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EBI DbfetchID U32378; SV 1; circular; genomic DNA; STD; PRO; 7837 BP. XX AC U32378; Z36269; Z46671; XX DT 08-NOV-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 12) XX DE Bacillus subtilis plasmid pTA1040, complete sequence. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OG Plasmid pTA1040 XX RN [1] RP 1-7837 RX DOI; 10.1093/nar/23.4.612 RX PUBMED; 7899081. RA Meijer W.J., Venema G., Bron S.; RT "Characterization of single strand origins of cryptic rolling-circle RT plasmids from Bacillus subtilis"; RL Nucleic Acids Res. 23(4):612-619(1995). XX RN [2] RP 1-7837 RX DOI; 10.1111/j.1365-2958.1995.mmi_17040621.x RX PUBMED; 8801417. RA Meijer W.J., de Jong A., Bea G., Wisman A., Tjalsma H., Venema G., Bron S., RA van Dijl J.M.; RT "The endogenous Bacillus subtilis (natto) plasmids pTA1015 and pTA1040 RT contain signal peptidase-encoding genes: identification of a new structural RT module on cryptic plasmids"; RL Mol. Microbiol. 17(4):621-631(1995). XX RN [3] RP 1-7837 RX DOI; 10.1016/S0168-6445(98)00003-5 RX PUBMED; 9532747. RA Meijer W.J., Wisman G.B., Terpstra P., Thorsted P.B., Thomas C.M., RA Holsappel S., Venema G., Bron S.; RT "Rolling-circle plasmids from Bacillus subtilis: complete nucleotide RT sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, RT and comparisons with related plasmids from gram-positive bacteria"; RL FEMS Microbiol. Rev. 21(4):337-368(1998). XX RN [4] RP 1-7837 RA Meijer W.J.J.; RT ; RL Submitted (08-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, RL Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands XX RN [5] RP 1-7837 RA Meijer W.J.J.; RT ; RL Submitted (17-NOV-1994) to the EMBL/GenBank/DDBJ databases. RL Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, RL Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands XX RN [6] RP 1-7837 RA Meijer W.J.J.; RT ; RL Submitted (26-JUL-1995) to the EMBL/GenBank/DDBJ databases. RL Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, RL Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands XX CC On or before Sep 10, 2003 this sequence version replaced gi:624247, CC gi:530272. XX FH Key Location/Qualifiers FH FT source 1..7837 FT /organism="Bacillus subtilis" FT /plasmid="pTA1040" FT /strain="IAM1232" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT gene 165..1184 FT /gene="rep40" FT CDS 165..1184 FT /codon_start=1 FT /transl_table=11 FT /gene="rep40" FT /product="Rep protein" FT /note="involved in initiation of replication" FT /db_xref="GOA:Q45441" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q45441" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44406.1" FT /translation="MYSSESNYSIFQDKTATGKKRDWKGKKVRSLLMAAHYEGLEKRTG FT APYYGKKAEKVCDCAECLAFKRDLETGRLRLYQAYFCKVRLCPMCAWRRSLKIAYHNKL FT IVEEANRQYKPAWIFLTLTVKNVEGDDLKQTISDMMKGFNKLMKYKKVDSAVRGYFRAL FT EITKNHEENTYHPHFHVLIPVRTGYFGKNYINQKEWTSFWKRAMKWDYTPIVHVQRVKG FT KKGIDAEAIEQEVRKEMEEQKAILEISKYPVKDTDVIRGNEVTEENLDTVYYLDDALSA FT RRLIGYGGILKEIHKELNLTDAEDGDLIRIEEDDDDVANETFEVMAHWHVGIKNYIIT" FT gene complement(1224..1829) FT /gene="orf3C40" FT CDS complement(1224..1829) FT /codon_start=1 FT /transl_table=11 FT /gene="orf3C40" FT /product="unknown" FT /note="putative exported protein/signal peptide" FT /db_xref="UniProtKB/TrEMBL:Q45442" FT /protein_id="AAC44407.1" FT /translation="MTSKLSKKSISSTVLMGLAAITLSLGIYAPDAKATQSEQVAHLKE FT VETINNKLDQLTDKANAKLKKGLDNVVVQEPLNVQGDKLELSMNVEDISTKAVQQKKFK FT AKVSNTTPVFGFAHEVSGKFTYEKGKLVSSSYDAYLTGVFYSKSHSTKQHKLDPSVWEI FT RSRGTFKALKYTPVEYSSRVDVGLYGSGDYRVLKAKIN" FT gene 2814..3254 FT /gene="orf1.40" FT RBS 2814..2818 FT /gene="orf1.40" FT CDS 2826..3254 FT /codon_start=1 FT /transl_table=11 FT /gene="orf1.40" FT /product="unknown" FT /note="putative exported protein; may consitute a FT functional module with sipP; similar to plasmid pTA1015 FT orf1.15/sipP in GenBank Accession Number U32379" FT /db_xref="UniProtKB/TrEMBL:Q45443" FT /protein_id="AAC44408.1" FT /translation="MKLKFVTMISLAFVLILSPFSMVQADTKDRSISTYKYHGCTTVAK FT KENISGVYLKVSGHGSGRFLLVSVFADANKGKGKRNWVNVSGQDMASLGKYVTKGNKYR FT LTNYAVERYGKNVPIRLCWDAGSKSTGKVSFLWSPDSR" FT gene 3362..3929 FT /gene="sipP40" FT RBS 3362..3366 FT /gene="sipP40" FT CDS 3372..3929 FT /codon_start=1 FT /transl_table=11 FT /gene="sipP40" FT /product="SipP40" FT /note="type I signal peptidase; may constitute a functional FT module with orf1.40; similar to plasmid pTA1015 FT orf1.15/sipP in GenBank Accession Number U32379" FT /db_xref="GOA:Q57350" FT /db_xref="HSSP:1B12" FT /db_xref="InterPro:IPR011056" FT /db_xref="UniProtKB/Swiss-Prot:Q57350" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44409.1" FT /translation="MFDKEKRKKSNIIDWIKAILIALILVFLVRTFLFEPYIVQGESMK FT PTLFNSERLFVNKFVKYTGDFKRGDIVVLNGEEKKTHYVKRLIGLPGDTIEMKNDNLFV FT NGKRFNEEYLKENKKDAHDSDLNLTGDFGPIKVPKDKYFVMGDNRQNSMDSRNGLGLFN FT KKDIVGVEELVFFPLDRIRHAK" FT gene complement(4114..4587) FT /gene="orf2C40" FT CDS complement(4114..4587) FT /codon_start=1 FT /transl_table=11 FT /gene="orf2C40" FT /product="DNA-binding protein" FT /db_xref="GOA:Q45444" FT /db_xref="InterPro:IPR013196" FT /db_xref="UniProtKB/TrEMBL:Q45444" FT /protein_id="AAC44410.1" FT /translation="MKPETYLSSQDIANKLNVSSVTIRKYAAMLEKNGYNFARDTKGWR FT QYNESDLSAMEYIYTHSKLSGKSLEEVAKLVATLYRSNLSISDTATPLQDVNVADLIQR FT