYRQHQQGKATSTNPIASIFAWTQGLSF FT RGKFDDTPDVVKFAETLEQVCIKTVEGGAMTKDLALLIGPDQAWMTTEQFFEAIRVNLE FT AEMAKWA" FT stem_loop 2104..2124 FT CDS 2161..3447 FT /codon_start=1 FT /transl_table=11 FT /gene="idhX" FT /function="unknown" FT /db_xref="GOA:Q56205" FT /db_xref="InterPro:IPR000182" FT /db_xref="UniProtKB/TrEMBL:Q56205" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC43641.1" FT /translation="MTTTAKKRLEVRAAVPGDVRGIQRLIARAYPGMPNYSLATIRGQI FT NNFPNGCFVALYDSKVVGYCATMRVSKAMAFSHHDWEEITANGFGTRHSAAGEWLYGYE FT MAVDPKLRGLRIGQRLYDERKELAEELDLHGIVIAGRMPGYARAKRRAGSAADYLEKVV FT SGKLRDQVISFQLKNQFEPLGVLENYLPEDKQSDGHAAHLVWRNPYVDPDEAPEMRVPR FT GVESVRLATCQLQARAVKDFDEFVRNIEYFVDVAADYRSDFIVFPELFTLPLLSFETKK FT LSPIEAIDKLTGYTPRLTKELTRMALEYNINIIGGSHPTRADDGDIQNIAYVALRDGSV FT HTQEKIHPTPNEAFWWNIKGGDALDVIQTDCGPIGVLICYDSEFPELARRLVDQGARII FT FVPFCTDSRLGYMRVLLRRPGRSKINAMS" FT misc_feature 3731 FT /note="specific site of Tn7 insertion" FT stem_loop 3791..3816 FT CDS complement(3825..>4295) FT /codon_start=1 FT /transl_table=11 FT /gene="glmS" FT /product="glucosamine synthetase" FT /function="forms glucosamine-6-phosphate from FT fructose-6-phosphate" FT /db_xref="GOA:Q56206" FT /db_xref="HSSP:1MOQ" FT /db_xref="InterPro:IPR001347" FT /db_xref="UniProtKB/Swiss-Prot:Q56206" FT /protein_id="AAC43642.1" FT /translation="IEAMAHHIVPARDVLYLGRGTDYPLALEGALKLKEISYIHAEGYA FT AGEMKHGPIALIDELVPVICIAPSGPLFEKTVSNMQEVQARGGKVVLISAYDGVQAAGE FT GCLATITMPKVHPLIAPMVYAVPVQLLAYHVAVLKGTDVDQPRNLAKSVTVE" XX SQ Sequence 4295 BP; 874 A; 1377 C; 1307 G; 737 T; 0 other; gtcgacacgg ctgccaaagc ggatgaggcc gatgcgctgg ccggcggcga ccatgtcgcc 60 cggcttgacg aagggcacga tgcggcgcgc gacaaggccg gcaatctggg tgaagccgac 120 gcgcaggccg tcatgacgct cgaccaggat atgctggcgc tcattatcct cgctcgcctt 180 gtcgagatcg gcgttgagga acttgcccga aatataggtg acggccttca ccgtgccgcc 240 gatcggggtg cggttgatat ggacatcgaa cacgctcatg aagatcgaca cgcggatcag 300 cggctgatcg cccaggccat cgggcccagc catctcgcgc ggcggcggca cgcgctggat 360 cagggtcacc aggccatccg ccggggcgat gatcgcgccc tcatcctgcg gcacggcgcg 420 gatcggatcg cggaagaagg ccagcaccca gatggtgacc atcagcatca gccagccgat 480 gatttcccag cccatcacga agaacaggcc ggtgatcgca gccgcgatcg cgccgaactt 540 caccccttcg ggatggaccg aggggaagcg ccatttgaca ttgccgcctg cggcaatcgt 600 gatctcgtca ttttccatgg gcatgtcttt agggggagac attgatcctg acaacgacga 660 tccccgtcct tggctgccgg catgaacgcg catcgcacaa cagtcttgcg catgcatcgc 720 atgatcggcc ggaaatccgg cgctggacgg ttgaacattg gcggcgcagg ccgtagggcg 780 tccccataat tccacccaag agaagaaggc gaataggcgc tatggcgaag atcaaggtga 840 aaaaccccgt cgtggaaatc gacggcgacg aaatgacccg catcatctgg gaatggatcc 900 gtgagcgcct cattctcccc tatctcgacg tcgacctgaa atattatgac ctgtcggtcg 960 agaagcgcga cgagaccagc gaccagatca cgatcgacgc ggccaacgcg atcaaggaat 1020 atggcgtcgg cgtgaagtgc gccaccatca ccccggacga agcgcgcgtc gaggaattcg 1080 gcctcaagaa gatgtggaag tcgcccaacg gcaccatccg caacatcctg ggcggcgtcg 1140 tgttccgcga accgatcgtc atcaagaatg tcccccgcct ggttccgggc tggaccgacc 1200 cgatcgtcgt cggccgtcac gcctttggcg accagtataa ggccaccgat ttcaaggtgc 1260 ccggcgccgg cacgctgacc atgaagtggg tcggcaccaa tggcgaggaa ctcgaatatg 1320 aagtgttcga attccccagc gccggcgttg ccatgggcat gtacaatctc gacgaatcga 1380 tccgcgattt cgccaaggcc agcttcaact atggcctgaa ccgcggctgg cccgtctatc 1440 tgtcgaccaa gaacaccatc ctcaaggcct atgacggccg cttcaaggat ctgttccagg 1500 aagtgttcga cgccgaattc gccgacaagt