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EBI DbfetchID U27202; SV 1; linear; genomic DNA; STD; PRO; 4312 BP. XX AC U27202; XX DT 03-FEB-1996 (Rel. 46, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Actinobacillus pleuropneumoniae riboflavin biosynthesis operon, DE riboflavin-specific deaminase (ribG), riboflavin synthase alpha subunit DE (ribB), bifunctional GTP cyclohydrase DE II/3,4-dihydroxy-2-butanone-4-phosphate synthase (ribA), and riboflavin DE synthase beta subunit (ribH) genes, complete cds. XX KW . XX OS Actinobacillus pleuropneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. XX RN [1] RP 1-4312 RX PUBMED; 8522537. RA Fuller T.E., Mulks M.H.; RT "Characterization of Actinobacillus pleuropneumoniae riboflavin RT biosynthesis genes"; RL J. Bacteriol. 177(24):7265-7270(1995). XX RN [2] RP 1-4312 RA Fuller T.E., Mulks M.H.; RT ; RL Submitted (16-MAY-1995) to the EMBL/GenBank/DDBJ databases. RL Martha H. Mulks, Microbiology, Michigan State University, 367 Giltner Hall, RL East Lansing, MI 48824, USA XX DR RFAM; RF00050; FMN. XX FH Key Location/Qualifiers FH FT source 1..4312 FT /organism="Actinobacillus pleuropneumoniae" FT /strain="ISU-178; serotype 5" FT /mol_type="genomic DNA" FT /db_xref="taxon:715" FT promoter 106..134 FT RBS 322..326 FT /gene="ribG" FT CDS 330..1562 FT /codon_start=1 FT /transl_table=11 FT /gene="ribG" FT /product="riboflavin-specific deaminase" FT /note="similar to Bacillus subtilis and E. coli RibG, FT Swiss-Prot Accession Numbers P17618 and P25539" FT /db_xref="GOA:P50853" FT /db_xref="InterPro:IPR002734" FT /db_xref="UniProtKB/Swiss-Prot:P50853" FT /protein_id="AAA86522.1" FT /translation="MKLPCKRWFFLSFLQALRSKDFKAFFIIRVNMPVMCFPLPSNSFK FT TMTDLDYMRRAIALAKQGLGWTNPNPLVGCVIVKNGEIVAEGYHEKIGGWHAERNAVLH FT CKEDLSGATAYVTLEPCCHHGRTPPCSDLLIERGIKKVFIGSSDPNPLVAGRGANQLRQ FT AGVEVVEGLLKEECDALNPIFFHYIQTKRPYVLMKYAMTADGKIATGSGESKWITGESA FT RARVQQTRHQYSAIMVGVDTVLADNPMLNSRMPNAKQPVRIVCDSQLRTPLDCQLVQTA FT KEYRTVIATVSDDLQKIEQFRPLGVDVLVCKARNKRVDLQDLLQKLGEMQIDSLLLEGG FT SSLNFSALESGIVNRVHCYIAPKLVGGKQAKTPIGGEGIQQIDQAVKLKLKSTELIGED FT ILLDYVVISPL" FT RBS 1675..1678 FT /gene="ribB" FT CDS 1685..2332 FT /codon_start=1 FT /transl_table=11 FT /gene="ribB" FT /product="riboflavin synthase alpha subunit" FT /note="lumazine synthase; similar to Bacillus subtilis FT RibB, Swiss-Prot Accession Number P16440, and E. coli RibC, FT Swiss-Prot Accession Number P29015" FT /db_xref="GOA:P50854" FT /db_xref="HSSP:1I8D" FT /db_xref="InterPro:IPR001783" FT /db_xref="UniProtKB/Swiss-Prot:P50854" FT /protein_id="AAA86523.1" FT /translation="MFTGIIEEVGKIAQIHKQGEFAVVTINATKVLQDVHLGDTIAVNG FT VCLTVTSFSSNQFTADVMSETLKRTSLGELKSNSPVNLERAMAANGRFGGHIVSGHIDG FT TGEIAEITPAHNSTWYRIKTSPKLMRYIIEKGSITIDGISLTVVDTDDESFRVSIIPHT FT IKETNLGSKKIGSIVNLENDIVGKYIEQFLLKKPADEPKSNLSLDFLKQAGF" FT RBS 2382..2385 FT /gene="ribA" FT CDS 2393..3598 FT /codon_start=1 FT /transl_table=11 FT /gene="ribA" FT /product="GTP cyclohydrase II/ FT 3,4-dihydroxy-2-butanone-4-phosphate synthase" FT /note="bifunctional enzyme; similar to Bacillus subtilis FT RibA, Swiss-Prot Accession Number P17620; 5' region FT similar to E. coli RibB, Swiss-Prot Accession Number FT P24199; 3' region similar to E. coli RibA, Swiss-Prot FT Accession Number P25523" FT /db_xref="GOA:P50855" FT /db_xref="HSSP:1G58" FT /db_xref="InterPro:IPR016299" FT /db_xref="UniProtKB/Swiss-Prot:P50855" FT /protein_id="AAA86524.1" FT /translation="MTDFQFSKVEDAIEAIRQGKIILVTDDEDRENEGDFICAAEFATP FT ENINFMATYGKGLICTPISTEIAKKLNFHPMVAVNQDNHETAFTVSVDHIDTGTGISAF FT ERSITAMKIVDDNAKATDFRRPGHMFPLIAKEGGVLVRNGHTEATVDLARLAGLKHAGL FT CCEIMADDGTMMTMPDLQKFAVEHNMPFITIQQLQEYRRKHDSLVKQISVVKMPTKYGE FT FMAHSFVEVISGKEHVALVKGDLTDGEQVLARIHSECLTGDAFGSQRCDCGQQFAAAMT FT QIEQEGRGVILYLRQEGRGIGLINKLRAYELQDKGMDTVEANVALGFKEDEREYYIGAQ FT MFQQLGVKSIRLLTNNPAKIEGLKEQGLNIVAREPIIVEPNKNDIDYLKVKQIKMGHMF FT NF" FT promoter 3653..3682 FT RBS 3698..3701 FT /gene="ribH" FT CDS 3709..4170 FT /codon_start=1 FT /transl_table=11 FT /gene="ribH" FT /product="riboflavin synthase beta subunit" FT /note="similar to Bacillus subtilis and E. coli RibH, FT Swiss-Prot Accession Numbers P11998 and P25540" FT /db_xref="GOA:P50856" FT /db_xref="HSSP:1RVV" FT /db_xref="InterPro:IPR002180" FT /db_xref="UniProtKB/Swiss-Prot:P50856" FT /protein_id="AAA86525.1" FT /translation="MAKITGNLVATGLKFGIVTARFNDFINDKLLSGAIDTLVRHGAYE FT NDIDTAWVPGAFEIPLVAKKMANSGKYDAVICLGTVIRGSTTHYDYVCNEAAKGIGAVA FT LETGVPVIFGVLTTENIEQAIERAGTKAGNKGSECALGAIEIVNVLKAI" FT terminator 4194..4247 XX SQ Sequence 4312 BP; 1334 A; 774 C; 1020 G; 1184 T; 0 other; aattcggtcg gacgtacttt atttgagcat atcaatgaag gaggttttga ttatgtgatt 60 tcagagtgtg aaacctgtaa atggcagatt gatatgtcga gcaatgtgac ttgtttacat 120 ccgattactt tattatcaat ggcattggat aaacgctaat tcttgcttga ctttgacaat 180 caaaagtcgc aaatttgcaa caatttttta ataatcttca gggcagggtg aaattcccga 240 tcggcggtaa