QEEFNRAILKRLEQFEEQQKKRDENLMLALKESIEAKKMIAAAQQKKWWQFWK" FT rep_origin 5109..5307 FT /note="palT2 SSO; single strand origin; ssDNA conversion FT signal" FT gene 5943..7070 FT /gene="rap40" FT /note="rap60" FT CDS 5943..7070 FT /codon_start=1 FT /transl_table=11 FT /gene="rap40" FT /product="Rap40" FT /note="putative delay of sporulation onset" FT /db_xref="GOA:Q45445" FT /db_xref="InterPro:IPR013026" FT /db_xref="UniProtKB/TrEMBL:Q45445" FT /protein_id="AAC44411.1" FT /translation="MESIPAAVVGQKINEWYRHIKKLNVTDAEMLREEIKRELDVMEED FT EQAVLYFQLMDFRHEQMLEYVNPSQNQVNKADYLRAVEGEGKKLTGIMEYYVHFFKGMY FT EFSKGEYIKAIAFYRHAEKKLDKVADELERAEFYYKMSEIFYHMKQTHLSMYYIEQAYH FT AYKAHKANGMYEIKVIQCRFVIAGNYDDLCTHEKAIPHLKEAEESARNLGNKYMLAKAL FT LNLGDSYKNMGDQDRALYFLIKAVDQAKESGAKELTQIYYELALLHFKKKEHESGKHYF FT ELGVESAERFNDWFFTNLLNVLKLLYLEGAQPTAVADALEELDDIRGYPYLEELALVSA FT EFYTEIGHMDESVYFYNKMIYAQKQIRRGEFLYEI" FT gene 7060..7179 FT /gene="phr40" FT /note="rap60B" FT CDS 7060..7179 FT /codon_start=1 FT /transl_table=11 FT /gene="phr40" FT /product="Phr40" FT /note="possible regulator of Rap40/Rap60; translationally FT coupled to Rap60d" FT /db_xref="UniProtKB/TrEMBL:Q45446" FT /protein_id="AAC44412.1" FT /translation="MKFNALLLLIVCASLLIVSGSSFVIQQDSNVSVASRKAT" XX SQ Sequence 7837 BP; 2386 A; 1133 C; 1759 G; 2559 T; 0 other; ttgatacata tagaaacaat gagattttca aaaagtgcgt tcaatccagt attgtcaagg 60 gtttgatggc aatttgacag tgaaaaaccc catctgctat tatttaagtg tcgagcaaaa 120 ataatagagt gctagagaac tagcgtcaga aggggttttt cactttgtat tcatctgaat 180 cgaattatag catctttcag gacaaaaccg caacaggtaa aaagcgggat tggaagggga 240 aaaaggtgag atcgttactt atggcggctc attatgaagg acttgaaaag cgtaccggtg 300 cgccttacta cggaaaaaag gcagagaaag tatgtgattg tgcggaatgt cttgcgttta 360 agagagattt agaaacgggc agactacgat tatatcaagc ctatttttgc aaagtgaggt 420 tatgccctat gtgtgcgtgg cgaaggtcat tgaaaattgc ttatcacaac aaattgatcg 480 ttgaagaagc caatcggcaa tataagcccg catggatatt tctcacgttg acagtaaaaa 540 acgttgaggg cgatgatctc aaacagacca tttcggacat gatgaagggt tttaataaat 600 tgatgaaata taagaaagta gattctgcgg tgcgtggata tttcagagct ttagagatca 660 cgaagaatca tgaagaaaat acatatcacc cgcattttca tgtgttgatt cctgttcgta 720 ctggttactt tggtaaaaac tatattaatc aaaaagaatg gacaagcttt tggaagcgtg 780 caatgaaatg ggattacaca ccgattgttc atgtgcagcg tgtcaaagga aaaaagggaa 840 tcgatgcaga agcgattgaa caagaagtgc gtaaagagat ggaagaacag aaggctattc 900 ttgaaatctc gaagtatcct gttaaggata cggatgtcat acgtggtaat gaagtgaccg 960 aggaaaattt ggatacggtc tattacttgg atgatgcatt aagtgccagg cgtttgatcg 1020 ggtacggcgg gatcttgaaa gagattcata aggaactgaa tttgacggat gctgaagacg 1080 gagatctgat ccgtattgaa gaagatgatg atgatgtagc caatgagacg tttgaggtta 1140 tggcgcattg gcatgttggt attaagaatt