tcaaggcggc cggcatcgtc tacgaacacc 1560 gcctgatcga cgacatggtc gcttcggcgc tgaagtggag cggcaagttc gtctgggcct 1620 gcaagaatta tgacggcgac gtccagtcgg acaccgtggc ccagggcttc ggctcgctcg 1680 gcctgatgac ctccgtcctg ctgtcgcccg acggcaagac cgtggaagcc gaagccgcgc 1740 acggcaccgt cacccgtcac tatcgccagc accagcaggg caaggcgacc tcgaccaacc 1800 cgatcgcctc gatcttcgcc tggacccagg gcctgtcgtt ccgcggcaag ttcgacgaca 1860 cgccggacgt cgtgaagttc gccgagacgc tggagcaggt ctgcatcaag accgtcgaag 1920 gcggcgcgat gaccaaggat ctggccctgc tgatcggccc ggatcaggcc tggatgacca 1980 cggagcagtt cttcgaggcg atccgcgtca atctggaagc cgaaatggcc aagtgggcct 2040 gataaaggct ttttaccgga actgatcgct tcctgaagct ttcaatacgg aaacggaacg 2100 aggcggcgcc gcatcccggc gccgtttttc cttttcggat tgatgcgacg ggagcaaaga 2160 atgaccacga cagccaagaa gcgcctggaa gtccgggcgg cggtgcccgg cgatgtgcgc 2220 ggcatccagc ggctgatcgc ccgcgcctat cccggcatgc ccaattattc gctggccacc 2280 atccgcggcc agatcaacaa tttccccaat ggctgcttcg tcgccctcta tgacagcaag 2340 gtcgtgggct attgcgccac catgcgcgtc agcaaggcga tggccttttc ccatcatgac 2400 tgggaggaga tcaccgccaa cggtttcggc acccgccaca gcgcggcggg cgaatggctt 2460 tatggctatg aaatggcggt cgatcccaag ctgcgcggcc tgcgcatcgg ccagcgcctc 2520 tatgacgagc gcaaggaact ggccgaggaa ctcgacctgc acggcatcgt cattgcgggc 2580 cgcatgcccg gctatgcccg cgccaagcgc cgcgccggca gcgcagccga ctatctggaa 2640 aaggtggtca gcggcaagct gcgcgaccag gtcatcagct tccagttgaa gaaccagttc 2700 gagccgctgg gcgtgctcga aaattacctg cccgaagaca agcagtcgga cggacatgcc 2760 gcgcatctgg tctggcgcaa cccctatgtc gacccggacg aagcgccgga aatgcgggtg 2820 ccgcgcggcg tcgaaagcgt ccgcctcgcc acctgtcagc ttcaggcgcg cgcggtgaag 2880 gatttcgacg agttcgtccg caacatcgaa tatttcgtcg atgtggcggc cgattaccga 2940 tcggacttca tcgtcttccc cgagctgttc accctgcccc tgctctcgtt cgagaccaag 3000 aaactgtcgc cgatcgaggc gatcgacaag ctgaccggct ataccccgcg cctgaccaag 3060 gaactgacgc ggatggcgct ggaatataat atcaacatca tcggcggatc gcacccgacc 3120 cgcgccgacg atggcgacat ccagaatatc gcctatgtcg cgctgcgcga cggatcggtc 3180 cacacccagg aaaagatcca cccgaccccg aacgaggcct tctggtggaa catcaagggc 3240 ggcgacgcgc tcgacgtgat ccagaccgac tgcgggccga tcggggtact gatctgctat 3300 gacagcgaat ttcccgaatt ggcccgccgc ctggtcgatc agggcgcgcg catcatcttc 3360 gttcccttct gcaccgattc gcgcctgggc tatatgcgcg tgctattgcg caggcccggg 3420 cgatcgaaaa tcaatgctat gtcgtgatgt cgggcaatgt cggcaatctg cccaatgtcg 3480 acaatatgga cattcaatat gcccagagcg cgatcctgac gccctgcgac ctgcccttcg 3540 cgcgggacgg catcgcggcc gaggcaagcg agaatgtcga gacgctgacc atggcggacg 3600 tgaacctggc cgacctcagc tgggcacgcg ccgaacggac ggttcgcaac ctgcgcgacc 3660 gccgcttcga cctctatcat atcgactggg acagcaatcc cgaccacagc cacgcaccaa 3720 gcggcggccc ggtgccggcc ggtggccata attcaccggg tggtggctga tcgaagcgcg 3780 agcagccaaa gcccctgcca gcatgacggc aggggcagtc cggctcactc gacggtgaca 3840 ctcttcgcca gattgcgcgg ctgatcgacg tcggtgccct tgagcacggc aacatgatag 3900 gcgagcagct gaaccggcac ggcatagacc atcggcgcga tcagcgggtg gaccttgggc 3960 atggtgatgg tggcaaggca gccttcgccc gccgcctgca ccccgtcata tgcggaaatc 4020 agcaccacct tgccgccgcg cgcctgcact tcctgcatgt tgctgacggt cttttcgaac 4080 aaggggccgc tgggcgcgat gcagatcacc ggcaccagtt cgtcgatcag cgcgatcggg 4140 ccatgcttca tctcgccggc ggcatagccc tcggcatgga tatagctgat ttccttgagt 4200 ttcagcgcac cttccagcgc cagcggatag tcggtgccgc ggccgagata caggacatcg 4260 cgggccggca cgatatgatg ggccatcgcc tcgat 4295 // |
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