agtccgcgag ccgaacgaaa aaggtttggc aggaaccggt gagattccgg 300 taccgacagt atagtctgga tggaagaaga tgaaattacc gtgtaagcgg tggtttttcc 360 tatctttttt acaagccttg agatcgaaag atttcaaggc ttttttcatc attagggtaa 420 acatgcctgt aatgtgtttt cctctgccct caaatagttt caaaacaatg acggatttag 480 actatatgcg ccgtgccatt gcactggcaa aacaaggttt aggctggacg aatcccaatc 540 cgcttgtcgg ttgtgtaatt gtcaaaaacg gtgaaatcgt tgccgaaggt taccatgaaa 600 agattggtgg atggcatgcg gaacgtaatg ccgttttaca ttgtaaggaa gatctttccg 660 gggcgactgc ttatgtaacg cttgagcctt gttgtcatca cggccgcacg ccgccttgtt 720 cggatttatt aattgaacga ggcattaaaa aagtatttat cggttcgagc gatccgaatc 780 ctttagtagc agggcgggga gcaaatcagc tacgccaagc cggcgtggaa gtggtggaag 840 gtttactcaa agaagaatgt gatgcgttaa acccgatttt tttccactat attcaaacta 900 aacgtccgta tgtgctaatg aaatatgcca tgacggcaga cggcaaaatt gcaaccggta 960 gcggcgaatc caaatggatt accggtgaat cggcaagagc aagagtgcag caaacacgtc 1020 atcaatatag tgcgattatg gtcggtgtag atacggtact tgccgataac ccgatgttaa 1080 atagccgaat gccgaatgcg aaacaaccgg tccggattgt ctgcgatagc caattacgta 1140 caccgttaga ttgccagtta gtgcagacag cgaaagaata tcgcaccgta attgcaaccg 1200 ttagtgacga tttgcaaaaa attgaacaat ttagaccgct tggcgtagat gtattagtgt 1260 gtaaagcacg aaacaagcgg gtagatttgc aagatctttt gcaaaagctc ggtgaaatgc 1320 agatcgacag cctcttattg gaaggcggtt caagtttgaa tttcagtgcg ttagaaagcg 1380 gtatcgtgaa tcgagtacat tgttatattg cgcctaaatt agtcggtggt aaacaagcga 1440 aaaccccaat cggcggtgag ggaattcaac aaatcgacca agcggttaaa ttaaaattga 1500 aatcgaccga actcatcggc gaagatattt tgttggatta tgtagtcatc tcccctcttt 1560 agcaaagagg ggtcggggga gatttgagat aatgttgaaa tttacaccgc ctttcacttt 1620 ggcgttgtta aatctcccct aacccctctt tacaaaagag agggatcaat aatgaggaaa 1680 ttatatgttc acaggtatta ttgaagaagt cggcaaaatt gctcaaattc ataagcaagg 1740 cgaatttgcg gtagtcacaa ttaatgcgac caaagtatta caagacgttc atttaggcga 1800 cacgattgcg gtgaacggcg tatgtttaac cgtaacttct ttttcgagta atcagtttac 1860 cgccgatgta atgtcggaaa cgttaaaacg tacttcatta ggcgaattaa agtcgaatag 1920 tccggttaat ttagaacgcg cgatggcggc aaacggacgt ttcggcggac acatcgtttc 1980 ggggcatatt gacggcaccg gcgaaattgc ggaaatcaca ccggcacata attcgacatg 2040 gtatcgcatt aaaacctctc caaaattaat gcgttatatt attgagaaag gttcgatcac 2100 cattgacggt attagcctga ccgtagtcga taccgatgat gaaagtttcc gtgtatcgat 2160 tattccgcat acgattaaag aaaccaattt aggttcgaaa aaaatcggca gtattgtcaa 2220 tttagaaaat gatattgtcg gtaaatatat cgaacagttt ttactgaaaa agccggcgga 2280 tgagccgaaa agtaatctta gtttagactt