EBI Dbfetch

ID   U33817; SV 1; linear; mRNA; STD; PLN; 1983 BP.
XX
AC   U33817;
XX
DT   23-DEC-1995 (Rel. 46, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 18)
XX
DE   Sorghum bicolor dhurrinase mRNA, nuclear gene encoding chloroplast protein,
DE   complete cds.
XX
KW   .
XX
OS   Sorghum bicolor (sorghum)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; PACCAD clade;
OC   Panicoideae; Andropogoneae; Sorghum.
XX
RN   [1]
RP   1-1983
RA   Cicek M., Esen A.;
RT   "Cloning and Sequencing of a cDNA Coding for Beta-Glucosidase (Dhurrinase)
RT   from Sorghum bicolor (L) Moench 1 (Accession No. U33817) (PGR95-097)";
RL   Plant Physiol. 109(4):1497-1497(1995).
XX
RN   [2]
RP   1-1983
RA   Cicek M., Esen A.;
RT   ;
RL   Submitted (13-AUG-1995) to the EMBL/GenBank/DDBJ databases.
RL   Asim Esen, Biology, Virginia Polytechnic Institute and State University,
RL   Blacksburg, VA 24061-0406, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1983
FT                   /organism="Sorghum bicolor"
FT                   /strain="P721N"
FT                   /mol_type="mRNA"
FT                   /dev_stage="3-4 day-old"
FT                   /tissue_type="etiolated shoots"
FT                   /note="5' and 3' ends were cloned into PCR script SK(+)
FT                   vector and sequenced completely"
FT                   /db_xref="taxon:4558"
FT   CDS             71..1768
FT                   /codon_start=1
FT                   /product="dhurrinase"
FT                   /function="beta-glucosidase, catalyzes the hydrolysis of
FT                   the cyanogenic beta-glucoside dhurrin"
FT                   /db_xref="GOA:Q41290"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="PDB:1V02"
FT                   /db_xref="UniProtKB/TrEMBL:Q41290"
FT                   /protein_id="AAC49177.1"
FT                   /translation="MALLLASAINHTAHPAGLRSHPNNESFSRHHLCSSPQNISKRRSN
FT                   LSFRPRAQTISSESAGIHRLSPWEIPRRDWFPPSFLFGAATSAYQIEGAWNEDGKGPST
FT                   WDHFCHNFPEWIVDRSNGDVAADSYHMYAEDVRLLKEMGMDAYRFSISWPRILPKGTLA
FT                   GGINEKGVEYYNKLIDLLLENGIEPYITIFHWDTPQALVDAYGGFLDEEDYKDYTDFAK
FT                   VCFEKFGKTVKNWLTFNEPETFCSVSYGTGVLAPGRCSPGVSCAVPTGNSLSEPYIVAH
FT                   NLLRAHAETVDIYNKYHKGADGRIGLALNVFGRVPYTNTFLDQQAQERSMDKCLGWFLE
FT                   PVVRGDYPFSMRVSARDRVPYFKEKEQEKLVGSYDMIGINYYTSTFSKHIDLSPNNSPV
FT                   LNTDDAYASQETKGPDGNAIGPPTGNAWINMYPKGLHDILMTMKNKYGNPPMYITENGM
FT                   GDIDKGDLPKPVALEDHTRLDYIQRHLSVLKQSIDLGADVRGYFAWSLLDNFEWSSGYT
FT                   ERFGIVYVDRENGCERTMKRSARWLQEFNGAAKKVENNKILTPAGQLN"
XX
SQ   Sequence 1983 BP; 535 A; 477 C; 496 G; 474 T; 1 other;
     tgaattcgtg ggcaactcac cactcaagca gctcaaacct ttagccacta cacagcagct        60
     atagcaagaa atggctctac ttcttgcttc tgccataaat cacactgccc atccagcagg       120
     ccttagaagc catcccaata atgagagttt ctcacggcac cacctatgtt cttcaccaca       180
     aaacatcagt aagcgaaggt ctaaccttag ctttaggcca cgagctcaaa cgataagcag       240
     tgagtctgct ggaatccaca ggctgagccc ctgggaaatc cctaggaggg actggttccc       300
     tcctagcttt ctcttcggtg ccgccacttc agcataccaa attgaaggtg cttggaatga       360
     agatgggaag gggccaagca catgggatca cttctgccac aattttccgg aatggatagt       420
     ggacaggagc aatggggatg ttgcagcgga ttcgtaccat atgtacgctg aggatgtcag       480
     gttgctgaag gaaatgggca tggacgccta taggttctcc atctcttggc ccagaatact       540
     gccgaagggg acgctcgctg gaggtattaa cgagaaaggt gtcgagtact acaacaagtt       600
     gatcgacttg ttgttagaga acggcataga gccatatata acaattttcc actgggacac       660
     gcctcaagcg ctggtagacg cgtatggcgg cttcttagat gaggaggatt ataaagatta       720
     cacagacttc gctaaagtgt gctttgaaaa gtttggtaag acggtgaaaa actggttgac       780
     ctttaatgag ccggagacat tctgttctgt ttcctatgga actggagtcc ttgccccggg       840
     gcggtgctca ccgggagtaa gttgtgccgt tccaactgga aactcactca gcgagccata       900
     cattgttgcc cacaaccttc tccgagctca tgctgagact gttgatattt acaacaagta       960
     tcacaagggt gcggacggcc gtatagggct tgcgttaaat gtatttggcc gtgtgccata      1020
     cacaaatacg tttcttgatc aacaggccca agaaaggtcc atggacaaat gcctaggatg      1080
     gttcttggag ccagtggttc gtggtgacta ccccttctcc atgagagtgt cagcgaggga      1140
     ccgggtgccc tacttcaaag aaaaagagca agagaagcta gttggttctt acgatatgat      1200
     cgggataaac tactacacct caacgttctc caagcacatc gacctctcac caaacaactc      1260
     gccagtgctc aacactgacg acgcctatgc cagtcaagaa accaaagggc ctgacgggaa      1320
     tgctattggt cctcctactg gaaatgcgtg gatcaatatg taccctaaag gcctacacga      1380
     tatccttatg accatgaaga acaagtatgg aaacccacca atgtacatca ctgagaacgg      1440
     gatgggggac attgacaagg gcgatctacc caaaccagtt gcgttggaag accacacaag      1500
     gctagattac atccagcgcc acctctcagt tcttaaacaa tcaatagact tgggagcaga      1560
     cgtgcgcggc tacttcgcgt ggtctctgct ggacaacttc gaatggtcca gcggctacac      1620
     cgagcgtttc ggcatcgtct acgtcgatcg cgagaatggc tgcgagcgca ccatgaagcg      1680
     atcagccagg tggttgcagg agttcaatgg agctgccaag aaggttgaaa ataacaagat      1740
     tcttacgccg gccggccagc ttaactaatt aagtagaaca cgtactgctt ctggctgatg      1800
     tatggtatgg tggcactgtg tcttctgtgc atgcctgtat ggtctgaaac gacatatttt      1860
     actttgcacg gctagaggta gtggaggcat attctccagc gccgcagcta ataatgcaat      1920
     gctctgttcc agttcagtgt tatcttttta ttatgcaata aaacgaagtg ntcgatttgc      1980
     aat                                                                    1983
//