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EBI DbfetchID U23955; SV 1; linear; genomic DNA; STD; PRO; 7285 BP. XX AC U23955; XX DT 07-AUG-1995 (Rel. 44, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Corynebacterium sp. serine hydroxymethyltransferase (glyA) gene, partial DE cds, sarcosine oxidase beta (soxB), delta (soxD), alpha (soxA), and gamma DE (soxG) subunit genes, complete cds, and 10-formyltetrahydrofolate hydrolase DE (purU) gene, complete cds. XX KW . XX OS Corynebacterium sp. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Corynebacteriaceae; Corynebacterium. XX RN [1] RP 1-7285 RX DOI; 10.1074/jbc.270.31.18252 RX PUBMED; 7543100. RA Chlumsky L.J., Zhang L., Jorns M.S.; RT "Sequence analysis of sarcosine oxidase and nearby genes reveals homologies RT with key enzymes of folate one-carbon metabolism"; RL J. Biol. Chem. 270(31):18252-18259(1995). XX RN [2] RP 1-7285 RA Chlumsky L.J., Jorns M.S.; RT ; RL Submitted (03-APR-1995) to the EMBL/GenBank/DDBJ databases. RL Lawrence J. Chlumsky, Biological Chemistry, Hahnemann University School of RL Medicine, Broad & Vine Streets, Philadelphia, PA 19102-1192, USA XX FH Key Location/Qualifiers FH FT source 1..7285 FT /organism="Corynebacterium sp." FT /strain="P-1" FT /mol_type="genomic DNA" FT /clone="pLJC305" FT /db_xref="taxon:1720" FT CDS <1..784 FT /codon_start=2 FT /transl_table=11 FT /gene="glyA" FT /product="serine hydroxymethyltransferase" FT /function="conversion of serine to glycine" FT /EC_number="2.1.2.1" FT /note="the sequenced region consists of the FT carboxy-terminal two-thirds of the enzyme" FT /db_xref="GOA:P50434" FT /db_xref="HSSP:1CJ0" FT /db_xref="InterPro:IPR019798" FT /db_xref="UniProtKB/Swiss-Prot:P50434" FT /protein_id="AAC43458.1" FT /translation="IVAGWSAYPRQLDFVRFREIADKVGAYLFVDMAHFAGLVATGLHP FT SPVPHAHVVTSTTHKTLAGPRGGIILSNDAEIAKKLNSAVFPGQQGGPLEHVIAGKAVA FT FKIAASAEFKERQQRTLAGSRILAQRLTQADVAAKGISVLTGGTDVHLVLVDLRHSELD FT GQQAEDLLAKVEITVNRNSVPFDPRPPMTTSGLRIGTPALATRGFSEEAFAEVAEIIAQ FT TLIAGAEGNTGVLPELKARILELAAAHPLYPNLKKIGE" FT CDS 787..2004 FT /codon_start=1 FT /transl_table=11 FT /gene="soxB" FT /product="sarcosine oxidase beta subunit" FT /function="Corynebacterial sarcosine oxidase is a FT heterotetramer that catalyzes the oxidative demethylation FT of sarcosine to yield glycine, hydrogen peroxide and FT 5,10-methylenetetrahydrofolate" FT /db_xref="GOA:P40875" FT /db_xref="InterPro:IPR006278" FT /db_xref="UniProtKB/Swiss-Prot:P40875" FT /protein_id="AAC43459.1" FT /translation="MADLLPEHPEFLWANPEPKKSYDVVIVGGGGHGLATAYYLAKNHG FT ITNVAVLEKGWLAGGNMARNTTIIRSNYLWDESAGIYEKSLKLWEQLPEELDYDFLFSQ FT RGVLNLAHTLGDVRESVRRVEANKFNGVDAEWLTPEQVKEVCPIINIGDDIRYPVMGAT FT YQPRAGIAKHDHVAWAFARKANEMGVDIIQNCEVTGFLKDGEKVTGVKTTRGTIHAGKV FT ALAGAGHSSVLAELAGFELPIQSHPLQALVSELFEPVHPTVVMSNHIHVYVSQAHKGEL FT VMGAGIDSYNGYGQRGAFHVIEEQMAAAVELFPIFARAHVLRTWGGIVDTTMDASPIIS FT KTPIQNLYVNCGWGTGGFKGTPGAGFTLAHTIANDEAHALNAPFSLERFETGHLIDEHG FT AAAVAH" FT CDS 2016..2312 FT /codon_start=1 FT /transl_table=11 FT /gene="soxD" FT /product="sarcosine oxidase delta subunit" FT /db_xref="GOA:Q46336" FT /db_xref="InterPro:IPR006279" FT /db_xref="UniProtKB/Swiss-Prot:Q46336" FT /protein_id="AAC43460.1" FT /translation="MMLIECPNCGPRNETEFSYGGQAHVAYPEDPNALSDKEWSRYLFY FT RENSKGIFAERWVHSGGCRKWFNALRDTATYEFKAVYRAGEPRPELNTQGGSR" FT CDS 2309..5212 FT /codon_start=1 FT /transl_table=11 FT /gene="soxA" FT /product="sarcosine oxidase alpha subunit" FT /db_xref="GOA:Q46337" FT /db_xref="InterPro:IPR006277" FT /db_xref="UniProtKB/Swiss-Prot:Q46337" FT /protein_id="AAC43461.1" FT /translation="MSQNTSYRLPAEQSPAARIDRGEALVLTVDGKQLEAFRGDTVASA FT MLANGQRACGNSMYLDRPRGIFSAGVEEPNALVTVEARHEQDINESMLAATTVPVTANL FT SATLLRGLGVLDPSTDPAYYDHVHVHTDVLVVGAGPAGLAAAREASRSGARVLLLDERA FT EAGGSLRDAAGEQIDGQDAAAWIDATVAELAAAEETTHLQRTTVLGSYDANYVVAVQRR FT TVHLDGPSGAGVSRERIWHIRANQVVLATGAHERPIVFENNDRPGIMLAGAVRSYLNCY FT GVRAGSQIVVATTNDSAYPLVADLAASGGVVAVIDARTTVSAAAAEAVGAGVRVITGSV FT VVDTEANESGELSAVIVAELGEDRELGEPQRFEADVLAVAGGFNPVVHLHSQRQGKLVW FT DTSIHAFVPDAAVANQHLAGALTGLFDTASALSTGAAVGAAAATAAGFERIAQVPQALP FT VPAGETRPVWLVPSLNGDQATNYTTHFVDLQRDQTVSDVLRATGAGLESVEHIKRYTSI FT STANDQGKTSGVAAIGVIAAVLGIENPAEIGTTTFRAPYTPVAFAALAGRTRGELLDPA FT RITPMHSWHLAQGAKFEDVGQWKRAWYFPQDGEDMDAAVYRECAAVRESVGMLDATTLG FT KIEIRGADAAEFLNRIYTNGYTKLKVGMARYGVMCKADGMVFDDGVTLRLAEDRFLMHT FT TTGGAAGVLDWLEEWLQTEWPELDVTCTSVTEQLATVAVVGPRSRDVVAKLVTGLDVSN FT DAFKFMSFQDVTLDSGIEARISRISFSGELAYEIAIPSWHGLRVWEDVYAAGQEFNITP FT YGTETMHVLRAEKGFIIVGQDTDGTVTPQDAGMEWVVSKLKDFVGKRSFSREDNLREDR FT KHLVSVLPVDTALRLAEGAALVADGAVETEGCTPMEGWVTSSYNSPALGRTFGLALIKN FT GRSRIGEVLKTPVNGQLVDVLVSDLVLFDPEGSRRDG" FT CDS 5205..5816 FT /codon_start=1 FT /transl_table=11 FT /gene="soxG" FT /product="sarcosine oxidase gamma subunit" FT /db_xref="GOA:Q46338" FT /db_xref="InterPro:IPR006280" FT /db_xref="UniProtKB/Swiss-Prot:Q46338" FT /protein_id="AAC43462.1" FT /translation="MASNTLIESTSVRRSPAEHLAEAMAQGSTAGTVQLREIAFATQVG FT VRAVPGSGGFAALAEAVGTGLPQQVGVVAGSVEGTAVLWLGPDEFLAIAPEGAELAAEL FT VAALGDEPGQVLDLSANRSVLELSGPAAPLVLRKSCPADLHPRAFGVNLAITTTLANIP FT VLLWRTGEQSWYILPRASFTEHTVHWLIDAMSEFASEPVA" FT CDS 6157..7017 FT /codon_start=1 FT /transl_table=11 FT /gene="purU" FT /product="10-formyltetrahydrofolate hydrolase" FT /db_xref="GOA:Q46339" FT /db_xref="InterPro:IPR004810" FT /db_xref="UniProtKB/Swiss-Prot:Q46339" FT /protein_id="AAC43463.1" FT /translation="MSEQVRHVLTLQCPEGIGIVHAVTGFLVRHQRTIVELKQYDDMSA FT GRLFLRVDFAGDSAPDLLDALRSEFSEVAAKFDMDWQLRERGQKTKVLIMVSKFEHCLQ FT DLLFRMHSGDLPIEVVGVASNHPDHRSLVEWYGIGFHHIPISKDTKPRAEAALLELIDQ FT TGAELVVLARYMQVLSDHLASELTGKTINIHHSFLPSFKGAKPYHQAWERGVKTVGATA FT HYVNSELDEGPIIAQQVVEVDHTYGPQDLVAAGRDSECKALSNAVRWHCEGRVFLYGNR FT TVVLR" FT CDS 7091..>7285 FT /codon_start=1 FT /transl_table=11 FT /product="unidentified" FT /note="unidentified open reading frame" FT /db_xref="UniProtKB/TrEMBL:Q46340" FT /protein_id="AAC43464.1" FT /translation="MASKKRQGKKNSGAGNPAKAAQRGRSVHKIQAELSVDALRGQYVA FT WVAAQVPAFSPADAAQAAEI" XX SQ Sequence 7285 BP; 1250 A; 2527 C; 2412 G; 1096 T; 0 other; gatcgtggcc ggctggtcgg cctaccctcg ccagctcgac ttcgtccgct tccgcgaaat 60 cgcggacaag gtcggtgcgt acctgttcgt ggacatggcg cacttcgccg gcctggtcgc 120 caccgggctg cacccgtcgc cggtgccgca tgcgcatgtg gtcacctcga ccacgcacaa 180 gaccctggca ggccctcgcg gcggcatcat cctgtcgaat gatgcggaga tcgccaagaa 240 gctgaactcg gcggtgttcc ctggccagca gggcggcccg ctggagcatg tgatcgcggg 300 caaggcggtg gcgttcaaga tcgccgcgtc ggcggagttc aaggagcgcc agcagcgcac 360 cttggccggt tcgcggattt tggcgcagcg gttgacccag gccgatgtgg ccgccaaggg 420 catcagcgtg ttgaccggcg gcacggatgt gcacctggtg ctggtggacc tgcgccacag 480 cgagctggat gggcagcagg ccgaggatct gctggcgaag gtggagatca cggtgaaccg 540 caattcggtg ccgttcgacc cgcgtccgcc gatgactacc agcgggttgc ggatcggcac 600 cccggcgttg gcgacccgag gtttctccga ggaggctttt gccgaggttg cggagatcat 660 cgcgcagacc ctgattgccg gggccgaagg caacaccggg gtgttgccgg agttgaaggc 720 ccggattctg gagctggccg cagcccaccc actgtaccca aacctgaaaa agatcggaga 780 atagccatgg ctgatttgct gcctgagcac ccggaattcc tctgggccaa cccggagccg 840 aagaagtcct atgatgtcgt catcgtcggc ggcggcggcc atggcctggc caccgcctac 900 tacctggcca agaaccacgg catcaccaac gtcgccgtcc tggaaaaggg ctggctggcc 960 ggcgggaaca tggcccgcaa caccaccatc atccgctcga actacctgtg ggacgaatcc 1020 gcgggcattt acgagaagtc cctgaagctg tgggagcagc tgcccgagga actggactac 1080 gacttcctgt tctcccagcg cggcgtgctg aacctcgccc acaccctggg agatgtgcgc 