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EBI DbfetchID U32379; SV 1; circular; genomic DNA; STD; PRO; 5807 BP. XX AC U32379; L26258; XX DT 12-NOV-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 11) XX DE Bacillus subtilis plasmid pTA1015, complete sequence. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OG Plasmid pTA1015 XX RN [1] RP 1-5807 RA Hara T., Yasuda S., Ogata S., Soeda E., Ueda S.; RT "Complete nucleotide sequence of Bacillus subtilis (natto) plasmid RT responsible for gamma-polyglutamate synthesis"; RL J. Fac. Agric. Kyushu Univ. 36:209-218(1992). XX RN [2] RX DOI; 10.1093/hmg/3.6.853 RX PUBMED; 7951227. RA Tomlinson I.M., Cook G.P., Carter N.P., Elaswarapu R., Smith S., Wlater G., RA Buluwela L., Rabbitts T.H., Winter G.; RT "Human immunoglobulin VH and D segments on chromosomes 15q11.2 and RT 16p11.2"; RL Hum. Mol. Genet. 3(6):853-860(1994). XX RN [3] RX DOI; 10.1093/nar/23.4.612 RX PUBMED; 7899081. RA Meijer W.J., Venema G., Bron S.; RT "Characterization of single strand origins of cryptic rolling-circle RT plasmids from Bacillus subtilis"; RL Nucleic Acids Res. 23(4):612-619(1995). XX RN [4] RP 1-5807 RX DOI; 10.1016/S0168-6445(98)00003-5 RX PUBMED; 9532747. RA Meijer W.J., Wisman G.B., Terpstra P., Thorsted P.B., Thomas C.M., RA Holsappel S., Venema G., Bron S.; RT "Rolling-circle plasmids from Bacillus subtilis: complete nucleotide RT sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, RT and comparisons with related plasmids from gram-positive bacteria"; RL FEMS Microbiol. Rev. 21(4):337-368(1998). XX RN [5] RP 1-5807 RA Meijer W.J.J.; RT ; RL Submitted (01-FEB-1994) to the EMBL/GenBank/DDBJ databases. RL Department of Genetics, University of Groningen, Kerklaan 30, Haren, RL Groningen 9751 NN, The Netherlands XX RN [6] RP 1-5807 RA Meijer W.J.J.; RT ; RL Submitted (08-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL Department of Genetics, University of Groningen, Kerklaan 30, Haren, RL Groningen 9751 NN, The Netherlands XX RN [7] RP 1-5807 RA Meijer W.J.J.; RT ; RL Submitted (26-JUL-1995) to the EMBL/GenBank/DDBJ databases. RL Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, RL Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands XX CC On Sep 10, 2003 this sequence version replaced gi:529115. XX FH Key Location/Qualifiers FH FT source 1..5807 FT /organism="Bacillus subtilis" FT /plasmid="pTA1015" FT /strain="IAM1028" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT gene 166..1185 FT /gene="rep" FT CDS 166..1185 FT /codon_start=1 FT /transl_table=11 FT /gene="rep" FT /product="replication protein" FT /note="Rep; involved in initiation of replication" FT /db_xref="GOA:Q45447" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q45447" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44413.1" FT /translation="MYSSENDYIILEDKTATGKKRDWKGKKRRTNLMAEHYEALQSKTG FT IPYYGKKAEKLCSCAECLSFKRDPETGKLKLYQAQFCKVRLCPMCAWRRSLKIAYHNKL FT IVEEANRQYGCGWIFLTLTVRNVEGDGLKPMIADMMKGWNRLFGYKRVKVATLGYFRAL FT EITKNHEEDTYHPHFHVLLPVKKSYFTHNYIKQSEWTSLWKRAMKLDYTPIVDIRRVKG FT RAKIDAEQIESDVREAMMEQKAVLEISKYPVKDTDVVRGNKVTEDNLNTVFYLDDALSA FT RRLIGYGGILKEIHKELNLGDAEDGDLVKIEEEDDEVANEAFEVMAYWHPGIKNYIIR" FT CDS 1401..1841 FT /codon_start=1 FT /transl_table=11 FT /product="putative exported protein" FT /note="orf1" FT /db_xref="UniProtKB/TrEMBL:Q57219" FT /protein_id="AAC44414.1" FT /translation="MVTTIGKSKMWVGIIVVLSLLLVSFSPAVKADTKDKYYSTTSTQS FT STKSYRKANTSGVYVKVLKAGRSRDVAISVFADANKGKGKPHWVNVSGSDGATLGKYVT FT AGHTYHLTNYAVERYGKNVPIQLFVSNGSGKKVEFYWSPDCR" FT repeat_region 1860..1885 FT /rpt_type=INVERTED FT gene 1932..2505 FT /gene="sipP" FT RBS 1932..1936 FT /gene="sipP" FT CDS 1945..2505 FT /codon_start=1 FT /transl_table=11 FT /gene="sipP" FT /product="type I signal peptidase" FT /note="SipP; involved in processing of pre-proteins" FT /db_xref="GOA:P37943" FT /db_xref="InterPro:IPR000223" FT /db_xref="InterPro:IPR011056" FT /db_xref="InterPro:IPR015927" FT /db_xref="InterPro:IPR019756" FT /db_xref="InterPro:IPR019757" FT /db_xref="InterPro:IPR019758" FT /db_xref="InterPro:IPR019759" FT /db_xref="UniProtKB/Swiss-Prot:P37943" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44415.1" FT /translation="MTKEKVFKKKSSILEWGKAIVIAVILALLIRNFLFEPYVVEGKSM FT DPTLVDSERLFVNKTVKYTGNFKRGDIIILNGKEKSTHYVKRLIGLPGDTVEMKNDHLF FT INGNEVKEPYLSYNKENAKKVGINLTGDFGPIKVPKDKYFVMGDNRQESMDSRNGLGLF FT TKDDIQGTEEFVFFPFSNMRKAK" FT repeat_region 2517..2538 FT gene 2840..4288 FT /gene="mob15" FT CDS 2840..4288 FT /codon_start=1 FT /transl_table=11 FT /gene="mob15" FT /product="mobilization protein" FT /note="Mob15; essential for conjugative plasmid FT mobilization" FT /db_xref="GOA:Q45448" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/TrEMBL:Q45448" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44416.1" FT /translation="MANYAVIRMEKYKKDRLNGTQKHNQREFQKSKNENIDRERTHLNY FT DLVNEKPISYSKAIHEKIEGRVKRKVRADAVLVSEFLITASPDYMNGLSDEEQRRYFET FT AVDHLKEKYSAENMLYATVHMDEATPHMHVGIVPITEDGRLSAKDFFNGKLKMKAIQDD FT FHRHMVENGFDLVRGEPSEKKHENVHQYKINQRQAELERLNAEIALKEKQREELEKQNK FT AVQAVIEVKKESLTAKAEELKMPTIEHEKAWLKKDKVIVPERELHALYAYAEQKTKTAA FT ELAGQLKSETQEKERWQSIARQEADRADEKDQRLQELQSRIHSEVEASKKEMRRKLAKE