tttaaagcag gcgggatttt aagatttgta 2340 ggacacactg agtgtatcct accgacaaaa atatatattt taggaaaaga agatgacaga 2400 tttccaattt tcaaaagtag aagatgcgat cgaagcgatt cgacaaggca aaatcatttt 2460 agtgactgac gatgaagatc gcgaaaacga aggcgatttt atctgtgcgg cggaatttgc 2520 cacaccggaa aatatcaatt ttatggcaac ttacggcaaa ggtttgattt gtacgccgat 2580 ttcaaccgaa atcgctaaaa aattaaattt ccatccgatg gttgcggtca atcaagataa 2640 tcatgaaacg gcgtttaccg tatcggtgga tcatattgat acgggaacgg gtatctcagc 2700 ttttgagcgt tcgattaccg caatgaaaat tgtcgatgat aatgctaaag caacggattt 2760 ccgccgcccg gggcatatgt ttccgttaat cgctaaagaa ggtggagtgt tagtgcgtaa 2820 cggtcatacc gaagcaacag tggatttagc tcgtttagcc ggtttaaaac acgccggttt 2880 atgttgtgaa attatggcgg atgacggcac gatgatgact atgccggatc tacaaaaatt 2940 tgcggtagaa cacaatatgc cgtttatcac gattcaacaa ttacaagaat atcgccgtaa 3000 gcatgacagc ttggtgaaac aaatttctgt ggtaaaaatg ccgacaaaat acggtgagtt 3060 tatggcacat agctttgttg aagtgatttc aggtaaagaa cacgttgcgt tagtcaaagg 3120 cgatttaacc gacggtgagc aagtattggc gcgtatccat tcggaatgtt taaccggtga 3180 cgctttcggt tctcaacgtt gtgattgcgg tcagcaattt gccgcagcaa tgacccaaat 3240 tgagcaagag ggcagaggtg tgattctgta tttacgccaa gaaggtcgtg gtatcggttt 3300 aatcaataag ctacgtgctt acgaactaca agataaaggg atggataccg ttgaagcgaa 3360 cgtcgcttta ggatttaaag aagacgaacg tgagtactat atcggtgcac aaatgttcca 3420 gcagttaggc gtaaaatcga tccgtttatt aaccaataat ccggcaaaaa ttgaaggctt 3480 aaaagagcaa ggattaaata tcgttgcacg tgagccgatt attgtagaac cgaacaaaaa 3540 tgacattgat tacctaaaag tcaaacagat aaaaatgggg catatgttta acttctaact 3600 ttaacaaccg tatgtagtat tagggaagca agcgttgcgt ccctactata gaatgataca 3660 agcggtcact tttttataaa attttgcata tttcgagagg acaaaaaaat ggcaaagatt 3720 acaggtaact tagttgcgac aggtttaaaa ttcggtattg taaccgcacg tttcaacgat 3780 tttatcaacg ataaattatt aagcggtgca attgatacgt tagtgcgtca cggtgcgtat 3840 gaaaacgata ttgatacggc atgggttccg ggtgcatttg agattccatt agttgcgaaa 3900 aaaatggcaa acagcggtaa atatgatgcg gtaatctgtt taggtacggt aattcgcggt 3960 tcgacaactc actatgatta cgtatgtaat gaagcggcaa aaggtatcgg tgcggtagca 4020 ttagaaaccg gcgtaccggt aattttcggt gtattaacca cagaaaatat tgaacaggcg 4080 attgaacgcg cgggtactaa agcaggtaat aaaggttcag aatgtgcatt aggcgcaatc 4140 gaaatagtaa acgtattaaa agcgatctaa ttttcgtttg acgtgctaaa aacaagcggt 4200 cgtttttgac tggaattttg caaatttccc gttaaaaacg accgcttata ttttatgtct 4260 agtaaagacc ttctttctcg taccagattt tgttgatata tagcaagctt gg 4312 // |
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