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EBI DbfetchID U24268; SV 1; linear; genomic DNA; STD; PRO; 4229 BP. XX AC U24268; XX DT 10-AUG-1995 (Rel. 44, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Clostridium litorale glycine reductase complex selenoproteins A (grdA) and DE B (grdB), thioredoxin reductase (trxB), and thioredoxin (trxA) genes, DE complete cds. XX KW . XX OS Clostridium litorale OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-4229 RA Kreimer S., Soehling B., Andreesen J.R.; RT "Site directed mutagenesis of thioredoxin from Clostridium litorale: the RT unusual Gly31 at the redox active site is essential for activity"; RL Unpublished. XX RN [2] RP 1-4229 RX DOI; 10.1111/j.1432-1033.1995.192_c.x RX PUBMED; 8529640. RA Kreimer S., Andreesen J.R.; RT "Glycine reductase of Clostridium litorale. Cloning, sequencing, and RT molecular analysis of the grdAB operon that contains two in-frame TGA RT codons for selenium incorporation"; RL Eur. J. Biochem. 234(1):192-199(1995). XX RN [3] RP 1-4229 RA Kreimer S., Andreesen J.R.; RT ; RL Submitted (10-APR-1995) to the EMBL/GenBank/DDBJ databases. RL Sylke Kreimer, Institut fuer Mikrobiologie, Georg-August-Universitaet, RL Grisebachstrasse 8, Goettingen, D-37077, Germany XX CC On Jan 30, 1996 this sequence version replaced gi:924752. XX FH Key Location/Qualifiers FH FT source 1..4229 FT /organism="Clostridium litorale" FT /mol_type="genomic DNA" FT /db_xref="taxon:1557" FT -35_signal 393..398 FT -10_signal 424..429 FT RBS 436..440 FT CDS 450..926 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:579..581,aa:OTHER) FT /gene="grdA" FT /product="glycine reductase complex selenoprotein A" FT /note="selenocysteine" FT /db_xref="GOA:P52216" FT /db_xref="InterPro:IPR006812" FT /db_xref="UniProtKB/Swiss-Prot:P52216" FT /protein_id="AAC43573.2" FT /translation="MSLFDGKKVIIIGDRDGIPGPAMAECLKGINVEVVYSATECFVXT FT AAGAMDLENQNWVKNFTDQYGAENIIVLVGAAEAESAGLAAETVTAGDPTFAGPLAGVQ FT LGLRVFHAVEPEFKGAVDSAIYDEQIGMMEMVLDVDSIIEEMKSIRADYCKFND" FT RBS 932..936 FT CDS 944..2254 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:1991..1993,aa:OTHER) FT /gene="grdB" FT /product="glycine reductase complex selenoprotein B" FT /note="selenocysteine" FT /db_xref="GOA:P52217" FT /db_xref="InterPro:IPR010187" FT /db_xref="UniProtKB/Swiss-Prot:P52217" FT /protein_id="AAC43574.2" FT /translation="MGKLRVVHYINQFFAGIGGEEKADIAPFVAEELPPISQNLDKLLG FT EDAEVVATVVCGDSFFGENLETAQATVLEMVKGASPDLFIAGPAFNAGRYGVAAGAITK FT AVQDTLGIPAVTGMYIENPGADMYKKSVYVMSTADSAAGMRKSLPALAKFALKYAKGEE FT IGSPAEEGYIERGIRVNGFKEDRGAKRAVAMLVKKLKGEEFVTEYPMPVFDNVVPGRAI FT VNMSKAKIAIVTSGGIVPKGNPDRIESSSASKYGKYDIDGVDDLTSEGWETAHGGHDPV FT YANEDADRVIPVDVLRDMEKEGVIGELHRYFYSTTGNGTAVLSSKQFAKEFTQELMAAG FT VDAVILTSTXGTCTRCGATMVKEIERSGIPVVHICTVTPIALTVGANRIVPAIAIPHPL FT GDPALSPAEEKALRRKIVEKSLKALETEIEEQTVFED" FT stem_loop 2298..2329 FT stem_loop 2326..2392 FT -35_signal 2385..2390 FT -10_signal 2405..2410 FT RBS 2457..2461 FT /gene="trxB" FT CDS 2470..3417 FT /codon_start=1 FT /transl_table=11 FT /gene="trxB" FT /product="thioredoxin reductase" FT /db_xref="GOA:P52213" FT /db_xref="HSSP:1VDC" FT /db_xref="InterPro:IPR005982" FT /db_xref="UniProtKB/Swiss-Prot:P52213" FT /protein_id="AAC43575.1" FT /translation="MENVYDIAIIGSGPAGLAAALYGARAKMKTLLLEGMKVGGQIVIT FT HEVANYPGSVPEATGPSLIGRMEEQVEEFGAERVMDNIVDVDFTDKIKVLKGAKGEYKA FT KAVIVATGASPKLAGCPGEKELTGKGVSYCATCDADFFEDMEVFVIGGGDTAVEEAMFL FT TKFARKVTIVHRRAELRAAKSIQEKAFKNEKLNFMWNTVIEEIKGDGIVESAVFKNRET FT GEVTEFVAPEEDGTFGIFVFIGYDPKSALVEGKLELDETGYIPTDDNMKTNVEGVFAAG FT DIRVKSLRQVVTATADGAIAAVQAEKYIEELFAE" FT RBS 3519..3523 FT /gene="trxA" FT CDS 3532..3855 FT /codon_start=1 FT /transl_table=11 FT /gene="trxA" FT /product="thioredoxin" FT /db_xref="GOA:P21609" FT /db_xref="HSSP:1TRV" FT /db_xref="InterPro:IPR012335" FT /db_xref="UniProtKB/Swiss-Prot:P21609" FT /protein_id="AAC43576.1" FT /translation="MLMLDKDTFKTEVLEGTGYVLVDYFSDGCVPCKALMPAVEELSKK FT YEGRVVFAKLNTTGARRLAISQKILGLPTLSLYKDGVKVDEVTKDDATIENIEAMVEEH FT ISK" FT stem_loop 3926..3958 XX SQ Sequence 4229 BP; 1500 A; 727 C; 1014 G; 988 T; 0 other; gtacctagga cttgacggag ccatagtatc tcaggaaggt ttcggaaacc cagatacaga 60 ccttataatg aactgcaaga agatagaaat gcaagacgtt aagactgtaa tatctacaga 120 cgagtacgca ggaagagacg gagcttctca gtcgcttgct gatgctgacg ttagagctga 180 cgcagttgta tctaacggaa atgcaaacat ggtaataact cttcctaaga tggacaaggt 240 tataggacat atccagtaca tcgacactat agctggagga ttcgacggat cacttgcaga 300 agacggaagc atagtagtga gatccaggct atacagagct acaacgagct aggatttgga 360 tacctttcag ctaaaggata ctaatattag cctgtaaata gaaaaactca tgctttaata 420 aaatataaat cagaaggagc gactttggta tgtcattatt cgacggcaaa aaagtcatca 480 taatcggtga cagagacgga ataccaggtc ctgctatggc agaatgttta aagggaataa 540 atgtagaagt agtttattca gctactgagt gttttgtctg aacagctgct ggtgctatgg 600 acctagagaa ccaaaactgg gttaaaaact ttacagatca gtatggagca gagaacataa 660 tagttcttgt tggagcagcg gaagctgaat cagcaggact agctgcagag actgtaacag 720 caggagaccc tacattcgca ggtccattag caggagtcca gttgggacta agagtattcc 780 acgcagttga accagaattc aaaggagcag tagactcagc aatatacgat gagcaaattg 840 gaatgatgga aatggttctt gacgttgact ctataataga ggaaatgaag tcaataagag 900 ccgactactg caagttcaac gactaattaa gggaggtaaa atcatgggaa aattaagagt 960 agttcattat ataaaccagt tcttcgcagg aataggtgga gaagaaaaag ctgacatagc 1020 acctttcgta gcagaggagc ttccaccaat aagccaaaac cttgacaaac ttctaggaga 1080 agacgcagaa gtagtagcta cagtagtttg tggagactct ttcttcggag aaaaccttga 1140 aacagcacag gcaacagtac tagaaatggt aaagggtgca agccctgacc tattcatagc 1200 tggaccagca ttcaacgcag gaagatacgg agttgcagct ggagctataa ctaaggctgt 1260 tcaagacaca cttggaatac ctgctgttac aggaatgtac atcgagaacc ctggtgcaga 1320 catgtacaag aagagcgttt acgtaatgtc aacagctgac tcagctgcag gaatgagaaa 1380 gtctcttcca gcgctagcta agttcgctct taagtatgct aagggagagg aaataggatc 1440 tccagcagaa gaaggataca tcgaaagagg aataagagtc aacggattca aagaagacag 1500 aggagctaag agagctgtag ctatgcttgt taaaaagctt aaaggcgaag agttcgtaac 1560 tgagtaccca atgccagttt tcgacaacgt tgtgccagga agagctatag taaacatgtc 1620 aaaagctaaa atagcgatag taacttctgg tggtatagtt ccaaaaggaa acccagatag 1680 aatagagtca tcttcagcat ctaagtacgg