FT FTEEQRQDLRQEVKEELTTLRTENEELSAENKVLIIQRNSEAAESLKLKQELDKRNGQY FT AEVLSFAKKQNQTLEKVAGENKALKKENKTLKERVAVLDQWKDKMVQWAKEKLPKMRKL FT AASFFRTAGMPREANKYKDNELER" FT CDS complement(4299..4778) FT /codon_start=1 FT /transl_table=11 FT /product="putative DNA-binding protein" FT /note="orf2C15; contains helix-turn-helix motif" FT /db_xref="GOA:Q45449" FT /db_xref="InterPro:IPR009061" FT /db_xref="UniProtKB/TrEMBL:Q45449" FT /protein_id="AAC44417.1" FT /translation="MDMGIKLSSKDVAKHLGIEPVTVRKYASMLEEQGYSFNKDSKGWR FT LYTEDDVKTLEYLCTMTKINGHSLDETVKHIASLYRSKLTISDTATSLQDNPLVEFMKR FT QEEFNRHMAERMERFEKRQQERHETLTAALRESLETQKMIAATKQKKWWKFWKGN" FT rep_origin 5148..5368 FT /note="palT1 SSO; single strand origin; ssDNA conversion FT signal" XX SQ Sequence 5807 BP; 1807 A; 958 C; 1405 G; 1637 T; 0 other; ttgatactat atagaaacat ctcaaggcga aaaaatagcc cccatccctt atttgtcaag 60 ggtttgacgg ctttttgaca tgtagaaact ccttccgcta ttattaaagt gcccactaaa 120 ataatagaat gctagattac tagctcagaa ggagtttttt tgttcatgta ttcatctgaa 180 aatgattata tcatccttga ggacaagacc gcaacaggta aaaagcggga ttggaagggg 240 aaaaagagac ggacgaatct tatggctgag cactatgaag ctttacagag taaaactggt 300 ataccttact atggcaaaaa agctgagaaa ttgtgcagtt gtgcggaatg tctttcgttt 360 aaacgagacc cggagacggg caaattaaag ttgtatcaag ctcagttttg taaagtgagg 420 ttatgcccga tgtgtgcgtg gcgtaggtct ttaaaaattg cttatcataa taaattaatc 480 gttgaggaag cgaatcggca gtacggttgt ggatggattt ttctcacact gacggttcgg 540 aatgtcgagg gtgacggatt aaaacccatg attgctgaca tgatgaaagg atggaaccgc 600 cttttcggat ataaacgagt taaggtagcg actttaggtt atttcagagc tttagagatt 660 accaaaaatc acgaagaaga tacatatcat ccgcattttc atgtgttgtt gcctgtgaag 720 aaaagctatt ttactcacaa ttacattaag cagtctgagt ggacgagctt atggaaaagg 780 gcgatgaaac tggactacac gccgattgtt gatatccgaa gagtcaaggg aagagctaaa 840 attgatgccg aacagattga gagcgatgtg cgggaagcca tgatggagca aaaagctgtt 900 cttgaaatct ctaaatatcc ggttaaagat acggatgttg tgcgcggcaa taaggtgaca 960 gaagacaatc tgaacacggt gttttatttg gatgatgcgc tttctgcccg ccggcttatt 1020 ggttacggtg gcatcttgaa ggaaattcat aaagaactaa acctcggtga tgcggaggac 1080 ggcgatctcg tcaagattga ggaagaagat gacgaggtgg cgaacgaagc atttgaagtt 1140 atggcttact ggcatccagg cattaaaaat tacataatca gataaaaagc aggcgttgtt 1200 cctgcttttt ttatactcta atagtcaaat caagagttaa ttttagatgt aattgtgaga 1260 attagagtgg ctgaccagta tttgaaactt cttgggctac tttcttaact ttatattaaa 1320 actatgtata tatgtgttgt tttttctatt attttgatat tatttacaag tattgaattt 1380 tgctaggagg gaaagttttt atggttacca cgattggtaa aagtaagatg tgggtaggta 1440 ttattgttgt attatcttta ttattggtat ctttttcgcc tgctgtaaag gctgatacta 1500 aggataaata