1140 gaatcggtgc gccgcgtgga ggccaacaag ttcaacggag tcgacgccga gtggctcacc 1200 ccggaacagg tcaaggaagt ctgcccgatc atcaacatcg gcgacgacat ccgctacccg 1260 gtcatgggcg ccacctacca gccgcgcgcc ggcatcgcca agcacgacca cgtggcctgg 1320 gccttcgccc gcaaggccaa cgaaatgggc gtggacatca tccagaactg cgaagtcacc 1380 ggcttcctga aggacggcga gaaggtcacc ggcgtgaaga ccacgcgcgg caccatccac 1440 gccggcaagg tcgccctggc cggtgccggg cactcctcgg tgctggcgga actggcgggc 1500 ttcgaactgc ccatccagtc ccacccgctg caggccctgg tctccgagct gttcgaaccg 1560 gtccacccga cggtggtcat gtccaaccac atccacgtct acgtctccca ggcccacaag 1620 ggcgaactgg tcatgggcgc gggcatcgac agctacaacg gctatggcca gcgcggcgcg 1680 ttccacgtga tcgaggaaca gatggccgcc gcggtggagc tgttcccgat cttcgcccgg 1740 gcgcacgtgc tgcgcacctg gggcggcatc gtggacacca ccatggacgc ctcgccaatc 1800 atttccaaga cgccgatcca gaacctctac gtcaactgcg gctggggtac cggcggcttc 1860 aagggaaccc cgggcgccgg tttcaccctg gcgcacacca ttgccaacga cgaggcccac 1920 gcgttgaacg caccgttcag cctggaacgt tttgaaaccg gccacctgat cgacgaacac 1980 ggcgcagcag ccgtggccca ctaggagcag ccaccatgat gctgattgaa tgcccgaact 2040 gcgggccgcg caacgaaacc gaattctcct acggcggcca ggcccatgtg gcctaccccg 2100 aggacccgaa cgccctgagc gacaaggaat ggtcccgcta cctgttctac cgcgagaact 2160 ccaaggggat cttcgccgaa cgctgggtcc actccggcgg atgccgcaag tggttcaacg 2220 ccctgcgcga taccgcaacc tatgaattca aggccgtcta ccgggccggg gaaccgcgtc 2280 cggaactgaa cacccaggga ggatcccgat gagccagaac acgtcctacc ggctgccggc 2340 cgagcagtcc ccggccgccc ggatcgaccg cggagaagcc ctggtcctga cggtcgacgg 2400 aaagcagctg gaagccttcc gtggcgacac cgtagccagc gccatgctcg ccaacggcca 2460 gcgcgcctgc ggcaactcga tgtacctgga ccgcccgcgc ggtatcttct ccgccggcgt 2520 cgaggagccc aacgccctgg tgaccgtgga ggcccgccac gagcaggaca tcaacgagtc 2580 gatgctcgcc gccaccaccg ttccggtgac cgccaacctg agcgccacgc tgctgcgcgg 2640 cctgggcgtg ctggacccga gcaccgatcc ggcctactac gaccacgtgc acgtgcacac 2700 cgatgtgctg gtggtaggcg ccggccctgc cggcctggcc gccgcccgcg aggcgtcccg 2760 ctccggggcc cgtgtgctgc tgctggatga gcgcgccgaa gccggaggct cgctgcgcga 2820 tgccgccggc gagcagatcg acggccagga tgccgcagcg tggatcgatg cgaccgtcgc 2880 cgagctggca gcggccgagg aaaccaccca cctgcagcgc accacagtgc tcggctccta 2940 cgacgcgaac tacgtggtcg ccgtgcagcg tcgcaccgtg cacctggatg ggccttccgg 3000 cgccggcgtc tcgcgtgaac gcatctggca catccgcgcc aaccaggtgg tgctggccac 3060 cggcgcccac gagcgcccga tcgtattcga gaacaatgac cgcccgggca tcatgctcgc 3120 cggcgccgtg cgcagctacc tgaactgcta cggcgtgcgc gccgggtcgc agatcgtcgt 3180 ggccaccacc aacgactccg