acataatcac atagaaaagc aggctagaaa 1200 atagcctgct tttcttgtat ggattagttg atttttgctt ttaaaactct ataatctcca 1260 ctgccgtaaa gtcctacgtc aacacgagaa gagtattcaa caggtgtgta tttcagtgct 1320 ttgaaagttc ctctgctacg aatttcccaa acgctagggt ctagtttgtg ttgttttgtt 1380 gagtgtgatt ttgagtaaaa gacgccagtt aaataagcat cataactgct agatactaat 1440 ttgccttttt cataagtaaa tttaccactt acttcatgag caaaaccaaa aacaggagtt 1500 gtatttgaaa cctttgcttt gaatttcttt tgttgtactg cttttgttga aatgtcttca 1560 acgttcatag acaattcgag tttgtctcct tgtacgttta gaggttcttg tactacaacg 1620 ttatctaatc cttttttaag tttggcgttt gctttatcag ttagttgatc tagtttgttg 1680 ttgatcgttt caacttcttt cagatgtgcc acttgttcac tttgtgttgc ttttgcatct 1740 ggggcgtata ttcctaaaga taaagtgatc gcagcaagtc ccattaaaac agtggagctg 1800 attgattttt ttgatagttt tgaagtcata ttgttcaaat cctccttaaa tgatagtatt 1860 acaaacagtt acaaaatttg gttaaataac cacgaataag ggacggtgta ttgtgtatgc 1920 tagctgttat ttatacggtt ttaataatgt ttttggtttt ctatctactg aattatttag 1980 gtcagaaact aattactaaa gatcgcccta ttaatcattc gatcatcata gtgatctcac 2040 ttattgaagc tataattggt atcgttttgg cttactataa accaccgttt ttgtagaatt 2100 gatctataga aatcactcct ttttttgggt atattttcat cctaacattg aatgatttag 2160 cgttgggaat tcattggaaa atttcttttt tggttgcggt gtccgcaatc atttttttta 2220 tttttcagtg cttcgtttta gtggattttt ttgtgataaa atccgttcac tgtggaggtg 2280 aatgtgatga tgattgtaat taatgtgatg gatttaggag cattcatttt ttgtactttg 2340 atgttcattg catgtgcagt ttcagaaaca gaagaagccg gctattgagt cggctttttc 2400 tttacacgct tgacgaaaat cgagagattt tagcaagcac acatctgtgt gtataagtgc 2460 gctattttca taggtgttct atttccaaaa ttgccaccac tttttttgtt tcattggatt 2520 ttttcaaagc gagcatgaga ttttcatctc gctttttttt gctgtttttc agattgttcg 2580 attcgtttta ggatggcccg attgaattcc tcttgtcttt aaatgatcgg cgagcggtat 2640 ccgatatgga caagttcgac caatacaagg gtgctacaag tttggctact tcttctagtc 2700 ttattaggag cctatttttg ttttttctta actttacctt aaaaaaacat taatagataa 2760 ttttatataa ttttaatggt attattttct ttgtaaatta tttctgaaag gatggaagaa 2820 gaactatgaa gttgaaattt gttacaatga tttctttagc ttttgtattg attttatctc 2880 ctttttctat ggtacaagct gatacgaaag atagaagtat aagtacttat aagtatcatg 2940 gttgtaccac tgttgccaaa aaagaaaata tcagtggtgt ttatttgaaa gtatcaggtc 3000 atggttctgg gcgattttta ctcgtttctg tttttgctga tgctaataaa ggaaagggta 3060 aacgaaattg ggtgaacgtt tccggacaag atatggctag tttaggaaaa tatgtaacaa 3120 aaggtaataa atatcgtctt acgaattatg ctgttgaaag atatggtaag aatgttccaa 3180 ttagactttg ttgggatgca ggaagtaaaa gtacggggaa agttagtttc ctttggagcc 3240 cagatagtag ataaatagta agtttctctg tcctgtaaag ataatttctt tacaggattt 3300 tttatttttt gtttaaaaat ctaagaaatg ttaaagttga gttaaaaatt gccaagcggg 3360 aggaagcgta agtgtttgat aaagaaaaaa gaaaaaagtc taatatcatt