ttattctaca acttctaccc aatcttcaac aaaaagttat cgtaaagcta 1560 atactagtgg ggtctatgtg aaagtactta aagctggacg ttctcgtgat gttgctattt 1620 cagtttttgc tgatgcgaac aaagggaaag gaaagccaca ttgggtaaat gtttctggta 1680 gcgatggcgc tactctggga aaatacgtga ctgcagggca tacatatcat cttacaaact 1740 atgctgtaga acgttatgga aagaatgttc ctatacaatt atttgtttct aatggttctg 1800 gcaaaaaagt tgaattttat tggagtcccg attgtagata gtccaaatag cagatgatga 1860 aaaagcagga ttaatcctgc ttttttattt ttgttttggt aaaatgtgat aagcgggttt 1920 tgaaatatag aggaggaaat ttctttgaca aaagagaaag ttttcaaaaa gaaaagctct 1980 attttagagt ggggcaaggc tattgtgata gctgttatac ttgctcttct tattcggaat 2040 tttttgtttg agccatatgt ggtagaaggg aagtctatgg atcccacttt ggttgattct 2100 gaaagattat ttgtaaataa gactgtgaag tatacaggta attttaaacg aggggatata 2160 ataattttaa acggaaagga aaaaagcaca cattatgtga agcgattaat tggtttacct 2220 ggagacactg tagaaatgaa gaatgaccac ctttttatta atggaaatga agttaaggaa 2280 ccatatcttt cttataataa agaaaatgct aagaaagtgg gtataaacct tacaggagat 2340 tttggaccaa ttaaagttcc aaaagataaa tattttgtta tgggcgataa ccgacaagaa 2400 tcaatggata gtcgtaatgg gcttggactc tttactaaag atgatattca gggaaccgaa 2460 gagttcgtat tttttccatt tagtaatatg cgaaaagcta aataattttt agaccgaaac 2520 aggctataag gtctgttttt tcattttgtt atgaacattt agcacacaga tcaaagtttt 2580 catagtttga atgctttgat agcagcaaag ggtatttctg attttctgcc gatctctcat 2640 cggcggaaaa gtcgggtcgg cggacagccg acaagtggca cgaactttcg atgcgacagc 2700 gagaatgaga gagccaccag caccgcaggt cgcacgtcca aatttgccat gggcataatt 2760 tggtgtagtg cgttacacca aagataaact ttgtgttacc ataaccccta tacagtggtc 2820 tgaatcgggg gtttttctca tggcaaatta tgcagtcatc aggatggaaa aatacaaaaa 2880 agatagattg aatggaacgc aaaaacacaa tcagcgggag tttcaaaaaa gcaaaaatga 2940 aaatattgat cgggagcgga cgcacttaaa ttatgatcta gtgaacgaga aaccgattag 3000 ctattcaaaa gcgattcatg aaaaaattga ggggcgagtc aaacggaagg tccgagcgga 3060 tgctgttttg gtcagcgaat ttttgatcac ggcaagtcct gactatatga atgggctgag 3120 cgatgaggag cagcggcgct attttgaaac agcggttgat catttgaaag agaaatacag 3180 cgctgaaaac atgctttatg ctacagtcca tatggatgaa gcgactcctc atatgcatgt 3240 tggtattgta ccgatcacag aggacggccg actctctgcg aaagattttt ttaatggcaa 3300 attgaagatg aaagccattc aagatgattt tcatcggcac atggttgaaa acggttttga 3360 cctggtgcgc ggcgaaccaa gcgaaaagaa gcatgagaat gttcaccagt ataaaataaa 3420 tcagcggcaa gcggagcttg agcggcttaa tgctgaaatt gctttaaagg aaaagcagag 3480 agaggaactg gaaaagcaaa acaaagctgt tcaagcagtt atagaagtga aaaaagaatc 3540 gctgacagct aaggctgaag agttgaaaat gccgactatt gaacatgaaa aagcgtggct 3600 caaaaaggat aaagtcattg tgccagagcg ggaactccat gctttgtatg cctatgcgga 3660 gcagaaaact aaaacggcag