cctacccgct ggttgcggac ctcgccgcca gcggcggagt 3240 ggtcgccgtc atcgatgccc gcaccacggt gtccgccgcg gccgcggaag cagtgggcgc 3300 cggggttcgt gtcatcactg gctccgtggt cgtggacacc gaagccaacg agtcaggtga 3360 actgtcggca gtgatcgtcg ccgagctggg cgaggaccgc gaactgggcg agccgcagcg 3420 cttcgaggcc gacgtgctgg cagtggccgg cggcttcaac ccggtggtgc acctgcactc 3480 ccagcgccag ggcaagctgg tgtgggacac ctcgatccac gccttcgtcc ctgacgcggc 3540 agtggccaac cagcacctgg ccggcgcgct gaccggcctg ttcgacaccg ccagcgcgct 3600 gtccaccggc gccgcggtgg gggccgcggc ggccaccgcc gcgggcttcg agcgcatcgc 3660 ccaggtgccg caggcgctgc cggtgccggc cggggaaacc cgcccggttt ggctggtgcc 3720 ctcgctgaac ggcgaccagg caacgaacta caccacccac ttcgtcgacc tgcagcgcga 3780 ccagaccgtc tccgacgtgc tgcgtgccac cggcgcaggc ctggaatcgg tggagcatat 3840 caagcggtac acctcgatct ccaccgccaa cgaccagggc aagacctcgg gcgtcgcggc 3900 catcggcgtg atcgccgcgg tgctgggcat cgagaacccg gccgagatcg gcaccactac 3960 tttccgcgcc ccgtacaccc cggtcgcctt cgccgccctg gccggccgga cccgcggtga 4020 gctgctggat ccggcgcgca tcaccccgat gcactcctgg cacctggcgc agggcgcgaa 4080 gttcgaggac gtgggccagt ggaagcgcgc ctggtacttc ccgcaggacg gggaagacat 4140 ggacgcggcg gtctaccgcg aatgcgcagc ggtgcgcgag tcggtgggca tgctcgacgc 4200 caccaccctg ggcaagatcg agatccgcgg cgcggatgca gccgagttcc tgaaccgcat 4260 ctacaccaac ggctacacca agctgaaggt cggcatggcc cgctacggcg tgatgtgcaa 4320 ggccgacggc atggtcttcg acgacggcgt gaccctgcgc ctggccgagg accgcttcct 4380 gatgcacacc accaccggcg gcgccgccgg cgtgctggac tggctggagg aatggctgca 4440 gaccgaatgg ccggagctgg atgtcacctg cacctcggtg accgagcagc tggccaccgt 4500 ggcggtggtc ggtccgcggt cgcgcgatgt ggtggccaag ctggtgaccg ggctggacgt 4560 ctccaacgac gcgttcaagt tcatgtcctt ccaggacgtg acccttgatt cgggcatcga 4620 ggcgcgcatc agccgcatct ccttctccgg cgaactggcc tacgagatcg cgatcccgtc 4680 ctggcacggc ctgcgcgtct gggaagacgt gtacgccgcc gggcaggaat tcaacatcac 4740 cccgtacggc actgaaacca tgcacgtgct gcgcgccgaa aagggattca tcatcgtcgg 4800 ccaggacacc gacggcacgg tgaccccgca ggacgccggc atggaatggg tggtctccaa 4860 gctcaaggac tttgtgggca agcgctcctt ctcgcgggaa gacaacctgc gcgaggaccg 4920 caagcacctg gtctcggtgc tgccggtgga caccgcgctg cggctggccg agggcgccgc 4980 cctggtcgcc gacggcgcgg tggaaaccga gggctgcacc ccgatggaag gctgggtcac 5040 cagctcctac aactcgccgg cgctgggccg caccttcggc ctggcactga ttaagaacgg 5100 gcgcagccgc atcggcgaag tgctcaagac cccggtcaac ggccagctgg tcgatgtcct 5160 cgtctccgat ctcgtacttt tcgacccgga agggagccgc cgcgatggct agcaacaccc 5220 tgattgaatc aacctccgtg cgccgttccc cggccgagca cctggccgaa