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EBI DbfetchID U19883; SV 1; linear; genomic DNA; STD; PRO; 5482 BP. XX AC U19883; XX DT 20-FEB-1995 (Rel. 42, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Burkholderia cepacia maleylacetate reductase, glutathione reductase, and DE hydroxyquinol 1,2-dioxygenase genes, complete cds; and unknown genes. XX KW . XX OS Burkholderia cepacia OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex. XX RN [1] RP 1-5471 RX PUBMED; 7538273. RA Daubaras D.L., Hershberger C.D., Kitano K., Chakrabarty A.M.; RT "Sequence analysis of a gene cluster involved in metabolism of RT 2,4,5-trichlorophenoxyacetic acid by Burkholderia cepacia AC1100"; RL Appl. Environ. Microbiol. 61(4):1279-1289(1995). XX RN [2] RP 1-5482 RA Daubaras D.L.; RT ; RL Submitted (14-JAN-1995) to the EMBL/GenBank/DDBJ databases. RL Dayna L. Daubaras, Microbiology/Immunology, University of Illinois at RL Chicago, 835 South Wolcott, Chicago, IL 60612, USA XX FH Key Location/Qualifiers FH FT source 1..5482 FT /organism="Burkholderia cepacia" FT /strain="AC1100" FT /mol_type="genomic DNA" FT /note="tft gene cluster; submitter's given name: FT Pseudomonas cepacia" FT /db_xref="taxon:292" FT CDS 295..1353 FT /codon_start=1 FT /transl_table=11 FT /product="maleylacetate reductase" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF1" FT /db_xref="GOA:Q45072" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:Q45072" FT /protein_id="AAC43333.1" FT /translation="MNAFLFEARIPRVVFGAGALQHLVREIDAMGSTRALVLSTPEQSA FT DAERVAGILGSRAVGVFPRATMHVPIELAREARLEASRLGADCAVAIGGGSTTGLGKAI FT ALESGLPILSIPTTYAGSEMTPIYGVTDNGVKQTGRDARVLPRTVIYDPELTLGLPIRM FT TVSSGLNAIAHAAESLYAHDGNPVIGLMAEEGIRAIGAALTPLQANPADLVARSDALYG FT AWLCGAVLGAVSMGLHHKLCHTLGGAFNLPHAELHTVILPHALAYNSARASQAMERIAR FT ALGVSSAPRGLFDLAERSGLPVSLAALGMPREGIELAADMAVKNPYPNPRPLERDAIRV FT LLEHAYDGVRPS" FT CDS 1392..2741 FT /codon_start=1 FT /transl_table=11 FT /product="glutathione reductase" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF2" FT /db_xref="GOA:P48639" FT /db_xref="HSSP:1ONF" FT /db_xref="InterPro:IPR001327" FT /db_xref="UniProtKB/Swiss-Prot:P48639" FT /protein_id="AAC43334.1" FT /translation="MQKYDFDLFVIGAGSGGVRAARIAAGHGAKVAIAEEYRFGGTCVI FT RGCVPKKLLMYASQYGQGFEDAAGFGWHSAATSHSWTSLIAAKDAEIARLEGVYQRLIE FT NANVEIFKGRAQIAGPNRVTVTGASVSARTILIATGARPVMPPVAGANLMITSDDVFDL FT PVGPPRIAIIGGGYIACEFAGIFNGLGRHVVQLHRGSQVLRGFDDELREHLGDELKKSG FT IDLRLGVDVVAVERQRGALSVQLTTGDAMEVDAVMAATGRLPNTWGLGLETVDVGLDQN FT GAIKVDEYSRTSSPGIYAVGDVTNRLNLTPVAIHEGHAFADTVFGGKALPTEHENVPFA FT VFSQPQAASVGLSEAQARDRYSNVEIYGSAFRPMRAALSGRDEKALVKLVVNGSNDRVV FT GAHIVGADAAEIIQGIAVAIKARATKADFDATLGVHPTLAEEFVTLRNRR" FT CDS 2872..3489 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF3" FT /db_xref="HSSP:1K0D" FT /db_xref="InterPro:IPR004046" FT /db_xref="UniProtKB/TrEMBL:Q45073" FT /protein_id="AAC43335.1" FT /translation="MIELYTWGTPNGRKVSIALEELGLAYNVHPVDIMKGDQFTAGFLA FT LNPNNKIPVIKDSDSLDGQPITLFESGAILVYLADKTQRLLPVSGIARYETLQWLMFQM FT GGIGPMFGQTHHFRRYAVQEKYSLDRYTAETHRLYGVLNKRLAQSEFVAGKEYSIADIA FT IYPWVSRFELHQLDWADVPHVRRWYDAVGARPAVRRGMGVLS" FT CDS 3486..3980 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF4" FT /db_xref="HSSP:1AW9" FT /db_xref="InterPro:IPR010987" FT /db_xref="UniProtKB/TrEMBL:Q45074" FT /protein_id="AAC43336.1" FT /translation="MTNDTMLTVLGRATSSNVQKVMWLLDEIGQPCVRVDMGGQFGGNK FT EAEYLQKNPNGVVPTLIDMDTVVWESNTILRYLSNRFGAFDLYPVDPVHRAWIERWMDW FT QLSTLGPANTLLFQSIVRTPPDKRSADLIESYRERNAKLFDVLDQVLAGRRYLGGTRDA FT R" FT CDS 4199..4501 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF5" FT /db_xref="HSSP:1MWQ" FT /db_xref="InterPro:IPR005545" FT /db_xref="UniProtKB/TrEMBL:Q45075" FT /protein_id="AAC43337.1" FT /translation="MLFLIYRKDRPGSLQVRIDNYAAHLAYLEPLKAKIQVGGPTLGAG FT TGTDDKDMTGSFLIMEAESWDEVHSFVENDPFTKAGLFAATIVERWKHGKHNDSK" FT CDS 4579..5460 FT /codon_start=1 FT /transl_table=11 FT /product="hydroxyquinol 1,2-dioxygenase" FT /function="involved in the degradation of FT 2,4,5-trichlorophenoxyacetic acid" FT /note="ORF6" FT /db_xref="GOA:Q45076" FT /db_xref="InterPro:IPR015889" FT /db_xref="UniProtKB/TrEMBL:Q45076" FT /protein_id="AAC43338.1" FT /translation="MRNLNQDNITQAFLARLVNMPDNRLKTILVNLVQHLHTFARETKL FT TEEEWKQGIDFLTATGHKCNETRQEFILLSDTLGLSQLVVAQNHQRSSGATEQTVFGPF FT HVPGCADSAPHGADITNGAVGEPCFVSVRVLAANGTPLAAATVDCWQADSEGFYDVQDS FT EWAQDNMSLRARFVTDESGDLSMRTVLPKSYPIPTDGTVGQMLEATHRSPMRPAHIHFM FT VQKPGYDTLVTHIFAEGDEYLDSDAVFGVRSSCIREYVKHAPGIAPDGTKMNEPFYTMQ FT CDLVLEPLAAAA" XX SQ Sequence 5482 BP; 1143 A; 1500 C; 1752 G; 1087 T; 0 other; ctgcaggggt tgataccggg gctgaccgct ctcgagatcg gagtcgatgt cagtgcgatt 60 gaatacgcat gtgacgtcgt gctttactcg gagttcgagt cgagagacgc gctcgatgca 120 tacgcgaccc atcccgagca tttgcgggtc aagcgggaac tggccgatct gagaatcgcg 180 cgctatcagg tcgactacgt cgtggactga tggccgcgcg agtgaacatc actctgttcg 240 agcaatgagg cggctgctcg ccgcctgatc accttatcaa cggaccggca tattgtgaac 300 gcttttctct tcgaagccag aatcccgcgg gtcgtatttg gcgccggcgc gttgcaacac 360 ctggtgcggg aaatcgatgc gatgggatcc acccgcgcac tggttttgtc gaccccggag 420 caatcggccg atgccgaacg ggtcgccggg atcctcggga gtcgcgcggt tggagtattt 480 cccagagcga ccatgcacgt gccgatcgaa ctcgcgaggg aagcgcgtct cgaagcgtcg 540 cgactcggcg ctgactgcgc cgtggccatt ggcggaggct cgacaacggg cctaggaaaa 600 gccatcgcgc tcgaaagcgg gttgcctatc ctgtcgatcc cgacaaccta tgcgggtagt 660 gaaatgacgc ccatctacgg cgtcacggac aacggcgtga agcagaccgg ccgcgacgcg 720 cgcgttctgc cgcgtaccgt catctacgac ccggagttga cccttggcct gccgatccgc 780 atgacggtgt ccagtggact caacgcgatt gcgcacgcgg ccgagagcct gtacgcccat 840 gacggcaatc cagtcatcgg