ccgagttggc ggggcaattg aagtcggaaa cgcaggaaaa 3720 ggagcgctgg cagtctatcg cccggcagga agcagatcgg gcggatgaaa aagaccaacg 3780 gcttcaggaa ctgcagagta ggatccattc agaagttgaa gcgtccaaaa aggaaatgcg 3840 gcgcaagctt gcaaaggaat ttacggaaga acagcgtcag gatcttcggc aggaagtgaa 3900 agaggaactg acgactttac gaacggaaaa cgaggaactg tcagctgaaa ataaagtttt 3960 gatcattcaa agaaatagcg aagctgcgga gagcctaaaa ctaaaacagg aacttgataa 4020 gagaaacggg cagtatgctg aggttttgag tttcgccaag aagcagaatc aaacgcttga 4080 aaaagtggct ggagaaaaca aggcgttaaa aaaagaaaat aagacactaa aagagagagt 4140 tgccgtactg gaccaatgga aagacaaaat ggttcagtgg gctaaagaaa aattaccaaa 4200 gatgcggaaa ttagcggcat cgtttttccg tacggctgga atgcctagag aagccaataa 4260 atacaaggac aatgaattag agcggtgaaa acgggcaatc aattgccctt ccaaaatttc 4320 caccactttt tttgtttggt ggctgcgatc attttttgtg tctcaaggga ctcacgtaat 4380 gcagcggtga gtgtctcgtg cctttcttgc tgtctttttt cgaatcgttc catccgttct 4440 gccatgtggc ggttgaattc ttcctgtcgt ttcatgaatt cgactaaagg attgtcctgt 4500 agcgatgtag cggtatccga tatggtcagt ttagaacgat ataagcttgc tatgtgcttt 4560 accgtttcgt cgagtgagtg tccattgatc ttagtcattg tacatagata ctctaaagtc 4620 tttacgtcat cctcggtata gagtcgccat cctttcgaat ctttattgaa cgagtagcct 4680 tgttcttcaa gcatactggc atacttgcgg acagttactg gctctatacc gaggtgtttt 4740 gcgacgtcct ttgatgataa tttgattccc atatccataa cgtatcacct cgtaaaaagg 4800 gttcgctata gtgagggcaa agcctttatt tagagccatt taaaaagaaa gctgttccgt 4860 tcttttatca ggttgggact gtatttagtt tatgggggag tgccaatgag tgattttgaa 4920 acaggaatga gatatgtaag agcaaccctt ggctttgagg gtttagtgct gacggaagaa 4980 gaggaaaagc ttttagaaag acggtttcat ggagaaatca cagaggaaga atacatacaa 5040 aaagcgttga gcttcttgat gtgatgtacc tgcagggctt ataatattca tagctgttgt 5100 tagaacctaa acccttttac cgtttttggc ggtgagggtt cttttttttt aggcagtgat 5160 gcgattttgg cgtgagtcaa cggtaaccgg accgtaggga ggattaagga gttgactcgc 5220 tcagcgccac ccgaaccctt tcagcactca aacaaacccg tttgtttgac gccaaccggc 5280 agggagcccc ccgaagaagc gggggttggg gggattgaat gctggcatcc aacggccgtc 5340 cgttggtggg tttgggcaaa gccaagaact gttgcaaggc tcgttgagaa taaagaatgc 5400 ttttcaggat gcttagaatc gtttctgaga gcttcaaata aaaaagatga ccttttatag 5460 ggggaagctc ttaaaattga atgtaggggc atttaaacac gtttaaaaat aaaaaaagca 5520 gactctttag agtccgcctt gttattttta acccagtgct ccattttcgg ctgtttggaa 5580 atcttttgag atgccgaacc atccattttc ttttgttcca tgaaaaaagt gcttttggat 5640 gcttaaaaag gctttttcgt ataaaaaaag cccgattttt gaaaaaaaaa tctccccccc 5700 tgcggaagaa tggttttgat ctttgggttt taggttttaa aaaaagccgg ctgttttcag 5760 ccggtttttt tcgattttgg cggacggaaa tcgggtcttt tcttatc 5807 // |
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