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EBI DbfetchID U24701; SV 1; linear; genomic DNA; STD; FUN; 3389 BP. XX AC U24701; XX DT 22-MAY-1995 (Rel. 43, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE Septoria lycopersici beta-1,2-D-glucosidase (B2Tom) gene, complete cds. XX KW . XX OS Septoria lycopersici (tomato leaf spot fungus) OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; OC Dothideomycetidae; Capnodiales; Mycosphaerellaceae; OC mitosporic Mycosphaerellaceae; Septoria. XX RN [1] RP 1-3389 RX PUBMED; 8664504. RA Sandrock R.W., DellaPenna D., VanEtten H.D.; RT "Purification and characterization of beta 2-tomatinase, an enzyme involved RT in the degradation of alpha-tomatine and isolation of the gene encoding RT beta 2-tomatinase from Septoria lycopersici"; RL Mol. Plant Microbe Interact. 8(6):960-970(1995). XX RN [2] RP 1-3389 RA Sandrock R.W.; RT ; RL Submitted (12-APR-1995) to the EMBL/GenBank/DDBJ databases. RL Robert W. Sandrock, Plant Pathology, University of Arizona, Rm 204 Forbes RL Hall, Tucson, AZ 85721, USA XX FH Key Location/Qualifiers FH FT source 1..3389 FT /organism="Septoria lycopersici" FT /mol_type="genomic DNA" FT /clone_lib="pKBY2 library of R. Sandrock" FT /clone="pKBY2#6.1" FT /db_xref="taxon:39703" FT 5'UTR 1..633 FT exon 1..739 FT /number=1 FT CDS join(634..739,794..982,1041..1176,1237..3217) FT /codon_start=1 FT /gene="B2Tom" FT /product="beta-1,2-D-glucosidase" FT /function="hydrolysis of beta-1,2-glucose residue from FT tetrasaccharide moeity of alpha-tomatine" FT /EC_number="3.2.1.21" FT /note="involved in the detoxification of alpha-tomatine FT found in Lycopersicon spp.; belongs to family 3 of FT glycosyl-hydrolases; B2-tomatinase" FT /db_xref="GOA:Q99324" FT /db_xref="InterPro:IPR001764" FT /db_xref="InterPro:IPR002772" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q99324" FT /protein_id="AAB08445.1" FT /translation="MVSSLFNIAALAGAVIALSHEDQSKHFTTIPTFPTPDSTGEGWKA FT AFEKAADAVSRLNLTQKVALTTGTTAGLSCNGNIAPIPEINFSGLCLADGPVSVRIADL FT ATVFPAGLTAAATWDRQLIYERARALGSEFRGKGSQVHLGPASGALGRHPLGGRNWESF FT SPDPYLSGVAMDFSIRGIQEMGVQANRKHFIGNEQETQRSNTFTDDGTEIQAISSNIDD FT RTMHELYLWPFANAVRSGVASVMCSYNRLNQTYACENSKLMNGILKGELGFQGYVVSDW FT YATHSGVESVNAGLDMTMPGPLDSPSTALRPPPSYLGGNLTEAVLNGTIPEARVDDMAR FT RILMPYFFLGQDTDFPTVDPSTGFVFARTYNYPDEYLTLGGLDPYNPPPARDVRGNHSD FT IVRKVAAAGTVLLKNVNNVLPLKEPKSVGIFGNGAADVTEGLTFTGDDSGPWGADIGAL FT SVGGGSGAGRHTHLVSPLAAIRKRTESVGGRVQYLLSNSRIVNDDFTSIYPTPEVCLVF FT LKTWAREGTDRLSYENDWNSTAVVNNVARRCPNTIVVTHSGGINTMPWADNANVTAILA FT AHYPGQENGNSIMDILYGDVNPSGRLPYTIPKLATDYDFPVVNITNEAQDPYVWQADFT FT EGLLIDYRHFDARNITPLYEFGYGLSYTTFEIEGVANLVAKSAKLSAFPASTDISHPGG FT NPDLWEEVVSVTAAVKNTGSVSGSQVVQLYISLPADGIPENSPMQVLRGFEKVDLQPGQ FT SKSVEFSIMRRDLSFWNTTAQDWEIPNGQIEFRVGFSSRDIKSIVSRSFL" FT exon 794..982 FT /gene="B2Tom" FT /number=2 FT exon 1041..1176 FT /gene="B2Tom" FT /number=3 FT exon 1237..3389 FT /number=4 FT misc_feature 1469..1471 FT /gene="B2Tom" FT /note="encodes active site" FT /inference="non-experimental evidence, no additional FT details recorded" FT 3'UTR 3215..3389 XX SQ Sequence 3389 BP; 776 A; 949 C; 872 G; 792 T; 0 other; ctgcagcagc agacatccgc agatcaaccc gacgatatgc gcccggatcc gagcagagca 60 ccgagcctac gaccatcaga ggacgcttgt ggcaaagagt tccatagcgt cttacgcccg 120 gatacatatt acgttgtagc tgggtggggc aggggcatca tccaagactt gcgttcctcg 180 gcctctacag actctgtgaa gaatcattgc aacccctgct agcgtagggt cctagactca 240 ctatatggag gagctgtaaa cctggccctg ctcgaataga gagaacctct ttgttgaagg 300 aattgcaatt gtgtcttctt gatagtaaga acacggatga gttaggactc aggagaaagt 360 aaccactcaa tgtagttagc attccacgct agcaggggtt