actgatggcg gaagagggga ttcgcgccat tggggcggcg 900 ctcacgccgc tgcaggcaaa cccggcggat cttgtcgcgc gcagcgatgc gctgtacggg 960 gcgtggctat gtggtgcggt cctcggtgcg gtgtctatgg gcctgcatca caagctgtgt 1020 catacgttag gcggagcctt caatctgcct cacgcggagt tgcacaccgt aatactcccg 1080 catgccctcg cctataacag cgcgcgtgcc tcccaggcga tggagcgcat tgcacgtgcg 1140 ttgggcgtgt ccagtgcgcc ccgcggatta ttcgatctcg ccgaacgcag cggactaccg 1200 gtttcgctgg cggcgctcgg catgccgcgc gaaggcatag agctcgctgc agacatggcc 1260 gtcaagaacc cgtatccgaa tccacgcccc cttgaacggg acgcgataag ggtgctgctg 1320 gagcatgcat acgacggcgt gcgtccatca tgatggttcg cgtttgtagc cacaacgaat 1380 agcgggtatc catgcaaaag tacgattttg atctctttgt gatcggagca ggctcgggtg 1440 gcgtaagagc cgcccgcatc gccgctgggc atggcgcgaa agttgccatt gctgaggaat 1500 accgctttgg cggaacctgc gtgattcggg gctgcgtccc gaagaagctg ttgatgtatg 1560 cgtcgcagta cggacaaggc ttcgaggacg cggcgggatt cggctggcac tcggcggcca 1620 cgagtcatag ctggacttcg ctgatcgcgg caaaggacgc cgaaatagcc cggcttgaag 1680 gcgtttatca acgtttgatt gagaacgcca acgtagaaat cttcaaagga cgggcgcaga 1740 ttgccggtcc gaatcgcgtc accgtgactg gcgcttcagt gtccgccaga acgatcctga 1800 tcgcgacggg cgcgaggccc gtcatgccac cggtcgcggg cgcgaacctg atgataacgt 1860 cggacgatgt gtttgatctg cctgtcggcc cgccccggat cgcgatcatc ggaggcggct 1920 acatcgcctg cgagttcgcc gggatattca acgggcttgg gaggcacgtg gtgcagcttc 1980 atcgcggatc gcaggttctg cgtggattcg atgatgagct aagggagcac ctgggtgacg 2040 agctcaagaa aagcggcata gacctgcgcc tgggtgttga cgtcgtggcc gtcgaacggc 2100 agcgcggcgc actgtctgtt cagttgacca cgggagacgc tatggaggta gatgccgtca 2160 tggctgcgac gggaaggctg cccaatacat ggggacttgg actcgagacg gttgatgtag 2220 gactggacca gaatggtgcg atcaaggttg acgaatattc ccgaacgtcg agtccgggca 2280 tctacgcggt cggcgacgta acgaaccgcc tgaacctgac accggttgcg atccatgagg 2340 gacatgcgtt tgccgacacc gtcttcgggg gcaaagcgct cccgaccgaa cacgaaaacg 2400 tgcctttcgc cgtattcagc caaccccagg cggcatcagt ggggctgagc gaagctcagg 2460 cgcgcgaccg ctattcgaac gtcgagattt atggcagcgc gtttcgcccg atgcgtgcgg 2520 cgttgtcagg gcgcgacgag aaggcgctgg tgaaactggt ggtcaacgga agcaacgatc 2580 gggtagtggg tgcacatatc gtcggcgccg atgcggcaga gatcattcag ggaatcgctg 2640 ttgcgataaa ggctcgggca acgaaggcgg atttcgacgc aacgctgggc gttcatccga 2700 cactggccga ggaattcgtc accctgcgta accgacggtg agccgttggg ttgtaaaact 2760 gggaagctgt ttcccgcatg cgatgttgtt ggcgcccggg cgactctcta cacttaatag 2820 accgagccgc gattgccgtg ctggaaagaa agacgttctg gagcagattg aatgatcgaa 2880 ctttatacgt gggggactcc taacggccga aaggtgtcga ttgcgctgga ggagttagga 2940 ttggcctata acgtgcaccc agtcgacatc atgaagggtg atcaattcac tgccgggttt 3000 ctggcattga atccgaacaa caagattccg gtcatcaagg acagcgatag tctggacgga 3060 cagccgatta cgttgttcga gtccggagcg atacttgtct atctggcgga caagacgcag 3120 cgcctgctgc cggtgtcagg catcgcgcgc tatgagactc tccagtggct gatgtttcag 3180 atgggcggga tcgggcccat