EBI Dbfetch

ID   U27084; SV 1; linear; genomic DNA; STD; PRO; 1553 BP.
XX
AC   U27084;
XX
DT   13-JUN-1995 (Rel. 44, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   Bacillus sp. bifunctional cellulase precursor (cel) gene, complete cds.
XX
KW   .
XX
OS   Bacillus sp.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-1553
RX   DOI; 10.1074/jbc.270.43.26012
RX   PUBMED; 7592793.
RA   Han S.J., Yoo Y.J., Kang H.S.;
RT   "Characterization of a bifunctional cellulase and its structural gene. The
RT   cell gene of Bacillus sp. D04 has exo- and endoglucanase activity";
RL   J. Biol. Chem. 270(43):26012-26019(1995).
XX
RN   [2]
RP   1-1553
RA   Kang H.S.;
RT   ;
RL   Submitted (14-MAY-1995) to the EMBL/GenBank/DDBJ databases.
RL   Hyen S. Kang, Seoul National University, Department of Microbiology,
RL   Shillim-Dong SAN 56-1, Gwanak-Gu, Seoul, 151-742, South Korea
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1553
FT                   /organism="Bacillus sp."
FT                   /strain="D04"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1409"
FT   -35_signal      5..11
FT                   /gene="cel"
FT   -10_signal      28..32
FT                   /gene="cel"
FT   RBS             73..79
FT                   /gene="cel"
FT   sig_peptide     92..178
FT                   /gene="cel"
FT   CDS             92..1552
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="cel"
FT                   /product="bifunctional cellulase precursor"
FT                   /function="cellulose degradation; exo- and endoglucanase"
FT                   /db_xref="GOA:Q45430"
FT                   /db_xref="HSSP:1LF1"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/TrEMBL:Q45430"
FT                   /protein_id="AAC43478.1"
FT                   /translation="MKRSISIFITCLLITLLTMGGMIRSPASAAGTKTPVAKNGQLSIK
FT                   GTQLVNRDGKAVQLKGISSHGLQWYGEYVNKDSLKWLRDDWGITVFRAAMYTADGGYID
FT                   NPSVKNKVKEAVEAAKELGIYVIIDWHILNDGNPNQNKEKAKEFFKEMSSLYGNTPNVI
FT                   YEIANEPNGDVNWKRDIKPYAEEVISVIRKNDPDNIIIVGTGTWSQDVNDAADDQLKDA
FT                   NVMYALHFYAGTHGQFLRDKANYALSKGAPIFVTEWGTSDASGNGGVFLDQSREWLKYL
FT                   DSKTISWVNWNLSDKQESSSALKPGASKTGGWRLSDLSASGTFVRENILGTKDSTKDIP
FT                   ETPSKDKPTQENGISVQYRAGDGSMNSNQIRPQLQIKNNGNTTVDLKDVTARYWYNAKN
FT                   KGQNLDCDYEQLGCGNVSHTVVTLHKPKQGADTYLELGFKNGTLAPGASTGNIQLRLHN
FT                   DDWSNYAQSGDYSFFKSNTFKTMKKITI"
XX
SQ   Sequence 1553 BP; 527 A; 303 C; 370 G; 353 T; 0 other;
     ttgatagaca atcgggagaa agatttttac aatgagttcg tgctcataag aagtgtagag        60
     ccaaaatatg ccaaggagga agagatcaga tatgaaacgg tcaatctcta tttttattac       120
     gtgtttattg attacgttat tgacaatggg cggcatgata cgttcgccgg catcagcagc       180
     agggacaaaa acgccagtag ccaagaatgg ccagcttagc ataaaaggta cacagctcgt       240
     taaccgagac ggtaaagcgg tacagctgaa ggggatcagt tcacacggat tgcaatggta       300
     tggagaatat gtcaataaag acagcttaaa atggctgaga gatgattggg gtatcaccgt       360
     tttccgtgca gcgatgtata cggcagatgg cggttatatt gacaacccgt ccgtgaaaaa       420
     taaagtaaaa gaagcggttg aagcggcaaa agagcttggg atatatgtca tcattgactg       480
     gcatatctta aatgacggta atccaaacca aaataaagag aaggcaaaag aattcttcaa       540
     ggaaatgtca agcctttacg gaaacacgcc aaacgtcatt tatgaaattg caaacgaacc       600
     aaacggtgat gtgaactgga agcgtgatat taaaccatat gcggaagaag tgatttcagt       660
     tatccgcaaa aatgatccag acaacatcat cattgtcgga accggtacat ggagccagga       720
     tgtgaatgat gctgccgatg accagctaaa agatgcaaac gttatgtacg cacttcattt       780
     ttatgccggc acacacggcc aatttttacg ggataaagca aactatgcac tcagcaaagg       840
     agcacctatt tttgtgacag agtggggaac aagcgacgcg tctggcaatg gcggtgtatt       900
     ccttgatcaa tcgagggaat ggctgaaata tctcgacagc aagaccatca gctgggtgaa       960
     ctggaatctt tctgataagc aggaatcatc ctcagcttta aagccggggg catctaaaac      1020
     aggcggctgg cggttgtcag atttatctgc ttcaggaaca ttcgttagag aaaacattct      1080
     cggcaccaaa gattcgacga aggacattcc tgaaacgcca tcaaaagata aacccacaca      1140
     ggaaaatggt atttctgtac agtacagagc aggggatggg agtatgaaca gcaaccaaat      1200
     ccgtccgcag cttcaaataa aaaataacgg caataccacg gttgatttaa aagatgtcac      1260
     tgcccgttac tggtataacg cgaaaaacaa aggccaaaac cttgactgtg actacgagca      1320
     gcttggatgc ggcaatgtgt cacacacagt tgtgacgttg cataaaccaa agcaaggtgc      1380
     agatacctat ctggaacttg gatttaaaaa cggaacgttg gcaccgggag caagcacagg      1440
     gaatattcag ctccgtcttc acaatgatga ctggagcaat tatgcacaaa gcggcgatta      1500
     ttcctttttc aaatcaaata cgtttaaaac aatgaaaaaa atcactatat gat             1553
//