aaagtatgac atagacggag tagacgatct 1740 tacttcagaa ggctgggaga cagctcacgg tggacacgac cctgtatacg ctaacgaaga 1800 tgcagacaga gttatacctg ttgacgttct tagagacatg gagaaagaag gagtaatagg 1860 agagcttcac agatacttct actctacaac aggaaacgga actgcagttc taagctctaa 1920 gcagtttgct aaagagttca ctcaagaact aatggcagcc ggagtagacg cagttatact 1980 tacttctacg tgaggcactt gtactcgttg cggcgcaacg atggttaaag aaatagaaag 2040 atcaggaata cctgtagttc acatatgtac agttactcca atagcactta cagttggagc 2100 taacagaata gttccagcaa tagctatacc tcacccacta ggagatccag ctctttctcc 2160 agcagaagag aaggctctta gaagaaagat agttgagaag tctcttaagg ctcttgaaac 2220 agaaatagaa gagcagacag tattcgaaga ctaataagta aacccggggg tagttcattc 2280 tatcctctcc ttaaaaaaca ggtgcgaaag ctttgccttt cacacctgta ataaaaagtt 2340 ttcaaggtat ctttaaaagc tgtaaagata ttttgaaaat gtctttgata agatcgagga 2400 cgcatataat tgtcagaggt tacataaaac aaaacataaa aagcaaacga ttttaaggag 2460 gaagctaaag tggaaaacgt atatgatata gccataatag gttcaggacc tgcaggactt 2520 gcagcagcac tttacggagc aagagcaaag atgaagactc ttcttcttga gggaatgaaa 2580 gttggaggac agatagtaat aactcacgag gttgcaaact atccaggatc agttccagaa 2640 gctacaggac cgtctctaat aggaagaatg gaagagcagg ttgaagaatt cggagcagaa 2700 agagtaatgg acaacatagt agacgttgat tttacagaca agataaaagt acttaaggga 2760 gcaaaaggcg agtacaaggc taaggctgta atagttgcta ctggagcatc tccaaagctt 2820 gcaggatgcc cgggagaaaa agagcttaca ggaaaaggcg tatcatactg cgcgacttgc 2880 gacgctgact tcttcgagga catggaagtg ttcgtaatag gcggaggaga cacagcagtt 2940 gaagaggcta tgttccttac aaaattcgct agaaaagtta caatagttca cagaagagcc 3000 gagcttagag ctgccaagtc tatacaagag aaggcattca aaaacgaaaa gcttaacttc 3060 atgtggaaca cagtaataga agaaataaag ggagacggaa tagtagaatc tgcagtattc 3120 aaaaatagag aaactggaga agttacagag ttcgttgcac ctgaagaaga cggcactttc 3180 ggaatattcg tattcatagg atacgatccc aaaagcgctc ttgtagaagg aaagctagag 3240 cttgacgaga caggctatat accaacagac gacaacatga agactaacgt tgaaggcgta 3300 tttgccgccg gagacataag agtcaagtca cttagacagg tagttacagc gacagctgac 3360 ggagctatag ctgcagttca agctgagaag tatatagaag agctcttcgc agagtaataa 3420 caataagtaa tgcaactaaa aaatgcagtt ataatatgat tgattcagca tggtccattt 3480 ggattgggct gataaattaa taaaaagcaa aaataaaagg agatgaatgc aatgttaatg 3540 ctagacaaag atactttcaa aacagaagtt cttgagggaa caggatatgt actagttgac 3600 tacttcagtg atggatgcgt accatgcaag gctcttatgc cagcggtaga agagctttcg 3660 aagaaatacg aaggaagagt tgtgtttgca aagctcaaca caacaggagc aagaagactt 3720 gctataagcc aaaagatact aggccttcct acactgtctc tatacaagga tggagtaaag 3780 gtggatgaag ttactaaaga cgatgcaaca atagaaaaca tagaggcaat ggtagaagaa 3840 catattagta aataaccagt attatttatg tgtgtaaaag gctgaatctc ataattttga 3900 aaattaaaaa aatacattga aaaacagggc aaagacatgt caaaacggtc tttgctctat 3960 tttgttttta gtggtatcat tgttatgtat ggggattgga aaatttaagg taaggggtgt 4020 ttgttttgaa aaatgaaaaa ttgaaaaagg ggctgatgac cactgtaggt gatatctggc 4080 gcgatttcat atctaattgg agcattggca cttaaagaag cttctcagac ctcaaaaatc 4140 attgtggcgg ctcttggaat ggctacaggt tttttaggat ggacattatc ggaaataaac 4200 acaaggtcct ccataaagca tatagctat 4229 // |
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