gttcggtcag acgcatcact tccggcgtta cgccgtgcag 3240 gagaagtaca gtctcgatcg ctacacggcc gagacgcacc ggctgtacgg tgtgctgaac 3300 aagagactgg cacaaagcga attcgtggcc ggaaaagagt acagcatcgc ggacatcgca 3360 atctatccct gggtttcgcg ctttgagctc catcagctcg actgggctga tgttccgcat 3420 gtcaggcgct ggtatgacgc ggtaggtgcg aggccagccg tgaggcgagg catgggggtg 3480 ctttcatgac gaacgacacg atgctgacgg tactggggcg ggcaacttcg tccaacgtgc 3540 agaaggtcat gtggctgctg gatgaaatcg gtcagccctg tgttcgcgtc gacatgggtg 3600 ggcaattcgg cggcaacaag gaagcggagt atctgcaaaa gaatccgaac ggtgtcgtgc 3660 cgactttgat cgatatggat accgtggtct gggagtccaa taccatcctg cgctacctgt 3720 cgaaccggtt cggagcgttt gatctgtatc cagtcgaccc ggtacatcga gcctggatag 3780 agcgatggat ggactggcag ttgtcgacgt tgggcccggc aaatacgctg ctgttccaga 3840 gcatcgtacg cacgccacct gacaagcgct cggcagatct aatcgaaagc taccgggaac 3900 gaaatgcaaa actgtttgac gttctcgacc aggtgctggc tggtcgaaga tacttggggg 3960 ggacgcgtga cgctcgctga cattgcactc gggggcttgg cttatcgttg gttcaactgg 4020 ccggtcgagc ggcgcggata taaggatctt cggcgctggt atgacttgat ttgccaacgc 4080 cctgcatttc agcagcacat cgtaaagatt ggattgagtt aggtcgggta cgtcaaccgt 4140 tctaccggga cgccttctct gcttcctgtg cggacacaac caatgttaag gagcgaacat 4200 gctatttttg atctaccgta aagacaggcc ggggagtctt caagtccgaa tcgataacta 4260 tgcagcccac cttgcctacc tggagccgct caaagcaaag attcaggtag gcggaccgac 4320 acttggcgcc ggaacgggaa ccgacgacaa ggacatgacg ggcagcttcc tgatcatgga 4380 ggcggaaagt tgggacgagg tgcacagctt cgtcgagaac gacccgttca cgaaagctgg 4440 actgtttgcc gcgacgatcg tcgaacgctg gaagcacggc aagcacaacg actccaaatg 4500 acaaggtgcc ggtgcccgtc gtcttgaccg ggccggtatc tgtctctcac tgaaaccaca 4560 aaatcgagga ggtatgcaat gcgcaacctc aaccaagaca acatcacgca ggcgtttctc 4620 gcacggctgg tcaacatgcc ggacaatcgc ctaaagacga ttctggtcaa cttggttcag 4680 catctccata cgtttgcgcg ggagactaag ctgacagaag aggaatggaa gcagggcatc 4740 gatttcctga cggcgacggg tcacaaatgc aatgagacgc ggcaggaatt catcctgctg 4800 agcgacacgc tcggcctctc gcaactggtg gtggcgcaga accaccagcg ttcgagcggc 4860 gcgacggagc agaccgtgtt cggccccttc catgtgccgg ggtgcgccga ctctgccccg 4920 cacggtgcgg atatcaccaa tggtgcggtg ggtgaaccgt gcttcgtatc ggtacgcgta 4980 ttggcggcga atggcacacc tctggcagct gcgacagtcg actgctggca ggcggattcc 5040 gaaggctttt acgacgttca ggattcggag tgggcgcagg acaacatgtc gctgcgcgcc 5100 cgcttcgtca ccgatgagtc cggcgatctg tcgatgcgca cggtgttacc gaaaagctat 5160 ccgattccga ctgacggaac ggtgggtcaa atgcttgagg cgacgcatcg ctcgccgatg 5220 cgcccagccc atatccactt catggtgcag aagccgggct acgacacgct ggtgacgcac 5280 atatttgcag aaggcgacga atacctggac tccgatgctg tgtttggggt gcgatcgtca 5340 tgcattcggg aatatgtgaa gcacgcgccg ggcatcgcac cggacggcac caaaatgaac 5400 gagcctttct atacgatgca gtgcgacctc gttcttgagc cacttgcagc agcagcttga 5460 agcagccgga ggggttgtgg gt 5482 // |
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