EBI Dbfetch

ID   U32615; SV 1; linear; genomic DNA; STD; FUN; 1409 BP.
XX
AC   U32615;
XX
DT   30-AUG-1995 (Rel. 45, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Schizosaccharomyces pombe diadenosine 5',5'''-P1,P4-tetraphosphate
DE   asymmetrical hydrolase (aph1) gene, complete cds.
XX
KW   Ap4A hydrolase; aph1 gene; bis(5'-nucleosidyl) oligophosphate;
KW   diadenosine tetraphosphate; dinucleoside tetraphosphate; histidine triad.
XX
OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
XX
RN   [1]
RP   1-1409
RX   PUBMED; 8554540.
RA   Huang Y., Garrison P.N., Barnes L.D.;
RT   "Cloning of the Schizosaccharomyces pombe gene encoding diadenosine
RT   5',5''-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: sequence
RT   similarity with the histidine triad (HIT) protein family";
RL   Biochem. J. 312(Pt 3):925-932(1995).
XX
RN   [2]
RP   1-1409
RA   Garrison P.N.;
RT   ;
RL   Submitted (28-JUL-1995) to the EMBL/GenBank/DDBJ databases.
RL   by Preston N. Garrison, Dept. of Biochemistry, Univ. of Texas Health
RL   Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX
RL   78284-7760, USA
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FH   Key             Location/Qualifiers
FH
FT   source          1..1409
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="972h-"
FT                   /mol_type="genomic DNA"
FT                   /note="from genomic library in pFL20"
FT                   /db_xref="taxon:4896"
FT   CDS             join(266..328,457..558,623..709,819..1115)
FT                   /codon_start=1
FT                   /gene="aph1"
FT                   /product="diadenosine 5',5'''-P1,P4-tetraphosphate
FT                   asymmetrical hydrolase"
FT                   /EC_number="3.6.1.17"
FT                   /note="Ap4A hydrolase;  bis(5'-nucleosidyl) oligophosphate
FT                   hydrolase"
FT                   /db_xref="GENEDB:SPCC4G3.02"
FT                   /db_xref="GOA:P49776"
FT                   /db_xref="InterPro:IPR001310"
FT                   /db_xref="InterPro:IPR011146"
FT                   /db_xref="InterPro:IPR011151"
FT                   /db_xref="InterPro:IPR019808"
FT                   /db_xref="UniProtKB/Swiss-Prot:P49776"
FT                   /protein_id="AAC49143.1"
FT                   /translation="MPKQLYFSKFPVGSQVFYRTKLSAAFVNLKPILPGHVLVIPQRAV
FT                   PRLKDLTPSELTDLFTSVRKVQQVIEKVFSASASNIGIQDGVDAGQTVPHVHVHIIPRK
FT                   KADFSENDLVYSELEKNEGNLASLYLTGNERYAGDERPPTSMRQAIPKDEDRKPRTLEE
FT                   MEKEAQWLKGYFSEEQEKE"
FT   exon            <266..328
FT                   /gene="aph1"
FT   intron          329..456
FT                   /gene="aph1"
FT                   /note="confirmed by cDNA sequence"
FT   exon            457..558
FT                   /gene="aph1"
FT   intron          559..622
FT                   /gene="aph1"
FT                   /note="confirmed by cDNA sequence"
FT   exon            623..709
FT                   /gene="aph1"
FT   intron          710..818
FT                   /gene="aph1"
FT                   /note="confirmed by cDNA sequence"
FT   exon            819..>1115
FT                   /gene="aph1"
FT   misc_feature    849..863
FT                   /gene="aph1"
FT                   /note="encodes histidine triad (HVHVH)"
XX
SQ   Sequence 1409 BP; 422 A; 259 C; 257 G; 471 T; 0 other;
     gaaacttgtg tgcatacgaa taataaaatt cgataatatt ggaattttta gtccgcttta        60
     tctgttccat gatactgtta cttacatata tgcaagacgc tattttctca tagtctgttt       120
     gttttttaag tatatcaatc tttcttatta tattccatag acactttcgc acatgactct       180
     ccagggactc cgcgatatgg gttgtgagca tcgtgaagct gaattcaacc aacaacttag       240
     attcttacaa tattcgtaag ccagaatgcc aaaacagcta tatttctcca agtttcctgt       300
     tggaagtcaa gttttttatc gtactaaggt aagttaacgg tctcatgtgt gtagatattg       360
     gtgtttgcaa acttttgttt gtcattctta tttattctat aacggcagac agtttgtgat       420
     ttttctttgg ttgaggtcag ctgctaacga ttttagttat ctgccgcgtt tgtaaacctg       480
     aaaccaattt taccaggtca tgttttggta attccgcaac gggcggtccc tagattgaaa       540
     gatttgacac cttcagaggt aggattctta tgctattcga aaaaataatg gaatctgcat       600
     acgctaacta atgaaaactt agttgacgga tttgtttact tctgttcgca aagtgcaaca       660
     ggtaatcgaa aaggtgtttt cggcatctgc atcaaacatt ggtattcaag taagtacttt       720
     gatagtcaag gaataaataa aaaaacatat tccttttcac attcaaaata aaaaatcgtt       780
     ttaatttaga agctgacatt ttgcttttaa ctcaatagga tggtgtagac gctggtcaaa       840
     cagttcctca tgtacatgtt cacattatcc ctcgtaaaaa ggcagatttt tcagaaaacg       900
     atctagtcta cagtgagttg gaaaaaaacg aaggaaatct tgcttccctt tatcttacgg       960
     gaaatgagcg gtatgcagga gatgagagac cgccaaccag tatgaggcaa gctattccta      1020
     aggacgagga tcgtaagcca agaacacttg aggaaatgga aaaggaagct cagtggttga      1080
     aagggtactt ttccgaagag caagagaagg aataaaaagt tgaagtacct caataccaca      1140
     ggggtagtgt ttacgtatga attaagctaa atattatatg accctttttt tttatttcac      1200
     ccaaggttac aagaaaaatt tccttttttc tctctaccct gcttacattg catctgtctg      1260
     ctgagcttta gcaacacaac gtaaccatac atattgtgat gaacccttct acaattcgat      1320
     cgaattagct tcagttccct attttgattt tgctctcttt ctttcatcct ttcctcataa      1380
     ccctactaga tatccatctt tttgaattc                                        1409
//