gattggataa 3420 aagctatttt aatagctctt attcttgttt ttcttgttcg tacttttttg tttgaaccat 3480 acattgtaca aggcgaatct atgaaaccga cattatttaa ttctgaaaga ttatttgtta 3540 ataaatttgt taaatataca ggtgacttta aaagagggga tattgttgtt ttaaatggtg 3600 aggaaaaaaa gacccattat gtgaagagat taattggcct tcctggtgat acaattgaaa 3660 tgaagaatga taatcttttt gtaaatggaa aaaggtttaa tgaggaatac ttaaaagaaa 3720 ataaaaaaga cgcacatgac agtgatctca atttgactgg agattttggt ccaattaagg 3780 tacctaaaga taaatatttt gtcatgggag ataatcgtca aaattcaatg gatagtcgaa 3840 atggtctcgg attatttaat aaaaaggaca ttgttggagt ggaagagtta gttttttttc 3900 ctcttgatcg tatacgtcat gctaaataat atttttatgc ggaagtacct attgcaaaaa 3960 agcaggcatg tattagcctg ctttttttgt atggtctata tctttactat tgtaaagtct 4020 tatgtcagcg tgttttttac aagcttgact gaaatcgaaa gattttagta attacaaaag 4080 ggtgtgtata aatgagacat tccaatggat attctatttc caaaattgcc accacttttt 4140 ttgttgagca gcagcaatca ttttttttgc ttcaatggat tctttcaaag cgagcatgag 4200 attttcatct cgcttttttt gttgttcttc aaattgttcg agtcgtttta ggatggcccg 4260 attgaattct tcttgtcttt gaatgagatc ggcgacattt acatcttgta gcggtgtagc 4320 ggtatccgat atggacaagt ttgaccgata caaggtcgct acaagtttgg ctacttcttc 4380 tagtgattta ccactcaatt tagagtgtgt gtatatatac tccatagcgg ataaatcgga 4440 ctcattatac tgacgccatc ccttagtgtc tctggcgaaa ttatagccgt ttttctcaag 4500 catggcggcg tatttgcgta tcgtgacgct ggagacattg agcttgtttg caatgtcttg 4560 tgaagaaagg taggtttcgg gtttcatagc gggttcacct ctttatgatt cattcgctat 4620 gatatacgct acgccctcct agatcaaaag gcaaaatgtt tttgttttgc atagcgggtt 4680 gaaagagaaa aattttgata agtgtcgctg gagacatttc ggaaaaggtg aaaagagcat 4740 aagggaaaac tgcaaccact ttatgtttga aatcccagtg tgatttgttt cgtggcggtt 4800 gcgatttttg aaaggaatgt gcctaaaaat gaaattctga cacacctttt tttaggagaa 4860 aaatgtggtg atgtggcaga aacggaaaaa ctgccacacc ataaaaagga aaagtgagaa 4920 gaattgagta acgttgtgtt tgaaaaggag cgcacctgca cacaaagaaa gggagcgttt 4980 tgcaaaaaaa tcagtcgtaa caaggggtca ataatgatga attaagtttc gtgtttgttt 5040 tgtgttgttt cggaaaagtg tgcactcaaa aggagcgtta ctgtttaaaa aatggagtga 5100 aaaacatatg gcatgagtca acggtaagcg gaacgaagtg agcattaagg agttgactca 5160 ttcacgacac ccaaaagcct gttaatcaaa caaaccccgt ttgtttgcgc ctgccgaagg 5220 ggcatccccc gaagatcggg ggtagggggt ttgaatattt gccatccaac gtgctagcgt 5280 tggcgggttt gggcagagcc caagaaaggc ggtcggatga tgaaaatgac aagagagcgt 5340 tttgaaaaag gcatgagata tgcgaaagcg acacatgcta ttgaaggaat ttatcttaca 5400 gctgacgagg aagaattatt atggcagcat gcatccggtc agatcacaga tgaggaattt 5460 gagagaaagg ctcttgaatt ggcttataaa gtgatacaat agagttctag gcgaaggtgc 5520 gtagcggaag acgacgacag tcgtcgagtg gcaacgaaaa gcgaagcacc gtagccagct 5580 tgaaccccct cactctaaca ggggggtagt aatgtaagat gaaaataaaa cgtataaaaa 5640 aatatgtcgt