gccatggccc 5280 agggctccac cgccggcacg gtccagctcc gggaaatcgc cttcgccacc caggtcggcg 5340 tgcgcgcggt gcctggcagc ggcggattcg ccgcgctggc agaagccgtc ggcaccgggc 5400 tgccgcagca ggtcggggtg gtcgcaggct ccgtggaggg caccgcggtg ctctggctcg 5460 gaccggacga gttcctggcc atcgcgccgg aaggcgcgga acttgccgca gagctggttg 5520 cggcgctggg ggacgagccc ggccaggttc tggacctttc ggccaaccgc agtgtgcttg 5580 aactgtccgg accggccgcg ccgctggtgc tgcgcaagag ctgcccggcg gacctgcacc 5640 cgcgcgcctt cggcgtcaac ctcgccatca ccaccacgct ggcgaacatc ccggtgctgc 5700 tgtggcgcac cggcgagcag tcctggtaca tcctgccgcg cgcctcgttc accgagcaca 5760 ccgtgcactg gctgatcgac gcgatgagcg aattcgcctc ggagccggtc gcctagcgcg 5820 gcgtagtcct ccattccggg cgctggctgg tttgcggcct gaccggttcc cgccacgtcc 5880 agtgccggga accggtcggt ggcgaccggc gccggcgttg ctcagccggg atgggctgcg 5940 cctcgccgcc gcattgggag gtccggcggc ggagcgttga gcggtccctg cccggcgggt 6000 tgatggttcc cgccgggcac cggggcttca ccggttcagc ccgccgaggg gggagtagcc 6060 tgggagcacc ccgggcgaag aaaccagaca caaacttttt cgcggagcac gccccgagtg 6120 cgtgttccgc cccagacacc tgaatggaag tggatcatga gcgaacaggt tcggcacgtc 6180 ttgacccttc agtgccccga aggcatcggc atcgtgcacg cggtgaccgg cttcctggtc 6240 cggcaccagc gcaccatcgt ggagctcaag cagtacgacg acatgagcgc cggacgcctg 6300 ttcctgcgcg tggatttcgc cggcgactcc gccccggatc tgttggacgc cctgcgcagc 6360 gagttctccg aggtcgccgc gaaattcgac atggactggc agctgcgcga gcgcggccag 6420 aagaccaagg tgctgatcat ggtctccaag ttcgagcact gcctgcagga cctgcttttc 6480 cgcatgcact cgggggacct gcccatcgag gtggtcgggg ttgcttccaa ccacccggac 6540 caccgctcgc tggtggaatg gtacggaatc ggcttccacc acatcccgat cagcaaggac 6600 accaagccgc gggccgaggc cgcgctgctt gaactgatcg atcagaccgg cgccgagctg 6660 gtggtgctgg cccgctacat gcaggtgctc tccgaccact tggccagcga gctgaccgga 6720 aagaccatca acatccacca ttcgttcctg ccatccttca agggcgccaa gccttaccac 6780 caggcgtggg aacgcggcgt gaaaacggtg ggcgccaccg cgcactacgt gaattcggaa 6840 ctcgacgagg gcccgatcat cgcccagcag gtggtggagg tggaccacac ctacggcccg 6900 caggacctgg tggccgccgg ccgcgactcg gaatgcaagg cgctgtccaa cgcggtgcgc 6960 tggcattgcg agggacgggt gttcctctac ggcaaccgca ccgtggtgct gcgctagcat 7020 ccgggtcgca ggaggccagc cgttgcccgt aggcttagtg gcaaacctcc acataagcga 7080 aggacggctc atggccagca agaagcgtca aggcaaaaag aactccggcg caggaaaccc 7140 tgccaaggcg gcccagcgcg gccgcagtgt gcacaagatc caggccgagc tgtcggttga 7200 cgcgctgcgc ggccagtacg tcgcctgggt cgccgcccag gttcctgcct tctctccggc 7260 ggacgcagcc caggccgcgg agatc 7285 // |
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