EBI Dbfetch

ID   U31240; SV 1; linear; mRNA; STD; INV; 1831 BP.
XX
AC   U31240;
XX
DT   08-AUG-1995 (Rel. 44, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Photuris pennsylvanica luciferase mRNA, complete cds.
XX
KW   Firefly luciferase.
XX
OS   Photuris pennsylvanica (Pennsylania firefly)
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera;
OC   Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea;
OC   Lampyridae; Photurinae; Photuris.
XX
RN   [1]
RC   Dr. Keith Wood of Promega Corporation has cloned two cDNAs for Photuris
RC   pennsylvanica from fireflies collected in Maryland.  One of the
RC   corresponding protein sequences differs by only two amino acids from that
RC   reported herein.
RP   1-1831
RX   DOI; 10.1016/S0167-4838(96)00211-7
RX   PUBMED; 9165098.
RA   Ye L., Buck L.M., Schaeffer H.J., Leach F.R.;
RT   "Cloning and sequencing of a cDNA for firefly luciferase from Photuris
RT   pennsylvanica";
RL   Biochim. Biophys. Acta 1339(1):39-52(1997).
XX
RN   [2]
RP   1-1831
RA   Leach F.R.;
RT   ;
RL   Submitted (07-JUL-1995) to the EMBL/GenBank/DDBJ databases.
RL   Biochemistry & Molecular Biology, Oklahoma State University, 246 B Noble
RL   Research Center, Stillwater, OK 74078-3035, USA
XX
CC   On Jun 11, 1997 this sequence version replaced gi:927311.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1831
FT                   /organism="Photuris pennsylvanica"
FT                   /mol_type="mRNA"
FT                   /db_xref="taxon:41716"
FT   5'UTR           1..61
FT   CDS             62..1699
FT                   /codon_start=1
FT                   /product="luciferase"
FT                   /EC_number="1.13.12.7"
FT                   /db_xref="GOA:Q27757"
FT                   /db_xref="HSSP:1LCI"
FT                   /db_xref="InterPro:IPR000873"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q27757"
FT                   /protein_id="AAB60897.1"
FT                   /translation="MEDKNILYGPEPFHPLADGTAGEQMFYALSRYADISGCIALTNAH
FT                   TKENVLYEEFLKLSCRLAESFKKYGLKQNDTIAVCSENGLQFFLPLIASLYLGIIAAPV
FT                   SDKYIERELIHSLGIVKPRIIFCSKNTFQKVLNVKSKLKYVETIIILDLNEDLGGYQCL
FT                   NNFISQNSDINLDVKKFKPNSFNRDDQVALVMFSSGTTGVSKGVMLTHKNIVARFSHCK
FT                   DPTFGNAINPTTAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTFEEKLFLQSLQDYKV
FT                   ESTLLVPTLMAFFPKSALVEKYDLSHLKEIASGGAPLSKEIGEMVKKRFKLNFVRQGYG
FT                   LTETTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGKILGPNETGELYFKGDMIMKS
FT                   YYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAPAEIEGILL
FT                   QHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTAKWLRGGVKF
FT                   LDEIPKGSTGKIDRKVLRQMFEKHKSKL"
FT   3'UTR           1700..>1831
FT   polyA_site      1831
XX
SQ   Sequence 1831 BP; 615 A; 281 C; 353 G; 582 T; 0 other;
     ggcacgagcc agacgggtct ggatagtttg atttaaatac ttaaagttac aagttgaaat        60
     aatggaagat aaaaatattt tatatggacc tgaaccattt catcccttgg ctgatgggac       120
     ggctggagaa cagatgtttt acgcattatc tcggtatgca gatatttcag gatgcattgc       180
     attgacaaat gctcatacaa aagaaaatgt tttatatgaa gaatttttaa aattgtcgtg       240
     tcgtttagcg gaaagtttta aaaagtatgg attaaaacaa aacgacacaa tagcggtgtg       300
     tagtgaaaat ggtttgcaat ttttccttcc tttaattgca tcattgtatc ttggaataat       360
     tgcagcacct gttagtgata aatacattga acgtgaatta atacacagtc ttggtattgt       420
     aaaaccacgc ataatttttt gttccaagaa tacttttcaa aaagtactga atgtaaaatc       480
     taaattaaaa tatgtagaaa ctattattat attagactta aatgaagact taggaggtta       540
     tcaatgcctc aacaacttta tttctcaaaa ttccgatatt aatcttgacg taaagaaatt       600
     taaaccaaat tcttttaatc gagacgatca ggttgcgttg gtaatgtttt cttctggtac       660
     aactggtgtt tctaagggag tcatgctaac tcacaagaat attgttgcac gattttctca       720
     ttgcaaagat cctacttttg gtaacgcaat taatccaacg acagcaattt taacagtaat       780
     accattccac catggttttg gtatgactac cacattagga tactttactt gtggattccg       840
     agttgctcta atgcacacgt ttgaagaaaa actattttta caatcattac aagattataa       900
     agtggaaagt actttacttg taccaacatt aatggcattt tttccaaaaa gtgcgttagt       960
     tgaaaagtac gatttatcgc acttaaaaga aattgcatct ggtggcgcac ctttatcaaa      1020
     agaaattggg gagatggtga aaaaacggtt taaattaaac tttgtcaggc aagggtatgg      1080
     attaacagaa accacttcgg ctgttttaat tacaccggac actgacgtca gaccgggatc      1140
     aactggtaaa atagtaccat ttcacgctgt taaagttgtg gatcctacaa caggaaaaat      1200
     tttggggcca aatgaaactg gagaattgta ttttaaaggc gacatgataa tgaaaagtta      1260
     ttataataat gaagaagcta ctaaagcaat tattaacaaa gacggatggt tgcgctctgg      1320
     tgatattgct tattatgaca atgatggcca tttttatatt gtggacaggc tgaagtcatt      1380
     aattaaatat aaaggttatc aggttgcacc tgctgaaatt gagggaatac tcttacaaca      1440
     cccgtatatt gttgatgccg gcgttactgg tataccggat gaagccgcgg gcgagcttcc      1500
     agctgcaggt gttgtagtac agactggaaa atatctaaac gaacaaatcg tacaaaattt      1560
     tgtttccagt caagtttcaa cagccaaatg gctacgtggt ggggtgaaat ttttggatga      1620
     aattcccaaa ggatcaactg gaaaaattga cagaaaagtg ttaagacaaa tgtttgaaaa      1680
     acacaaatct aagctgtaag gctacgtttc tacgttatct atttatctat ctacacgtta      1740
     tctaattatg tttgagttat aatttatttt atgtatatgt acaattattt atgcttctga      1800
     cttcatcgtc cattaaaagt ggggtgcaat a                                     1831
//