cgaaagattt ttcacagagt 420 ctgtattgtg gtacgtactc gtatttcgta tcgaatacgt gcgtcctttg ccaaacgatg 480 gcgacaattc ggccgaacat taacggttgc ctaaaggttt agttagatcc aaacgcaagg 540 tccacgagag cgctgtaaca aaagccagta catcctccaa caattggcat atccaacgag 600 ctgctttacg agactccgcg cgttgacatt ataatggtat caagcttgtt caacatcgct 660 gcccttgctg gggccgtgat cgccttgtcc cacgaagatc aatcgaagca ctttacgact 720 attccgacat tcccaacacg tacgtggcag acttgcgtgt tgcgatgccg atacactgac 780 tccctgggcg cagctgatag cacgggcgaa ggatggaagg ccgcctttga gaaagcggcc 840 gatgccgtgt cgcgtctcaa cctcacgcag aaggttgcgc tgacgacggg cacgacagct 900 ggcctttcct gcaacggaaa catcgctccg attcctgaga tcaatttcag tggcttgtgt 960 ttggcagatg gaccagtatc gggtgagtga cacatgttcc gcgcaccgtt tgttcactga 1020 ctttcgctgt ccggaccagg tccgaattgc agaccttgcc acggtatttc ccgcaggcct 1080 gacggctgct gcaacttggg accgccagct gatctacgaa cgcgcgcgag cactgggttc 1140 tgagttccgt ggcaaaggat ctcaagtgca cctggggtga gtctgcttca ccctttctcg 1200 gatcgatcca ttgagagaag gctgactgga cttcagacca gcgtctggcg cattgggtcg 1260 tcatccgttg gggggccgca actgggagag cttcagtcct gatccctacc tcagtggggt 1320 ggccatggac ttctccatcc gtggcattca ggaaatgggc gttcaagcga accgcaagca 1380 ctttatcggc aatgaacaag aaacacagcg ctccaatacc tttacggatg acggtactga 1440 gattcaggca atctcatcca acatcgacga ccgaaccatg cacgagttat atctctggcc 1500 cttcgccaac gctgtccgct cgggcgttgc ctcagtcatg tgcagttaca acagacttaa 1560 ccagacatat gcttgcgaga actcgaagct catgaacggt attcttaaag gcgagttagg 1620 cttccaggga tatgttgtca gcgactggta cgccacccac tcaggagtcg agtccgtcaa 1680 tgcaggtctg gacatgacga tgcctggccc tctcgacagt ccgtcaaccg ctttacgacc 1740 gccgccatcc tatcttggtg gcaacttgac cgaagccgtt ctcaatggta ctatacccga 1800 ggcaagggtc gacgacatgg cacgccgcat cctcatgcca tacttttttc ttggccaaga 1860 cacggacttt ccaactgtcg acccctcgac cggttttgtc ttcgctagaa catacaacta 1920 cccggatgaa tacttgacac ttggaggctt ggatccttac aaccccccgc ctgctcgcga 1980 tgtgcgtggt aaccattctg atattgtgcg gaaggttgcc gcagccggga cggtccttct 2040 gaagaacgtg aacaacgttc ttcccctcaa agaacccaag tccgttggta tattcggcaa 2100 cggtgctgcc gatgttactg aaggtcttac attcaccgga gatgacagcg gaccttgggg 2160 tgccgatatc ggtgccctta gcgttggcgg aggatccggt gccggtagac acacacacct 2220 tgtttctccc cttgcagcga ttcgcaagcg cacggaaagc gtggggggtc gcgtccagta 2280 cttgctcagc aactccagga ttgtaaacga cgacttcaca tcgatctacc ccacgccgga 2340 ggtttgccta gtttttctga aaacctgggc tcgtgagggc actgatcgtt tgtcgtacga 2400 gaatgactgg aactcaactg ccgttgtaaa caatgtggct cgccggtgtc ccaataccat 2460 tgtcgtcacc cactccggag gcatcaacac catgccctgg gcggacaatg ccaatgtgac 2520 ggcgatactc gccgcgcact accctggtca ggaaaatggg aactctataa tggatatctt 2580 gtacggagat gtcaatccat ctggtcgcct gccctacacg atacccaagc tggccaccga 2640 ctacgacttc ccggttgtca acataactaa tgaggctcag gacccatatg tttggcaagc 2700 cgacttcacg gaaggtctct tgatcgatta ccgtcacttt gatgctagga acattacccc 2760 tctttatgag tttggctatg ggctcagtta caccactttc gagatcgagg gcgtcgcaaa 2820 tctggtagca aaatccgcca aactctccgc cttccctgct tcaacggaca tctcccaccc 2880 cggaggtaat cctgacctgt gggaagaggt ggtttcggta accgccgcag taaagaacac 2940 aggcagcgtc tccgggtcgc aggtggtgca gctgtatatt tctctgcccg cggatggtat 3000 acctgagaat tcgccaatgc aggttttacg cggcttcgag aaggttgatc tccaaccggg 3060 acagtccaaa tccgtcgaat tctcgattat gcgccgcgac ctgagtttct ggaacacgac 3120 ggcgcaggac tgggagattc cgaacggcca gatcgagttc cgtgttggct ttagctcaag 3180 agacatcaag tctatcgttt caagatcttt tctatagagt cctatgttac ggtttggtgc 3240 gccggcccag aggctcggca gatgccatac cgcctcacct cgcccacctc tcccaacata 3300 gccgccgaga agctctaaat agaagctgct acctttgcct ggttgtccac gtactgtcca 3360 agcatcgcga ttcccaacta tggcctttc 3389 // |
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