gcatttcaaa aatgccgttg agagcgttca gattggattc tgagcgcttt 5700 tatttttgtt taggtaattg aaataggtga aagcgtcaaa aacgcttata agggctttaa 5760 atgcgttttt tcgttttgtc tgctgtcatg attcctaaaa tttgatggtg agataccgag 5820 caaaattcaa aaacggttgc gagataccga aaattttcaa aattggaact ataatggatt 5880 ttataacgta caaattatgc tagcatttaa cataaaaagg gatgtcgaag gagagagatg 5940 tgatggaaag tattcctgct gctgtggtcg gacagaagat taatgaatgg tatcggcata 6000 tcaaaaaatt gaatgtaaca gatgcggaaa tgcttcgtga agagattaaa cgcgaattag 6060 atgtgatgga agaagatgaa caagctgttc tctattttca gttgatggac tttcgtcatg 6120 agcagatgct tgagtatgta aatccttctc aaaatcaagt gaacaaagct gattatttgc 6180 gagctgttga aggtgaaggt aaaaagttga cgggtatcat ggaatactac gttcactttt 6240 ttaaagggat gtatgaattc tctaagggtg agtacataaa agccattgct ttttatagac 6300 atgcagaaaa gaagctggat aaagtagctg atgagcttga acgggctgaa ttttactata 6360 agatgtcaga gattttctat cacatgaagc aaacgcattt gtctatgtat tatatcgagc 6420 aggcgtatca tgcttataaa gctcataaag ccaacgggat gtatgaaatc aaggtgattc 6480 aatgtcgttt tgtcattgca ggaaattatg atgatttatg tacacatgaa aaagcgatac 6540 ctcatttaaa agaagcggaa gagtcggcca ggaatttagg caacaagtat atgcttgcta 6600 aagcgttgct taacctcggt gattcttata aaaatatggg tgatcaggac agagcgcttt 6660 attttttgat caaagctgtt gatcaggcta aagagtctgg agctaaagag cttacacaga 6720 tttattatga gttggcttta ctgcatttta agaaaaagga gcatgaatcg ggaaaacact 6780 attttgagtt gggtgttgaa agtgctgaaa ggttcaatga ttggtttttc acgaatttgt 6840 taaatgtact gaagcttctt tatttagagg gggctcaacc gactgctgtt gcggatgctt 6900 tagaagaact tgacgatatt cgaggctatc catatttaga agaactggcg cttgtgtctg 6960 ctgaatttta tactgaaatt gggcatatgg atgaatctgt atatttttat aacaaaatga 7020 tctatgctca aaaacaaatt cggaggggcg agttcttgta tgaaatttaa tgccttactg 7080 cttttaattg tttgtgctag tttattaatc gttagtggtt cttcttttgt aatccaacaa 7140 gattcaaatg tgtcagttgc ttcaagaaag gctacatgat gatgagtttt attcgatttg 7200 gtacatgaaa agtaacagac ctttagggtc tgtttttttg tggaaatatc atgtgtagtc 7260 acttggaaat aacacgtaaa ctattaggtt tttgattaaa cgtgatattt tcacttggtg 7320 cagccgacag gaacctatcg gccataagat cggaccgaat cgcgatgaaa tgaccttact 7380 tgttctatgc tcttctgttt gtctcttttt caatcccacc atttttgtgg gcgtgtgatg 7440 gcagctgtat gttgaaaggc gggtttagtc aggttttgag tgaaaagcta cagaaaaggt 7500 gttgctgatc gtaaaggcta cagaaacggt gtggtgtttt ttcttggcaa aataaaccct 7560 atgattgcaa ggttttgata tgtatttgag tgattaaaag ctataaaaac ggtgttaaaa 7620 gctaatgaaa agctacaaaa aagtgttgag gctataaaaa cggggggact caatttttga 7680 gtagggggca aaaaatcaaa aaaaaatctc cccctacggg gaaatttggt ttagtctttg 7740 gtcttttagg ttttgaggtt ttggttttta aaaagctggc ggttccgcca gctttttggg 7800 cgattttgac ggagtcagaa tcgtgtcttt tcttatc 7837 // |
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